• 대한바이러스학회지
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• 제29권1호
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• pp.1-9
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• 1999

Hantaan virus is widely distributed among rodent populations in Korea. Two antigenically distinct hantaviruses were isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. This study was designed to investigate the serological evidence of hantavirus infection among indegenous wild rodents, which were captured in 11 mountains located in Kyunggi, Kangwon, Chungnam, Chunbug and Kyungnam province of South Korea. A total 252 wild rodents of 3 species were trapped from Myungsung Mt., Chumbong Mt., Kali Mt., Hansuk Mt., Chachil peak, Kyebang Mt., Odae Mt., Kyerong Mt., Kaya Mt., Togju Mtand Chiri Mtin 1997. Serologic test for hantavirus infection was performed using hantavirus antigens by indirect immunofluorescent antibody techniqueAmong 122 Apodemus agrarius, 88 Apodemus peninsulae and 42 Eothenomys regulus; 18 A. agrarius (14.8%), 12 A. peninsulae (13.6%) and 4 E. regulus (9.5%) were immunofluorescent antibody (IFA) positive against hantaan virus. IFA titers 3 Eothenomys regulus sera were higher against puumalavirus than hantaan virus. These data imply that above three species of rodent might be natural reservoirs of hantaviruses in Korea.

• 대한바이러스학회지
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• 제30권1호
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• pp.19-28
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• 2000

Two distinct hantaviruses have been isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. Since our serosurveys conducted in 1994, a genetically distinct hantavirus from Apodemus peninsulae has been investigated. To isolate hantavirus from Apen insulae captured in Korea, the lung homogenate of seropositive Apeninsulae inoculated Vero E6 cells. Viral antigen was detected in a progressively higher percentage of cells with subsequent passage after 80 days postinoculation. The new isolate from seropositive Apodemus peninsulae was designated Suchong virus after Suchong valley located in northeastern region of South Korea. Comparing with hantaan virus 76-118 strain, Suchong virus-1, 2, 3 and 4 showed the similarity of $71.0{\sim}91.8%$ at nucleotide and $90.9{\sim}94.8%$ at amino acid sequences in 231 nucleotides region of M segment, and the similarity of $75.1{\sim}81.0%$ at nucleotide and $97.5{\sim}100%$ at amino acid sequences in 237 nucleotides of S segment.

• Animal Systematics, Evolution and Diversity
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• 제7권2호
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• pp.179-188
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• 1991

한국의 제주도에 서식하고 있는 등줄쥐의 한 아종인 Apodemus agrarius chejuensis의 분류학적 위치의 파악을 위하여, 아시아에 서식하는 등줄쥐중에 터키,중국,대만,만주 및 한국에서 채칩된 8아종(agrarius, ningpoensis, pallidior, chevrieri, insulaemus, manchuricus, coreae 및 chejuensis), 311마리의 표본들을 사용하였다. 4개 외부형질과 27개 두골형질들을 측정하였고 cluster, principal components 및 discriminant 분석등의 다변량분석을 수행하였다. 4개형(form)으로 나뉘어졌는데, chejuensis는 가장 큰 형이었고, 큰 형인 chevrieri는 Wang(1985)에 의하여 종으로도 기술된 바가 있다. 중간 크기의 형과 작은 크기의 형은 각각 coreae와 나머지 5개 아종(agrarius, ningpoensis, pallidior, in-sulaemus 및 manchuricus)이었으며, 연속적 변이를 나타냈다. 제주도의 chejuensis(가장 큰 형)는 등줄쥐의 다른 6개 아종(중간과 작은 크기의 형들)과 형태적 형질에 있어서 불연속적 차이를 보였으므로 신종으로 판단되지만, 신종으로서의 기재를 위해서는 연구가 더 필요하다고 결론지었다.

• 한국동물학회지
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• 제25권2호
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• pp.81-92
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• 1982

한국내 4개 지역에서 채집된 17마리의 등줄쥐 (Apodemus agrarius coreae)의 G-와 C-banding patter을 분석한 결과 첫 번째 염색체쌍의 다형현상 (차단부 및 단부)은 동원체 주위의 이질염색질이 어떤 때는 short arm의 형태로 나타나기 때문이라는 것이 밝혀졌다. 한국내 2개 지역에서 채집된 곰쥐 (R. rattus rufescens)의 G-와 C-banding pattern을 분석한 결과 첫 번째 염색체쌍의 다형현상 (차단부 및 단부)은 pericentric inversion에 기인한다는 것이 밝혀졌다.

• 한국동물학회지
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• 제34권1호
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• pp.59-63
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• 1991

Sperm morphology of ho species (hpodemus agrarius coreae and Apodemus peninsulae peninsulae) of the genus Apodrmus in Korea was investigated. Two species were similar in six qualitative characters, i. e., head shape, presence of apical ridge, process, cone-like process, and mitochondrial sheath, and the degree of development in outer dense Hber, Lengths of head and midpiece were not different with each other, but length of principal plus end piece was significantly larger (P ≪ 0.001) in hpodemus agrarius coreae than that in hpodemus peninsular peninsulae.

• Applied Microscopy
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• 제28권3호
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• pp.345-362
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• 1998

In order to the comparative morphological study of the non-ciliated and ciliated epithelial cells, and to elucidate the process of degeneration of non-ciliated epithelial cell of the ductus epididymidis, Korean striped field mouse, Apodemus agrarius coreae was examined with light and transmission electron microscopes. The morphological characteristics of non-ciliated epithelial cell, the cell types of the caput epididymidis (Cp), corpus epididymidis (Cr) and cauda epididymidis (Cu) were long-columnar, short-columnar and short-cuboudal, respectively. The mitochondria and rough endoplasmic reticulum tended to be broken as they immigrated from Cp to the Cu. The Golgi acted vigorously at the Cp, but the Golgi was inactive in Cr and Cu. The secretory vesicles and lysosomes were increased gradually from Cp to the Cu. The process of degeneration of the non-ciliated epithelial cells observed in the Cp, Cr and Cu epididymidis. The increase of the non-ciliated epithelial cells, and its degeneration were observed more often from Cp to the Cu. The morphological characteristics of the ciliated epithelial cells, the cell types of the Cp, Cr and Cu were long-columnar, short-columnar and short-cuboudal, respecptively like the non-ciliated epithelial cells. The stereocilia was long and slender at the Cp and Cr, while Cu was very short. The pinocytotic vesicles and absorptive vesicles were increased from the Cp to the Cu. Numerous disintergrated products was existed at the Cr including the Cp, but Cu were not observed. A significant amount of lysosomes existed at the Cp and Cr epithelial cells, but they were not observed in Cu epithelial cells.

• 대한바이러스학회지
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• 제28권4호
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• pp.337-345
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• 1998

Hantaviruses are distributed in rodent population world-widely even in geographical areas where hemorrhagic fever with renal syndrome (HFRS) has not been reported. Various species of Family Muridae and Arvicolidae serve as the natural reservoirs of hantaviruses. Hantaan virus, Seoul virus, Puumala virus, Prospect HII virus, Sin Nombre virus and New York virus are members of genus Hantavirus and isolated from lungs of A. agrarius, R. norvegicus, C. glareolus, M. pennsylvanicus, P. maniculatus and P. leucopus respectively. This experiment was intended to find the distribution of hantavirus infection among wild rodents and isolate the hantavirus from lung tissue of seropositve Apodemus peninsulae, and compared the nucleotide and amino acid sequences with prototype of hantaan virus 76-118 strain. Hantaviral sequences were amplified from lung tissues of A. peninsulae by reverse-transcriptase polymerase chain reaction. Alignment and comparison of the 324 nucleotide of G2 region of M-genomic segment diverged 4.6% and 0% at the nucleotide and amino acid levels, and complete N protein-coding region of S-genomic segment diverged 3.7% and 1.4% nucleotide and amino acid levels, respectively. This is the report to spill-over on the hantaan virus from A. agrarius to A. peninsulae in Korea.

• Animal Systematics, Evolution and Diversity
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• 제14권1호
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• pp.51-57
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• 1998

완도산 등줄쥐의 분류학적 위치를 재검토하기 위해, 한국의 8개 지역에서 채집한 등줄쥐(Apodemus agrarius)의 31개 형태적 형질들을 다변량분석방법으로 분석하였다. 두 군으로 나누어 졌는데, 6개 지역(태백산, 월악산, 청주, 지리산, 군산, 진도)의 작은 형과 2개 지역(완도, 제주도)의 큰 형이었다. 6개 지역의 작은 형은 Thomas(1908)가 밝힌 대로 A. a. coreae이고, 제주도 지역의 큰 형은 Johnson과 Jones(1955)이 이 기재한 대로 A. a. chejuensis임이 확이되었다. 뿐만 아니라, 완도의 큰형은 Joh(1989)가 제안한대로 A. a. chejuensis임도 확인되었다.

• 대한미생물학회지
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• 제19권1호
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• pp.1-10
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• 1984

The occurrence of Hemorrhagic fever with renal syndrome was noted for the first time among the United Nations troops in Korea in 1951. In 1976, Lee and Lee demonstrated for the first time an antigen in the lungs of a striped field mouse, Apodemus agrarius, which gave specific immunofluorescent reactions with sera from patients convalescent from Korean hemorrhagic feve (KHF). The natural reservoir host of KHF is Apodemus agrarius coreae mice in rural endemic areas in Korea. Clinical manifestations of acute illness do not occur in infected Apodemus agrarius. Infected rodents excrete large amounts of virus in saliva, in urine and in feces for a long period of time. In this study, the tissues of Apodemus agrarius, Wistar rat and Balb/C mouse were inoculated with Hantaan virus and subsequently observed for any histopathologic findings. 1. In lungs, infiltration of lymphocytes in alveolar septa and peribronchial region and thickening of and giant cell formation in alveolar septa were observed. 2. In kidney, vascular congestion, interstitial hemorrhage in corticomedullary junction and partial microscopic hemorrhage in urinary space were found. 3. In spleens, vascular congestion, old and recent hemorrhage and multinucleated giant cell formation were seen.

• 대한수의학회지
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• 제39권3호
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• pp.407-416
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• 1999

This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{\mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.