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Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste

  • Triyaswati, Desty;Ilmi, Miftahul
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.167-178
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    • 2020
  • Lipase-producing fungi have been isolated from environments containing lipids. The non-dairy creamer industrial waste has a high amount of lipids so it is a potential source for the isolation of lipase-producing fungi. However, the study of fungi that secrete lipase from this industrial waste has not been reported. The purpose of this study was to obtain lipase-producing filamentous fungi from non-dairy creamer industrial waste. Mineral salt and potato dextrose agar were used as media for the isolation process. The qualitative screening was conducted using phenol red agar medium and the quantitative screening using broth medium containing glucose and olive oil. Isolates producing the highest amounts of lipase were identified with molecular methods. We found that 5 out of 19 isolated filamentous fungi are lipase producers. Further analysis showed that isolate Ms.11 produced the highest amount of lipase compared to others. Based on ITS sequence Ms.11 was identified as Aspergillus aculeatus. The lipase activity in medium containing 1% glucose + 1% olive oil at pH 7.0 and 30℃ after 96 and 120 h of incubation was 5.13 ± 0.30 U/ml and 5.22 ± 0.59 U/ml, respectively. The optimum lipase activity was found at pH 7.0, 30℃ and using methanol or ethanol in the reaction tube. Lipase was more stable at 20-30℃ and maintained 85% of its activity. It was concluded that isolate Ms.11 is a potential source of lipase that catalyzes transesterification reactions. Further studies are required to optimize lipase production to make the strain suitable for industry purposes.

The Analysis for Legal Evolution of Affirmative Action in University Admissions in the U. S. A. (대학입학과 관련된 미국 소수집단우대정책 역사적 변화 분석)

  • Lim, Soojin
    • Korean Journal of Comparative Education
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    • v.22 no.4
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    • pp.149-178
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    • 2012
  • The initial purpose of Affirmative Action(AA) in the U. S. was to increase access to, and ensure the equitable distribution of, opportunities for racial minority groups in order to redress past discrimination. Over the last several decades, support for AA has grown and waned as structural, political and social currents have shifted. Most recently, AA in university admissions policy was once again tested as The University of Texas at Austin successfully defended its use of AA in admissions and now faces Supreme Court review concerning the lawsuit. Fisher v. University of Texas at Austin. The purpose of this study is to illustrate the evolution of AA in university admissions reflected on major legal cases for and against it. AA is analyzed from the integrative approach based on the historical institutionalism. l.e., influenced by structure, political dynamics, institutions and critical actors.

Fabrication of Strain Sensor Based on Graphene/Polyurethane Nanoweb and Respiration Measurement (그래핀/폴리우레탄 나노웹 기반의 스트레인센서 제작 및 호흡측정)

  • Lee, Hyocheol;Cho, Hyeon-seon;Lee, Eugene;Jang, Eunji;Cho, Gilsoo
    • Science of Emotion and Sensibility
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    • v.22 no.1
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    • pp.15-22
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    • 2019
  • The purpose of this study is to develop a strain sensor based on a nanoweb by applying electrical conductivity to a polyurethane nanoweb through the use of Graphene. For this purpose, 1% Graphene ink was pour-coated on a polyurethane nanoweb and post-treated with PDMS (Polydimethylsiloxane) to complete a wearable strain sensor. The surface characteristics of the specimens were evaluated using a field emission scanning electron microscope (FE-SEM) to check whether the conductive material was well coated on the surface of the specimen. Electrical properties of the specimens were measured by using a multimeter to measure the linear resistance of the specimen and comparing how the line resistance changes when 5% and 10% of the specimens are tensioned, respectively. In order to evaluate the performance of the specimen, the gauge factor was obtained. The evaluation of the clothing was performed by attaching the completed strain sensor to the dummy and measuring the respiration signal according to the tension using MP150 (Biopac system Inc., USA) and Acqknowledge (ver. 4.2, Biopac system Inc., U.S.A.). As a result of the evaluation of the surface characteristics, it was confirmed that all the conductive nanoweb specimen were uniformly coated with the Graphen ink. As a result of measuring the resistance value according to the tensile strength, the specimen G, which was treated with just graphene had the lowest resistance value, the specimen G-H had the highest resistance value, and the change of the line resistance value of the specimen G and the specimen G-H is increased to 5% It is found that it increases steadily. Unlike the resistance value results, specimen G showed a higher gauge rate than specimen G-H. As a result of evaluation of the actual clothes, the strain sensor made using the specimen G-H measured the stable peak value and obtained a signal of good quality. Therefore, we confirmed that the polyurethane nanoweb treated with Graphene ink plays a role as a breathing sensor.

The Effect of $\textrm{WO}_3$, on the Microstructure and Electrical Properties of ZNR (ZNR의 미세구조 및 전기적 특성에 $\textrm{WO}_3$가 미치는 영향)

  • Nam, Chun-U;Jeong, Sun-Cheol;Park, Chun-Hyeon
    • Korean Journal of Materials Research
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    • v.9 no.7
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    • pp.753-759
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    • 1999
  • The microstructure and electrical properties of ZNR that W $O_3$ is added in the range 0.5~4.0mol%, were investigated. The major part of W $O_3$ were segregated at the nodal point and W-rich phase was formed. Three crystalline phases, such as W-rich phase (W $O_3$), Bi-rich phase (B $i_2$W $O_{6}$ ), and spinel phase (Z $n_{2.33}$S $b_{0.67}$ $O_4$) were confirmed to be co-existed at the nodal point The average grain size increased in the range 15.5~29.9$\mu\textrm{m}$ with increasing W $O_3$ additive content. Consequently. W $O_3$ acted as a promotion additive of grain growth. As the W $O_3$ additive content increases. the varistor voltage and the nonlinear exponent decreased in the range 186.82~35.87V/mm and 20.90~3.34, respectively, and the leakage current increased in the range of 22.39~83.01 uh. With increasing W $O_3$ additive content, the barrier height and the density of interface states decreased in the range 1.93~0.43eV and (4.38~1.22)$\times$10$^{12}$ $\textrm{cm}^2$, respectively. W $O_3$ acted as an acceptor additive due to the donor concentration increasing in the range (1.06~0.38)$\times$10$^{18}$ /㎤with increasing W $O_3$ additive content.t.t.

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The Literature Review on Medications of Insomnia in Chapter Mong(夢) of Donguibogam (불면 처방에 관한 문헌고찰 - 동의보감(東醫寶鑑) 몽문(夢門)을 중심으로)

  • Park, Bo-Ra;Park, In-Sook;Kang, Hyung-Won;Lyu, Yeong-Su
    • Journal of Oriental Neuropsychiatry
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    • v.22 no.2
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    • pp.177-198
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    • 2011
  • Objectives : By analyzing and comparing indications, components and their doses of herb medicines, which were recorded in insomnia chapters of medical books, we examined historical changes and made clear documentary ground of medications. Methods : 1. 10 books which were noted in insomnia part of "The Eastern Medical Textbook of neuropsychiatry" and 13 books were mentioned in same part of "Uibujeonrok" were selected as reference. 2. We searched article database such as KISS, riss4U and journals issued by the Korean society of oriental neuropsychiatry. Finally, 39 books which were noted in 3 literature reviews, were chose as reference. 3. In reference books, we collected the herb medicines in chapters related to insomnia. 4. In case of no section associated with insomnia in books, we found prescriptions related to insomnia in whole chapters. 5. If the chief virtue of medication was not primarily for insomnia, we excluded applied or added herb medicines. 6. We classified prescriptions into small groups which had same name, and then rearranged them in chronological order. Results and Conclusions : 1. Banha-tang(banxiatang) of "Hwangjenaegyeong Youngchu" was the first-recorded prescription used for insomnia. 2. The chapter of insomnia appeared in "Chunkeumyobang" at first. 3. Ondam-tang(Wendantang) was most cited prescription in medical books. It was recorded totally 19 books from "Chunkeumyobang" to "Hyeoljeungron". 4. We suspects indications, components, dosage of medication of insomnia in Donguibogam were revised according to author's clinical experience and circumstances.

Salivary Levels of ErbB2 and CEA in Oral Squamous Cell Carcinoma Patients

  • Honarmand, Marieh;Farhad-Mollashahi, Leila;Nakhaee, Alireza;Nehi, Masoume
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.sup3
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    • pp.77-80
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    • 2016
  • Oral squamous cell carcinoma (OSCC) is one of the ten most common causes of cancer death worldwide. Assessment of molecular changes can help detect and control lesions. The aim of this study was evaluation of salivary levels of ErbB2 and CEA in OSCC patients. In this case-control study, 27 OSCC patients and 26 healthy controls participated. After obtaining consent and filling out a questionnaire, unstimulated saliva samples were collected from people in the morning for measurement of the two markers using ELISA. Data were analyzed using Mann Whitney U test in SPSS 19 software with P<0.05 considered significant. Subjects were in the age range of 40 to 65 years. \Salivary level CEA was elevated in OSCC samples ($42.6{\pm}21.1ng/ml$) as compared to those of controls ($22.6{\pm}22.1ng/ml$) (p< 0.01), but no significant variation was noted for ErbB2 ($5.2{\pm}1.8ng/ml$ and $4.93{\pm}2.14ng/ml$, p=0.28). The role of ErbB2 as a tumor marker in patients with OSCC must still be regarded as controversial and needs further studies to clarify any significance for early detection or screening. In contrast the salivary level of CEA may find application for early detection of patients.

Studies on the In Vitro Fertilization and In Vitro Development of Porcine Embryos (돼지 난포란의 체외수정 및 체외발달에 관한 연구)

  • 박성원;홍승표;진종인;이지삼;정장용;박회성
    • Journal of Embryo Transfer
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    • v.14 no.3
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    • pp.185-193
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    • 1999
  • To improve the efficiency of in vitro production of embryos with follicular oocytes in pig, the recovery rates, in vitro fertilization and development. The results obtained were as fellows: The number of oocytes recovered 37 ovary was 1,365 by aspiration, 1,884 by slicing and 3,830 aspiration post slicing, per ovary was averaged 103.5 aspiration post slicing than 30.7 by aspiration and 50.8 by slicing (P<0.05). The percentage of grade I and II oocytes recovered was 0.05∼0.2% and 1.7∼2.3% respectively(p<0.05). The fertilization rates of ejaculate and epididymis sperm was 83.0 and 83.1%. And cleavaged rate was 60.8 and 69.0% respectively(P<0.05). However, there were no significant differences between sperm sources. The clevage rates of fertilized oocyte was significantly(P<0.05) higher as B.O(92.8%) than TALP (90.1%) or mTBM (80.1%). And in vitro developed to blastocyst rates of mTBM media used for fertilization was significantly (P<0.05) higher as 12.4%, compared with the results using the media of TALP(1.6%) or B.O (0.0%). The embryos developed 2-cell stage after in vitro fertilization were co-cultured with or without POEC and BOEC in NCSU-23 and TCM-199 media. In vitro developed to blastocyst rates was NCSU-23 with POEC(2.3%) or BOEC(1.2%), but in vitro cultured in TCM-199 medium with POEC or BOEC was not developed to blastocyst. The percentage of embryos that developed to morula stage in 0, 50, 100, 200 and 250uM was 16.6, 22.0, 13.5, 19.0 and 22.0%, respectively.

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An Experimental Study on Effect on Debonding Strength of Metal-porcelain by Painting Method of Opaque Porcelain (Opaque 도재의 도포 방법에 따른 금속-도재의 결합강도에 미치는 영향에 관한 실험적 연구)

  • Kim, Sa-Hak
    • Journal of Technologic Dentistry
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    • v.22 no.1
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    • pp.39-47
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    • 2000
  • This study is the know the difference bond strength according to painting method of Opaque porcelain. Among Porcelain powder for Porcelain, Noritake powder, Bonding agent 1 class and metal are selected as Base metal alloy. And painting method of Opaque porcelain is divided by 4 groups : PG, WG, NG and DG. Also it is to manufacture 24 sheets of Specimen by 6 each every method and to measure Crack-initiation Strength of Metal-porcelain and it is to observe it by Scanning electron microscope. I come to get a conclusion as follows. 1. As for Debonding strength of every experiment groups, it showed that WS group which executed Wash bake had highest value, 44.25MPa and NG group which used Normal powder had value, 42.11MPa and DG group which used Bonding agent had value, 35.88MPa and PG group which used Paste opaque had value, 35.39MPa. 2. In four painting methods of Opaque porcelain, PG group, WG group, NG group and DG group, there is no significant difference statistically in Crack-initiation Strength. 3. As a result of observing fracture surface with Scanning electron microscope, it was showde that PG group remained a lot of particle of porcelain on the surface of metal than WG group, NG group and DG group.

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The magnetic characteristics of Co-Cr-(Ta) films (Co-Cr-(Ta)박막의 자기특성)

  • Kim, K-H;Jang, K-U;Kim, J-H;S Nakagawa;M Naoe
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1996.11a
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    • pp.242-244
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    • 1996
  • The effects of $Co_{67}$C $r_{33}$ underlayer on the crystallographec and magnetic characteristics of the Co-Cr-Ta layer deposited on the underlayer was investigated. The diffraction intensity $I_{p(002)}$ of Co-Cr-Ta layers on the $Co_{67}$C $r_{33}$ layer was stronger than that of single layer and Co-Cr-Ta/Ti double layer. Therefore, the crystallinity of Co-Cr-Ta layer was improved by the $Co_{67}$C $r_{33}$ underlayers rather than Ti ones. However, the coercivity $H_{c}$ of Co-Cr-Ta layers deposited on $Co_{67}$C $r_{33}$ underlayer was as low as 250 Oe even at substrate temperature of 22$0^{\circ}C$. This $H_{c}$ decrease seems to be attributed to the effect of the $Co_{67}$C $r_{33}$ underlayer as well as interval time between deposition of the underlayer and the Co-Cr-Ta layer.yer.layer.yer.

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The Effect of Noise and Background Music on the Trunk Muscle Fatigue during Dynamic Lifting and Lowering Tasks (들기/내리기 작업 시 소음과 배경음악이 몸통근육 피로도에 미치는 영향)

  • Kim, Jung-Yong;Shin, Hyun-Joo;Lee, In-Jae
    • Journal of the Ergonomics Society of Korea
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    • v.27 no.3
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    • pp.15-22
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    • 2008
  • The purpose of this study was to define the effects of noise and background music on the trunk muscle fatigue during dynamic lifting and lowering tasks. Six healthy male subjects with no prior history of low back disorders participated in this study. The participants were exposed to two levels of background noise such as 40dB noise and 90dB noise and three levels of background music such as no music, slow music, and fast music. Six different combinations of background noise and background music were played while the participants were performing the lifting task at 15% level of Maximum Voluntary Contraction. Electromyography signals from six muscles were collected and fatigue levels were analyzed quantitatively. In results, the 90dB noise increased trunk muscle fatigue and slowed down the recovery. The trunk muscle fatigue was the lowest when the fast music was played for as background. After recovery, the 90dB noise increased trunk muscle fatigue. The trunk muscle fatigue was the lowest when the slow music was played for as background. The results can be useful to manage the cumulative fatigue of trunk muscles due to background noise and music during repetitive lifting and lowering tasks in industry.