• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.835-842
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• 2014

Haloperoxidase (HPO, E.C.1.11.1.7) is a metal-containing enzyme oxidizing halonium species, which can be used in the synthesis of halogenated organic compounds, for instance in the production of antimicrobial agents, cosmetics, etc., in the presence of halides and $H_2O_2$. To isolate and evaluate a novel non-metal HPO using a culture-independent method, a cassette PCR library was constructed from marine seawater in Japan. We first isolated a novel HPO gene from Pseudomonas putida ATCC11172 by PCR for constructing the chimeric HPO library (HPO11172). HPO11172 showed each single open-reading frame of 828 base pairs coding for 276 amino acids, respectively, and showed 87% similarity with P. putida IF-3 sequences. Approximately 600 transformants screened for chimeric genes between P. putida ATCC11173 and HPO central fragments were able to identify 113 active clones. Among them, we finally isolated 20 novel HPO genes. Sequence analyses of the obtained 20 clones showed higher homology genes with P. putida or Sinorhizobium or Streptomyces strains. Although the HPO A9 clone showed the lowest homology with HPO11172, clones in group B, including CS19, showed a relatively higher homology of 80%, with 70% identy. E. coli cells expressing these HPO chimeric genes were able to successfully bioconvert chlorodimedone with KBr or KCl as substrate.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.887-892
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• 2015

Uricase is an important microbial enzyme that can be used in the clinical treatment of gout, hyperuricemia, and tumor lysis syndrome. A total of 127 clinical isolates of Pseudomonas aeruginosa were tested for uricase production. A Pseudomonas strain named Ps43 showed the highest level of native uricase enzyme expression. The open reading frame of the uricase enzyme was amplified from Ps43 and cloned into the expression vector pRSET-B. Uricase was expressed using E. coli BL21 (DE3). The ORF was sequenced and assigned GenBank Accession No. KJ718888. The nucleotide sequence analysis was identical to the coding sequence of uricase gene puuDof P. aeruginosa PAO1. We report the successful expression of P. aeruginosa uricase in Escherichia coli. E. coli showed an induced protein with a molecular mass of about 58 kDa that was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. We also established efficient protein purification using the Ni-Sepharose column with activity of the purified enzyme of 2.16 IU and a 2-fold increase in the specific activity of the pure enzyme compared with the crude enzyme.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.26 no.10B
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• pp.1382-1389
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• 2001

움직임 보상-이산 코사인 변환 (motion compensation-discrete cosine transform : MC-DCT) 기반의 동영상 부호화 기법이 부호화 효율성 및 구현의 단순성으로 인해 널리 사용되고 있으나, 에러 환경에서 구조적으로 취약한 면이 있다. 본 논문에서는 다중 메모리 움직임 보상 예측 (long-term memory motion compensated prediction : LTMP) 기반의 다중 레프런스 프레임을 사용하여 에러에 강인한 동영상 부호화 기법을 제안한다. 또한 제안하는 알고리듬에 기반한 에러 은닉 기법 (error concealment : EC)을 구현한다. 즉, R-D (rate-distortion) 최적화에 프레임간 움직임 벡터 (temporal motion vectors)의 확산 인자를 추가하여 에러에 대한 강인성 및 에러 은닉 기법의 효율성을 증가시켰다. 또한, 제안하는 알고리듬은 시간축상의 에러 전파를 피드백 정보 (negative acknowledgement : NAK)를 사용하여 억제한다. 즉, NAK는 채널 에러에 의해 손실된 영역과 에러가 전파된 영역을 추정하여 움직임 보상 영역에서 제외되도록 하는데 이용된다. 따라서, 제안하는 알고리듬은 PSNR 측면에서 FIU (forced intra update)에 근사하는 성능을 보이나, FIU와는 달리 비트율의 증가를 피할 수 있어 제한된 대역폭의 네트웍을 효율적으로 사용할 수 있다. 컴퓨터 모의 실험을 통해 제안하는 알고리듬이 기존의 H.263 및 LTMP 기반의 부호기에 비해 에러 환경에서 주관적 및 객관적 화질 측면에서 성능이 우수함을 보인다.

• Journal of Bio-Environment Control
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• v.1 no.1
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• pp.37-51
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• 1992

Quantizing and extracting visual features of mushroom(Lentinus edodes L.) are crucial to the sorting and grading automation, the growth state measurement, and the dried performance indexing. A computer image processing system was utilized for the extraction and measurement of visual features of front and back sides of the mushroom. The image processing system is composed of the IBM PC compatible 386DK, ITEX PCVISION Plus frame grabber, B/W CCD camera, VGA color graphic monitor, and image output RGB monitor. In this paper, an automatic thresholding algorithm was developed to yield the segmented binary image representing skin states of the front and back sides. An eight directional Freeman's chain coding was modified to solve the edge disconnectivity by gradually expanding the mask size of 3$\times$3 to 9$\times$9. A real scaled geometric quantity of the object was directly extracted from the 8-directional chain element. The external shape of the mushroom was analyzed and converted to the quantitative feature patterns. Efficient algorithms for the extraction of the selected feature patterns and the recognition of the front and back side were developed. The developed algorithms were coded in a menu driven way using MS_C language Ver.6.0, PC VISION PLUS library fuctions, and VGA graphic functions.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.309-316
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• 1990

The sequence of a 1795 bp restriction fragment containing the B. circulans F-2 gene for NaC1- dependent $\alpha$-amylase (CI-amylase) is reported. The probable coding region of the gene is 1005 base pairs (335 amino acida) long. The NaC1-dependent $\alpha$-amylase (el-amy) sequence shows an open reading frame (ORF) with the translated molecular weight of about 38, 006, which correspond to a molecular weight of about 35, 000 (Mi). The gene is preceded by the sequence resembling promoter for the vegetative B, subtitis RNA polymerases. These are followed by the sequences resembling a B. subtilis ribosome binding site 5 nucleotides before the first codon of the gene. Homologous regions with other amylases were found. The N-terminal sequences of the mature proteins expressed in E. eoli were identical to the N-terminal sequences which are anaIysed.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.4 no.4
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• pp.286-294
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• 2011

Cable modems typically are implemented by a forward error correction(FEC) scheme. The ITU-T Recommendation J-38 Annex B specifies using 64- and 256- quadrature amplitude modulation (QAM) and extended RS coding scheme. In implementing the cable modem, there are some problems to fabricate and fitting on FPGA chip. First, many clocks are needed in implementing cable modem because of different code rate and different modulation types. To reduce the number of clocks, we use the two memories, which are different clock speed for reading and writing data. Second, this system lost the bit-synchronization and frame-synchronization in decoder, the system recognize that all data is error. This paper solves the problems by using simple 5-stage registers and unique sync-word. Based on solutions for about problems, the cable modem is fabricated on FPGA chip name as Vertex II pro xc2vp30-5 by Xilinx, and we confirmed the effectiveness of the results.

• KSBB Journal
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• v.10 no.5
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• pp.499-509
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• 1995

PU.1, a tissue-specific transcription activator, binds to a purine-rich sequence(5'-GAGGAA-3') called PU box. The PU.1 cDNA consists of an open reading frame of 816 nucleotides coding for 272 amino acids. The amino terminal end is highly acidic, while the carboxyl terminal end is highly basic. Transcriptional activation domain is located at the amino terminal end, while DNA binding domain is located at the carboxyl terminal end. Activation of PU.1 transcription factor is supposed to be accomplished by the phosphorylation of serine residue(s). There exist 22 serines in the PU.1. Five(the 41, 45, 132$.$133, and 148th) of the serines(plausible phosphorylation site by casein kinase II), are the primary targets of interest in elucidating the molecular mechanism(s) of the action of the PU.1 gene. In this study, PU.1 cDNA coding for the five serine residues(41th AGC, 45th AGC, 132$.$133th AGC$.$TCA, and 148th TCT), was mutated to alanine codon(41th GCC, 45th GCC, 132$.$133th GCC$.$GCA, and 1481h GCT), respectively, by Splicing-Overlapping-Extension(SOE) using Polymerase Chain Reaction(PCR). And each mutated cDNA fragments was ligated into pBluescript KS＋ digested with HindIII and Xba I, to generate mutant clones named pKKS41A, pRKS45A, pMKS132$.$133A, and pMKS148A. The clones will be informative to study the "Structure and Function" of the immu-nologically important gene, PU.1.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.8
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• pp.2010-2024
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• 1998

In video coding, it is important to improve the average picture quality as well as to maintain cosistent picture quality between consecutive pictures. In this paper, we propose a distortion-rate estimation method for MPEG-2 video and a forward rate control method, using the proposed estimation result, to be able to obtain the improved and consistent picture quality of CBR (Constant Bit Rate) encoded MPEG-2 video. The proposed distortion-rate estimation enable us to predict the distortion and the bits generated from an encoded picture at a given quantization step size and vice versa. The most attactive features of proposed distortion-rate estimation are its accuracy and low computational complexity enough to be applied to the practical video coding. In addition, the proposed rate control first determined a quantization parameter per frame by following procedure: distortion-rate estimation, target bit allocation, distortion constraint and VBV(Video Buffer Verification) constraint. And then this quantization parameter is applied to the encoding so that improved and consisten picture quality can be obtained. Furthermore the proposed rate control method can solve the error propagation problem caused by scene change or anchor picture degradation by using the B-picture skipping and the guarantee of the minimum bit allocation for the anchor picture. Experimental results, comparing the proposed forward rate control method with TM5 method, show that the proposed method makes more improed and consistent picture quality than TM5.

• Korean Journal of Agricultural Science
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• v.34 no.2
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• pp.161-170
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• 2007

To characterize $\gamma$-glutamyltranspeptidase ($\gamma$-GTP or ggt; EC 2. 3. 2. 2.) gene of Bacillus subtilis BS 62, the $\gamma$-GTP gene of BS 62 was prepared from PCR products amplified with the chromosomal DNA. The $\gamma$-GTP gene of about 2.5 kb was sequenced, and its homology was compared with the other ggt genes which were reported previously. The base sequence of the gene appeared to have an open reading frame of 1,758 bp encoding a protein of 62,175 Da. The coding region was flanked by putative ribosome binding site - AGGAGG of 7th to 12th upstream - and the stem-loof sequence was followed by transcription terminator codon. Homology of the amino acid residues sequence consisting of 587 amino acid residues was found as 98% with Bacillus subtilis gene (BSU49358), 97.4% with that of Bacillus subtilis KX 102, 37% with Pseudomonas sp. A14 (S63255) and 38% with Streptomyces avermitils (AP005028).

• The KIPS Transactions:PartB
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• v.10B no.1
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• pp.95-102
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• 2003

In surveillance video system, there are many classes of images and some spatial regions are more important than other regions. The conventional compression method in this system have been compressed there full frames without classfying them depend on their important parts. To improve the accuracy of the image coding and deliver effective compression for the surveillance video system, it was necessary to separate the regions according to their importance. In this paper, we propose a new effective surveillance video image compression method. The proposed scheme defines importance based three-level region of interest block in a frame, such as background, motion object block, and the feature object block. A captured video image frame can be separated to these three different levels of block regions. And depends on the priority, each block can be modified and compressed in different resolution, compression ratio and qualify factor. Therefore, in surveillance video system, this algorithm not only reduces the image processing time and space, but also guarantees the Important image data in high quality to acquire the system's goal.