• BMB Reports
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• 제30권4호
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• pp.274-279
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• 1997

Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of branchedchain amino acids, valine, leucine, and isoleucine. ALS is the target site for several structually diverse classes of herbicides including sulfonylureas, imidazolinones. and triazolopyrimidines. We have purified ALS from etiolated barley shoots to homogeneity. The five major purification steps are ammonium sulfate fractionation, DEAE anion exchange, hydroxylapatite, Bio-Gel A gel filtration, and low pressure Mono-Q chrornatoqraphy. Approximately 170-fold purification was achieved and the yield was 0.45% of initial activity in the crude extract. Both SDS-PAGE and Western blot analysis showed a single polypeptide of ALS with an apparent molecular mass of 64 kDa. The result of nondenaturing gel electrophoresis with activity staining indicated that the molecular mass of its native form is approximately 225 to 250 kDa. The values of $K_m$ for pyruvate. pl. and optimum pH of ALS were determined to be 2.0 mM, 5.2. and 7.0. respectively Feedback inhibition studies showed that ALS is more susceptible to leucine than valine. And $IC_{50}$ value of Cadre, a class of irnidazolinones, is about $1.5\mu{M}$ for ALS.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.258-263
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• 1998

${\beta}$-Xylosidase was purified from the recombinant Escherichia coli carrying the Bacillus sp. KK-1 ${\beta}$-xylosidase gene (xylB). The molecular mass of the purified enzyme was estimated to be 62 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, the apparent molecular mass of the ${\beta}$-xylosidase was 140 kDa, indicating that the native ${\beta}$-xylosidase has an oligomeric structure composed of two identical subunits. The isoelectric point was determined to be pH 5.5. The enzyme was highly active on p-nitrophenyl-$\beta$-D-xylopyranoside but it barely hydrolyzed xylan substrates, and did not exhibit activity towards carboxymethylcellulose and p-nitrophenyl-${\beta}$-D- glucopyranoside. The enzyme had a pH optimum for its activity at pH 6.5 and a temperature optimum at $40^{\circ}C$. The enzyme activity was completely inhibited by the presence of $Hg^{++}$, and also markedly inhibited by D-xylose and D-glucose.

• Journal of Microbiology
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• 제34권2호
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• pp.206-213
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• 1996

NADH-quinone reductase was purified 22-fold from the cytosolic fraction of Streptomyces sp. Imsnu-1 to apparent hemogenity, with an overall yield of 9%, by the purification procedure consisting of ammonium, sulfate precipitation and DEAE Sephacryl S-200 and DEAE 5 PW chromatographies. Thes molecular mass of the enzyme determined by gel filtration chromatography was found to be 110 kDa. SDS-PAGE revealed that the enzyme consists of two sugunits with a molecular mass of 54 kDa. The enzyme contained 1 mol of FMN per subunit as a cofactor. The $A_{272}$ A$_{457}$ ratio was 6.14 and the molar extinction coefficients were calculated to be 20, 800 and 25, 400M$^{-1}$ $cm^{-1}$ / AT 349 AND 457 nm, respectively. The N-terminal sequence of the enzyme contained the highly conserved fingerprint of ADP-binding domain. The enzyme used NADH as an electron donor and various quinones as electron acceptors. Cytochrome c was practically inactive. Air-stable flavin semiquinone was produced by the addition of NADH to the enzyme. Also, naphthosemiquinone was detected in the reaction mixture containing the enzyme.

• 자원환경지질
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• 제24권1호
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• pp.63-69
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• 1991

Gravity study was carried out to investigate the structure and total mass of the Bupyeong silver deposits closely related to formation of the Bupyeong caldera. Survey region covers $3.3{\times}6.6km^2$ over silver deposits and is comprised of 334 gravity measurement stations. An apparent regional gravity trend parallel to the west coast line is mainly attributed to isostasy. A least square isostasy model was used for the regional correction. A Fortan subroutine was coded to calculate 3-dimensional subsurface model. The calculated gravity values from the 3-dimensional model of the caldera with silver deposits agree with observed anomalies relatively well. Gravity anomaly due to Bupyeong silver deposits reaches to +3.5 mgal from the background value and anomaly due to the caldera reaches to -4 mgal. But the maximum negative anomaly of the caldera would be much greater at its center. The total mass of silver deposits calculated from the subsurface model is $4.19{\times}10^9$ tons. Although the economic part of silver deposits depends on the grade of orebody, we expect that there are still large amount of silver reserves in Bupyeong area.

• 한국전기전자재료학회논문지
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• 제15권2호
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• pp.141-146
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• 2002

We synthesized polypyrrole (PPy) by electrolysis of the pyrrole monomer solution containing support electrolyte, KCl and/or p-toluene sulfonic acid sodium salt (p-TS). The electrochemical behavior, was investigated using cyclic voltammetry and AC impedance. In the case of using electrolyte p-TS, the oxidation potential of the PPy was about -02 V vs Ag/AgCl reference electrode, while the potential was about 0 V for using electrolyte KCl. The falloff of the oxidation potential gave a sign of an improvement in the electron hopoing mechanism on the backbone. The AC impedance plot gave a hint of betterment of mass transport. PPy doped with p-TS improved in mass transport or diffusion. That was because the PPy doped with p-TS was more porous than PPy with KCl. We attained an effect of good kinetic parameters, in the case of PP-GOx enzyme electrodes doped with p-TS, which were determined by 58 mmol dm$\^$-3/ for apparent Michaelis constant and by 581 ㎂ for maximum current respectively.

• 천문학회보
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• 제38권1호
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• pp.31.2-31.2
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• 2013

We use grid-based hydrodynamic simulations to study star formation in nuclear rings in barred galaxies. The gaseous medium is assumed to be infinitesimally thin, isothermal, and unmagnetized. To investigate various situations, we vary the total gas content in the bar regions and the bar growth time. We find that star formation rate (SFR) in a nuclear ring is determined by the mass inflow rate to the ring rather than the total gas mass in the ring. The SFR shows a strong primary burst and weak secondary bursts at early time, and declines to small values at late time. The primary burst is caused by the rapid gas infall to the ring due to the bar growth, with its duration and peak depending on the bar growth time. The secondary bursts result from re-infall of the ejected gas by star formation feedback of the primary burst. When the SFR is low, ages of young star clusters exhibit an azimuthal gradient along the ring since star formation takes place mostly near the contact points between the dust lanes and the nuclear ring. When the SFR is large, on the other hand, star formation is widely distributed throughout the whole length of the ring, with no apparent age gradient of star clusters. Regardless of SFR, star clusters have a positive radial age gradient, with younger clusters located closer to the ring, since the ring shrinks in size over time.

• 여성건강간호학회지
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• 제25권2호
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• pp.129-142
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• 2019

Purpose: The purpose of this study was to investigate the relationship between obesity, body image perception, and weight control for obesity management in adult women. Methods: The subjects of this study were 3,617 women aged over 19 years, who participated in the Seventh Korea National Health and Nutrition Examination Survey 2016. Data were analyzed through complex sampling design data analysis. Results: Right body image perception according to obesity was apparent between 70.6-81.7% of women and 76.5% of women with abdominal obesity perceived that they were normal. Obese women performed more weight control than non-obese women. Women with only abdominal obesity performed less weight control than non-obese women, and 47.3% of them performed weight control. Women who perceived themselves as obese performed more weight control than women who perceived themselves as non-obese (odds ratio, 2.08; confidence interval, 1.69-2.57), but body mass index was not observed to be associated with weight control. Conclusions: Education on abdominal obesity should be provided to increase awareness about abdominal obesity and its effective management, especially in women with only abdominal obesity. In addition, interventions for right body type perception should be provided for proper weight control along with prevention and management of obesity.

• Korean Journal of Radiology
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• 제24권1호
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• pp.62-78
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• 2023

As the majority of incidentally detected lesions in the anterior mediastinum is small nodules with soft tissue appearance, the differential diagnosis has typically included thymic neoplasm and prevascular lymph node, with benign cyst. Overestimation or misinterpretation of these lesions can lead to unnecessary surgery for ultimately benign conditions. Diagnosing mediastinal cysts using MRI serves as a problem-solving modality in distinguishing between surgical and nonsurgical anterior mediastinal lesions. The pitfalls of MRI evaluation for anterior mediastinal cystic lesions are as follows: first, we acknowledge the limitation of T2-weighted images for evaluating benign cystic lesions. Due to variable contents within benign cystic lesions, such as hemorrhage, T2 signal intensity may be variable. Second, owing to extensive necrosis and cystic changes, the T2 shine-through effect may be seen on diffusion-weighted images (DWI), and small solid portions might be missed on enhanced images. Therefore, both enhancement and DWI with apparent diffusion coefficient values should be considered. An algorithm will be suggested for the diagnostic evaluation of anterior mediastinal cystic lesions, and finally, a management strategy based on MRI features will be suggested.

• 한국수산과학회지
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• 제46권4호
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• pp.407-412
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• 2013

We performed four sets of feeding trials to establish optimum feed size (Exp-I), stocking density (Exp-II) and feeding frequency (Exp-III and IV) for Korean rockfish. In Exp-I, three different particle sizes of a commercial diet (small, 2.0-2.1 mm; medium, 2.4-3.2 mm; and large, 4.0-5.3 mm) were fed to four replicate groups of fish ($22.8{\pm}0.1g$), each of which was fed to apparent satiation for six weeks. In Exp-II, fish ($44.3{\pm}0.4g$) were reared at four stocking densities (1.5, 3.0, 4.5 and 6.0 $kg/m^3$) and fed a commercial diet to apparent satiation for four weeks. In Exp-III, fish ($14.8{\pm}0.1g$) were fed a commercial diet once, twice, thrice, $4{\times}$ or $5{\times}$ a day at a feeding ratio of 3.25% of body mass. Another group of fish was fed the same commercial diet $5{\times}$ a day to apparent satiation; this treatment was designated FS (five times satiation/day). In Exp-IV, fish ($31.3{\pm}0.1g$) were fed a commercial diet once, twice, thrice or $4{\times}$ a day to apparent satiation. Another group of fish was fed to apparent satiation once every 48 hours. In Exp-I, fish fed the large particle (4.0-5.3 mm) diet had a significantly higher feed conversion ratio and lower protein efficiency ratio than fish fed the small particle diet. In Exp-II, groups of fish reared at densities of 4.5 and 6.0 $kg/m^3$ had significantly higher feed intake and growth performance than fish reared at 1.5 and 3.0 $kg/m^3$. In Exp-III, fish fed to apparent satiation had significantly higher growth performances than fish fed once or $4{\times}$ a day. A significantly higher feed conversion ratio and a lower protein efficiency ratio were obtained in the FS group. In Exp-IV, growth performance and feed utilization efficiency were not significantly affected by experimental variation in feeding frequency. Fish fed to apparent satiation once every 48 hours had better feed utilization than those in other treatments and growth performances of those were comparable. Therefore, the optimum feed particle size, stocking density and feeding frequency for Korean rockfish under conditions we used were 2.0-3.2 mm, 4.5-6.0 $kg/m^3$, and once a day or once every 48 hour, respectively.

• Journal of Mechanical Science and Technology
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• 제15권4호
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• pp.492-499
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• 2001

The characteristics of Prand시-Meyer expansion of supersonic flow with condensation along a wavy wall in a channel are investigated by means of experiments and numerical analyses. Experiments are carried out for the case of moist air flow in an intermittent indraft supersonic wind tunnel. The flow fields are visualized by a Schlieren system and the distributions of static pressure along the upper wavy wall are measured by a scanning valve system with pressure transducers. In numerical analyses, the distributions of streamlines, Mach lines, iso-pressure lines, and iso-mass fractions of liquid are obtained by the two-dimensional direct marching method of characteristics. The effects of stagnation temperature, absolute humidity, and attack angle of the upper wavy wall on the generation and the locations of generation and reflection of an oblique shock wave are clarified. Futhermore, it is confirmed that the wavy wall plays an important role in the generation of an oblique shock wave and that the effect of condensation on the flow fields is apparent.