• ALGAE
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• v.29 no.1
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• pp.35-45
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• 2014

Palmaria palmata was integrated with Atlantic halibut Hippoglossus hippoglossus on a commercial farm for one year starting in November, with a temperature range of 0.4 to $19.1^{\circ}C$. The seaweed was grown in nine plastic mesh cages (each $1.25m^3$ volume) suspended in a concrete sump tank ($46m^3$) in each of three recirculating systems. Two tanks received effluent water from tanks stocked with halibut, and the third received ambient seawater serving as a control. Thalli were tumbled by continuous aeration, and held under a constant photoperiod of 16 : 8 (L : D). Palmaria stocking density was $2.95kg\;m^{-3}$ initially, increasing to $9.85kg\;m^{-3}$ after a year. Specific growth rate was highest from April to June (8.0 to $9.0^{\circ}C$), 1.1% $d^{-1}$ in the halibut effluent and 0.8% $d^{-1}$ in the control, but declined to zero or less than zero above $14^{\circ}C$. Total tissue nitrogen of Palmaria in effluent water was 4.2 to 4.4% DW from January to October, whereas tissue N in the control system declined to 3.0-3.6% DW from April to October. Tissue carbon was independent of seawater source at 39.9% DW. Estimated tank space required by Palmaria for 50% removal of the nitrogen excreted by 100 t of halibut during winter is about 29,000 to $38,000m^2$, ten times the area required for halibut culture. Fifty percent removal of carbon from the same system requires 7,200 to $9,800m^2$ cultivation area. Integration of P. palmata with Atlantic halibut is feasible below $10^{\circ}C$, but is impractical during summer months due to disintegration of thalli associated with reproductive maturation.

• Journal of Aquaculture
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• v.15 no.4
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• pp.267-273
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• 2002

Effect of heated water from a power plant in summer on hematological characteristics of flounder (Paralichthys olivaceus) were investigated. Hematocrit level fluctuated between 15.7 and 16.3% in control farm, but it significantly increased to 17.7% in the high-WT farm. The corresponding values for hemoglobin concentration were 9.9~18.0 $g/㎗$ for the control farm and 6.2~19.4 $g/㎗$ for the exposed farm. The plasma cortisol remained between 0.6 and 2.8 ng/$ml$ in the former but significantly decreased 317.1 to 3.3 ng/$ml$ in the later. The plasma glucose also decreased from 36.5~46.0 to 23.5~36.5 mg/$dl$. Plasma osmolality decreased from 551.5~597.0 to 391.0~466.5 mOsm/kg.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.4
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• pp.321-327
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• 2019

This study aimed to compare on sensory and nutritional characteristics between seasoned Laver Pyropia yezoensis with concentrates of octopus Octopus vulgaris cooking effluent (SL-COCE) and commercial seasoned laver (C-SL). Proximate composition of SL-COCE was significantly higher in moisture and ash, lower in lipid than those of C-SL. However, there was no differences in protein between seasoned lavers (P<0.05). The SL-COCE was stronger in sensory appearance, taste, flavor and texture than the C-SL. The SL-COCE was higher in mineral (Ca, P, K, Fe and Zn) than the C-SL. Total amino acid content of SL-COCE was slightly higher than that of C-SL. The major amino acids of SL-COCE were aspartic acid, glutamic acid and alanine. Mineral content based on the 100 g of SL-COCE was 272.3 mg in calcium, 392.2 mg in phosphorus, 1,025.8 mg in potassium, 10.6 mg in iron, 4.4 mg in zinc, which was higher than C-SL. Total content of fatty acid per the 100 g of SL-COCE was 35,098 mg, which was lower than C-SL (39,679 mg). The major fatty acids of SL-COCE were 16:0, 18:1n-9 and 18:2n-6.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.1
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• pp.116-123
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• 2023

Flow-through aquaculture systems generate large amounts of wastewater containing compounds such as solids that can settle near aquafarms and cause eutrophication. The settled solids are often reintroduced into flow-through systems, and aquatic animals can be affected by the solids and pathogens associated with these solids. For a sustainable aquaculture operation, adequate wastewater treatment is required. Hydrocyclones are one of the most promising technologies for the removal of solids in aquaculture wastewater. In this study, a model for performance prediction of hydrocyclones was investigated under three different operating conditions: water temperature, solids concentration, and water inlet velocity. The synthetic solids solution was prepared using settled solids from abalone aquaculture farms. The daily solids removal rates of the tested hydrocyclones ranged from 0.18 to 26.0 g solids-m^-3-day^-1, and removal efficiency ranged from 5.1 to 34.4%. The inlet water velocity had the greatest effect on solids removal and hydrocyclone efficiencies. The following multiregression model equation was derived from the daily solids removal rate (g solids-m^-3-day^-1) results for water temperature (T, ℃), solids concentration (SS, mg-L^-1), and tangential inlet water velocity (TIV, m-sec^-1): daily solids removal rate: f(z)=4.465+0.809TIV-0.375T+0.217SS (r²=0.976).

• Fisheries and Aquatic Sciences
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• v.13 no.2
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• pp.133-139
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• 2010

The effects of suspended solids size on culture water quality were determined in a commercial recirculating aquaculture system (RAS) for Japanese eel, Anguilla japonica. The particulate phase of the culture water was serially divided into six size fractions using 300, 200, 100, 75, 45, and 26 ${\mu}m$ pore size stainless sieves. The total, dissolved, and particulate nitrogen and phosphorus, and suspended solids for each fraction were determined. The concentration ranges in the fractions were: total nitrogen, 164-148 mg $L^{-1}$; total phosphorus, 20.4-15.5 mg $L^{-1}$; and total suspended solids, 8.1-6.1 mg $L^{-1}$. The concentration of total nitrogen and total phosphorus decreased significantly (P<0.05) with a 26 ${\mu}m$ and 200 ${\mu}m$ filter pore size, respectively. Nutrients from dissolved organic substances were much higher than from particulates. Analysis of particle size fractionation and its effects on water quality is useful to estimate removal efficiencies of a commercial effluent screening device for solid management and development of solid removal systems.

• Ocean and Polar Research
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• v.45 no.3
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• pp.103-111
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• 2023

To evaluate the impact of effluents from land-based fish farms on the coastal ocean of Wando, Korea, we analyzed inorganic nutrients, particulate organic carbon (POC), dissolved organic carbon (DOC), and colored dissolved organic matter (CDOM) in the effluent and influent of land-based fish farms during the summer (July) of 2021. The average concentrations of nutrients (Dissolved inorganic nitrogen, phosphorus, and silicate; DIN, DIP, and DSi, respectively) in the effluents of this study area were 17±3.7 μM, 1.4±0.7 μM, and 14±1.6 μM, respectively. The average concentrations of POC and DOC were 37±22 μM and 81±13 μM, respectively, with POC accounting for about 30% for total organic carbon in effluents. The Reduced Dissolved Inorganic Nitrogen/Total Dissolved Inorganic Nitrogen ratio (0.7), potential short-period index, indicates that the discharge of nutrients excreted by the fish and unconsumed feed into coastal water results in such nutrients being deposited and accumulated in the sediment. Subsequently, this continuous accumulation triggers the release of ammonium ions during organic matter decomposition, and the ammonium-enriched waters that encroach on fish farms as influent seem to be due to the diffusion of high concentrations of ammonium from bottom sediment. Furthermore, we used fluorescence indices to examine the characteristics of organic matter sources, obtaining mean values of 1.54±0.19, 1.06±0.06, and 1.56±0.06 for the humification index, biological index, and fluorescence index, respectively, in the effluent. These results indicate that the organic matters had an autochthonous origin that resulted from microbial decomposition, and such organic matters were rapidly generated and removed by biological activity, likely supplied from the sediment. Our results suggest that the effluent from land-based fish farms could be a potential source of deoxygenation occurrence in coastal areas.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.242-249
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• 1998

The engineering aspect of water treatment processes in the recirculating aquaculture system was studied. To recycle the water in the aquaculture system, a wastewater treatment process was required to maintain high water quality for the growth and health of the cultured fish. In this study, three different biofilm processes were used to reduce the concentration of organic matters and ammonia from the recirculating water - two phase fluidized bed, three phase fluidized bed, and trickling filter. The objectives of this research were to evaluate the optimum treatment conditions of the biofilm processes for the recirculating aquaculture system, and thereby reduce the volume of biofilm processes, which are commonly used for the recycle water treatment processes for aquaculture. The result of this study showed that the removal efficiency of organic matters by trickling filter was found to be lower than that of the fluidized bed. In the trickling filter system, anthracite showed better organic removal efficiency than crushed stone as a media. In the two phase fluidized bed, the maximum removal efficiency of either organics or ammonia was obtained when both the packing rate of media was maintained to $40\%$ of total reactor depth excepting sediment zone and the bed expansion rate was maintained to $100\%$. When 100 tilapia (Oreochromis niloticus) of each average 200g was reared, the pollutant production rate was 0.07g $NH_4\;^+-N/kg$ fish/day and 0.06g P04-3-P/kg fish/day, and sludge production rate was 0.39 g SS/kg fish/day. In the two phase and three phase fluidized bed, the volume of water treatment tank could be calculated from an empirical equation by using the relationship between the influent COD to $NH_4\;^+-N$ ratio (C/N, -), media concentration (Cm, g/L), influent ammonia nitrogen concentration (Ni, mg/L), effluent ammonia nitrogen concentration (Ne, mg/L), bed expansion rate $(E,\;\%)$, and influent flowrate $(Q,\;m^3/hr)$. The empirical equation from this study is $$V_2\;=\;10^{3.1279}\;C/N^{3.5461}\;C_m\;^{-3.7473}\;N_i\;^{4.6477}\;E^{0.0326}\;N_e\;^{-0..8849}\;Q\;(Two\;Phase\;FB) V_3\;=\;10^{11.7507}\;C/N^{-1.2330}\;C_m\;^{-6.5715}\;N_i\;^{1.5091}\;N_e\;^{-1.8489}\;Q (Three\;Phase\;FB)$$

• Journal of environmental and Sanitary engineering
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• v.18 no.1
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• pp.15-22
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• 2003

Treatability tests were conducted using EMC process to study the feasibility of applying this process as recycling-water treatment system in high density seawater aquaculture farm. To study the effect of organic and ammonia nitrogen loading on system performance, hydraulic retention time of reactor was reduced gradually from 12hr to 10min. The conclusions are can be summarized as follows. When the system HRT was reduced from 12hr to 10 min gradually, there was little noticeable change(reduction) in ammonia nitrogen removal efficiencies until 2hr of HRT, however, removal efficiencies were decreased dramatically when the system was operated under the HRT of less than 2hr. In case of organics(COD), there was no dramatic deterioration in removal efficiencies depending on HRT reduction. More than 90% of removal efficiencies were maintained successfully when the system was operated at the HRT of 10 min. In case of system performance depending on media packing ratio in reactor, there was little difference in each reactor performance depending on media packing ratio in reactor when the reactors were operated under the HRT of longer than 1hr, however, differences in reactor performances were considerably evident when the reactors were operated under the HRT of shorter than 1hr. That is, the more reactor was packed, the better reactor performed. When comparing reactor performance among 25%, 50%, 75% packed reactor, it can be judged that media packing ratio more than 50% plays no significant role in increasing reactor performance. For this reason, packing the media less than 50% is more reasonable way in view of economic. Such a tendency well agreed with the variation of ammonia-nitrogen removal efficiencies according to the media packing ratio in reactors at each HRT. Difference in effluent ammonia-nitrogen concentration between 50% media packing reactor and 75% media packing reactor was negligible. When comparing with the results of 25% packing reactor, difference was not so great.

• Fisheries and Aquatic Sciences
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• v.13 no.2
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• pp.150-156
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• 2010

The separation performance of a low-pressure hydrocyclone (LPH) was evaluated for suspended-solids removal in a recirculating aquaculture system (RAS). The dimensions of the LPH were 335 mm cylinder diameter, 575 mm cylinder height, 60 mm overflow diameter, 50 mm underflow diameter, and $68^{\circ}$ cone angle. The inflow rate varied (400, 600, 800, and 1,000 mL $s^{-1}$) with 25%, 25%, 20%, and 10% of bypass ($R_f$), respectively. The maximum total separation efficiency (Et) and reduced separation efficiency (E't) for suspended solids from the effluent of the second settlement tank (before biofiltration) were 58.9% and 45.2%, respectively, at an inflow rate of 600 mL $s^{-1}$ and 25% of $R_f$. The maximum Et and E't for suspended solids from the water supply channel (after biofiltration) were 24.4% and 16%, respectively, at an inflow rate of 1,000 mL $s^{-1}$ and 10% of $R_f$. The maximum grade efficiency (Ei) was 51.6% for a 300 ${\mu}m$ particle size at an inflow rate of 600 mL $s^{-1}$ with 23% of $R_f$. The maximum reduced grade efficiency (E'i) was 37.6% for a 300 ${\mu}m$ particle size at an inflow rate of 1,000 mL $s^{-1}$ with 11% of $R_f$. The results indicate that the separation performance of the LPH for suspended solids removal was size selective and that maximum removal occurred at particle sizes ranging from 300 to 500 ${\mu}m$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.428-437
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• 2023

A simultaneous analytical method was developed and validated for the analysis of prohibited fluoroquinolone (FQ) antibiotics including norfloxacin, ofloxacin, and pefloxacin, released by aquaculture in seawater and effluents. The samples were filtered, and extracts were obtained using a solid phase extraction cartridge with methanol (MeOH). The extracts were concentrated, and analyzed using ultra-performance liquid chromatography-tandem mass spectrometry. Two different columns and four different mobile phases were compared to achieve optimal separation and sensitivity for target compounds. Typical validation parameters including linearity, recovery of surrogate standard, instrument detection limit (IDL), limit of quantification (LOQ), and method detection limit (MDL) were evaluated. The linearity of calibration curves was over 0.999. Recoveries of surrogate ranged from 87.6% to 113%. The LOQ of target compounds was approximately 3-8 times lower than those reported in previous studies. The IDL and MDL were 0.06-0.57 and 0.06-0.37 ng/L, respectively. Seven effluent samples collected from an aquaculture located in Jeju were analyzed; however, not all target compounds were detected in the samples, suggesting that the banned antibiotics were not used. Overall, this established method was able to simultaneously analyze the three FQ antibiotics, and may be useful for monitoring prohibited antibiotics in the fishery industry.