• Plant Biotechnology Reports
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• v.3 no.3
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• pp.175-182
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• 2009

Simple, reproducible, high frequency, improved plant regeneration protocol in Eastern Cottonwood (Populus deltoides) clones, WIMCO199 and L34, has been reported. Initially, aseptic cultures established from axillary buds of nodal segments from mature plus trees on MS liquid medium supplemented with $0.25mg\;1^{-1}$ KIN and $0.25mg\;1^{-1}$ IAA. Nodal and internodal segments were found to be extra-prolific over shoot apices during course of aseptic culture establishment, while $0.25mg\;1^{-1}$ KIN concentration played a stimulatory role in high frequency plant regeneration. Diverse explants, such as various leaf segments, internodes, and roots from in vitro raised cultures, were employed. Direct plant regeneration was at high frequency of 92% in internodes, 88% in leaf segments, and 43% in root segments. This led to the formation of multiple shoot clusters on established culture media with rapid proliferation rates. Many-fold enhanced shoot elongation and growth of the clusters could be achieved on liquid MS medium supplemented with borosilicate glass beads, which offer physical support for proliferating shoots leading to faster growth in comparison to semi-solid agar or direct liquid medium. SEM examination of initial cultures confirmed direct plant regeneration events without intervening calli. In vitro regenerated plants induced roots on half-strength MS medium with $0.15mg\;1^{-1}$ IAA. Rooted 5- to 6-week-old in vitro regenerated plants were transferred into a transgenic greenhouse in pots containing 1:1 mixture of vermicompost and soil at $27{\pm}2^{\circ}C$ for hardening and acclimatization. 14- to 15-week-old well-established hardened plants were transplanted to the field and grown to maturity. The mature in vitro raised poplar trees exhibited a high survival rate of 85%; 4-year-old healthy trees attained an average height of 8 m and an average trunk diameter of 25 cm and have performed well under field conditions. The regeneration protocol presented here will be very useful for undertaking genetic manipulation, providing a value addition to Eastern Cottonwood propagation in future.

• Clean Technology
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• v.27 no.1
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• pp.24-32
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• 2021

Unburned carbon (UC) was successfully separated from fly ash by up to 85.8% in weight via froth flotation using soybean oil as a collector. An 18 wt% yield of microceramics (CM) could be achieved by employing a hydro cyclone separator located immediately after the flotation equipment. UC and CM were tested as alternatives to weight-adding material and polymer (especially polypropylene in this study) filler, respectively. Large particles of UC were broken down into smaller ones via ball milling to have an average particle diameter of 10.2 ㎛. When crushed UC was used as an alternative to clay as a rubber weight-adding material, a somewhat lower tensile strength and elongation rate than the allowed values were unfortunately obtained. In order to satisfy the standard limits, further treatment of UC is required to enhance surface energy for more intimate bonding with rubber. CM was observed in spherical forms with an average diameter of 5 ㎛. The surface of the CM particles was modified with phenol, polyol, stearic acid, and oleic acid so that the surface modified CM could be used as a polypropylene-filling agent. The flowability was good, but due to the lack of coupling forces with polypropylene, successful impact strength and flexural strength could not be obtained. However, when mixing the surface-modified CM with 1% silane by weight, a drastic increase in both the impact strength and flexural strength were obtained.

• The Korean Journal of Nuclear Medicine Technology
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• v.18 no.2
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• pp.73-77
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• 2014

Purpose Find out about the significance of the GFR values calculated by the kidney depth is measured by comparing the values obtained for kidney depth was measured GFR in the CT image kidney depth and is calculated by Tonnesen law in $^{99m}Tc$-DTPA dynamic kidney scan with each applies. Materials and Methods Among patients with normal value (75~120 mL/min) computed GFR conducted of dynamic renal scan to visit from February 2013 to February 2014 and donor GFR values in patients with normal value. The mean age was 46.9 years with 14 men 13 females. We used abdomen CT image which checked before conducting dynamic Kidney scan for measuring the depth of kidney. We only used CT image that contains renal hilum and measured outermost front of the kidney from the skin surface (a) and the final surface (b) caculated the average depth of [(a + b) / 2] respectively. Using the same ROI in order to limit the change in GFR values by the other additional element was set before and after the depth value was excluded from the GFR falls kidney disease. Results Using Tonnesen law the average value was caculated 5.94 cm from the right kidney 5.90 cm from the left kidney. It was 6.83 cm, 8.71 cm in the left kidney and the right kidney average value of the depth measured on the basis of the CT image. The respective increase in left kidney 0.93 cm and right kidney 2.77 cm calculated on the basis of CT image actually measured values. GFR was calculated as the average depth of the subject calculated by the method Tonnesen $83.3{\pm}9.79mL/min$. $98.6{\pm}14.07mL/min$ GFR was applied to calculate the average depth of the subjects using the CT image, is the difference appears 15.26 mL/min was increased after seting up depth value, P value was less than 0.01 which is significant. Conclusion The difference between GFR before-after setting up depth value cause that the different of depth value. Is a measured depth of the extension value of the calculated estimates Whereas Tonnesen kidney depth method is to use in calculating the value of GFR in a typical dynamic elongation test depth derived using the CT image depth. Is thought to be able to calculate more accurately the GFR value by the distance to the center of kidney more accurately measured in the skin thereby.

• Journal of Sericultural and Entomological Science
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• v.15 no.1
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• pp.37-48
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• 1973

The purpose of this studies is to improve the cohesion of raw silk through various analyses on cocoon drying, cooking, reeling, re-reeling, and on the properties of water. Also we investigated the correlation between silk testing items which we have reached to the following results. 1. Drying of cocoon When cocoons were slowly dried with 100$^{\circ}C$, the results of cohesion became much better. On the other hand, the results were considerably decreased in case the temperature with 115$^{\circ}C$. 2. Cooking of cocoon In case of the cooking of cocoon, we found that the result of cohesion was best with incomplete cooking, that of the control was next, while in over cooking, the results were very low. Also the results of cohesion were much better when using the method of over cooking with sericin arrestive agent than that of incomplete cooking with sericin agglutinating agent. 3. Reeling of cocoon A) When the temperature of reeling bath was 25-45$^{\circ}C$, the results of cohesion test were much better, but at the temperature below 25$^{\circ}C$ or above 45$^{\circ}C$, the results became worse. B) With out the process of croissieur, the results of cohesion were too bad, but in case of croissieur more than 1cm, cohesion became better rapidly. Further more, we understood that the results of cohesion were improving slightly with longer length of croissieur. C) When the velocity of reeling was increased, the results of cohesion also improved. The best results were shown when reeling velocity was 180-220 r.p.m. But when the velocity was increased more than 220 r.p.m., the results of cohesion got worse more or less. D) When the temperature of the drying pipe in reeling machine was raised, the results of cohesion also showed a tendency to improve. 4. Re-reeling A) We could net reach a conclusion as to have correlation between the number of dipping repeat in vacuum tank and the results of cohesion before re-reeling process. B) When we used Seracol 500 as an agglutination protective agent with l/1000 to l/2000 of water, the results of cohesion test were better. C) When we used Pearl-lite as an agglutination protective agent with 1/1000 to 1/2000 of water, the results of cohesion were considerably better. D) We gained tile best results when used Cohesion Improving Chemical, A-80, with 500-1500 times diluted. 1) Results of cohesion was improved when humidity was low or temperature was high in the rereeling machine. 5. Filature water A) The water pH near the isoelectric point of protein showed the best cohesion, but the farther water pH, the worser results were obtained. B) With the increasing of M-alkalinity in filature water, the results of cohesion were worse. Above all, we understood the tendency of the results of cohesion get worse when the M-alkalinity is increased above 200 ppm. C) By increasing the total hardness of the filature water, it improved the results of cohesion. Especially, when the total hardness was above 300ppm, the results were extremely high. 6. Effects combination of each results A) The result of effects combination in filature processes with the obtained best conditions was distinctively improved. But the results could not reach in mathematicaly double effect. When reelied under worse conditions, the results of cohesion test were too bad. There was "effect limit" for the promotion. B) Generally the results of cohesion were bad when the filature conditions(the temperature, pressure and the properties of water, etc) are processed as sericin loss to be high. On the other hand, the results were very good when lower sericin loss was controlled in filature conditions. C) When filature conditions such as reeling velocity and croissieur length provide pysical cohesion ability and when raw silk dry fast during reeling and re-reeling, we found the result of cohesion was better. 7. Correlation of silk testing items. A) A negative correlation exists between the results of cohesion test and cleanness defect. Another word, the result of cohesion test was found to be worse as cleanness defect increased. B) In cleanness, cohesion has negative correlation against the number of slugs, but we could not find any correlation against long loops, loose ends. C) Cohesion has negative correlation against average neatness and low neatness defect. The better the results of neatness respectively, the better the results of cohesion found. D) There is no correlation between tenacity and the results of cohesion test, but there was high positive correlation between the results of elongation and those of cohesion test. The more elongation, the better the results of cohesion was found.

• Research in Plant Disease
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• v.15 no.3
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• pp.187-192
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• 2009

Pepper powdery mildew, Leveillula taurica is one of the most important pathogens of pepper in the greenhouses and fields in Korea and is becoming a worldwide disease. These experiments were carried out to investigate the optimal germination, disease development conditions, disease incidence and effective inoculation methods of pepper powdery mildew. The incidence of powdery mildew was investigated throughout the country based on the major pepper growing areas in 2009. The average rate of infected leaves ranged from 15.3% to 81.8% in greenhouses and fields. Powdery mildew incidences were more severe in greenhouses than those of fields. The optimal temperature for conidial germination was $25^{\circ}C$ and showed the highest germination at 6 hs after treatment. The range of temperature for germination was $10^{\circ}C$ to $35^{\circ}C$. Temperature of greater than $30^{\circ}C$ and below $20^{\circ}C$ affected the abnormal germination and germ tube elongation. The optimal relative humidity for germination and germ tube elongation was 85% and germination increased as relative humidity increased. Disease development started 8 days after inoculation and showed the highest disease severity at 15 days in greenhouse and field regardless of varieties. Among three different inoculation methods tested the spore dropping method was better than touching with infected leaves and spraying with spore suspension. However, the method has limitation in mass inoculation due to the amount of time consumed.

• Journal of Korean Society of Forest Science
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• v.100 no.3
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• pp.432-440
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• 2011

The objective of this study was to investigate growth and fruiting characteristics (e.g., nut qualities) of chestnut (Castanea cerenata) after applying various fertilizer treatments at the cultivation site in Suncheon. Fertilizer treatments were designed as follow: liquid fertilizer, chemical fertilizer, organic fertilizer, and control. Both liquid and chemical fertilizer treatments provided the best growth in height and basal diameter. In addition, these two treatments were very effective for crown width of the trees between both east-west and north-south orientation. The liquid fertilizer treatment was effective on total length of the fruiting branch and length of the bearing to terminate part. Both liquid and chemical fertilizer treatments produced the longest length of basal to bearing part compared to the other two treatments. The liquid fertilizer treatment showed the most thickened basal diameter of the fruiting branch and the greatest diameter of above and below the bearing burr part. Elongation Index of the fruiting branch (EI) was the highest with liquid fertilizer treatment and the remaining four indices (Production Index of fruiting branch, PI; Ratio of Diameter between below and above bearing burr part, RD; Growth Index of fruiting branch diameter, GI; Thickness Index of fruiting branch or dormant branch, TI) were the highest with the chemical fertilizer treatment. Total number of produced branch per fruiting mother branch and number of small and weak branches per fruiting mother branch were highest on the control and liquid fertilizer treatment; however, all treatments produced similar numbers. The chemical and organic fertilizer treatments produced a high number of fruiting branches per fruiting mother branch, while organic and liquid fertilizer treatments produced a high number of burr per fruiting branch. The rate of commercializing on the basis of nut weight and quantity was higher on control (87.5%) than chemical fertilizer treatment (84.6%); however, the rate was even lower on liquid fertilizer treatment (84.3%) and organic fertilizer treatment (82.7%). The liquid fertilizer treatment showed the highest average of nut weight, while chemical fertilizer treatment showed the highest average number of fruiting burr. There was no significant difference in average number of normal nuts per burr among treatments. The yield per tree was high on chemical (8.2 kg) and liquid (8.0 kg) fertilizer treatments, but there was no significant difference among treatments. In the rate of nut grade on the basis of nut weight and quantity, the liquid fertilizer treatment, 43.5% and 34.3% more than large nut respectively, produced higher value chestnuts compared to other treatments.

• Journal of Life Science
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• v.25 no.9
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• pp.1007-1013
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• 2015

A COG (Cluster of Orthologous Groups of proteins) algorithm was applied to detect conserved genes in 711 prokaryotes. Only COG0080 (ribosomal protein L11) was common among all the 711 prokaryotes analyzed and 58 COGs were common in more than 700 prokaryotes. Nine COGs among 58, including COG0197 (endonuclease III) and COG0088 (ribosomal protein L4), were conserved in a form of one gene per one organism. COG0008 represented 1356 genes in 709 of the prokaryotes and this was the highest number of genes among 58 COGs. Twenty-two COGs were conserved in more than 708 prokaryotes. Of these, two were transcription related, four were tRNA synthetases, eight were large ribosomal subunits, seven were small ribosomal subunits, and one was translation elongation factor. Among 58 conserved COGs in more than 700 prokaryotes, 50 (86.2%) were translation related, and four (6.9%) were transcription related, pointing to the importance of protein-synthesis in prokaryotes. Among these 58 COGs, the most conserved COG was COG0060 (isoleucyl tRNA synthetase), and the least conserved was COG0143 (methionyl tRNA synthetase). Archaea and eubacteria were discriminated in the genomic analysis by the average distance and variation in distance of common COGs. The identification of these conserved genes could be useful in basic and applied research, such as antibiotic development and cancer therapeutics.

• Journal of Korea Foresty Energy
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• v.25 no.2
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• pp.9-15
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• 2006

The quality of wood is largely depend on the characteristics of xylem tissue and their variation. They may include tracheid length, microfibril angle, distribution and amount of resin canal, as well as specific gravity as indicator of material properties. In this study, variation of these elements between and within 30 superior families of Pinus densifloa in progeny test forest were examined and the results are as follows; In terms of elongation ratio of tracheid length which is less than 1%, the sample tree showed a transition to matured wood after 13 years particularly in Kangwon 25, 30, 90, 56 and Chungnam 4 families. The average specific gravity were from 0.35 to 0.49, and differences between the families were significant. Number of transverse resin canal per unit area were also found to be significant between the families. The microfibril angles measured at 15th ring number ranged from 0.9 degree to 28.6 degree. More studies are necessary to tell whether these variation is inherited by genetic or individual characteristic. However it would be desirable that these elements be considered as key elements in the early stage of selection process of superior tree to ensure good quality of wood production in future.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.1
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• pp.31-44
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• 1989

In the vinyl house cultivation, the water consumption, evapotnanspirafion ratio, growth condifion by the growing stages and yields of tomato and cucumber were investigated when they cultivated by nutriculture, rice hull charcoal culture and NFF culture. The results obtained are summarized as follows: 1. The mean air temperature in the vinyl house during the experimental perica j was 1.9$^{\circ}$C higher than the outdoor air temperature, the rrnocirnum and minimum air temperature in the vinyl house was 4.6C and 1.7$^{\circ}$C higher than the outdoor temperature, respectively, and the mean daily evaporation in the vinyl house during the experimental period was 4.3rnm that is 02rnrn more than the outdoor evaporation 2. In the tomato cultivation, the growth of rice hull charcoal culture was the best among the three methods, and the growth of NFT culture and nutriculture were worse than the soil cultivation which is standard, and among the nutriculture, the growth of EC 1.3m.mho plot was the best. 3. In the cucumber cultivation, the growth of rice hull charcoal culture was the best, and while the growth condition of NFT culture was worse than the standard soil cultivation, the growth of nutriculture was better than the standard soil cultivation, and the growth of EC 1.3m.mho plot was the highest among the different treatments in the nutriculture. 4. In the tomato cultivation, the total water consumption of rice hull charcoal culture plot was 1107.5rnm which is the highest amount among the three methods, and in the nutriculture, the total water consumption of EC 1.3m.mho plot was 22lAmm which is the highest among the three different treatments. 5. In the cucumber cultivation, the total water consumption of rice hull charcoal culture was 11762rnm which is the highest, and the total water consumption of EC 1.3m.mho plot was 284.9rnm which is more than the 278.9mm of EC 1.Smmho plot and 262.9mm of EC 1.7rnmho plot. 6. The crop coefficient(Kc) of tomato was 0.82 in NFT culture, 4.67 in rice hull charcoar culture and 0.86~0.91 in nutriculture. 7. The crop coefficient(Kc) of cucumber was higher than tomato as 1.13 in NFT culture, 520 in rice hull charcoal culture and 1.08~1.19 in nutriculture. 8. The evapotranspiration ratio in the mid and late season were higher than the beginning and elongation stage, and the average evaportranspiration ratio of tomato and cucumber was 3.81 and 424, respectively, in the rice hull charcoal culture plot. 9. In the tomato cultivation, the total yield per plant of rice bull charcoal culture was 1443.Og which is the highest, and in the nutriculture, their yields were worse because of the damage of downy nidew disease. 10. In the cucumber cultivation, the total yield per plant of rice hull charcoal culture was 1965.7g which is the highest, and while the yield of NFT culture was ahout 25% lower than the stadard soil cultivation, the yield of nutriculture was higher than the standard soil cultivation, and among the treatments in the nutriculture, the yield of EC 1.3m.mho plot was the highest.

• Progress in Medical Physics
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• v.25 no.4
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• pp.205-209
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• 2014

Medical polymers require sterilization and must be able to maintain material properties for a specified shelf life. Sterilization can be achieved by using gamma or e-beam exposure. In this study, accelerated aging tests of poly(ether-block-amide) (PEBA) copolymer samples is presented. PEBA copolymer samples with different polyether content that result in Shore hardness of 35D to 72D, were sterilized using e-beam radiation followed by accelerated aging at $55^{\circ}C$. E-beam sterilization effect on molecular weight and mechanical property has performed and analyzed. The average molecular weight significantly reduced as a result of ageing. The enlarged proportion of low molecular weight chains in the aged samples is consistent with the generation of degradation products produced by oxidative chain scission. Also E-beam materials have shown decreased tensile strength and elongation. Overall, this study demonstrated that the medical grade PEBA was significantly affected by radiation exposure over aging time, particularly at high irradiation doses. For medical use in case of radiation sterilization required, it is recommended to avoid Pebax material. If Pebax material must be in use for medical device, recommend to use alternate sterilization method such as Ethylene Oxide sterilization.