• Proceedings of the Microbiological Society of Korea Conference
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• 2004.05a
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• pp.126-126
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• 2004

• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.679-685
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• 2011

Xanthomonas oryzae pv. oryzae (Xoo) produces a putative effector, XoAvrBs2. We expressed XoAvrBs2 homologously in Xoo with a TAP-tag at the C-terminus to enable quantitative analysis of protein expression and secretion. Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels, and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium. In a T3SS-defective Xoo mutant strain, secretion of the TAPtagged XoAvrBs2 was blocked. Thus, we elucidated the transcriptional and translational expressions of the XoAvrBs2 gene in Xoo was induced in vitro by the interaction with rice and the induced secretion of XoAvrBs2 was T3SSdependent. It is the first report to measure the homologous expression and secretion of XoAvrBs2 in vitro by rice leaf extract. Our system for the quantitative analysis of effector protein expression and secretion could be generally used for the study of host-pathogen interactions.

• The Plant Pathology Journal
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• v.20 no.1
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• pp.7-12
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• 2004

An important recent advance in the field of plant-microbe interactions has been the cloning of genes that confer resistance to specific viruses, bacteria, fungi or insects. Disease resistance (R) genes encode proteins with predicted structural motifs consistent with them having roles in signal recognition and transduction. Plant disease resistance is the result of an innate host defense mechanism, which relies on the ability of plant to recognize pathogen invasion and efficiently mount defense responses. In tomato, resistance to the pathogen Pseudomonas syringae pv. tomato is mediated by the specific recognition between the tomato serine/threonine kinase Pto and bacterial protein AvrPto or AvrPtoB. This recognition event initiates signaling events that lead to defense responses including an oxidative burst, the hypersensitive response (HR), and expression of pathogenesis- related genes.

• Transactions on Electrical and Electronic Materials
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• v.17 no.6
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• pp.341-350
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• 2016

The current study is dedicated to design a novel coordinated controller to effectively increase power system rotor angle stability. In doing so, the coordinated design of an AVR (automatic voltage regulator), PSS2B, and TCSC (thyristor controlled series capacitor)-based POD (power oscillation damping) controller is proposed. Although the recently employed coordination between a CPSS (conventional power system stabilizer) and a TCSC-based POD controller has been shown to improve power system damping characteristics, neglecting the negative impact of existing high-gain AVR on the damping torque by considering its parameters as given values, may reduce the effectiveness of a CPSS-POD controller. Thus, using a technologically viable stabilizer such as PSS2B rather than the CPSS in a coordinated scheme with an AVR and POD controller can constitute a well-established design with a structure that as a high potential to significantly improve the rotor angle stability. The design procedure is formulated as an optimization problem in which the ITSE (integral of time multiplied squared error) performance index as an objective function is minimized by employing an IPSO (improved particle swarm optimization) algorithm to tune adjustable parameters. The robustness of the coordinated designs is guaranteed by concurrently considering some operating conditions in the optimization process. To evaluate the performance of the proposed controllers, eigenvalue analysis and time domain simulations were performed for different operating points and perturbations simulated on 2A4M (two-area four-machine) power systems in MATLAB/Simulink. The results reveal that surpassing improvement in damping of oscillations is achieved in comparison with the CPSS-TCSC coordination.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.828-830
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• 2003

AVR은 동기발전기의 출력전압을 부하변동에 관계없이 일정하게 제어하며 발전기 및 시스템 보호를 위한 각 종 제어 알고리즘을 구비하고 있다. 종래의 아나로그타입 AVR은 제어설정이 불편하고 제어신뢰성이 떨어지기 때문에 점차 디지털 타입으로 교체되고 있다. 본 연구는 선박발전기에 적용하기 위한 디지털 AVR 개발에 관한 것이다. TI사의 TMS320LF2407 CPU를 사용하여 제어 H/W를 개발했으며, 과여자/저여자제어, Droop제어, 무부하/역률제어 등 다양한 제어기능을 S/W로 구비하여 부하에 따른 적용의 유연성을 높였다. 그리고 전력회로는 IGBT를 사용하여 고속 스위칭 제어함으로써 SCR 방식에 비해 제어 속응성을 개선하였다. 개발 된 제품은 AVR, 직류전원공급장치 및 부하나 계통의 사고시 보호를 위한 전류부스트(CBS)기능을 포함하고 있다.

• Proceedings of the KIEE Conference
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• 1992.07b
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• pp.710-713
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• 1992

An AVR for OLTC controls motor tap in transformer in order to supply constant power source. Recently for society which is higher informative and more automatic, in our research, the digital AVR for OLTC is developed that can perform more functions and higher functions ( mesurement, display, protection, control ) using microprocessor in a different control method than the conventional AVR. The experiment result is also present.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.235-240
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• 2007

AvrRpt2 protein triggers hypersensitive response (HR) and strong disease resistance when it is translocated from a bacterial pathogen Pseudomonas sp. to host plant cells containing a cognate RPS2 resistance protein through Type III Secretion System (TTSS). However, AvrRpt2 protein can function as the effector that suppresses a basal defense and enhances the disease symptom when functional RPS2 resistance protein is absent in the infected plant cells. Using Affymetrix Arabidopsis DNA chip, we found that many genes were specifically regulated by AvrRpt2 protein in the rps2 Arabidopsis mutant. Here, we showed that expression of AtERF11 that is known as a member of B1a subcluster of AP2/ERF transcription factor family was down regulated specifically by AvrRpt2. To determine its function in plant resistance, we also generated the Arabidopsis thaliana transgenic plants constitutively overexpressing AtERF11 under CaMV 355 promoter, which conferred an enhanced resistance against a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, these results collectively suggest that AtERF11 plays a role as a positive regulator for disease resistance against biotrophic bacterial pathogen in plant.

• Proceedings of the KIPE Conference
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• 2005.07a
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• pp.317-320
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• 2005

본 논문은 멀티-레벨 H-bridge 인버터에서 입력전압 변동에 따른 AVR(Auto Voltage Regulation)기능을 적용 그 타당성을 제안하였다. 기존의 범용 인버터에서 (V/F)로 구동되는 전동기 시스템에 있어서 인버터에 공급되는 입력 전압은 출력 주파수에 따라 출력 전압 비율을 일정하게 하고 기동에 필요한 전압을 더하여 출력하는 방식이다. 집중 제어 방식의 멀티-레벨 H-bridge 인버터에서는 Cell의 DC-Link 전압을 Master에서 받아들여서 각각의 Cell에 기준 전압값을 지령하게 된다. 그러므로 입력 전압 변동에 따른 DC-Link 전압의 변동이 발생하게 되면 상전압 Unbalance 가 발생하게 되어 부하가 원하는 출력 전압을 낼 수가 없게 된다. 또한 각각의 Cell을 제어하는 Master 제어기가 가지고 있는 문제점을 보완하여 각각의 Cell 제어기 스스로가 AVR을 수행하는 좀더 나은 방법을 제안하였다.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.135.2-136
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• 2003

To characterize plasmids in Xanthomonu axonopodis pv. glycines, we isolated plasmids pAG1 from the strain AG1 and pXAG81 and PXAG82 from the strain Bra, respectively, and sequenced three plasmids. The size of plasmids, pAG1, pXAG81, and pXAG82 was 15,149-base pairs (bp), 26,727-bp, and 1,496-bp, respectively Fifteen and twenty six possible open reading frames (ORFs) were present in pAG1 and pXAG81, respectively. Only one ORF homologous to a rep gene of Xylella fastidiosa was present in pXAG82. pAG1 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, three parA genes, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, two parA genes, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven hypothetical proteins. Based on DNA sequence analysis, we presume that pXAG81 is a conjugal plasmid. Interestingly, we found 0.5-kb truncated avirulence gene similar to aurXacE3 on the right border of avrBs3 homolgs of pAG1 and pXAG81. Two hundred twenty five isolates were analyzed to find aurBS3 or tra gene homologs by Southern hybridization. The numbers of avrBs3 homolog varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to can conjugative plasmids (pXAG81 type), and thirty eight isolates appeared to carry non-conjugative plamids (pAGl type). This indicated that aurBs3 gene homologs might be spread by conjugation in X. axonopodis pv. glycines.

• Molecules and Cells
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• v.42 no.7
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• pp.503-511
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• 2019

As sessile organisms, plants have developed sophisticated system to defend themselves against microbial attack. Since plants do not have specialized immune cells, all plant cells appear to have the innate ability to recognize pathogens and turn on an appropriate defense response. The plant innate immune system has two major branches: PAMPs (pathogen associated molecular patterns)-triggered immunity (PTI) and effector-triggered immunity (ETI). The ability to discriminate between self and non-self is a fundamental feature of living organisms, and it is a prerequisite for the activation of plant defenses specific to microbial infection. Arabidopsis cells express receptors that detect extracellular molecules or structures of the microbes, which are called collectively PAMPs and activate PTI. However, nucleotidebinding site leucine-rich repeats (NB-LRR) proteins mediated ETI is induced by direct or indirect recognition of effector molecules encoded by avr genes. In Arabidopsis, plasmamembrane localized multifunctional protein RIN4 (RPM1-interacting protein 4) plays important role in both PTI and ETI. Previous studies have suggested that RIN4 functions as a negative regulator of PTI. In addition, many different bacterial effector proteins modify RIN4 to destabilize plant immunity and several NB-LRR proteins, including RPM1 (resistance to Pseudomonas syringae pv. maculicola 1), RPS2 (resistance to P. syringae 2) guard RIN4. This review summarizes the current studies that have described signaling mechanism of RIN4 function, modification of RIN4 by bacterial effectors and different interacting partner of RIN4 in defense related pathway. In addition, the emerging role of the RIN4 in plant physiology and intercellular signaling as it presents in exosomes will be discussed.