• 제목/요약/키워드: Appressorium

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Evaluation of Streptomyces padanus IA70-5 Strain to Control Hot Pepper Anthracnose (Colletotrichum acutatum) (고추 탄저병 (Colletotrichum acutatum) 방제를 위한 Streptomyces padanus IA70-5의 평가)

  • Chi, Tran Thi Phuong;Choi, Okhee;Kwak, Youn-Sig;Son, Daeyoung;Lee, Jeung Joo;Kim, Jinwoo
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.37-45
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    • 2012
  • To select bacterial strains with antifungal activity against an anthracnose fungal disease causing damage severely on hot pepper, previous isolates obtained from plant root samples were screened. Among 457 isolates, IA70-5 isolate was finally selected and identified as Streptomyces padanus based on 16S rDNA sequence analysis. Strain IA70-5 is non-pigmenteous, non-mobile, and filamentous. S. padanus IA70-5 inhibited effectively the mycelium growth, spore germination, and appressorium formation of Colletotrichum acutatum in vitro. The results of this study demonstrated that IA70-5 strain, especially applied on fruit of hot pepper, decreased disease incidence 90% for pre-inoculation before pathogen treatment. Taken together, S. padanus IA70-5 strain is a promising biological control agent to control of a major fungal pepper disease, anthracnose.

An Histopathological Investigation on the Resistant Tong-Il Cultivar by Inoculating Rice Blast Fungus, Pyricularia oryzae Cav. (수도(水稻) 도열병(稻熱病)에 저항성(抵抗性)인 통일품종(統一品種)에 대한 침입생리(侵入生理)에 관(關)한 연구(硏究))

  • Chung, Bong-Koo;Kim, Kwang-Suk
    • The Korean Journal of Mycology
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    • v.5 no.1
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    • pp.13-19
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    • 1977
  • In order to investigate histopathologically the nature of varietal resistance and infection process of the rice bast fungus, Pyricularia oryzae. this experiment was undertaken by using the resistant cultivar Tongil and susceptible cultivars Norin No.6 and Jinheung in 1973 to 1974. 1) It was found that appressorium of the fungus forms not only at 4 hours after incubation under the favorable conditions but also peak of appressorial formation is at 48 hours treatment. Physical stimulus known to be a definite factor for appressorial formation. The optimum temperature range for appressorial infection was at $24^{\circ}C$ to $28^{\circ}C$, and pH was between 4.8 to 8.0 with 6.8 as the optimum. 2) Although percent of appressorial formation on the leaves of resistant Tongil and susceptiible Norin No.6 were only slightly different, there was a remarkable difference between resistant and susceptible cultivars with regard to percent of hyphal infection and index for hyphal extension. Index of hyphal extension was 1.6-2.7 in Tonyil. while in susceptible cultivar was 3.4-6.6. The rate of discoloration of infected cells, a indication of hypersensitivity, was greater in the resistant than in susceptible cultvar. 3) Therefore, it could be concluded that resistance of Tongil cultivar may be attributable to a higher degree of mechanical barriers as well as a higher level of antifungal substance accumulation.

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