• Asian-Australasian Journal of Animal Sciences
• /
• 제8권5호
• /
• pp.433-441
• /
• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권6호
• /
• pp.671-678
• /
• 1997

The objective was to evaluate method of feeding (free access or intubation), method of slaughter (carbon dioxide gas or barbiturate) and digesta flushing medium (distilled water or physiological saline), in the development of an ileal amino acid digestibility assay for 4 week-old broiler chickens. Three diets were used (commercial (C), semi-synthetic meat-and bone meal (MBM) or wheat (W)). For the coarser C and W diets but not for the MBM diet, feeding method had a significant effect on concentrations of chromium (Cr), nitrogen (N), acid detergent fibre (ADF) and neutral detergent fibre (NDF) in the crop contents at a set time after a meal. There appeared to be a selection of food particles under free-access feeding. For birds receiving the wheat diet there was an effect (p < 0.05) of sampling time after feeding on the concentrations of Cr, N, ADF and NDF/Cr in the crop contents. Flushing ileal digesta with distilled water or saline led to similar apparent ileal N digestibility coefficients. Birds given the MBM diet, and killed by inhalation of $CO_2$, had significantly (p < 0.05) lower apparent ileal N digestibility coefficients (73 versus 80%) than those killed by barbiturate overdose.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권11호
• /
• pp.1496-1502
• /
• 2010

Two experiments were conducted to evaluate the effects of diet complexity and fermented soy protein on growth performance and amino acid digestibility. In Exp. 1, a total of 120 crossbred weanling pigs ($5.68{\pm}0.80\;kg$ BW) were randomly allocated into 4 treatments. Each treatment had 6 replicate pens comprising 5 pigs in each replicate. Experimental diets consisted of simple (soybean meal as protein source) and complex (soybean meal, rice protein concentrate, potato protein concentrate and fish meal as protein sources) diets; each diet contained 0 or 5% fermented soy protein (FSP), respectively. Dietary treatments included: i) simple diet; ii) simple diet with 5% FSP; iii) complex diet; iv) complex diet with 5% FSP. Pigs were provided each experimental diet for 20 d (phase 1) and then fed the same common diet for 10 d (phase 2). During days 0-10, pigs fed FSP diets had greater ADG than those fed non-FSP diets (p<0.05). G/F in FSP treatments was significantly higher than that in non-FSP treatments (p<0.05) from days 0 to 10. Throughout the overall period, G/F was greater in FSP treatments compared with non-FSP treatments (p<0.05). On d 10, N digestibility was higher in pigs fed FSP diets than in those fed non-FSP diets (p<0.05). Diet complexity did not affect growth performance and nutrient digestibility (p>0.05) in this experiment. In Exp 2, 12 ileal-cannulated, weanling barrows were housed in individual metabolism crates and randomly assigned to 1 of 4 treatments (same as Exp. 1) by using a $4{\times}4$ Latin square design. Among the essential amino acids, apparent ileal digestibility (AID) of Met and Val were increased in pigs fed FSP diets compared with those fed non-FSP diets (p<0.05). AID of Met, Phe and total essential amino acids were higher in pigs fed complex diets than in those fed simple diets (p<0.05). Among the non-essential amino acids, AID of Ala in FSP treatments was greater than that in non-FSP treatments (p<0.05). In addition, Asp, Cys, Glu, Pro, Ser and total non-essential amino acid digestibilities in pigs fed complex diets were higher compared with those fed simple diets (p<0.05). Interaction was observed in AID of Met, Asp and Pro. In conclusion, these results indicated that feeding of 5% FSP to nursery pigs improved feed efficiency and AID of amino acids, and diet complexity did not maximize the growth performance of pigs in the subsequent phase.

• Journal of Animal Science and Technology
• /
• 제51권1호
• /
• pp.15-24
• /
• 2009

본 연구는 다양한 유기산제들의 급여가 이유자돈의 성장과 영양소 소화율에 미치는 영향을 비교하기 위하여 2번의 사양실험을 수행하였다. 실험 1과 실험 2에서는 각각 이유자돈 180두 (4처리, 3반복, 15두/반복)를 공시하였다. 실험 1은 기초사료에 formic acid, ammonium-formate, lactic acid 그리고 acid mixture를 각각 0.5%씩 첨가하여 총 5주간 사양실험을 실시하였으며, 실험 2는 formic acid, ammonium-formate, lactic acid 그리고 acid mixture를 각각 0.3%씩 첨가하여 총 6주간 사양실험을 실시하였다. Acid mixture는 formic acid와 lactic acid가 50:50의 비율로 혼합된 제제를 사용하였다. 외관상 회장아미노산 소화율을 측정하기 위하여 각 처리별 3두, 총 12두를 공시하여 T-cannula를 회장 말단부에 부착시킨 후 적응기간을 거쳐 회장내용물을 채취하였다. 사양성적은 유기산 종류에 따른 개선 효과는 없는 것으로 나타났으나 (실험 1), acid mixture 급여구에서 증체량이 lactic acid 급여구보다 높은 것으로 나타났다 (실험 2). 외관상 회장 아미노산 소화율은 lactic acid 급여구보다 acid mixture 급여구에서 개선되는 것으로 나타났다 (실험 1과 2). 실험 1과 2의 결과를 종합하였을 때 acid mixture (formic acid + lactic acid) 급여는 이유자돈의 사양성적과 외관상 회장 아미노산 소화율을 개선시키는 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제10권6호
• /
• pp.635-642
• /
• 1997

This experiment was conducted to determine apparent ileal and fecal digestibilities of some grains for young pigs (15.6 kg BW). Ileal and fecal digestibility of corn, milo, wheat, barley, rice and tapioca were measured with pigs fitted with simple ileal T-cannula. Rice was the highest (p < 0.05) and tapioca was the lowest (p < 0.05) in the digestibilities of gross energy. For the crude protein digestibilities, rice, barley and wheat were higher (p < 0.05) than corn, milo and tapioca. The average ileal digestibilities of essential amino acids (AAs) were 89.9, 85.8, 81.5, 80.9, 80.2 and 76.3% for those fed rice, barley, milo, wheat, corn and tapioca diets, respectively. Regardless of the dietary carbohydrate sources, among the dispensable amino acids, glycine had lowest digestibility except for barley and milo. The apparent ileal digestibility of lysine and methionine were 90.7, 94.0% in rice, 88.3, 86.5% in barley and 86.3, 82.3% in wheat. The apparent fecal digestibility of average essential AAs were 90.8, 89.9, 87.7, 85.1, 82.5 and 77.9% in pigs fed the rice, barley, wheat, corn, milo and tapioca diets, respectively. The fecal digestibilities of essential AAs and nonessential AAs, in general, were higher than the ileal digestibilities, which indicating a loss of nitrogenous components in the cecum and colon. For all AAs, differences between ileal and fecal amino acid digestibilities ranged from 0.0 (valine) to 5.8 (threonine) in rice, 0.1 (arginine) to 14.5 (glycine) in barley, 2.8 (lysine) to 12.4 (glycine) in wheat, 1.0 (isoleucine) to 12.9 (glycine) in corn, 0.2 (serine, glutamic acid) to 6.5 (methionine) in milo and 0.5 (valine) to 22.1 (glycine) percentage units in tapioca diets. In conclusion, whether it is ileal or fecal, rice and barley appeared to be excellent alternative carbohydrate sources for young pigs in terms of AAs digestibility. However, energy value and cost for each grain should be considered when formulating diets.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권10호
• /
• pp.1442-1449
• /
• 2017

Objective: This study investigated the effect of microbial phytase supplementation on growth performance, tibia ash, plasma parameters, apparent ileal digestibility (AID) of amino acid (AA) and apparent digestibility of nutrients in Youxian Sheldrakes fed with low-phosphorus (P) cornsoybean diets. Methods: A total of 350 Youxian Sheldrakes (7d old) were randomly divided into 5 treatment groups: positive control (PC) group has adequate available P diet (0.42% and 0.38%, starter and grower), negative control (NC) group were deficient in available P (0.32% and 0.28%, starter and grower) and NC diet was supplemented with 3 levels of microbial phytase (500, 750, and 1,000 U/kg). Results: Dietary supplementation of phytase in NC diet improved the average daily gain, increased the levels of serum calcium (Ca), tibia Ca and P, AID of AA and apparent digestibility of energy and Ca in starter stage (p<0.05). There was an increased (p<0.001) in the utilization of P from 17.3% to 23.9%. Phytase supplementation (1,000 U/kg) has shown that the AID of His, Thr, Val, indispensable AA, Glu, Pro, and dispensable AA was higher (p<0.05) than that of NC. Moreover, phytase supplementation improved (p<0.05) serum and tibia Ca and P, AID of AA and apparent digestibility of dry matter, crude protein, energy, P and Ca, and reduced (p<0.05) feed to gain ratio (F/G) and the levels of serum alkaline phosphatase in grower stage. Likewise, an increase (p<0.001) in the utilization of P was noticed from 12.6% to 17.2%. Supplement phytase at 750 U/kg improved the AID of His, Thr, Asp, Cys, Pro, and Ser (p<0.05). Conclusion: The microbial phytase supplement could improve growth performance, AID of some AA and apparent utilization of other nutrients in Youxian Sheldrakes, and reduce excreta P load to environment.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권8호
• /
• pp.1167-1173
• /
• 2009

This study was conducted to evaluate the effects of providing fermented soy protein to weaned pigs on nitrogen balance and apparent fecal and apparent ileal digestibility (AID) of AA. Four weaned ((Yorkshire${\times}$Landrace)${\times}$Duroc) barrows (BW = 6.58${\pm}$0.98 kg), surgically fitted with a simple T-cannula approximately 15 cm prior to the ileo-cecal junction, were fed four diets according to 4${\times}$4 Latin square design. Diets were a basal diet supplemented with one of the following: 3% SDPP (spray dried plasma protein), 5% RBP (soy protein fermented by Lactobacillus spp.), 5% PSP (soy protein fermented by Aspergillus oryzae and Bacillus subtilis), and 2.5% RPP (2.5% RBP+2.5% PSP). No differences were observed in DM and N intakes among treatments. However, the level of urine excretion was greater in the RPP group than in the PSP group. Additionally, fecal DM excretion, fecal N concentration and fecal N excretion were increased in the RBP, PSP and RPP groups when compared with the SDPP group (p<0.05). Furthermore, total excretion was increased in the RPP group when compared with the PSP group (p<0.05). In addition, N absorption and the N absorption ratio were higher in the SDPP group than in the RPP group (p<0.05). Moreover, the DM and N digestibilities were lower in the RBP, PSP and RPP groups than in the SDPP group (p<0.05), and the ash and energy digestibilities were higher in the SDPP and RBP groups than in the PSP and RPP groups (p<0.05). However, no significant differences were observed in the DM, N, Ash, Ca, P or ileal digestibilities among treatments, although the energy digestibility was higher in the SDPP group than the RBP group (p<0.05). In addition, the apparent ileal digestibilities of essential amino acids (Arg, His, Iso, Leu, Lys, Phe, Thr, and Val) were significantly higher in the SDPP group than in the other groups (p<0.05), and the levels of Ala, Cys, Glu and Try were greater in the SDPP treatment group than the RBP, PSP and RPP groups (p<0.05). Additionally, the levels of Asp, Gly and Ser were higher in the SDPP group than the PSP and RPP groups, and the level of Pro was higher in the SDPP group than the RPP group (p<0.05). Finally, total non-essential amino acid and total amino acid digestibility were higher in the SDPP group than in the other treatments (p<0.05). Taken together, the results of this study indicate that animal protein is more bioavailable than plant protein. However, the N absorption ratio and ileal digestibility were found to be similar in the SDPP and RBP groups.

• Preventive Nutrition and Food Science
• /
• 제3권2호
• /
• pp.180-185
• /
• 1998

A regression equation was proposed for predicting protein digestibility using pH drop and free amino acid content. Results were compared with those determined by the pH drop method of Satterle et al. and with apparent in vivo digestibility in rats. Measurd free amino acid content prior to four enzyme digestion had an influence on calculating digestbiilty . Results from new equation correlated more highly (r=0.8434, difference average=2.304) with in vivo digestibility than the results of pH drop method (r=0.7603, difference average=10.099).

• Journal of Animal Science and Technology
• /
• 제64권4호
• /
• pp.740-751
• /
• 2022

Two experiments were conducted to determine the effects of bird age on apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) for 10-d-old Experiment (Exp. 1) and 22-d-old (Exp. 2) male broilers. This study investigated the effects of different broiler ages and feed ingredients on AID and SID of AA in corn and soybean meal (SBM). Four hundred and eighty (age = 7 d; initial body weight [BW] = 173.4 ± 12.65 g) and 192 (age = 18 d; initial BW = 772.2 ± 62.13 g) birds were allocated to three dietary treatments in a randomized complete block design with eight replicate cages per treatment. Two diets were formulated based on corn or SBM as the sole source of AA in the diet. A nitrogen-free diet was also formulated to measure basal endogenous losses of AA. Experimental diets were given for 3 and 4 days in Exps. 1 and 2, respectively. An interaction was observed (p < 0.05) between the age of birds and the type of ingredient for the AID of most AA, except for methionine, valine, cysteine (Cys), and tyrosine; however, the effects of age and type of ingredients were diminished in the SID of AA, except for histidine, isoleucine, leucine (Leu), phenylalanine, alanine (Ala), and glutamic acid (Glu). The AID of AA, except for Leu and Cys and the SID of AA, except for Leu, Ala, Glu, and Pro in SBM were greater (p < 0.05) than in corn. As the age of birds increased from 10 to 22 d, digestibility of all AA increased (p < 0.05), regardless of the expression of AA digestibility (i.e., AID and SID). In conclusion, the AID and SID of AA in both corn and SBM increased with increasing age, and the AID and SID of AA in SBM were greater than in corn.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권7호
• /
• pp.1020-1024
• /
• 2003

Five barrows (average initial body weight 58.6 kg) were used to determine the apparent ileal digestibilities of amino acids, DM, N and energy in various soybean meal, rapeseed meal and coconut meal in finishing pigs. Dietary treatments included 1) KSBM (Korean soybean meal), 2) CSBM (Chinese soybean meal), 3) ASBM (Argentine soybean meal), 4) RSM (Rapeseed meal), and 5) CNM (Coconut meal). The diets were corn starch-based and formulated so that each protein source provided the same amount of total ME (3,490 kcal/kg), CP (15.70%), lysine (1.00%), Ca (0.80%) and P (0.60%). Protein content of the KSBM was higher than the CSBM and ASBM, with all values similar to those expected, and protein content of the CNM was lower than that of the SBM preparation and RSM. Apparent ileal digestibilities of histidine, lysine, threonine, alanine, asparatic acid, cystine, glutamic acid and serine were greater for the KSBM, CSBM, ASBM and RSM than for the CNM (p<0.05). Also, the apparent ileal digestibilities of methionine, leucine, phenylalanine, valine and tyrosine were greater for the KSBM than for the CSBM, ASBM, RSM and CNM (p<0.05). Overall, the apparent ileal digestibilities of total essential amino acids were greater for the KSBM than for the CSBM, ASBM, RSM and CNM (p<0.05), and the apparent ileal digestibilities of total non essential amino acids were greater for the KSBM, CSBM, ASBM and RSM than for the CNM (p<0.05). No difference (p>0.05) in apparent digestibility of DM at the small intestine was observed among the treatments. However, the apparent digestibility of DM at the total tract was greater for the KSBM than for the CSBM, ASBM, RSM and CNM (p<0.05). Also, apparent digestibilities of N and digestible energy at the small intestine and total tract were greater for the KSBM than for the RSM and CNM (p<0.05). In conclusion, nutrient digestibility values of SBM preparations and RSM were relatively high compared to CNM.