• Journal of the Computational Structural Engineering Institute of Korea
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• v.17 no.3
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• pp.279-293
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• 2004

The analysis of linear static and free vibration problems of isotropic and laminated composite plates and shells is performed by the improved 9-node shell element with the new strain displacement relationship. In that relationship, the effect of new additional terms between the bending strain and displacement has been investigated in the warping problem. Natural co ordinate based strains, stresses and constitutive equations are used. The assumed natural strain method is used to alleviate both membrane and shear locking behavior from the element. The Lanczos method is employed in the calculation of the eigenvalues of laminated composite structures and the Gauss integration rule is adopted to evaluate the mass matrix. The numerical examples are compared with the analytical solutions to validate the current formulation and the results presented could be useful for the understanding of the behaviour of laminates under free vibration conditions.

• Journal of the Korean Society of Hazard Mitigation
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• v.7 no.5
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• pp.47-60
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• 2007

In this paper, we investigate the natural frequencies and buckling loads of functionally graded material (FGM) plates and shells, using a quasi-conforming shell element that accounts for the transverse shear strains and rotary inertia. The eigenvalue of the FGM plates and shells are calculated by varying the volume fraction of the ceramic and metallic constituents using a sigmoid function, but their Poisson's ratios of the FGM plates and shells are assumed to be constant. The expressions of the membrane, bending and shear stiffness of FGM shell element are more complicated combination of material properties than a homogeneous element. In order to validate the finite element numerical solutions, the Navier's solutions of rectangular plates based on the first-order shear deformation theory are presented. The present numerical solutions of composite and sigmoid FGM (S-FGM) plates are proved by the Navier's solutionsand various examples of composite and FGM structures are presented. The present results are in good agreement with the Navier's theoretical solutions.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.4
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• pp.684-695
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• 1992

The present study is concerned with the analysis of three-dimensional sheet metal forming process by the upper-bound method. For the analysis a systematic approach is necessary for the expression of geometric configuration of the deforming workpiece. In the present paper geometric configuration is constructed by three unit surfaces which are defined by sweeping the vertical section curves and boundary curve. The principal components of strain increment during the process is calculated directly from the change of geometric configuration for an arbitrary triangular element. The corresponding solution is found through optimization of the total energy consumption with respect to some parameters assumed in the velocity field and geometric profile. In order to verify the effectiveness of the present method, hydrostatic bulging of a rectangular disphragm is analyzed and the computation by the present method for the geometric shape renders the good result. From the comparison of the present results with the existing experimental results and elastic-plastic finite element solutions, good agreements have been obtained for the pressure curves, polar membrane strains and pressure distributions. The present method can thus be further applied to the analysis of other three-dimensional sheet metal forming processes.

• The Korean Journal of Ecology
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• v.22 no.1
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• pp.39-43
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• 1999

Serratia marcescens, an enterobacterium of gram-negative bacteria, is characterized by resistance of the admium. Cadmium sensitive PM strain did not grow in the medium at cadmium concentration of 50 ppm. PA strain was induced to accommodate to cadmium by cultivating the mother strain (PC strain) in the medium with 50 ppm cadmium. As compared with PC and PM strains, PA strain revealed the excellent growth in cadmium media and accumulated four to five times higher cadmium concentration in cell than other strains. PA strain accumulated 23% of cadmium in cells when cultured in medium treated with 100 ppm cadmium and this cadmium was more accumulated in cytosol fractions than membrane fractions. Analysis by TEM indicated that cadmium was concentrated as a form of granule in cytosol. In protein patterns of cell after the treatment of cadmium, two inducible proteins (28 KDa and 64 KDa) and one reducible protein (45 KDa) were detected by SDS-PAGE. By Atomic Absorption Spectrophotometer, the amounts of cadmium attached to inducible proteins of 28 KDa and 64 KDa were 318.28 ㎍ and 325.37 ㎍ per gram of protein, respectively. It is assumed that these inducible proteins play an important role in the mechanism of cadmium accumulation in cells. A plasmid of 23Kbp was found in S. marcescens. The ability of resistance to cadmium in plasmid was confirmed by curing experiments.

• Parasites, Hosts and Diseases
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• v.33 no.4
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• pp.349-356
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• 1995

We have investigated the action of sodium nitrite on the growth and morphologic changes of T uaginolis and on the treatment of subcutaneous abscess by trichomonad in mice. Sodium nitrite inhibited the growth of metronidazole-sensitive KT9 isolate and metronidazole-resistant CDC85 strain of T vcsinalis as concentration of 6 mM and 10 mM respectively Intraperitoneal injection of sodium nitrite (70 ㎍, 100 ㎍, 130 ㎍/g body weight) did not reduce the size of abscess produced by subcutaneous inoculation of T uasinnlis in mice. T uosinnlis, treated with sodium nitrite at concentration giving about 50% inhibition of growth, showed fissures, many vacuoles and electron-translucent zone in the cytoplasm by transmission electron microscopy. In the case of CDC85 treated with 9 mM sodium nitrite, hydrogenosomal matrical change , destruction of hydrogenosomal membrane, autophagic vacuoles, disappearance of Golgi complex and polysome were notably observed . With above results, it is assumed that sodium nitrite inhibits the growth of metronidazole-sensitive and - resistant strains of T. ucsinalis and induces the morphological changes of 7 uusinalis although it does not affect in reducing of abscess size by vagiginalis in mice.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.384-386
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• 2014

Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220 colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to 1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to 1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as more precise and improvable method for identification of P. aeruginosa.