• The Plant Pathology Journal
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• v.24 no.3
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• pp.289-295
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• 2008

RNA-RNA interactions and the dynamic RNA conformations are important regulators in virus replication in several RNA virus systems and may also involved in the regulation of many important virus life cycle phases, including translation, replication, assembly, and switches in these important stages. The 5' non-translated region of Potato virus X(PVX) contains multiple cis-acting elements that facilitate various viral processes. It has previously been proposed that RNA-RNA interactions between various RNA elements present in PVX RNA genome are required for PVX RNA accumulation(Hu et al., 2007; Kim and Hemenway, 1999). This model was based on the potential base-pairing between conserved sequence elements at the upstream of subgenomic RNAs(sgRNAs) and at the 5' and 3' end of RNA genome. We now provide more evidence that RNA-RNA base-pairing between elements present at the 5' end and upstream of each sgRNA is required for efficient replication of genomic and subgenomic plus-strand RNA accumulation. Site-directed mutations introduced at the 5' end of plus-strand RNA replication defective mutant(${\Delta}12$) increasing base-pairing possibility with conserved sequence elements located upstream of each sgRNAs restored genomic and subgenomic plus-strand RNA accumulation and caused symptom development in inoculated Nicotiana benthamiana plants. Serial passage of a deletion mutant(${\Delta}8$) caused more severe symptoms and restored wild type sequences and thus retained possible RNA-RNA base-pairing. Altogether, these results indicate that the RNA element located at the 5' end of PVX genome involved in RNA-RNA interactions and play a key role in high-level accumulation of plus-strand RNA in vivo.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.6
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• pp.512-519
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• 2004

This paper described the free vibration characteristics of Optimized H Type (OHT) spacer grids (SG) supporting the PWR fuel rod. The vibration test and the finite element (FE) analysis are performed under the free boundary condition and the clamped at two points (or three points) in the bottom which is the same one as the experimental condition for the dummy rod continuously supported by spacer grids. A modal test is conducted by the impulse excitation method using an impulse hammer and an accelerometer, and the TDAS module of the I-DEAS software is used to acquire and analyze the sensor signals. The softwares related to the FE analysis are the I-DEAS for the geometrical shape modeling and meshing, and the ABAQUS for solving. The fundamental frequency of the OHT SG by experiment under a clamped condition at two points is 175.18 Hz, and shows a bending mode. We think there is no resonance between the fuel rod and the SG because the SG's frequency is higher than that of the fuel rod existing in the range from 30 to 120 Hz. The fundamental frequency of the SG under the free boundary condition is 349.2 Hz showing a bending mode, and the results between the test and the analysis have a good agreement with maximum 7 ％ in error It is also found that the FE analysis model of the OHT SGs to analyze an impact, a buckling and vibration et al. has been generated with reliability.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.53.1-53.1
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• 2018

During a major solar eruption, the erupting plasma is possibly out of the equilibrium ionization state because of its rapid heating or cooling. The non-equilibrium ionization process is important in a rapidly evolving system where the thermodynamical time scale is shorter than the ionization or recombination time scales. We investigate the effects of non-equilibrium ionization on EUV and X-ray observations by the Atmospheric Imaging Assembly (AIA) on board Solar Dynamic Observatory and X-ray Telescope (XRT) on board Hinode. For the investigation, first, we find the emissivities for all the lines of ions of elements using CHIANTI 8.07, and then we find the temperature responses multiplying the emissivities by the effective area for each AIA and XRT passband. Second, we obtain the ion fractions using a time-dependent ionization model (Shen et al. 2015), which uses an eigenvalue method, for all the lines of ion, as a function of temperature, and a characteristic time scale, $n_et$, where $n_e$ and t are density and time, respectively. Lastly, the ion fractions are multiplied to the temperature response for each passband, which results in a 2D grid for each combination of temperature and the characteristic time scale. This is the set of passband responses for plasma that is rapidly ionized in a current sheet or a shock. We investigate an observed event which has a relatively large uncertainty in an analysis using a differential emission measure method assuming equilibrium ionization state. We verify whether the observed coronal plasmas are in non-equilibrium or equilibrium ionization state using the passband responses.

• Journal of Broadcast Engineering
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• v.2 no.1
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• pp.8-15
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• 1997

In this paper, a real-time operating Motion Picture Experts Group-1 (MPEG-1) audio encoder system is implemented using a TMS320C31 Digital Signal Processor (DSP) chip. The basic operation of the MPEG-1 audio encoder algorithm based on audio layer-2 and psychoacoustic model-1 is first verified by C-language. It is then realized using the Texas Instruments (Tl) assembly in order to reduce the overall execution time. Finally, the actual BSP circuit board for the encoder system is designed and implemented. In the system, the side-modules such as the analog-to-digital converter (ADC) control, the input/output (I/O) control, the bit-stream transmission from the DSP board to the PC and so on, are utilized with a field programmable gate array (FPGA) using very high speed hardware description language (VHDL) codes. The complete encoder system is able to process the stereo audio signal in real-time at the sampling frequency 48 kHz, and produces the encoded bit-stream with the bit-rate 192 kbps. The real-time operation capability of the encoder system and the good quality of the decoded sound are also confirmed using various types of actual stereo audio signals.

• Journal of Korean Society on Water Environment
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• v.26 no.1
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• pp.168-176
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• 2010

This study proposes the improvement of the present Total Maximum Daily Load (TMDL) management system of MOE (Ministry of Environment). The margin of safety (MOS) is calculated by a method using standard error and a method using variability and uncertainty. The allocation of pollutant loads are calculated using three methods, equal load reduction method, equal percent removal method and method using equity standards. This study applied the improved TMDL management system to the Anyangcheon watershed. Since MOS varies from 12% to 44% due to the high variability of measured and simulated data, it must not be ignored in the TMDL. The method using equity standards is the most proper in this application since the others produced unrealistic allocations. Area, runoff, water use quantity, population and budget are considered for equity standards. This study shows that this allocation method can be also applicable for the administrative units as well as the sub-watersheds. Finally, Hydrologic Simulation Program-FORTRAN (HSPF) with the allocated pollutant load was used to confirm whether it satisfy the water quality standard or not. This study will be helpful to improve the MOS and allocation system TMDL in the future.

• Molecules and Cells
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• v.23 no.2
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• pp.228-238
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• 2007

Since molecular structure of hnRNP is not available in foreseeable future, it is best to construct a working model for hnRNP structure. A geometric problem, assembly of $700{\pm}20$ nucleotides with 48 proteins, is visualized by a frame work in which all the proteins participate in primary binding, followed by secondary, tertiary and quaternary binding with neighboring proteins without additional import. Thus, 40S hnRNP contains crown-like secondary structure (48 stemloops) and appearance of 6 petal (octamers) rose-like architectures. The proteins are wrapped by RNA. Co-transcriptional folding for RNP fibril of FMR1 gene can produce 2,571 stem-loops with frequency of 1 stem-loop/15.3 nucleotides and 53 40S hnRNP beaded structure. By spliceosome driven reactions, there occurs removal of 16 separate lariated RNPs, joining 17 separate beaded exonic structures and anchoring EJC on each exon junction. Skipping exon 12 has 5'GU, 3'AG and very compact folding pattern with frequency of 1 stem-loop per 12 nucleotides in short exon length (63 nucleotides). 5' end of exon 12 contains SS (Splicing Silencer) element of UAGGU. In exons 10, 15 and 17 where both regular and alternative splice sites exist, SS (hnRNP A1 binding site) is observed at the regular splicing site. End products are mature FMR-1 mRNP, 4 species of Pri-microRNAs derived from introns 7,9,15 and 3'UTR of exon17, respectively. There may also be some other regulatory RNAs containing ALU/Line elements as well.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.3
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• pp.173-180
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• 2009

After the UN General Assembly on 2006, Interest on the marine litter has rapidly increased internationally. The UN sub-organizations, UNEP/Regional Seas, FAO(Food and Agriculture Organization of the United Nations) and OC(Ocean Conservancy) as a one of world NGO had been tackled to collect information and prepared world reports related on marine litter, recently, those are published. Through the 10years experience of Korea's policy on marine litter since 1999, it is re-evaluated as a roll model internationally. In this paper, brief introduction of structure and function of Korea's authorities which are responsible for or are involved in the marine litter issue as well as the National Marine Litter Management Basic Plan which is established in 2008, has been provided. This paper also included the structure and roll of the marine litter initiative center in the plan. Change of the paradigm of action plan on marine litter in Korea already had been started.

• Explosives and Blasting
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• v.22 no.4
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• pp.7-15
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• 2004

An explosion modeling technique was developed by using the spherical discrete element code, PFC3D, which can be used to model the dynamic stress wave propagation phenomenon. The modeling technique is simply based on an idea that the explosion pressure should be applied to a PFC3D particle assembly not in the form of an external force (body force), but in the form of a contact force (surface force). According to this concept, the explosion pressure is applied to the wall particles by the scheme of radius expansion/contraction of inner-hole particles. The output wall force is compared to the input hole pressure in every time step, and a correction routine is activated to control the radius multiplier of the inner-hole particles. A comparative blast simulation far a cement mortar block of $80\times90\times80mm$ was conducted by using the conventional explosion modeling method and the new one. The results of the simulation are presented in a qualitative fashion.

• Korean Journal of Construction Engineering and Management
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• v.13 no.1
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• pp.53-66
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• 2012

Gangform compared with conventional wood and steel form is systemized form which has a capability to install and dismantle form at a time without repeating to assembly and disassembly small members. Gangform compared with ACS has some problems such as 1) Tower Crane load increase, 2) increase in safety incidence during climbing Gangform, 3) decrease in detachment operation productivity, 4) stop work due to climate impacts, and 5) decrease in labor cost, productivity, quality. A conceptual design model of automated Gangform climbing system is suggested for apartment housing construction to show its technical/economic feasibility and workers' safety while increasing operation productivity and concrete quality.

• Journal of the Korean GEO-environmental Society
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• v.13 no.10
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• pp.55-62
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• 2012

In this research, the algorithm for simulating specific grain size distribution(GSD) with large diameter granular material was developed using the distinct element analysis program $PFC^{3D}$(Particle Flow Code). This modeling approach can generate the initial distinct elements without clump logic or cluster logic and prevent distinct element from escaping through the confining walls during the process. Finally the proposed distinct element model is used to simulate large triaxial compression test of the rockfill material and we compared the simulation output with lab test results. Simulation results of Assembly showed very well agreement with the GSD of the test sample and numerical modeling of granular material would be possible for various stress conditions using this application through the calibration.