• YAKHAK HOEJI
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• v.36 no.5
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• pp.469-473
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• 1992

Yeast alcohol dehydrogenase (YADH) has an acidic residue that interacts with the 2'- and 3'-hydroxyl groups of the adenosine ribose of the $NAD^+$ coenzyme. The acidic residue of Asp-223 (according to horse liver alcohol dehydrogenase amino acid sequence) is supposed to determine the coenzyme specificity for $NAD^+$ rather than $NADP^+$. We mutated Asp-223 to leucine and the mutant YADH was expressed in yeast and characterized for the coenzyme specificity. The turnover numbers of mutant enzyme for $NAD^+$ and ethanol were decreased 3.5- and 4.8-fold compared to wild-type enzyme, respectively. Contrastively, catalytic specificity for $NADP^+$ was increased 13-fold. As a result, the mutant YADH also employed $NADP^+$ as a coenzyme.

• 한국디지털정책학회:학술대회논문집
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• 2006.12a
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• pp.223-235
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• 2006

2006년 3/4분기 현재 국내 300만 사업체 중 70만개 업체가 ASP 서비스를 이용하며, ASP 서비스를 제공하는 사업체 수는 159개 인 것으로 조사되었다. 국내 159개 ASP 서비스 제공업체를 대상으로 BSI를 조사해 본 결과 실적치는 고객 수(108.5)보다 매출액(106.6), 매출액보다는 경상이익(98.1)에 대한 경기를 보다 어둡게 보는 것으로 나타났다. 따라서 BSI를 통해 본 ASP산업은 외형적으로는 국내 70만개라는 사업체가 ASP를 통해 각종 IT솔루션을 이용하고 있는 반면에 ASP 서비스를 제공하는 ASP 제공업체의 경영 채산성은 상대적으로 열악함을 의미하고 있다. 따라서 향후 외국 대형 소프트웨어 유통업체들의 국내 진입 선언 등과 더불어 국내 ASP 제공업체들의 대응 전략 마련이 절실히 필요로 하는 때임을 암시하고 있다.

• Microbiology and Biotechnology Letters
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• v.7 no.4
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• pp.217-223
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• 1979

Six strains of mold which had high saccharifying and acid-productive ability were isolated from wild sources. The strain M-80 among them and Aspergillus kawachii, which was generally used as rice makkulli koji were used for this studies. The results obtained were summarized as follows. 1) $\alpha$-amylase activities of the strain M-80 and Asp, kawachii showed similarly as about 140W. V., while $\beta$-amylase activity of M-80 was 54 A. U. and Asp. kawachii was 40 A. U. 2) Acid protease activity of M-80 was higher then Asp. kawachii but alkaline protease activity was lower Asp. kawachii respectively. 3) The contents of total acid, ethanol and fusel oil in makkulli brewing with M-80 were higher those of Asp. kawachii and methanol contents of the tested two strains were about 33mg/％. 4) Fifteen kinds of free amino acid were detected from makkulli brewing of two strains, and free amino acid contents of M-80 were 10％ higher than those of Asp. kawachii.

• The Korean Journal of Food And Nutrition
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• v.33 no.3
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• pp.223-236
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• 2020

In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

• Proceedings of the Korea Association of Information Systems Conference
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• 2000.05a
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• pp.223-228
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• 2000

웹사이트는 정보 미디어와 애플리케이션 플랫폼을 가지고 널리 사용되어지고 있다. 작은 기업들이 큰 기업들과 경쟁하기 위해서 웹사이트 구축과 웹에 기반한 애플리케이션 개발이 절실히 요구되어지고 있다. 하지만, 대부분의 웹사이트 구축과 웹 애플리케이션의 생성은 체계적인 프로세스 없이 개발자들의 지식과 경험에 의존하여 임시직으로 개발되어 왔다. 따라서, 이러한 웹사이트 구축과 웹 애플리케이션 생성에 체계적인 프로세스를 가지는 소프트웨어 공학이 적용되어 개발, 유지되어져야 한다. 본 논문에서는 최근에 ActiveX, Java, ASP, CORBA등 다양한 형태의 코드와 플러그-인으로 개발되는 웹사이트와 웹 응용 애플리케이션에서 컴포넌트의 형태로 요소들을 구성, 접근하여 사용하고, 다른 웹사이트 구축이나 다른 웹 애플리케이션에서 이미 사용한 구성 성분을 재사용 함으로써 새로운 웹사이트 구축과 웹 애플리케이션 개발이 가능한 성분조립모델(the Component Assembly Model)을 웹사이트 개발 프로세스로써 활용하고자 한다.

• KSBB Journal
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• v.16 no.2
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• pp.188-199
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• 2001

A gene encoding the dextransucrase(dsCB) that synthesizes mostly $\alpha-(1\rightarrow6)$ linked dextran with low amount(10%) of $\alpha-(1\rightarrow3)$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1 kbp DNA fragment carrying dsCB showed one open reading frame(ORF) composed of 4,536bp. The deduced amino acid sequence shows that it begins from the start codon(ATG) at position 698 of the cloned DNA fragment and extends to the termination condon(TAA) at position 5,223. The enzyme is consisted of 1,508 amino acids and has an calculated molecular mass of 168.6kDa. This calculated Mw was in good agreement with an activity band of 170kDa on non-denaturing SDS-PAGE. A recombinant E. coli DH5 $alpha$ harboring pDSCB produced extracellular dextransucrase in 2% sucrose medium, and synthesized both soluble and insoluble dextran. To compare the properties of enzyme with B-742CB dextransucrase, the acceptor reaction, hydrolysis of dextran and methylation were performed. The expressed enzyme showed the same properties as B-742CB dextransucrease, but its ability to synthesize $\alpha-(1\rightarrow3)$ branching was lower than that of B-742CB dextransucrase. In order to identify the critical amino acid residues known as conserved regions related to catalytic activity, Asp-492 was replaced with Asn. D492N resulted in a 1.6 fold decrease in specific activity.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2007.02a
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• pp.213-223
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• 2007

E-Business Platform for SMEs in GMS Countries(GMSEB) is a E-business service portal which based on Yunnan, face to ASEAN, connect with China's mainland. lt is established by Kunming Dongxun Sic&Tech Co.,Ltd and E-Business Centre of Yunnan University of Financial & Economics, who get supports from UNESCAP, Science and Technology Department of Yunnan Provincial and Yunnan SMEs Bureau. The portal will bulid a B2B & B2C E-business and. ASP portal to provide service of e-business and enterprise management informatization according with the different languages and situation to China and GMS countries. The paper starts with analysis of the policy in China of promoting GMS cooperation and exchanges, importance of Yunnan and cultural universality in GMS countries. It expounds the necessity of establishing such a regional e-commerce platform. The project explores how to develop the model of e-business and enterprise management informationization for SMEs under the condition of weak economic strength and scarcity of Information Technology talents. To promote the SMEs in GMS countries participate in the integration with the global economy through the international SMEs'E-business Platform, make a fairly rapid progress by full play to its advantages.

• Journal of Genetic Medicine
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• v.19 no.1
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• pp.27-31
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• 2022

X-linked dominant hypophosphatemic rickets are the most common form of familial hypophosphatemic rickets resulting from hypophosphatemia caused by renal phosphate wasting, which in turn is a result of loss-of-function mutations in PHEX. Herein, we report a 39-year-old female with short stature and skeletal deformities and 12-month-old asymptomatic daughter. The female has a history of multiple surgical treatments because of lower limb deformities. Her biochemical findings revealed low serum phosphorus levels with elevated serum alkaline phosphatase activity and normal serum calcium levels, suggesting presence of hypophosphatemic rickets. To identify the molecular causes, we used a multigene testing panel and found a mutation, c.667dup (p.Asp223GlyfsTer15), in PHEX gene. To the best of our knowledge, this is a novel mutation. A heterozygous form of the same variant was detected in daughter, who showed no typical symptoms such as bow legs, frontal bossing, or waddling gate, but presented early signs of impaired mineralization in both X-ray and biochemical findings. The daughter was initiated onto early medical treatment with oral phosphate supplementation and an active vitamin D analog. Because the daughter was genetically diagnosed based on a family history before the onset of symptoms, appropriate medical management was possible from early infancy.