• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.545-555
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• 2011

The diversity elucidation by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of Gram-positive over Gram-negative bacteria within the Kangra valley in Himachal Pradesh, India. The Gram-positive bacteria observed belong to two taxonomic groupings; Firmicutes, including the genera Bacillus and Paenibacillus; and Actinobacteria, represented by the genus Microbacterium. The Gram-negative bacteria included ${\alpha}$-Proteobacteria genera Brevundimonas, Rhizobium, and Mesorhizobium; ${\gamma}$-Proteobacteria genera Pseudomonas and Stenotrophomonas; and ${\beta}$-Proteobacteria genera Azospira, Burkholderia, Delftia, Herbaspirillum and Ralstonia. The low level of similarity of two isolates, with the type strains Paenibacillus xinjiangensis and Mesorhizobium albiziae, suggests the possibility of raising species novum. The bacterial strains of different phylogenetic groups exhibited distinct carbon-source utilization patterns and fatty acid methyl ester profiles. The strains differed in their nitrogenase activities with relatively high activity seen in the Gramnegative strains exhibiting the highest similarity to Azospira oryzae, Delftia lacustris and Herbaspirillum huttiense.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.5303-5306
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• 2012

Aim: The present case-control study was conducted to explore the association of MTHFR gene polymorphism and relations of P16, MGMT and HMLH1 to MTHFR and folate intake. Methods: A total of 257 cases of esophageal squamous cell carcinoma confirmed by histopathological examination were collected. Genotyping of P16, MGMT and HMLH1 was accomplished by methylation-specific polymerase chain reaction (PCR) after sodium bisulfate modification of DNA and the MTHFR C677T genetic polymorphism was detected by PCR-restriction fragment-length polymorphism (PCR-RFLP). Results: The proportions of DNA hypermethylation in P16, MGMT and hMLH1 in cancer tissues were significantly higher than in paracancerous normal tissue. The proportion of hypermethylation in at least one gene was 88.5% in cancer tissue, and was also significantly higher than that in paracancerous normal tissue. Our finding showed individuals with homozygotes (TT) of MTHFR C677T had significant risk of DNA hypermethylation of MGMT in cancer tissues, with an OR (95% CI) of 3.15 (1.12-6.87). Similarly, patients with high intake of folate also showed a slight high risk of DNA methylation of MGMT, with OR (95% CI) of 2.03 (1.05-4.57). Conclusion: Our study found the P16, MGMT and hMLH1 demonstrate a high proportion of hypermethylation in esophageal squamous cell cancer cancer tissues, which might be used as biomarkers for cancer detection.

• Journal of Preventive Medicine and Public Health
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• v.33 no.1
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• pp.91-98
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• 2000

Objectives : This study is to evaluate the awareness, attitude, practice and the countermeasures against passive smoking in Korean adults. Methods : By self-administered questionnaires, we assessed the knowledge, attitude, behavior for passive smoking and the countermeasure for reduction of it's harmful effects in 289 men and 238 women. Results : The subjects that have heard about passive smoking were 96.8% in total and well known were 26.4% of current smoker, 56.6% of ex-smoker, and 14.8% of non-smoker(p=0.001). The irritative symptom from passive smoking was the most frequent in non-smokers and the most common place where exposed to passive smoking was public place. For attitude against passive smoking in 'no smoking allowed area', ex-smokers were the most active to recommend to stop smoking. And for opinion about establishment of 'no smoking allowed area', the restriction by law was the best acceptable method in smokers, exsmokers, and nonsmokers. In marking of 'no smoking allowed area', 69.9% of smokers answered no smoking, but in non-marking area only 6.3% stop smoking. When smokers were recommended to stop smoking, the more subjects stop smoking with good feeling in marking area, but the less in non-marking area. The factor associated the high awareness of passive smoking were aged(OR=1.07, 1.03-1.12), men(OR=4.34, 2.32-8.46). The persons who have known well about passive smoking had good attitude and behavior to prevent of harmful effect of passive smoking. Conclusions : This study suggested that education program would be necessary to reduce the passive smoking.

• The Journal of Engineering Geology
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• v.20 no.2
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• pp.169-181
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• 2010

This study aimed at simulating several responses to stresses caused by the ground water level variations around the K-1 oil stockpile. For this simulation, we considered the characteristic hydrogeological condition including the special occurrence of long and thick acidic dyke, which is regarded as the main geological structure dominating the ground water flow system at this study area. We activated twenty-four imaginary wells which are located in northern and southern area around central K-1 site. Each neighboring distance is altogether 300 m and whole distance between K-1 site and remote wells is 1,200 m. Through the modeling, we operated the long-term and continuous pumping tests and finally categorized five zones based on maximum pumping rates for the imaginary wells; zone I within 300 meter distance from K-1 site with a pumping rate of 50 $m^3/day$; zone II between 300 to 600 meter distance from K-1 site with a pumping rate of 75 $m^3/day$; zone III between 600 to 900 meter distance from K-1 site with 150 $m^3/day$; zone IV between 900 to 1,200 meter distance from K-1 site with 300 $m^3/day$; and zone V of acidic dyke area. At zone V, especially because of their possibility of high transmissivity for groundwater flow, it is necessary to control and restrict groundwater discharge.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.119-123
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• 2000

This paper studies the methods to support decision making for forest management Using GIS. Specially, most important is selecting restrict area to timber harvest suitable on scheduling its harvest in experimental forest of kangwon national university. Because timber harvesting causes water pollution, land sliding and soil erosion, it is necessary to restrict area to be harvest. The study is analyzed with Buffering modules and overlay modules which are an function of Arcinfo 7.1. As a result, restricted area is 52.64ha in total area. Of which Hardwood stand 13.21ha, pinus Pinus koraiensis stand 26.44ha, Larix leptolepis stand 12.38ha and Pinus rigida stand 0.61ha.

• Journal of the Korean Society of School Health
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• v.21 no.2
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• pp.47-60
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• 2008

Objectives: The purpose of this study was to compare Fast foods and Soft drinks consumption of Korean adolescents in a large city, a medium city, and a rural area. Methods: This was a descriptive comparative survey using a convenience sample of 2,261 8th and 11th grade students. The data were analyzed by X2-test and ANOVA using SPSS 10.0 statistical program. Results: For fast food consumptions, the higher in a large city than in a medium city and a rural area(p=.000). For soft drinks in a school, cafeteria or vending machines was higher in a large city and a medium city then in a rural area(p=.000). For eating snacks including cookies and popcorns was higher among students in a rural area than those in a medium city and large city(p=.008). Conclusions: 1. Overall, the health information among rural students is lower than those among other areas, as well as taking a health education course among rural students is also lower. Thus, the health inequality by regional differences should be considered. To decrease health inequality among different regions, health professionals who can systematically teach a health education course for middle and high school students and undertake students' health are needed. 2. Soft drinks sold in schools are higher in a large city and medium city than in a rural area. Therefore, there is a need of strong regulations and policies about the restriction of soft drinks sold in vending machines or school cafeterias.

• Journal of Energy Engineering
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• v.22 no.2
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• pp.83-89
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• 2013

Energy consumption is closely related to our lives. As Korea has four seasons, heating and cooling system is considered as essential facilities for the residential buildings. Accordingly, the government has continuously strengthened the design criteria to improve energy for new and existing building to save energy. The most important factor in the energy efficiency analysis for apartment is the heat transmission coefficient, and the value is significantly different as the heating energy amount is greatly different according to the window area ratio versus facade area ratio. Therefore, it is time to conduct continuous set-up for goal to enhance efficiency and restriction on window area ratio versus facade area ratio.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.10
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• pp.2231-2238
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• 2011

In this paper, the extract method of eye and eye-line from picture of a person is proposed. Most of existing papers are to extract the position of eyeball but in this paper, by extracting not only the position of eyeball but also eye-line, it can be applied to the face application program variously. The experimental data of the input picture is a full face photograph taken by smart phone, basically the picture is limited to the face of one person and back ground can be taken from every where and no restriction of race. The proposed method is to extract face candidated area by using Harr Classifier and set up the candidate area of eye position from face candidate area. To extract high value from eye candidate area using dilate operation, and proposed the method to classify eye and eyelash by local thresholding of the picture. After that, using thresholding image from eyemapC that Hsu's suggested, and separated the area with eye and without eye. Finally extract the contour of eye and detect eye-line using optimum ellipse estimation.

• Journal of Wetlands Research
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• v.18 no.3
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• pp.218-225
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• 2016

Various strategies and management plans have been established in order to conserve water quality as well as ecosystem, prevent deterioration of scenic area, and more importantly, retain drinking water securely. However, due to the introduction of numerous small-scale development projects on the waterside area outside protection area of source water and reparian area, river water quality and landscape are severely deteriorated. In this study, We analyzed the expected environmental impacts on the ecosystem, topograph, geology, landscape, water quality according to development type, and assigned different environmental points to each development type depend on environmental impacts, which is able to divide small-scale development project into three categories such as projects with rigorous review, projects with general review and projects with simple review. Finally, we suggested the appraisal basement is to avoid or minimize the expected environment impacts of the small-scale development projects in riparian areas so that make them sustainable development by reasonable restriction.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.9B
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• pp.1305-1313
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• 2010

Last few years, wireless personal area network (WPAN) has been widely researched for various healthcare applications. Due to restriction of device hardware (e.g., energy and memory), we need to design a highly-versatile MAC layer protocol for WBAN (Wireless Body Area Network). In addition, when an emergency occurs to a patient, a priority mechanism is necessitated for a urgent message to get through to the final destination. This paper presents a priority mechanism referred to as hybrid priority MAC for WBAN. Through extensive simulation, we show the proposed MAC protocol can minimize the average packet latency for urgent data. Thus, when patients have an emergency situation, our MAC allows adequate assistance time and medical treatment for patients. The simulation based on NS-2 shows that our Hybrid Priority MAC has the good performance and usability.