• Journal of Internet Computing and Services
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• v.7 no.2
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• pp.81-92
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• 2006

Recently, the number of mobile phone and PDA users has been rapidly increased. Such monitoring and control applications as geographical and traffic information systems are being used widely with wireless devices. In this paper, we introduce the mobile M/VC application framework that supports the rapid constructions of mobile monitoring and control (M/VC) applications. The mobile M/VC application framework uses the mobile Observer/Observable pattern that extends the Java's Observer/Observable for automatic interactions of server and client objects in wireless environments. It also provides the Multiplexer and Demultiplexer classes that supports the assembly feature of Observer and Observable objects. To construct an application using the framework, developers just need to create necessary objects from the Observable and MobileObserver classes and inter-connect them structurally(like the plug-and-play style) through the Multiplexer and Demultiplexer objects. Then, the state change of Observable objects is notified to the connected Observer objects and user's input with Observer objects is propagated to Observable objects. These mechanism is the main process for monitoring and control applications. Therefore, the mobile M/VC application framework can improve the productivity of mobile applications and enhance the reusability of such components as Observer and Observable objects in wireless environments.

• Molecules and Cells
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• v.41 no.12
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• pp.1016-1023
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• 2018

Regenerative orthopedics needs significant devices to transplant human stem cells into damaged tissue and encourage automatic growth into replacements suitable for the human skeleton. Soft biomaterials have similarities in mechanical, structural and architectural properties to natural extracellular matrix (ECM), but often lack essential ECM molecules and signals. Here we engineer mineralized polysaccharide beads to transform MSCs into osteogenic cells and osteoid tissue for transplantation. Bone morphogenic proteins (BMP-2) and indispensable ECM proteins both directed differentiation inside alginate beads. Laminin and collagen IV basement membrane matrix proteins fixed and organized MSCs onto the alginate matrix, and BMP-2 drove differentiation, osteoid tissue self-assembly, and small-scale mineralization. Augmentation of alginate is necessary, and we showed that a few rationally selected small proteins from the basement membrane (BM) compartment of the ECM were sufficient to up-regulate cell expression of Runx-2 and osteocalcin for osteoid formation, resulting in Alizarin red-positive mineral nodules. More significantly, nested BMP-2 and BM beads added to a non-union skull defect, self-generated osteoid expressing osteopontin (OPN) and osteocalcin (OCN) in a chain along the defect, at only four weeks, establishing a framework for complete regeneration expected in 6 and 12 weeks. Alginate beads are beneficial surgical devices for transplanting therapeutic cells in programmed (by the ECM components and alginate-chitosan properties) reaction environments ideal for promoting bone tissue.