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Factors Affecting Introduction of rolC Gene in Lycium chinense Mill. (구기자나무(Lycium chinense Mill.)로의 rolC유전자 도입에 미치는 요인)

  • 박용구;최명석;김병원;정원일;노광수
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.6
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    • pp.329-334
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    • 1995
  • Transformation system of rolC gene, dwarf gene in Lycium chinenese Mill. established by using system. Pin-punctured leaves induced numerous adventious buds in abaxial side when cultured on 3/2 MS medium containing 2.0 mg/L zeatin. Survival rate and shoot regeneration frequency of leaf explants decreased as kanamycin sulfate level increased. Shoot buds were not regenerated on 3/2 MS medium containing 10 mg/L kanamycin sulfate and 2.0 mg/L zeaein. Of the level tested, 10 mg/L of kanamycin sulfate was optimum in selection of kanamycin sulfate resistant plant. Co-culture time of bacteria and leaf explants was affected at the frequency of shoot regeneration and survival of leaf explants. Leaf explants co-cultivated during above 48hr severely decreased survival rate and shooting rate. Best result on survival rate and shooting rate were obtained when exposed for 24 h. 80 explants of 105 leaf explants survived on 3/2 MS medium containing 2.0 mg/L zeatin and 10 mg/L kanamycin sulfate, and 15 shoots was regenerated on the same medium. To select kanamycin sulfate resistant plant, regenerate as cultured on 3/2 MS medium containing 10 mg/L kanamycin sulfate, and obtained 5 kanamycin resistant plants. Southern blot analysis conformed that the rolC gene was incorporated into the genomic DNA of kanamycin resistant plants.

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A Clinical Study of Femur Neck Fractures (대퇴 경부 골절의 임상적 고찰)

  • Koo, Hwan-Mo;Ahn, Myun-Whan;Ihin, Joo-Choul
    • Journal of Yeungnam Medical Science
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    • v.7 no.1
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    • pp.69-80
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    • 1990
  • A clinical analysis was done on 23 patients(24 hips) with fracture of the femoral neck, who had been admitted and treated at our Orthopedic department during the period of 4 years, from Jan, 1984 to May 1988. The results were as follows : 1. 23 patients were comprised of 4 males and 19 females, and 11 patients were over 65 years old. 2. 18 cases of 24 cases were due to minor traumas such as slipping down, and for over 65 years old, all cases were due to simple minor traumas, 8 cases were showed a severe osteoporosis, below grade 3 of the Singh's index. 3. 14 cases of 24 cases were displaced subcapital fractures, and 6 cases displaced transcervical fractures. Only 4 cases were the undisplaced transcervical fractures. 4. In treatment of fractures internal fixations after manipulation were performed in 14 cases and primary arthroplasties in 10 cases. Secondary arthroplasties were done in complicated 4 cases of 14 cases treated with internal fixations. 5. Complications after internal fixation were developed in 7 cases out of 14 cases, avascular necrosis in 6, nonunions in 2, pin migrations in 3, and metal failure in 1 case. 6. In 14 arthroplasty immediate surgical fitness of femoral stem were related to late loosening of femoral stem(correlation coefficient r=-0.68, p<0.01).

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Experimental Study on the DPF Engine Oil Characteristics Depending on a Mileage of Diesel Automotive (디젤차량의 주행거리에 따른 DPF 윤활유의 특성분석에 관한 실험적 연구)

  • Kim, Chung-Kyun
    • Tribology and Lubricants
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    • v.25 no.5
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    • pp.318-323
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    • 2009
  • The oil characteristics and wear particles of Diesel engines with a DPF have been investigated as a function of a driving distance. The engine oil of SAE 5W30 with ACEA C3 is used for an oil film lubrication of the engine, which is equipped with Diesel particulate filter. Depending on the oil test results, the kinematic viscosity of used engine oils at 40 is degraded up to 5.1% compared with that of unused engine oils, SAE 5W30. And the kinematic viscosity of used engine oils at 100 is more degraded up to 8.1% compared with that of unused engine oils. The oil characteristic as a function of a mileage is not changed depending on the driving distance because of high quality of engine oils. But the aluminum and copper compounds, which are used as base materials of the engine bearing and a pin bush, are much worn and contaminated for the increased mileage of the car. The oil properties of used engine oils are relatively good except phosphorus and calcium additives, which are heavily engaged in the performance of the oils.

Coracoclavicular Screw Fixation for AC Dislocation and Unstable Distal Clavicle Fracture (견봉 쇄골 탈구와 쇄골 외측단 골절에서 일시적 오구 쇄골간 나사못 고정술)

  • Kim Jin Sub;Jun Jin Ho;Chung Yung Khee
    • Clinics in Shoulder and Elbow
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    • v.2 no.2
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    • pp.133-137
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    • 1999
  • Purpose: There are so many different methods with the their own advantages and disadvantages for acute AC dislocation and distal unstable clavicle fracture. We evaluated the usefulness of temporary Bosworth's coracoclavicular fixations using the cannulated screw and reviewed our clinical results. Materials and Methods: We evaluated 34 cases of temporary coracoclavicular fixations with cannulated screw and washer for acute AC dislocation(25cases in the Type V, Ⅲ) and distal unstable clavicle fracture(9cases in the Type ⅡB, Ⅱ+Ⅲ). All cases were fixed by the same techniques, guided cannulated screw under C-arm after the repair of the CC ligament within 1 week. Bases on the regular check, screws were removed at the 6-12 weeks under local anesthesia. The final clinical and radiological results(average 14 months follow-up) were rated with the UCLA scale. Results : All shoulders were gained nearly full ROM passively at the 3-4 weeks. The overall satisfactory resuIts(excellent or good) were achieved 88%(22/25) in the acute AC dislocation and all in the distal unstable clavicle fracture. There were no definite complications except three initial fixations loosening. Conclusion: Temporary Bosworth's coracoclavicular fixation using the cannulated screw with guided pin was simple precedure and provided enough initial stability for acute AC dislocation and distal unstable clavicle fracture.

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Design of Broadband Planar Monopole Antenna (광대역 평면형 모노폴 안테나의 설계)

  • Lee Yun-Kyung;Yoon Hyun-Bo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.16 no.4 s.95
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    • pp.359-365
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    • 2005
  • This paper designed a very low profle, light and broadband internal antenna for operating at PCS, IMT-2000 and Wibro bands. The proposed antenna can be reduced the size by using shorting-pin and a broadband characteristic is obtained by using slit. It is optimized by using the CST Microwave Studio commercial software based on the FIA(Finite Integration Algorithm) and PBA(Perfect Boundary Approximation) and then fabricated and measured. As a result of measurement, the bandwidth(VSWR<2.5) is $40.8\;\%$ at $1.934\;\cal{GHz}$ and the size of antenna is 3$30\;\cal{mm}\times10\;\cal{mm}\times0.2\;\cal{mm}$.

Treatment and Rehabilitation in a Common Kestrel (Falco tinnunculus) with Both Legs Broken (양쪽 다리 골절 황조롱이의 치료와 재활 1례)

  • Kim, Hee-jong;Kim, Young-jun;Kim, Moon-jung;Park, Young-seok;Kim, Byeong-su;Park, Seong-jun
    • Journal of Veterinary Clinics
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    • v.36 no.1
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    • pp.82-84
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    • 2019
  • A common kestrel (Falco tinnunculus) was presented with inability to stand and fly at Chungnam Wild Animal Rescue Center. The kestrel showed non-weight bearing ambulation on both legs and crepitation with no external wound. Radiographs revealed a fracture on mid-diaphysis of right tibiotarsus and a distal end fracture of left femur. Intramedullary pinning was chosen for reduction of both fractures. The right tibiotarsal fracture was corrected first, and left femur was repaired 5 days later. The kestrel was managed with a specially designed sling to prevent further iatrogenic damage for 2 weeks. Three weeks after the surgery, the kestrel was able to stand and found to perch in 4 weeks. On 5 weeks, The kestrel could fly free at outdoor aviary and was released through rehabilitation for 3 months finally.

The Effect of Complexing Agents on the Deposit Characteristics in the Electroless Nickel-Tungsten-Phosphorus Plating (무전해 Ni-W-P 도금에서 착화제의 종류가 피막특성에 미치는 영향)

  • Cho, Jin Ki;Park, Sang Wook;Kang, Seung Goon;Son, Seong-Ho
    • Korean Journal of Metals and Materials
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    • v.46 no.11
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    • pp.725-729
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    • 2008
  • Deposition characteristics of electroless plated Ni-W-P films were investigated for various complexing agents. Used complexing agents are sodium citrate, sodium gluconate and sodium malonate. In this study, the existing mixed potential theory could explain the overall mechanism of Ni-W-P electroless plating for all complexing agents. The deposition rate could be also expected by the theory. The deposited Ni-W-P films were evaluated in term of surface hardness and corrosion resistance. Microhardness of the deposit increased about 1,000 Hv after heat treatment for one hour at $400^{\circ}C$, because it was above the crystallization temperature of $Ni_3P$. The deposited Ni-W-P films can exhibit excellent corrosion resistance in using sodium malonate as a complexing agent, the other hand the using sodium gluconate was the worst corrosion resistance. The worst corrosion resistance was due to a large number of nano-sized pin-holes or small pores. The plating current at the mixed potential increases when the using sodium malonate as a complexing agent, it was explained by the cross section.

Neutronics modelling of control rod compensation operation in small modular fast reactor using OpenMC

  • Guo, Hui;Peng, Xingjie;Wu, Yiwei;Jin, Xin;Feng, Kuaiyuan;Gu, Hanyang
    • Nuclear Engineering and Technology
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    • v.54 no.3
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    • pp.803-810
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    • 2022
  • The small modular liquid-metal fast reactor (SMFR) is an important component of advanced nuclear systems. SMFRs exhibit relatively low breeding capability and constraint space for control rod installation. Consequently, control rods are deeply inserted at beginning and are withdrawn gradually to compensate for large burnup reactivity loss in a long lifetime. This paper is committed to investigating the impact of control rod compensation operation on core neutronics characteristics. This paper presents a whole core fine depletion model of long lifetime SMFR using OpenMC and the influence of depletion chains is verified. Three control rod position schemes to simulate the compensation process are compared. The results show that the fine simulation of the control rod compensation process impacts significantly the fuel burnup distribution and absorber consumption. A control rod equivalent position scheme proposed in this work is an optimal option in the trade-off between computation time and accuracy. The control position is crucial for accurate power distribution and void feedback coefficients in SMFRs. The results in this paper also show that the pin level power distribution is important due to the heterogeneous distribution in SMFRs. The fuel burnup distribution at the end of core life impacts the worth of control rods.

Sliding Wear Properties of Carbon Fiber Reinforced $Si_3N_4$ Ceramics (탄소섬유강화 질화규소 세라믹스의 마찰마모 특성)

  • Park Yi-Hyun;Yoon Han-Ki;Kim Bu-Ahn;Park Won-Jo
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2004.05a
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    • pp.347-351
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    • 2004
  • [ $Si_3N_4$ ] composites have been extensively studied for engineering ceramics, because it has excellent room and high temperature strength, wear resistance properties, good resistance to oxidation, and good thermal and chemical stability. In the present work, carbon short fiber reinforced $Si_3N_4$ ceramics were fabricated by hot press method in $N_2$ atmosphere at $1800^{\circ}C$ using $Al_2O_3\;and\;Y_2O_3$ as sintering additives. Content of carbon short fiber was $0\%,\;0.1\%\;and\;0.3\%$. The composites were evaluated in terms of density, flexural strength and elastic modulus through the 3-point bending test at room temperature. Also, The wear behavior was determined by the pin on disk wear tester using silicon nitride ball. Experimental density and flexural strength decreased with increasing content of carbon fiber. But specific modulus increased with increasing content of carbon fiber. In addition, friction coefficient and specific wear loss decreased with increasing content of carbon short fiber by reason of interfacial defects between matrix and fiber.

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Effect of Electrode Placement on Procaine Iontophoresis (전극배치가 Procaine 이온도입에 미치는 영향)

  • Lee, Jae-Hyoung;Song, In-Young;Kook, Jee-Youn
    • Journal of Korean Physical Therapy Science
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    • v.4 no.2
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    • pp.399-403
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    • 1997
  • The purpose of this study was to determine the efficacy of electrode placement on procaine iontophoresis. Thirty-three healthy students with an age range of 19 to 34 years, were participated in this study. The subjects were randomly assigned into 3 groups. Each subjects received iontophoresis on the proximal 1/3 of volar surface of dominant forearm with soft cotton pad($3.5{\times}3.5cm$) soaked in 2 ml of 4% procaine hydrochloride (pH 5.1) at 4 mA for 10 minutes(total current 40 mA min) of anodal DC. In transversal electrode placement(TEP) group, dispersive electrode was placed on the proximal 1/3 of dorsal surface of the forearm. In longitudinal electrode placement (LEP) group and control group, dispersive electrode were placed on the distal 1/3 of volar surface of the forearm. After procaine iontophoresis, duration of anesthesia were evaluated at five minutes intervals on five random locations in the iontophoretically area using a 21-gauge sterile hypodermic needle pressed with 1 mm invagination until return the sharp pin-pricking pain sensation. The data were ana lysed with one-way ANOVA to determine signific~nt differences between groups. The results showed significantly differences in the local anesthetic duration between the 3 groups(p<0.001). The anesthetic durations of TEP group and LEP group were significantly longer when compared with control group(p<0.05). Anesthetic durations of TEP group and LEP group were not significantly difference, but anesthetic duration of LEP group tends to longer than TEP group. In view of these results, clinicians should consider the electrode placement method when performing the iontophoresis.

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