• Title/Summary/Keyword: Ascorbate oxidase(AO)

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Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media (유기 용매 속에서 L-Ascorbic Acid의 정량을 위한 바이오센서)

  • Kwon, Hyo-Shik;Lee, Cheal-Gyu
    • Analytical Science and Technology
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    • v.7 no.2
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    • pp.149-153
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    • 1994
  • An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was $2{\times}10^{-6}M$ L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.

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Effects of Light on Activities of Antioxidative Enzymes in Hairy Root Cultures of phytolacca esculenta Houtte (자리공(Phytolacca esculenta van Houtte) 모상근배양에서 항산화효소의 활성에 미치는 광의 영향)

  • 양덕조;김용해;권진이;최철희;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.2
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    • pp.71-76
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    • 1995
  • The effects of light on the activities of several antioxidative enzymes, catalase (CAT), superoxide dismutase(SOD), ascorbate oxidase(AO), and peroxidase(POD) were examined in the hairy root cultures of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Activities of CAT, SOD, and AO were significantly decreased with incresing light intensity (500-2,000 lx). The activity of AO under high light condition (2,000 lx)was decreased by 92% compared to the dark condition. The activities of glutathoine peroxidase (GPO), ascorbate peroxidase (APO) and general POD were increased under lower light intensify below 500 lx. The activity of GPO under 2,000 lx was decreased by 85% compared to the dark condition. The activities of antioxidative enzymes were more decreased in blue light (400-500nm). The activities of antioxidative enzymes in blue light intensity were increased in lower light intensity below 30 lx, but decreased 21-70% under 200 lx. The activity of AO was decreased by 70% under 200 lx with increasing blue light intensity. Our results suggest that the activities of antioxidative enzymes in hairy roots might be inhibited by endogenous oxidants generated under the high blue light conditions.

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