• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.207.1-207
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• 1997

No Abstract, See Full Text

• Animal cells and systems
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• v.11 no.2
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• pp.175-182
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• 2007

The lysozyme II gene of cabbage butterfly Artogeia rapae was cloned from fat body of the larvae injected with E. coli and its nucleotide sequence was determined by the RACE-PCR. It has an open reading frame of 414 bp nucleotides corresponding to 138 amino acids including a signal sequence of 18 amino acids. The estimated molecular weight and the isoelectric point of the lysozyme II without the signal peptide were 13,649.38 Da and 9.11, respectively. The A. rapae lysozyme II (ARL II) showed the highest identity (81%) in the amino acid sequence to Manduca sexta lysozyme among other lepidopteran species. The two catalytic residues ($Glu^{32}$ and $Asp^{50}$) and the eight Cys residue motifs, which are highly conserved among other c-type lysozymes in invertebrates and vertebrates, are also completely conserved. A phylogenetic analysis based on amino acid sequences indicated that the ARL II was more closely related to M. sexta, Hyphantria cunea, Heliothis virescens, and Trichoplusia ni lysozymes. The ARL II gene was expressed in Spodoptera frugiperda 21 insect cells and the recombinant ARL II (rARL II) was purified from cell-conditioned media by cation exchange column chromatography and reverse phase FPLC. The purified rARL II was able to form a clear zone in lysoplate assay against Micrococcus luteus. The lytic activity was estimated to be 511.41 U/mg, 1.53 times higher than that of the chicken lysozyme. The optimum temperature for the lytic activity of the rARL II was $50^{\circ}C$, the temperature dependency of the absolute lytic activity of rARL II was higher than that of the chicken lysozyme at low temperatures under $65^{\circ}C$.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.401-405
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• 2008

This study was conducted to estimate the control thresholds (CTs) of imported cabbage worm, Artogeia rapae L., injuring Chinese cabbage. The second instar larvae of A. rapae were inoculated with five density levels on each Chinese cabbages transplanted three weeks earlier under greenhouse condition, and checked injury rates after allowing their feeding for one week and two weeks, respectively. The average leaf area consumed by single larvae was 657.7 $mm^2$ in plots inoculated at three weeks after transplanting (WAT) and 2495.8 $mm^2$ in plots at 6-WAT, respectively. In the field experiment, different numbers of A. rapae ranged from one to seven larvae were inoculated on 20 plants. The percent yield reduction (Y) of Chinese cabbage infested by different densities of A. rapae (X) for a three-week period was estimated by the following equation; (1) Y=1.764X-0.3049 ($R^2$=0.9901) in plots inoculated at 3-WAT; and (2) Y=1.0305X-0.2976 ($R^2$=0.9398) in plots inoculated at 6-WAT. Based on the relationships between the densities of A. rapae larvae and the yield index of Chinese cabbage, the number of second instar larvae which caused 5% loss of yield (gain threshold proposed by Japan), was estimated as 3.0 per 20 plants for the 3-WAT and 5.1 for the 6-WAT.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.253.2-253
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• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.253.1-253
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• 1998

No Abstract, See Full Text

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.114-114
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• 2002

• Korean journal of applied entomology
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• v.42 no.1
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• pp.21-27
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• 2003

This study was carried out to investigate the changes of insects diversity and host plants from 1998 to 2001 after the construction of an insect park in Suwon in Korea. After the construction in 1997 the insect species and populations have been increased yearly to date. A total of 343 species out of 138 families of 11 orders was surveyed at the park from April to November in 2001. The number of species increased from April to July and showed a peak in June. Coleoptera was a dominant order, and Dolichus halensis (Schaller) of Coleoptera, Artogeia rapae (Linne) of Lepidoptera, and Liorhyssus hyaalinus (Fabricius) of Hemiptera were dominant species.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.348-355
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• 1995

This Investigation was carried out to map the morphological development of serotonin-immunoreactive neurons in the larval brain of the cabbage butterfly, Artogeia rapae, during five larval stages. Both the first instar larva and the second instar larva contained twenty serotonin-immunoreactive (5-HTi) neurons in each brain. The fibres of 5-HTI commissure was interconnected to two cerebral hemispheres in both brains. However, the 5-HTi commissural fibres was Increased in number in the second-instar larva brain. In the brain of the second Insar larva these 5-HTi fibres formed rich arborization in contralateral neuropils, especially In the posterior parts of it. The third-Instar larva braIn, which Included twenty two 5-HTi neurons, had three groups of 5-HTi commissural fibres. In the fourth Instar larva, the number of 5-HTi fibres as well as 5-HTi cell bodies increased in the brain. The fifth-instar larva brain, which contained fifty four 5-HTi cell bodies, showed the largest number of 5-HTi cell bodies In developing larval brains. The 5-HTi fibres formed richest commissural fibres and some of them run parallel to anteroposterior axis.

• Asian Journal of Turfgrass Science
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• v.18 no.4
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• pp.221-229
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• 2004

Biological control of lepidopterous insect pests, Agrotis segetum, Artogeia rapae, Mamestra brassicae, Plutella xylostella, Spodoptera exigua, and S. litura with entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae isolated from Gyeongbuk province were investigated. Mean lethal concentration ($LC_50$) values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of A. segetum larvae were $1.2\times10^7\;conidia/m\ell$ and $5.2\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of A, rapae larvae were $1.2\times10^7\;conidia/m\ell$ and $5.2\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of M. brassicae and P. xylostella, larvae were $1.5\times10^6\;conidia/m\ell$, $9.7\times10^5\;conidia/m\ell$, $3.0\times10^6\;conidia/m\ell$ and $1.4\times10^6\;conidia/m\ell$, respectively. $LC_50$ values of B. bassiana and M. anisopliae against $2\cdot3rd$ instar of S. exigua, and S. litura larvae were $6.3\times10^6 \;conidia/m\ell$, $2.6\times10^6\;conidia/m\ell$, $1.6\times10^7\;conidia/m\ell$ and $3.4\times10^6\;conidia/m\ell$ respectively.

• Korean Journal of Environment and Ecology
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• v.10 no.2
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• pp.171-183
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• 1997

Diversity, abundance, species distributions and distributions of two strategists (specialist and geceralist) of butterflies were investigated monthly from June 1996 to Oct. 1996 by line transect method in the Chunbul-dong valley from park gate to Yangpok Sanjang. The butterfly faunas of the eight regions in Kwangwon province, Gyunggi proavince and Pusan were also studied by the same method to assess diversity and abundance of butterfly community in the study region. The 18 butterfly species in 7 families were found in this study and the 48 butterfly species in 7 families were distributed in the study area from thes study and the other researches. The 30 butterfly species reported in the other studies were not found in this study and Kaniska canace L. of them would be to decrease in density. In the study area, the common species were Artogeia melete M., Neptis sappho P. and Libythea celtis F.. The representative Korean common butterflies such as Artogeia rapae L., Colias erate E. and Papillio xuthus L. were rare. The PCA(Principal Component Analysis) ordinations indicate that habitat disturbance by human would be major factor for butterfly community and species distribution. Difference was not found in diversity according to habitat disturbance. Abundance, however, was higher in the disturbed region than the undisturbed region. Compared with the other regions, abundance was greatly lower and diversity was slightly lower than that of the other regions. Two strategists exhibited different distribution patterns. Generalists were found more commonly in the disturbed region and widely distributed in both of the disturbed region and the undisturbed region than specialists.