• Title/Summary/Keyword: Artificial substrate

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Evaluation of the Useful Bioactivities of Spent Mushroom Substrate of Shiitake (표고버섯 수확 후 배지의 유용 생리활성 평가)

  • Sung, Hwa-Jung;Pyo, Su-Jin;Park, Jong-Yi;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.29 no.2
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    • pp.164-172
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    • 2019
  • In Korea, shiitake mushroom, Lentinula edodes, is cultivated on artificial medium containing oak sawdust and wheat bran. The annual production of spent mushroom substrate (SMS) of shiitake, a byproduct of the mushroom industry, is estimated to reach over 50,000 tons per year. This study aimed to improve the use of SMS as a novel bioresource. Hot water extracts of SMS after the first and third harvest were prepared and their bioactivities evaluated. Hot water extracts of uninoculated medium and shiitake were used as controls. Extracts of SMS showed higher radical scavenging of DPPH anions, ABTS cations, nitrites, and a higher reducing power than those of shiitake or medium extracts. After the first and third harvests at 0.5 mg/disc, SMS extracts showed no antibacterial or antifungal activities against the pathogenic and food-spoilage bacteria and fungi. However, they showed good inhibitory activities against ${\alpha}$-glucosidase at 0.5 mg/ml. In addition, SMS extracts had strong anti-coagulation activities via their inhibition of thrombin, prothrombin, and blood coagulation factors without platelet aggregation activity. Our results suggested SMS should no longer be perceived as a useless byproduct but should be understood as a novel bioresource, the extracts of which could be developed as antioxidant, antidiabetic, and antithrombosis agents.

Effects of Soil Depth and Irrigation Period on Some of the Native Plants in and Artificial Substrate of Roof Garden (옥상녹화용 인공배합토에서 토심 및 관수주기에 따른 몇몇 자생식물의 생육특성)

  • Bang, Kwang-Ja;Ju, Jin-Hee;Kim, Sun-Hae
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.6
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    • pp.75-83
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    • 2004
  • Focusing on native plants that have high possibility of being introduced as rooftop material, this study was conducted to investigate extensive and easy-to-manage rooftop garden and to raise the utilization of native plants by verifying their growing response to artificial substrate soil depth and irrigation period. The study was conducted from March to September in 2002. Plants tested included Chrysanthemum zawadskii, Sedium middendorffianum, Thymus quinquecostatus, Allium senescens, and Dianthus superbus. Regarding soil depth, it was 5 cm and 10 cm. Irrigation period was non-irrigation, 1-week, 2-weeks, and 3- weeks, Its result is as follows; 1. In case of Sedum middendorffianum Maxim, mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. 2. In case of Allium senescens L., mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. Therefore, Provided that fertilizing is managed well, it is a plant that can be highly utilized.3. In case of Chrysanthemum zawadskii Herb. Subsp. (Nakai) Y. Lee Stat., the growth of top was lower in 10cm than in 5cm and it grew well in 10cm. When utilizing for rooftop garden, it would be desirable to keep minimum viable soil depth at over 10cm. If there is enough rainfall, soil and soil depth seem to have greater effect on growth than irrigation period does. 4. In case of Diauthus superbus L. var. longicalycinus (Maxim) Williams, rooting rate and growth were better in 10cm than in 5cm. Therefore, it is desirable to keep minimum soil depth at over 10cm. 5. In case of Thymus quinquecostatus Celak, the growth of top and flowering were better in 10cm than in 5cm. Therefore, it seems desirable to have minimum viable soil depth to be over 10cm. In conclusion, the most suitable species for rooftop garden are Sedium middendorffianum and Allium senescens in this experiment. However, Chrysanthemum zwadskii, Thymus quinquecostatus, and Dianthus chinensis also can be utilized greatly when irrigation is managed regularly in artificial mixed soil over 10cm.

Environmental Studies in the Lower Part of the Han River - IX. Succession of Epilithic Diatoms on the Artificial Substrate and Factor Analysis (한강하류의 환경학적연구 - IX. 인공기질을 이용한 부착돌말류의 천이와 요인분석)

  • Park, Sung-Hwan;Lee, Jin-Hwan
    • Korean Journal of Environmental Biology
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    • v.25 no.1
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    • pp.16-26
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    • 2007
  • To study the succession of epilithic diatoms on the artificial substrate, we investigated environmental factors and the diatom assemblages biweekly from Mar. 2004 to Feb. 2005 at 2 stations in the lower part of the Han River. A total of 60 taxa, representing 2 orders, 3 suborders, 8 families, 17 genera, 51 species, 7 varieties and 2 forms were identified, and mean number of species were 19 species in spring, 20 in summer, 22 in autumn and 22 in winter. Standing crops of epilithic diatoms varied extensively by months and stations; mean values of those were $3.2{\times}10^4$ cells $cm^{-2}$ in spring, $1.9{\times}10^4$ in summer, $1.7{\times}10^4$ in autumn and $1.8{\times}10^5$ in winter. Chlorophyll a concentrations were also similarly showed as variations of the diatom assemblages. Succession of the diatoms in St. 1 was as follows; Melosira varians, Fragilaria capucina, Cyclotella comta, Nitzschia palea in spring, Fragilaria capucina in summer, Aulacoseira granulata var. angustissima in autumn, Aulacoseira granulata var. angustissima and Melosira varians, Cymbella minuta in winter. In station 2, Aulacoseira granulata and Nitzschia palea dominated in spring as a pioneer in early stage of succession, Fragilaria capucina in summer, and Nitzschia palea in winter. According to Canonical Correspondence Analysis (CCA), there showed similar to that of succession of epilithic diatoms within St. 1 and St. 2, and they were not changed by stations but seasons. Nitzschia palea belonged to saprophilous taxa correlated with nitrogen sources and suspended solids. Meanwhile, Fragilaria capucina and Cymbella minuta included in xenosaprobic taxa show correlation with DO and pH. Eurysaprobic taxa correlated with all environmental factors.

Bacteriorhodopsin/Flavin Complex LB Films-Based Artificial Photoreceptor for Color Recognition (Bacteriorhodopsin과 flavin 복합 LB막을 이용한 색채인식기능의 인공감광소자)

  • Choi, Hyun-Goo;Jung, Woo-Chul;Min, Jun-Hong;Lee, Won-Hong;Choi, Jeong-Woo
    • KSBB Journal
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    • v.14 no.6
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    • pp.643-650
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    • 1999
  • An artificial photoreceptor composed of bacteriorhodopsin(bR)/flavin complex Langmuir-Blodgett(LB) films was developed by mimicking the human visual system. bR and flavin molecules were deposited onto solid substrate by LB technique, and the deposition of two molecules was proved by UV/VIS absorption spectroscopy and atomic force microscopy(AFM). Based on AFM images and photocurrent generation from the LB films, the optimal conditions for device fabrication were determined. With a series of light illuminations, the generated photocurrent could be detected, and the response characteristics of two molecules could be clearly distinguished from each other. According to the obtained signal shapes, three distinctive regions could be found in the obtained action spectrum. Using a correlation between the photocurrent generation and the wavelength of the input light, it was possible to organize the basic rules to interpret the wavelength of the input light. It is concluded that the proposed artificial photoreceptor would e applicable to the bioelectronic device for color recognition.

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Design of a Ultra Miniaturized Voltage Tuned Oscillator Using LTCC Artificial Dielectric Reson (LTCC 의사 유전체 공진기를 이용한 초소형 전압제어발진기 설계)

  • Heo, Yun-Seong;Oh, Hyun-Seok;Jeong, Hae-Chang;Yeom, Kyung-Whan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.5
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    • pp.613-623
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    • 2012
  • In this paper, we present an ultra miniaturized voltage tuned oscillator, with HMIC-type amplifier and phase shifter, using LTCC artificial dielectric resonator. ADR which consists of periodic conductor patterns and stacked layers has a smaller size than a dielectric resonator. The design specification of ADR is obtained from the design goal of oscillator. The structure of the ADR with a stacked circular disk type is chosen. The resonance characteristic, physical dimension and stack number are analyzed. For miniaturization of ADRO, the ADR is internally implemented at the upper part of the LTCC substrate and the other circuits, which are amplifier and phase shifter are integrated at the bottom side respectively. The fabricated ADRO has ultra small size of $13{\times}13{\times}3mm^3$ and is a SMT type. The designed ADRO satisfies the open-loop oscillation condition at the design frequency. As a results, the oscillation frequency range is 2.025~2.108 GHz at a tuning voltage of 0~5 V. The phase noise is $-109{\pm}4$ dBc/Hz at 100 kHz offset frequency and the power is $6.8{\pm}0.2$ dBm. The power frequency tuning normalized figure of merit is -30.88 dB.

Artificial Cultivation and Antioxidant Activity of Korean Native Mushroom Pycnoporus coccineus (한국산 간버섯의 인공재배 및 항산화 활성 평가)

  • Lee, Y.K.;Kim, M.K.;Yun, B.S.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.2
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    • pp.35-47
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    • 2019
  • The mycelial growth of P. coccineus strain was good in PDA and YMA, but mycelial growth was low in MEA. Light irradiation during the incubation period affected the pigment formation and density of mycelia. Mushroom of P. coccineus strain was able to produce fruiting bodies in both bottle and bag cultivation, and oak sawdust was found to be the most suitable substrate for spawn culture and cultivation. In artificial cultivation using sawdust medium, fruiting body was grown to the extent that visual observation was possible from the 15th day, and it formed about 5 days fast in the treatment group with low relative humidity. From 40 to 45 days of mushroom development, mature fruiting bodies could be harvested, and the lower relative humidity of the growing room favored mushroom development and growth. Antioxidant activity of fruiting bodies harvested from artificial cultivation showed that ABTS radical scavenging activity of bottle-cultivated and wild fruit bodies were shown at 505㎍/㎖ and 515㎍/㎖, respectively. However, fruiting bodies harvested in bag cultivation were high at 910㎍/㎖. As a result of DPPH radical scavenging activity, all extracts were found to be inactive, exhibiting IC50 value of more than 2,000㎍/㎖ concentration. The ethyl acetate extract of mushrooms obtained from bottle cultivation showed the highest activity with 1,550㎍/㎖ IC50 value. Methanol extract of wild fruit bodies had the highest ABTS radical scavenging activity at the same concentration (10mg/㎖).

Transpiration Prediction of Sweet Peppers Hydroponically-grown in Soilless Culture via Artificial Neural Network Using Environmental Factors in Greenhouse (온실의 환경요인을 이용한 인공신경망 기반 수경 재배 파프리카의 증산량 추정)

  • Nam, Du Sung;Lee, Joon Woo;Moon, Tae Won;Son, Jung Eek
    • Journal of Bio-Environment Control
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    • v.26 no.4
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    • pp.411-417
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    • 2017
  • Environmental and growth factors such as light intensity, vapor pressure deficit, and leaf area index are important variables that can change the transpiration rate of plants. The objective of this study was to compare the transpiration rates estimated by modified Penman-Monteith model and artificial neural network. The transpiration rate of paprika (Capsicum annuum L. cv. Fiesta) was obtained by using the change in substrate weight measured by load cells. Radiation, temperature, relative humidity, and substrate weight were collected every min for 2 months. Since the transpiration rate cannot be accurately estimated with linear equations, a modified Penman-Monteith equation using compensated radiation (Shin et al., 2014) was used. On the other hand, ANN was applied to estimating the transpiration rate. For this purpose, an ANN composed of an input layer using radiation, temperature, relative humidity, leaf area index, and time as input factors and five hidden layers was constructed. The number of perceptons in each hidden layer was 512, which showed the highest accuracy. As a result of validation, $R^2$ values of the modified model and ANN were 0.82 and 0.94, respectively. Therefore, it is concluded that the ANN can estimate the transpiration rate more accurately than the modified model and can be applied to the efficient irrigation strategy in soilless cultures.

Design and SAR Analysis of Wearable Antenna on Various Parts of Human Body, Using Conventional and Artificial Ground Planes

  • Ali, Usman;Ullah, Sadiq;Khan, Jalal;Shafi, Muhammad;Kamal, Babar;Basir, Abdul;Flint, James A;Seager, Rob D.
    • Journal of Electrical Engineering and Technology
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    • v.12 no.1
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    • pp.317-328
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    • 2017
  • This paper presents design and specific absorption rate analysis of a 2.4 GHz wearable patch antenna on a conventional and electromagnetic bandgap (EBG) ground planes, under normal and bent conditions. Wearable materials are used in the design of the antenna and EBG surfaces. A woven fabric (Zelt) is used as a conductive material and a 3 mm thicker Wash Cotton is used as a substrate. The dielectric constant and tangent loss of the substrate are 1.51 and 0.02 respectively. The volume of the proposed antenna is $113{\times}96.4{\times}3mm^3$. The metamaterial surface is used as a high impedance surface which shields the body from the hazards of electromagnetic radiations to reduce the Specific Absorption Rate (SAR). For on-body analysis a three layer model (containing skin, fats and muscles) of human arm is used. Antenna employing the EBG ground plane gives safe value of SAR (i.e. 1.77W/kg<2W/kg), when worn on human arm. This value is obtained using the safe limit of 2 W/kg, averaged over 10g of tissue, specified by the International Commission of Non Ionization Radiation Protection (ICNIRP). The SAR is reduced by 83.82 % as compare to the conventional antenna (8.16 W/kg>2W/kg). The efficiency of the EBG based antenna is improved from 52 to 74 %, relative to the conventional counterpart. The proposed antenna can be used in wearable electronics and smart clothing.

Development of Green Retaining-wall System with Native Evergreen Plants Corresponding to the Southern Region - A Case Study of Tongyeong City in Gyeongsangnam-do - (남부지역의 특성을 고려한 상록벽면녹화 공법 개발 -경남 통영시를 사례로-)

  • Kang Ho-Chul;Kim Kwang-Ho;Huh Keun-Young
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.2 s.109
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    • pp.32-47
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    • 2005
  • This study was carried out to investigate and propose a green-wall system with evergreen plants for urban greening of Tongyeong City. To achieve these goals, the requirements and possibilities for wall greening were investigated and evaluated considering the location, topography, and climate of Tongyeong City. Existing walls were analyzed and then a suitable green wall system is proposed. Tongyeong City and its 151 islands covers the central and the southern parts of the Goseong peninsula. Most of the land is covered with hills and mountains; $43.9\%$ of the land area has a slope greater than $15\%$ and most hills and mountains near the urban area have a slope of more than $30\%$. As a result of the topographical properties, concrete retaining walls can often be seen along the streets in urbanized areas. These retaining walls are not only unattractive, but they also create environmental problems, and thus should be replaced with native evergreen plants. Options for replacing the retaining walls include evergreen vine-plants such as Hedaa spp. and Euonymus radicans, but native evergreen shrubs such as Pittosporum tobira, Nandina domestica, Raphiolepis umbellata, Ilex cornuta, flex crenata, Fatsia japonic, and Aucuba japonica may be a more attractive option. Current wall conditions are unsuitable for planting vines, therefore, a reservoir-drainage-type plant box filled with a light artificial substrate is required for greening these concrete retaining walls. These might be irrigated in the dry season and fertilized annually by an appropriate system. These plant boxes could be attached along the entire walls. An experiment investigating effects of substrates and bark-chip mulching on the growth of Hedera spp. showed that the mixture of cerasoil and field soil(v/v, 4:6) was superior to field soil alone and to the mixture of perlite small grain, large grain, and field soil(v/v/v, 2:2:6). Bark-chip mulching tended to increase the growth of Hedera spp..

Effects of Grass Lipid and Its Fatty Acids on Ruminal Fermentation and Microbial Growth In Vitro

  • Yang, U.M.;Fujita, H.;Chung, T.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.2
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    • pp.176-181
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    • 2000
  • In order to clarify the inhibitory effects of orchardgrass (Dactylis glomerata L.) lipids on ruminal fermentation and digestion, two experiments were carried out in vitro. Experiment 1 was carried out using residues of grass hay from which the lipid fraction was removed by ether extraction. To ground grass samples were added 0, 1.5, 3.0, 4.5 and 6.0% lipids and incubated anaerobically at $39^{\circ}C$ for 24 h, with the mixtures of artificial saliva and rumen fluid. Increasing grass lipid levels remarkably reduced DM and NDF disappearances. Volatile fatty acid concentration was significantly reduced at 3.0, 4.5 and 6.0% lipid levels. Microbial nitrogen proportion to total nitrogen tended to decrease by the addition of the lipids. These results indicated that grass lipids have a marked inhibitory effect on ruminal fermentation and digestion, especially when to the substrate was added 3% or more grass lipids as ether extracts. Experiment 2 was conducted to study the relationship between changes in the free fatty acids and changes in the fermentation traits. Samples were incubated for 3, 6, 9, 12, 15, 18, 21 and 24 h as a sole substrate. The polyunsaturated fatty acids steadily decreased during incubation, whereas the saturated fatty acid ($C_{18:0}$) increased. It was suggested that the hydrogenation was extended during the initial stage of incubation. The unsaturated fatty acids ($C_{18:2}$, $C_{18:3}$) produced at the initial stage of incubation were negatively correlated with the amount of microbial N and DM disappearance, indicating that polyunsaturated fatty acids had the possibility to show an inhibiting effect on ruminal fermentation and digestion.