• The Korean Journal of Food And Nutrition
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• v.10 no.1
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• pp.117-121
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• 1997

The glucose isomerase productivity of Arthrobacter sp. L-3 was studied. glucose plus xylose showed higher productivity of glucose isomerase than any other carbon sources. Yeast extract showed higher productivity of glucose isomerase than any other nitrogen sources. The optimum culture time for the production of glucose isomerase was 40hrs.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.1
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• pp.29-33
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• 1997

The efficient production of glucose isomerase (G. I0.) produced form Arthrobacter sp. L-3 was studied. The optimum culture time of the enzyme was 40hr. The maximum enzyme activity was found at glucose concentration 1%. G. I. activity did not affect inoculum size. The glucose isomerase activity was strongly influenced by the addition of glucose.

• The Korean Journal of Food And Nutrition
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• v.4 no.2
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• pp.155-160
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• 1991

Authrobacter sp. L-3이 생성하는 glucose isomerase를 DEAE-cellulose column chromatography법으로 2단계 NaCl농도 구배로 용출함으로서 순수분리하였다. 이것이 SDS-acrylamide gel electrophoresis상에서 단일띠를 보임으로서 매우 잘 분리되었음을 알 수 있었다. Glucose isomerase의 Km값과 Vmax값이 각각 0.175M, 0.29로 얻어졌다. 한편, SDS-acrylamide gel electrophoresis와 Sephadex-G10050에 의한 gel filtration으로부터 분자량이 각각 42,000과 180,000으로 얻어져, 이 효소는 분자량이 42,500인 4개의 subunit로 구성되었음을 알 수 있었다.