• The Korean Journal of Mycology
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• v.18 no.1
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• pp.20-25
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• 1990

For the purpose of obtaining microorganisms producing high fructosyl transferase, the screening test was carried out. Among more than three hundred isolates, an isolate (C23-isolate) was selected for high fructosyl transferase producer from the dirts at the coffee vendig machine. The morphological and cultural characteristics of the isolate C23 on various culture media were studied and identified as Aureobasidium pullulans var. melanigenum.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.417-422
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• 1990

For optimal production of fructosyl transferase in AureobasidiumpuZZulane C-23, the effect of fermentation conditions for cell growth and fructosyl transferase production were investigated. Sucrose was excellent carbon source. Sucrose concentration for the optimum production of fructosyl transferase was 35%. Enzyme productivity was significantly increased by addition of ammonium oxalate and yeast extract. A time course study for the enzyme production by Aureobasidium pullutans C-23 was carried out. At 2 days incubation, the production of intracellular enzyme was maximum. The extracellular enzyme production was found to be increased up to 6 days.