• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.10a
• /
• pp.13-13
• /
• 2019

Soybean (Glycine max L. (Merr) is commonly consumed and found in major foods including soymilk, soy sauce, tofu, and soy sprout in Korea and east Asia. In addition, it is common to cook the whole seeds with rice. Soybean is known to have ranges of health benefits including antiaging, anticancer, neuroprotective and antidiabetic taken either as supplement or dietary food. Anthocyanins and flavonoids in G. max are found to be the main contributors to such wide arrays of health benefits. Due to increasing economic values of soybean, development of specialty soybean cultivars is becoming an area of interest worldwide. In this study, 746 black soybean accessions from National Agrobiodiversity Center were characterized as part of an attempt to identify important germplasms of G. max. Seed coats of each accession were analyzed for their total anthocyanin, cyanidin 3-O-Glucoside (C-3-O-G), delphinidin 3-O-glucoside (D-3-O-G), petunidin-3-O-glucoside (Pt-3-O-G), and their whole seeds for crude protein contents. HPLC was used to determine and quantify the anthocyanin compositions while crude protein was determined using Kjeldahl method by Kjeltec auto-analyzer (Kjeltec 8400, Foss, Sweden). Accessions were grouped according to their anthocyanins and protein contents; the mean content of which were correlated to agronomic traits including maturity date, one hundred seed weight, cotyledon color and seed lust color. The results indicated that the total anthocyanin content (TAC) ranged from 273.77 to 6250.52 mg/100 g, with mean value of 1853.03 mg/100 g while the crude protein content (CPC) being between 33.43 and 47.51%, with mean value of 40.81%. The highest number of accessions (45.97%) showed TAC between 1000~1900 mg/100 g while 30.96% of accessions showed CPC between 41~43%. Among the 746 accessions considered, 11 (IT142935, 175818, 175855, 177191, 177209, 177211, 177214, 177216, 177218, 177220, 177274) of them showed TAC above 4000 mg/100 g. C-3-O-G was found to be the major contributor to TAC showing strong correlation. Accessions with green cotyledon color showed high mean TAC compared to those having yellow cotyledon color, and accessions with dull seed lust color showed high mean TAC than those having shiny seed lust color. One hundred seeds weight and maturity date showed positive correlation with all anthocyanin contents, except for Pt-3-O-G in the latter case. The overall result of the present study could be used as background for developing new black soybean cultivars and breeds with high anthocyanin and protein contents. The result depicted that many of the accessions could be used as potential parental lines.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.18 no.1
• /
• pp.153-157
• /
• 2014

Purpose: SLE (systemic lupus erythematosus) is an inflammatory autoimmune disease, characterized by various autoantibody. The detection of Anti double-stranded DNA (Anti-ds DNA) is important in the diagnostics of SLE, and include the American College of Rheumatology (ACR) diagnostic criteria for SLE. Also SLE disease activity and correlativity with the level Anti-ds DNA antibody have been reported and Anti-ds DNA antibody quantitative test is very useful for tracing before and after SLE treatment. When These Anti-ds DNA antibody test (Farr assay: $^{125}I$ labeled ds-DNA and bound Anti-ds DNA antibodies complex in serum is precipitated by ammonium sulfate and used to centrifugation, measured it) inhaled supernatant after centrifugation, a lipemic specimen does not facilitate the formation of precipitate and also occurs situation was inhaled with precipitate. To solve these problems, The Influence of the degree of lipemic specimen was evaluated. Materials and Methods: September 2012 to February 2013, We selected lipemic samples (n=81) of specimen commissioned by Anti-ds DNA antibody test. Lipemic samples were done pre-treatment (high-speed centrifugation: 14,000 rpm 5 mins) used a micro-centrifuge (Eppendorf Model 5415D). At the same time lipemic specimen and pre-treatment samples were performed Anti-ds DNA antibody test (Anti-ds DNA kit, Trinity Biotech, Ireland). Statistical analysis were analyzed Pearson's correlation coefficients and regression and paired t-test, and Difference (%). Results: Experimental group 1 (Lipemic Specimen Anti-ds DNA Ab concentration ${\leq}7IU/mL$) at y=0.368X+4.732, $R^2=0.023$, Pearson's correlation coefficient was 0.154, paired t-test (P=0.003), Difference (%) mean 65.7 and showed a statistically significant difference. Experimental group 2 (Lipemic Specimen Anti-ds DNA Ab concentration ${\geq}8IU/mL$) at y=0.983X+0.298, $R^2=0.994$, Pearson's correlation coefficient showed 0.997, paired t-test (P=0.181), Difference (%) mean -5.53 made no statistically significant difference. Conclusion: Lipemic sample of low Anti-ds DNA Ab concentration (2.5-7 IU/mL) and the result is obtained pre-treatment (high-speed centrifugation: 14,000 rpm 5 mins) were made a significant difference statistically. Anti-ds DNA is one of the primary auto-antibodies present in patients with SLE, and remain an important diagnostic test for SLE. Therefore, we recommend preprocessing (high-speed centrifugation: 14,000 rpm 5 mins) in order to exclude the influence of lipemic specimen.

• Restorative Dentistry and Endodontics
• /
• v.30 no.2
• /
• pp.112-120
• /
• 2005

This study was done to evaluate whether there were any differences in microleakage of class V composite restorations according to restoration site and cavity size. Total sixty-four restorations were made in molar teeth using Esthet-X. Small ($2\;{\times}\;2\;{\times}\;1.5\;mm$) and large ($4{\times}2{\times}1.5\;mm$) restorations were made at the buccal/lingual surface and the proximal surface each. After 1,000 times of thermocycling ($5^{\circ}\;-\;55^{\circ}C$), resin replica was made and the percentage of marginal gap to the whole periphery of the restoration was estimated from SEM evaluation. Thermocycled tooth was dye penetrated with $50\%$ silver nitrate solution. After imbedding in an auto-curing resin, it was serially ground with a thickness of 0.25 mm. Volumetric microleakage was estimated after reconstructing three dimensionally. Two-way ANOVA and independent T-test for dye volume, Mann-Whitney U test for the percentage of marginal gap, Spearman's rho test for the relationship between two techniques were used, The results were as follows : 1. The site and size of the restoration affected on the microleakage of restoration. Namely, much more leakage was seen in the proximal and the large restorations rather than the buccal/lingual and the small restorations. 2. Close relationship was found between two techniques (Correlation coefficient = 0.614/ P = 0.000). Within the limits of this study, it was noted that proximal and the large restorations leaked more than buccal/lingual and the small restorations. Therefore, it should be strictly recommended large exposure of margins should be avoided by reducing unnecessary tooth reduction.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.33 no.6
• /
• pp.2447-2453
• /
• 2013

In this study, the effects of weather conditions such as rainfall, discomfort index, snowfall, and sensible temperature on public transport demand in Seoul were analyzed using statistical data. The reasons were also derived from the survey. The data for the analysis were collected over the weekdays and weekends, and seasonal data of summer and winter were also gathered separately. Rainfall amount, discomfort index, and sensible temperature except snowfall amount, whose samples were insufficient, decreased the public transport demand by 2-7%. Rainfall amount and sensible temperature were statistically significant. Correlation analysis also showed that rainfall amount and sensible temperature are highly correlated with the demand. To find the reasons, the survey was conducted on citizens living in the Seoul Metropolitan Area. About 30% of the respondents wished to give up using bus when rainfall was heavy or temperature was low. On the contrary, auto and subway users increased by 10%. The results of this study could be used as the basic data when the public transportation planning or operation related policies according to the weather condition are concerned.

• Journal of Korean Society of Forest Science
• /
• v.105 no.1
• /
• pp.122-131
• /
• 2016

This study was conducted to analyze the effect of climatic variables on tree-ring growth of Larix leptolepis distributed in Korea by dendroclimatological method. For this, annual tree-ring growth data of Larix leptolepis collected by the $5^{th}$ National Forest Inventory were first organized to analyze yearly growth patterns of the species. To explain the relationship between tree-ring growth of Larix leptolepis and climatic variables, monthly temperature and precipitation data from 1950 to 2010 were compared with tree-ring growth data for each county. When tree-ring growth data were analyzed through cluster analysis based on similarity of climatic conditions, six clusters were identified. In addition, index chronology of Larix leptolepis for each cluster was produced through cross-dating and standardization procedures. The adequacy of index chronologies was tested using basic statistics such as mean sensitivity, auto correlation, signal to noise ratio, and expressed population signal of annual tree-ring growth. Response function analysis was finally conducted to reveal the relationship between tree-ring growth and climatic variables for each cluster. The results of this study are expected to provide valuable information necessary for estimating local growth characteristics of Larix leptolepis and for predicting changes in tree growth patterns caused by climate change.

• Journal of the Korean earth science society
• /
• v.31 no.2
• /
• pp.119-128
• /
• 2010

To make reliable interpretations on the sparse spatial data, the spatial distribution characteristics that are inevitable for spatial estimation should be properly analyzed. Variograms have been widely used for obtaining the spatial characteristics inherent to data in spatial estimation problems. But their applications were limited as the basic information for further data estimation. Therefore, the additional analysis of the meaning of variograms is required for more reliable data processing and interpretations. In this paper, we investigated the proper meaning of variogram values and the specific features of distributions which can be obtained through variogram analysis. Variograms can provide the information on both linearity and the strength changes of interrelationships between the data sets according to the direction and lag distance. First, sill and range values, which are main parameters of variograms, were analyzed. Then a similarity range using spatial auto-correlation values was introduced to verify the applicability of linearity analysis through the comparative study of spatial distribution features of gravity and magnetic data collected in Hwasan caldera. Through these analyses, we were able to identify the dissimilar patterns of gravity and magnetic data that became apparent according to the distribution and variation ranges of the data sets. It is inferred that the gravity and magnetic anomalous bodies are extended to the ground because linearity direction of gravity and magnetic data appear similarly with linearity derection of topography in Hwasan caldera.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.17 no.2
• /
• pp.93-101
• /
• 2015

This study was conducted to analyze the relationship between tree-ring growth of Quercus acutissima and climatic variables by dendroclimatological method. Annual tree-ring growth data of Quercus acutissima collected by the $5^{th}$ National Forest Inventory (NFI5) were organized to analyze the spatial distribution of the species growth pattern. To explain the relationship between tree-ring growth of Quercus acutissima and climatic variables, monthly temperature and precipitation data from 1950 to 2010 were compared with tree-ring growth data for each county. When tree-ring growth data were analyzed through cluster analysis based on similarity of climatic conditions, four clusters were identified. In addition, index chronology of Quercus acutissima for each cluster was produced through cross-dating and standardization procedures. The adequacy of index chronologies was tested using basic statistics such as mean sensitivity, auto correlation, signal to noise ratio, and expressed population signal of annual tree-ring growth. Response function analysis was conducted to reveal the relationship between tree-ring growth and climatic variables for each cluster. The results of this study are expected to provide valuable information necessary for estimating local growth characteristics of Quercus acutissima and for predicting changes in tree growth patterns caused by climate change.

• Journal of Korean Society of Environmental Engineers
• /
• v.38 no.8
• /
• pp.444-451
• /
• 2016

Most research on membrane fouling models in the past are based on theoretical equations in lab-scale experiments. But these studies are barely suitable for applying on the full-scale spot where there is a sequential process such as filtration, backwash and drain. This study was conducted in submerged membrane system which being on operation auto sequentially and treating wastewater from G-water purification plant in Incheon. TMP had been designated as a fouling indicator in constant flux conditions. Total volume of inflow and SS concentration are independent variables as major operation parameters and time-series analysis and prediction of TMP were conducted. And similarity between simulated values and measured values was assessed. Final prediction model by using genetic algorithm was fully adaptable because simulated values expressed pulse-shape periodicity and increasing trend according to time at the same time. As results of twice validation, correlation coefficients between simulated and measured data were $r^2=0.721$, $r^2=0.928$, respectively. Although this study was conducted limited to data for summer season, the more amount of data, better reliability for prediction model can be obtained. If simulator for short range forecast can be developed and applied, TMP prediction technique will be a great help to energy efficient operation.

• Journal of Korean Society of Forest Science
• /
• v.99 no.6
• /
• pp.908-913
• /
• 2010

This study analyzed the impact of climatic and topographic factors on tree radial growth of Pinus densiflora and Quercus spp. in central regions of Korea. To find the relationship between annual tree radial growth and climatic factors, we took the core samples from individual trees and measured the tree radial width. On the assumption that the tree radial growth is related to the tree age, we estimated the radial growth by the tree age as an independent variable. Also, we estimated the standard growth, defined as the radial growth of trees aged 30. As results, we found the spatial auto-correlation in the radial growth of the red pine. Moreover, we also found the relationships between climatic and topographic and the standard growth using the GAM (Generalized Additive Model). Increase of temperature has negative impacts on the radial growth of Pinus densiflora, while it has positive impacts on the radial growth of Quercus spp.. On the other hands, increase of precipitation has negative impacts on the radial growth of both species. Lastly, we predicted the spatial distribution changes of Pinus densiflora and Quercus spp. using the temperature increase scenario and the Geographic Information System (GIS) based forest type map. We could predict that Pinus densiflora is more vulnerable than Quercus spp. to climate change so that the habitats of Pinus densiflora will be gradually changed to the habitats of Quercus spp. in eastern coastal and southern regions of Korea after 60 years.

• Journal of Nutrition and Health
• /
• v.41 no.8
• /
• pp.767-775
• /
• 2008

Uncoupling protein-1 (UCP-1) plays a major role in thermogenesis at brown adipose tissues and has been implicated in the pathogenesis of obesity and metabolic disorders. The purpose of this study was to estimate the effects of A-3826G polymorphism in 117 Korean prepubertal children aged 8-11 years olds. Anthropometry by bioelectrical impedance analysis method, plasma lipid profiles by auto-biochemical analyzer and UCP-1 genotyping by PCR-RFLP were done. The frequencies of UCP-1 genotypes were AA; 17.7%, AG; 57.8%, GG; 26.6%. The frequencies of each G allele (55.5%) was similar to Japanese's (49%) and higher than Caucacian's (25%). No correlation UCP-1 polymorphism and BMI (kg/$m^2$) or the degree of obesity described by the relative percentiles of the standard weight according to height in prepubertal children. However, plasma total- and LDL-cholesterol were significantly increased in G allele when sex, age and weight were adjusted. Our results suggested that G allele of UCP-1 gene was stronger risk factors in hyperLDLcholesterolemia than A allele. This impact might be progressed as the precaution against the revalence of obesity based-metabolic disease.