• 대한본초학회지
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• 제22권4호
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• pp.127-135
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• 2007

Objectives : In this study we investigated the antiobese effects of mixed pills of pickled black soybeans with herbs(herbal Chokong pill, hereafter HCKP) in rats fed high-fat diet. It was evaluated by measuring the changes of body weight, adipose tissues weight and lipid profiles in serum. Methods : Black soybeans were pickled in vinegar for 15 days to prepare Chokong, at room temperature. For treatment group, HCKP was prepared, which five kinds of medicinal herbs(Rhynchosia nulubilis, Glycyrrhiza uralensis, Zizyphus vulgaris, Atractylodes macrocephala K, Astragalus membranaceus and Cornus officinalis) were added to dried Chokong. Four groups of male Sprague-Dawley rats were fed different diets during 9 weeks: normal diet containing 5%(w/w) com oil, high-fat diet containing 10%(w/w) lard plus 5%(w/w) corn oil (HF), high-fat diet supplemented with 1%(T1) and 5%(T5) HCKP powder, respectively. Results : The T5 group had markedly lower body weight gain and weights of epididymal adipose tissue when compared with HF group. There were significant differences in visceral adipose tissue weights, serum total cholesterol and triglyceride concentrations between the HF and T5 group. Then, the efficacy of powered HCKP on body weight and lipid profiles change in rats fed high-fat diet were induced dose dependantly. Conclusion : These results suggest that the possibility of HCKP, as an antiobese functional formula, by suppression of body weight gain and improved lipid profiles.

• 한국약용작물학회지
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• 제25권1호
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• pp.1-9
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• 2017

Background: Some plants have harmful effects on fungi and bacteria as well as other plants. Incorporating such plant into soil as green manure is effective in reducing population densities of soil pathogens. Methods and Results: Twenty-three species of green manure crops were cultivated after the harvest of 6-year-old ginseng and then incorporated into the soil at the flowering stage. The following year, the root rot ratio of 2-year-old ginseng and soil chemical properties were investigated. In the absence of green manure addition, the $NO_3$ content, electric conductivity (EC), and K content decreased by 95%, 79% and 65%, respectively. In the presence of green manure addition, $P_2O_5$ and $NO_3$ contents reduced by 41% and 25%, respectively. The "survived root ratio" of 2-year-old ginseng significantly increased by 56.2%, 47.5%, and 47.3%, in the Sorghum sudanense, Ricinus communis and Helianthus tuberosus treatment, respectively. In addition, there was a significant increase in the "survived root ratio" in the Secale cereale, Chrysanthemum morifolium, Atractylodes macrocephala, and Smallanthus sonchifolius treatments. The "survived root ratio" of ginseng showed a significant positive correlation with the soil pH and a negative correlation with the $NO_3$ contents, and EC. Conclusions: Cultivation of plant form the Chrysanthemum family as green manure, using mainly the rhizomes was effective for the control of root rot disease of ginseng.

• 한방재활의학과학회지
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• 제27권4호
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• pp.85-96
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• 2017

Objectives To summarize and evaluate the efficacy of traditional Chinese herbal medicine (TCHM) treatment for antibiotic associated diarrhea (AAD). Methods Eight electronic databases were searched from their inception to August 2017. Randomized controlled trials (RCTs) assessing the efficacy of TCHM treatment for AAD were included. The risk of bias was assessed using the Cochrane risk of bias assessment tool. Data analysis was performed using RevMan software version 5.3. Results Seventeen RCTs involving 1138 patients with AAD were included for qualitative synthesis. TCHM treatment improved total effective rate (TER). However, the results that TER in experimental group was significantly higher than in control group were different between the included studies. TCHM enema treatment improved TER, but not significantly higher than control group. The most frequently used herbal formulas were Gamiwekwanjeon, Gamiinsampaedoksan, and Samryungbaekchulsan. The most frequently used TCHMs were Atractylodes macrocephala (Bai Zhu), Dioscorea batatas (Shan Yao). Within the studies documenting the adverse events, no serious adverse events associated with TCHM treatment were observed. Conclusions Evidence of TCHM treatment efficacy for AAD is encouraging, but not conclusive, because of the low methodological qualities, diversity of TCHM treatment prescriptions. Further well-designed RCTs with rigorous randomization and blinding method are needed to confirm these results.

• 동의신경정신과학회지
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• 제11권1호
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• pp.115-130
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• 2000

Object: Show the treatment about Jungkijeung (Zhongqizheng) by distinguishing Apoplexy(Zhongfeng), yujungfung(Leizhongfeng), gualjung(Juezheng), and observation of cause, process, quality. Method: Researched definition, cause, process, treatment and herb med through chinese & korean medical publication refered to Jungkijeung(Zhongqizheng) Result: 1. Jungkijeung(Zhongqizheng) is simmilar to Apoplexy(Zhongfeng) that spiritual shock occurred to syncope, dysarthria, trismus, quadriplegia. But the symptom of Jungkijeung(Zhongqizheng) is coldness, no-sputum, sink-pluse; that of Apoplexy(Zhongfeng) is warmness, much secretion, float-pulse. 2. Jungkijeung(Zhongqizheng) is mainly caused by the serious anger and the reverse movement of spints by the seven emotional factor. The process of Jungkijeung(Zhongqizheng) are "Fire and Fever(huore)" "Weatness and sputum(shitan)", and most importantly "Weakness of vital-qi(qixi)" 3. The treatment of Jungkijeung(Zhongqizheng) is adjustment and circulation of jiao. In early stage, don't use of Apoplexial- Med. 4. As following herb-med are used for Jungkijeung (Zhongqizheng). Sohaphang-won(Suhexiangyan) is 18 times; Palmisungi-san(Baweishunqisan) is 13 times; Kang-tang(Jiangtang) is 8 times: Mokhyangsungi san(Muxiangshunqisan) is 6 times. 5. Atractylodes macrocephala KOIDZ(Baishu) is used for 40 times most frequently, Saussurea lappa CLARKE (Muxiang), Cyperus rotundus L (Xiangfuzi), Citrus unshiu MARCOR(Chenpi), Glycyrrhiza uralensis FISCH (Gancao), poria cocos WOLF (Furing), Panax ginseng NESS (Renshen) etc are orderly used. 6. Acupoints same as GV20(Baihui), LI4(Hegu), Liv2(Xingjian), 12 Junghyul(Jingxue) is used for acupunture. And CV8(Shenque), CV4(Guanyan) is used for moxibustion. Conclusion: As Jungkijeung(Zhangqizheng) is differed from Apoplexy(Zhongfeng), yujungfung(Leizhongfeng), gualjung(Juezheng), we must also cure Jungkijeung (Zhongqizheng) to the other disease. It is need to distinguishment Jungkijeung(Zhongqizheng) from neurotic, psychotic disease, though similar to conversation neurosis.

• 대한한의학회지
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• 제31권2호
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• pp.149-157
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• 2010

Objective: The purpose of this study was to investigate the level of safety on liver functions when Korean herbal medicine was taken internally. Method: 101 inpatients who took Korean herbal medicine were enrolled and liver function test (aspartic aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, gamma glutamyltranspeptidase, albumin, lactate dehydrogenase) was performed on admission and 1 month later. Results: In 101 patients, alkaline phosphatase and gamma glutamyltranspeptidase decreased significantly compared with the value taken on admission (p<0.05) but aspartic aminotransferase, alanine aminotransferase, total bilirubin, albumin, and lactate dehydrogenase were not significantly changed (p>0.05). In the patients who took Scutellaria baicalensis (n=34), alkaline phosphatase decreased and albumin increased significantly (p<0.05). Among the patients who took Atractylodes macrocephala (n=29), alkaline phosphatase decreased significantly (p<0.05). In the patients who took Glycyrrhiza uralensis or Paeonia lactiflora, liver function parameters were not significantly changed (p>0.05). On admission 11 patients had abnormal liver function and 2 patients had liver injury while 7 patients had abnormal liver function and 2 patients showed liver injury 1 month later. Conclusions: This study suggests that prescribed Korean herbal medicine does not injure liver function.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.87-87
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• 2019

The consumption of herbal medicine and related products (herbal products) have increased in South Korea. At the same time the quality, safety, and efficacy of herbal products is being raised. Currently, the herbal products are standardized and controlled according to the requirements of the Korean Pharmacopoeia, the National Institute of Health and the Ministry of Public Health and Social Affairs. The validation of herbal products and their medicinal component is important, since many of these herbal products are composed of two or more medicinal plants. However, there are no tools to support the validation process. Interest in deep learning has exploded over the past decade, for herbal medicine using algorithms to achieve herb recognition, symptom related target prediction, and drug repositioning have been reported. In this study, individual images of four herbs (Panax ginseng C.A. Meyer, Atractylodes macrocephala Koidz, Poria cocos Wolf, Glycyrrhiza uralensis Fischer), actually sold in the market, were achieved. Certain image preprocessing steps such as noise reduction and resize were formatted. After the features are optimized, we applied GoogLeNet_Inception v4 model for herb image recognition. Experimental results show that our method achieved test accuracy of 95%. However, there are two limitations in the current study. Firstly, due to the relatively small data collection (100 images), the training loss is much lower than validation loss which possess overfitting problem. Secondly, herbal products are mostly in a mixture, the applied method cannot be reliable to detect a single herb from a mixture. Thus, further large data collection and improved object detection is needed for better classification.

• The Plant Pathology Journal
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• 제37권2호
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• pp.162-172
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• 2021

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross-family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and amino acid sequence identities of 97.03% and 98.50%, respectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue. This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

• 동국한의학연구소논문집
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• 제6권1호
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• pp.205-229
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• 1997

금원사대가(金元四大家) 중의 한 사람인 주진형(朱震亨)은 주화론(主火論)의 영향을 받았지만 독창적인 "상화론(相火論)"과 "음부족이양유여(陰不足而陽有餘)"하다는 견해를 밝히면서 보음정이억상화(補陰精而抑相火)를 위주로한 치법(治法)을 제창하여 후대(後代)에 "자음파(滋陰派)"라고 불리운다. 이러한 단계(丹溪)의 의학사상(醫學思想)과 한방부인과학(韓方婦人科學)과 관련된 이론을 개괄하여 단계(丹溪)의 이론과 처방이 우리나라 한방(韓方) 부인과학(婦人科學)의 발전과정에 미친 영향을 고찰하였다. 그 결과 첫째, 단계(丹溪)의 증치사상(證治思想)의 특징은 거습담(祛濕痰), 조기(調氣), 자음청열(滋陰淸熱)의 치법(治法) 운용(運用)과 금욕적(禁慾的) 섭생(攝生)을 통한 양생론(養生論)이었으며, 둘째로 단계(丹溪)의 이론(理論)과 처방(處方)들은 <의방류취(醫方類聚)>와 <동의보감(東醫寶鑑)>에서 <단계심법(丹溪心法)>과 <단계심법부여(丹溪心法附餘)>의 내용을 대폭 수용함으로써 우리나라 한방부인과학(韓方婦人科學) 이론(理論)의 주요한 부분을 형성하는 계기가 되었으며, 셋째로, 부인과질환과 관련하여 월경병(月經病)에 조기(調氣) 및 거습담(祛濕痰)을, 불임증(不姙症)에는 월경불조(月經不調)와의 관련성(關聯性)은 물론 체형(體型) 체질별(體質別) 병기(病機)와 치법(治法)을 구분하였으며, "산전당청열양혈(産前當淸熱養血)" 이론에 의한 황금(黃芩) 백출(白朮)을 위주로 한 안태론(安胎論), "종담론치(從痰論治)"한 악조치법(惡阻治法), 난산예방(難産豫防), 산후(産後)의 "대보기혈(大補氣血)"등의 이론을 제시하여 단계(丹溪)의 학술사상이 우리나라 한방부인과학에 미친 영향은 많았던 것으로 사료된다.

• 생명과학회지
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• 제18권2호
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• pp.200-205
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• 2008

본 연구는 한방초콩환의 고지방식이를 섭식한 흰쥐의 간조직 지방제거 효과를 관찰하기 위하여 서목태(Rhynchosia nulubilis)를 식초에 15일간 절인 후, 5종류의 한약재(감초, 산조인, 백출, 황기, 산수유)를 첨가하여 한방초콩환을 제조하고, 고지방식이로 흰쥐의 비만을 유도하면서 고지방식이에 한방초콩환을 1%와 5% 첨가하여 9주 동안 투여한 후 간장의 무게, 혈청 효소활성도 및 간세포의 미세구조를 투과형전자현미경(TEM)으로 관찰하였다. HF군은 정상군의 간 무게에 비하여 약 35% 증가된 반면에 T5군의 경우는 HF군에 비하여 약 13% 감소하였다. 혈청 중 GOT 활성도는 정상군과 HF군이 비슷한 수준이었으나 T1군과 T5군에서는 HF군에 비하여 각각, 6.1%, 17.8% 감소하였으며 혈청 중 GPT 활성도 역시 정상군과 HF군이 거의 비슷한 수준이었으나 T1군과 T5군에서는 HF군에 비하여 각각 25.4, 32.4% 감소하였다. 간세포조직의 지방축적 정도 및 형태적 특징을 전자현미경으로 관찰한 결과, 정상군의 모양은 원형 내지 다각형을 나타내고 핵은 크고 둥글며 세포의 중앙에 위치해 핵질이 고르게 발달해 있을 뿐만 아니라 세포질에는 미토콘드리아를 비롯한 각종 세포 소기관이 고루 분포되어 있었으며 세포 전반에 걸쳐 지방구는 보이지 않았다. HF군은 세포전반에 걸쳐 지방구에 뒤덮여 세포의 형태 관찰이 불가능할 정도였으며 지방의 과잉 축적으로 인한 전자밀도가 낮아 백색을 나타내어 세포소기관의 막과 형태가 불분명한 지방간의 심각한 양상을 볼 수 있었다. T1군은 HF군에 비하여 지방구가 많이 소멸되었으며, T5군은 T1군에 비하여 지방세포의 크기와 수가 현저히 줄어든 것이 관찰 되었고, 더욱더 선명한 핵과 핵막 및 세포질의 전자밀도가 높아 지방질의 감소를 볼 수 있었다.

• 동의생리병리학회지
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• 제26권1호
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• pp.10-18
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• 2012

In this paper, to find out how different the treatment of special individuals named 'old people' is with that of ordinary cases, Dongeuibogam(東醫寶鑑) was chosen as the study material and some parts in it where the symptoms of old people in the same disease category were treated in different ways were excerpted to analyze the tendency of pathological symptoms and prescriptions in the physiological perspective. As the result of analysis on the parts in Dongeuibogam(東醫寶鑑) where the old people were treated in different ways, it turned out that 65 prescriptions were used in 24 pathological symptoms. The 24 symptoms are included mainly in chapters of 'inner view(內景)' and 'outer form(外形)'. They are including the pathological symptoms which had been presented as general geriatric symptoms and also seems to have added other symptoms which should be clinically reflected in the specialty of treatment for geriatric diseases. The 65 prescriptions are also recorded mainly in chapters of 'inner view(內景)' and 'outer form(外形)'. The herbs used for them were sweet and warm herbs such as Rehmannia glutinosa (Gaertner) Libosch(熟地黃), Angelica gigas Nakai(當歸), Panax ginseng C. A. Mey(人蔘) and Atractylodes macrocephala Koidz(白朮) that can support the essence blood of liver and kidney and the energy of spleen, lung and stomach. Those herbs could be added or subtracted according to the symptom. It seems to have resulted from the treatment method that old people was applied to old people in consideration of their physiological features. When the 24 symptoms and 65 prescriptions for geriatric diseases different from ordinary ones in Dongeuibogam(東醫寶鑑) were considered in the physiological view point, it could be seen that each pathological symptom was manifested as a exhaustion of kidney qi(腎氣枯渴) which was a characteristic physiologic state of old people. Through this, it could be seen that the use of prescriptions was also made to mitigate the body fluid deficiency(津液不足), the kidney yin inner heat(腎陰內熱), the heart spirit void and loss(心神虛損), the kidney yang deficiency(腎陽不足), the spleen failing in transportation(脾失健運), the spleen yang deficiency(脾陽不足), the liver fire inner movement(肝火內動) and the lung energy void and loss(肺氣虛寒).