• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.247-252
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• 2022

황해쑥을 MeOH : H₂O =70:30 용매로 추출하고, 얻어진 추출물을 EtOAc n-BuOH 및 water로 용매 분획 하였다. 이 중 n-BuOH 분획으로부터 silica gel (SiO₂)과 octadecyl silica gel (ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. Nuclear magnetic resonance 및 mass spectrometry 등의 스펙트럼 데이터를 통해 화합물의 화학구조를 (2S)-naringenin (1), 3-methylkaempferol (2), 3,3'-dimethylquercetin (3), and 3,3',4'-trimethylquercetin (4) 로 구조 동정하였다. 분리한 화합물 4종은 황해쑥에서는 처음 분리동정 되었다. 본 연구를 통해 glutathione mean의 증가와 glutathione heterogeneity 감소를 보인 황해쑥 유래 화합물 1-4가 세포내 글루타티온 (GSH) 수준을 균일하게 상승시키는 효능이 있음을 확인함으로써 항산화능을 확인하였다. 이러한 결과를 바탕으로 황해쑥의 기능성 소재로 활용가능성을 확인하였다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.492-502
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• 2023

This study investigated the antioxidant and immune enhancement activities of Artemisia argyi H. fermented by Lactobacillus plantarum. The fermented A. argyi H. ethanol extract increased scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), hydroxyl (·OH), and superoxide (O₂^-) radicals. Particularly, the ethanol extract of fermented A. argyi H. exhibited higher ·OH and O₂^- radical scavenging activities, compared with DPPH and ABTS⁺ radical scavenging activities. To evaluate the immune enhancement effects of the fermented A. argyi H., mice were fed a normal diet supplemented the fermented A. argyi H. at concentrations of 1%, 2%, and 5%, respectively. The supplementation of fermented A. argyi H. dose-dependently increased splenocyte proliferation. In addition, mice fed with 5% fermented A. argyi H. showed enhanced proliferation of T-cells and B-cells, along with increased levels of interferon-γ, interleukin-10, and tumor necrosis factor-α, compared to the normal group. Furthermore, mice fed with fermented A. argyi H. exhibited an increase in prominent probiotics such as Akkermansia muciniphila and Lactobacillus in gut microbiota, compared to the normal group. This study suggests that fermented A. argyi H. with Lactobacillus plantarum could be used as a dietary antioxidant and immune enhancement agent.