• Title/Summary/Keyword: Aspergillus flavus

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Effects of Plant Extracts on Conidial Germination, Mycelial Growth and Sporulation of Fungi Isolated From Poultry Feed

  • Islam, M. Rafiqul;Alam, Shahidul;Rahman, M. Ziaur;Chowdhury, S.P.;Begum, M.F.;Akhter, Nargis;Alam, M.S.;Han, Kee-Don;Lee, Min-Woong
    • Mycobiology
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    • v.31 no.4
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    • pp.221-225
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    • 2003
  • Effect of ethanolic extracts of Lawsonia inermis, Azadirachta indica, Vinca rosea, Tagetes patula, Ocimum sanctum, Colocasia antiquorum, Adhatoda vasica, Moringa oleifera, Datura metel and Curcuma longa leaf on conidial germination, mycelial growth and sporulation of Aspergillus flavus, A. niger and A. fumigatus were examined. The conidial germination of A. flavus and A. fumigatus were most inhibited by the extract of L. inermis, while that of A. niger was inhibited by A. indica. Other tested plant extracts have a good effect on conidial germination on the selected fungi. The highest mycelial growth of A. flavus(37 mm) was found in V. rosea, but in case of A. niger and A. fumigatus it(38 and 39 mm) was found in D. metel. The lowest(4, 9 and 6 mm) respectively mycelial growth of these fungi found in L. inermis. The highest sporulation($75{\times}10^4/ml$) of A. flavus was counted in V. rosea, but in case of A. niger and A. fumigatus those($45{\times}10^4$ and $55{\times}10^4/ml$) were in D. metel and the lowest($5{\times}10^4,\;12{\times}10^4\;and\;9{\times}10^4/ml$) respectively sporulation of these fungi counted in L. inermis plant extract medium.

Cellulase activity of Aspergilli distributed in South Korea(I) (한국산 Aspergilli의 셀룰라아제 활성에 관한 연구 1)

  • 이영녹;김낙정;서항원
    • Korean Journal of Microbiology
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    • v.14 no.3
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    • pp.105-116
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    • 1976
  • 410 strains which belong to the genus aspergillus were isolated from specimens collected throught South Korea, and 17 species nad 1 variety through the 13 species groups were identified according to Repaer-Fennell's classification key. Among them the species and variety unrecorded in Korea are as follows : A, giganiteus, A. pseudogloucus, A. spinulosus, A.ficuum, A.japonicus, A.flavus var. columnaris, A. flavipes.

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Electron Microscopic Studies on the Cellular Changes in the Liver of Ducklings Induced by Korean Aflatoxin B$_1$ (한국산 Aflatoxin이 오리새끼의 간장에서 유발시킨 병변에 관한 전자현미경적 연구)

  • Lee Kwang-Juing;Yoon Hwa-Juing;Lee In-ho
    • Journal of the korean veterinary medical association
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    • v.26 no.9
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    • pp.543-553
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    • 1990
  • This report describes the cellular changes induced in the livers of ducklings by a sin91e administration of aflatoxin B$_1$ produced by Korean Industrial Strain of the Aspergillus flavus, in order to examine the toxicity of the minimum dose of

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Studies on Mycotoxins in Feed (사료내의 곰팡이 독소에 관한 연구)

  • Yoon Hwa-Joong;Chang Kyoung-Jin;Kim Tae-Jong
    • Journal of the korean veterinary medical association
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    • v.19 no.7
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    • pp.67-73
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    • 1983
  • Strains of Aspergillus flavus produced aflatoxin $B_1$ was found in the corns for eed and cultured them in the incubator. The aflatoxin $B_1$ was produced from the Aspergilli and intubated into the pharynx of the ducklings for the to

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Mycoflora in Broiler Houses (Broiler양계사내(養鷄舍內)의 진균총(眞菌叢))

  • Choi, Won-pil;Yeo, Sang-geon;Lee, Hun-jun
    • Korean Journal of Veterinary Research
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    • v.24 no.1
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    • pp.36-39
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    • 1984
  • The present survey was undertaken to determine the mycoflora of broiler houses. Attempts were made to isolate and identify fungi in the dust, feed, litter and water from 21 broiler houses. A total of 166 isolates of fungi was identified as yeast spp. (44%), Aspergillus spp. (30.7%), Verticillium spp. (7.2%), Penicillium spp. (3.6%), Paecilomyces spp. (3.6%), Scopulariopsis spp. (3.0%), Cephalosporium spp. (3.0%), Chrysosporium spp. (2.4%), Cladosporium spp. (1.8%) and Absidia spp. (0.6%). Isolated of Aspergillus(A) spp. and Penicillium(P) spp. were identified as A. fumigatus, A. flavus, A. terreus, A. nidulans, A. niger, P. citrinum and P. palitan. Fungal contamination of the broiler houses predominated in the dust. The important point is that must of the isolates were pathogenic fungi.

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Antifungal Activities of Biorelevant Complexes of Copper(II) with Biosensitive Macrocyclic Ligands

  • Raman N.;Joseph J.;Velan A. Senthil Kumara;Pothiraj C.
    • Mycobiology
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    • v.34 no.4
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    • pp.214-218
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    • 2006
  • Four copper(II) complexes have been prepared using macrocyclic ligands. The macrocyclic ligands have been synthesized by the condensation reaction of diethyl phthalate with Schiff bases derived from o-phenylene diamine and Knoevenagel condensed ${\beta}-ketoanilides$ (obtained by the condensation of acetoacetanilide and substituted benzaldehydes). The ligands and copper complexes have been characterized on the basis of Microanalytical, Mass, UV-Vis., IR and CV spectral studies, as well as conductivity data. On the basis of spectral studies, a square-planar geometry for the copper complexes has been proposed. The in vitro antifungal activities of the compounds were tested against fungi such as Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans. All the synthesized copper complexes showed stronger antifungal activities than free ligands. The minimum inhibitory concentrations (MIC) of the copper complexes were found in the range of $8{\sim}28\;{\mu}g/ml$. These compounds represent a novel class of metal-based antifungal agents which provide opportunities for a large number of synthetic variations for modulation of the activities.

Microbe-Mediated Control of Mycotoxigenic Grain Fungi in Stored Rice with Focus on Aflatoxin Biodegradation and Biosynthesis Inhibition

  • Mannaa, Mohamed;Kim, Ki Deok
    • Mycobiology
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    • v.44 no.2
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    • pp.67-78
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    • 2016
  • Rice contaminated with fungal species during storage is not only of poor quality and low economic value, but may also have harmful effects on human and animal health. The predominant fungal species isolated from rice grains during storage belong to the genera Aspergillus and Penicillium. Some of these fungal species produce mycotoxins; they are responsible for adverse health effects in humans and animals, particularly Aspergillus flavus, which produces the extremely carcinogenic aflatoxins. Not surprisingly, there have been numerous attempts to devise safety procedure for the control of such harmful fungi and production of mycotoxins, including aflatoxins. This review provides information about fungal and mycotoxin contamination of stored rice grains, and microbe-based (biological) strategies to control grain fungi and mycotoxins. The latter will include information regarding attempts undertaken for mycotoxin (especially aflatoxin) bio-detoxification and microbial interference with the aflatoxin-biosynthetic pathway in the toxin-producing fungi.

Mycoflora and Mycotoxins of Cereal Grains in Delta, Egypt

  • Soliman, Hoda M.
    • Mycobiology
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    • v.31 no.4
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    • pp.183-190
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    • 2003
  • Five cereal grains(wheat, barley, rice, maize and sorghum) were collected from three Egyptian provinces known to be grain producers(Daqahlia, Gharbia and Kafer el-Sheikh). Two species of Alternaria(A. raphani and A. tenusinae); two species of Aspergillus(A. flavus and A. niger); one species of Cunninghamella(C. elegans); one Dreschslera species(D. myakt); three Fusarium species(F. graminearum, F. moniliform and F. solani); one Rhizopus species(R. stolonifer) and two species of Penicillium(P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. All the fungal isolates were tested for the production of toxic metabolites in culture media and the percentages of toxigenic isolates were calculated. The biological assay of the toxigenic fungal isolates showed significant variations in toxigenic activity. Thin layer chromatography revealed that the most active isolate produces moniliformin in culture media. The effect of culture conditions on the production of moniliformin was studied.

Ecological and Physiological Studies on Soil Fungi at Western Region, Libya

  • El-Said, A.H.M.;Saleem, A.
    • Mycobiology
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    • v.36 no.1
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    • pp.1-9
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    • 2008
  • Sixty three species and 5 varieties belonging to 30 fungal genera were collected from 75 soil samples. Cultivated (29 genera and 58 species + 5 var.), desert (22 and 35 + 2 var.) and saline soil (21 and 41 + 1 var.) fungi were recovered on glucose-, cellulose- and 50% sucrose-Czapek's agar at $28^{\circ}C$. The most common genera were Alternaria, Aspergillus, Emerieella, Fusarium, Mycosphaerella, Nectria and Penicillium. The most prevalent species from the three types of soils on the three types of media were Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emerieella nidulans, Fusarium oxysporum, Myeosphaerella tassiana, Nectria haematococca and Penicillium ehrysogenum. Chaetomium globosum was in the top of fungi in producing endo-$\beta$-1,4-glucanases among the 42 tested isolates obtained from soils on cellulose-Czapek's agar. Maximum production of this enzyme by C. globosum obtained after 6 days of incubation at $30^{\circ}C$ with culture medium containing maltose as a carbon source and ammonium nitrate as a nitrogen source and pH initially adjusted to 6.

Synthesis and Characterization of Transition Metal Complexes with Benzimidazolyl-2-hydrazones of o-anisaldehyde and Furfural

  • Mohapatra, R.K.;Mishra, U.K.;Mishra, S.K.;Mahapatra, A.;Dash, D.C.
    • Journal of the Korean Chemical Society
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    • v.55 no.6
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    • pp.926-931
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    • 2011
  • A series of complexes of the type [$ML_2Cl_2$], where L=2-(o-anisylidene-2'-imino) amino benzimidazole (AIAB) and 2-(furfurylidene imino) amino benzimidazole (FIAB), M=Cu(II), Co(II), Ni(II) and Zn(II), have been synthesized and characterized on the basis of elemental analysis, thermal analysis, molar conductivity, magnetic moment, electronic, infrared, $^1H$-NMR spectral studies. The results are in consistent with bidentate chelation of ligand with azomethine nitrogen and ring nitrogen donors. All these Schiff bases and their complexes have also been screened for their antibacterial (Bacillus subtilis, Bacillus stearothermophilus, Escherichia coli and Salmonella typhi) and antifungal activities (Aspergillus niger and Aspergillus flavus).