• 동의생리병리학회지
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• 제21권6호
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• pp.1520-1524
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• 2007

The purpose of this study was to investigate the effects of Houttuynia cordata Thunb and Oriental Herb Mixture Extract (HCTM) through anti-oxidation against the hepatotoxicity-inducing lipopolysaccharide (LPS) in HCTM and LPS-treated rats. HCTM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. On the day 21, 1.5 ml/kg of LPS was injected 5 hours before anesthetization. The activity of superoxide dismutase (SOD) was measured in mitochondrial fraction and malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx) were measured in liver homogenate. LPS-treatment markedly increased the levels of MDA and significantly decreased those of SOD, CAT and GPx. But HCTM pretreatment significantly increased those of SOD, CAT and GPx by 86.9%, 57.2% and 72.7% respectively. Moreover HCTM pretreatment decreased the levels of MDA. These results showed the HCTM had the effects against the hepatotoxicity-inducing LPS in the anti-oxidation. This suggested that HCTM could be used for functional beverage.

• 동아시아식생활학회지
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• 제26권4호
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• pp.359-369
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• 2016

In this study Ragwort, which had various and excellent pharmacological activity, was selected as a functional material, and intended to present an optimum condition of Ragwort Injeolmi by the blanching method, commonly used in the field. The general components, antioxidation, and dietary fiber of Ragwort were analyzed, and produceableness of Ragwort added Injeolmi was reviewed through instrumental inspection and sensory evaluation of various adding ratio of lyophilized Ragwort power and Ragwort pre-processed by blanching. It was proved that the SOD-likely active of Ragwort was 45.69%, total poly- phenol content was 12.45 mg, and total flavonoid content was 10.25 mg. The pH of Injeolmi adding Ragwort powder showed an increasing trend in the order of 0%, 1%, 3%, 5%, while that of Injeolmi adding blanching Ragwort showed increasing trend in the order of 0%, 10%, 15%, 20%, and there were significant difference (p<0.001). The soluble solid content of all sample groups showed constant value, and it was understood that no sugar was added in the process. From chromatography measurement result, a-value (redness) of 1% sample group was the lowest, and there were significant differences among sample groups (p<0.001). During 3 days of storage period, total microbial count of Injeolmi showed increasing trend while the storage period was getting longer. From the acceptability test Injeolmi adding blanching Ragwort had shown generally higher value.

• 한국동물생명공학회지
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• 제38권4호
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• pp.189-198
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• 2023

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2, Gsr, HO-1, and catalase, were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2, HO-1, and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

• 동의생리병리학회지
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• 제30권3호
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• pp.164-176
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• 2016

This study was perfomed to investigate the effects of Chunghyul-plus(CHP) on oxidative damage and hyperlipidemia in db/db mouse. After treatment with CHP, safety in cytotoxicity, heavy metal toxicity, production of reactive oxygen species(ROS), nitric oxide (N0) and proinflammatory cytokine IL-Ib, TNF-a, IL-6 in RAW 264.7 cells. Serum total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride, insulin, GLP-1, glucose, food intake, body weight, organ weight, AST, ALT, ALP, BUN, creatine and histologic change of liver and aorta were measured in db/db mouse after oral administration of CHP. CHP showed safety in cytotoxicity and toxicity of liver and kidney for logn time administration. CHP increased the DPPH and ABTS radical scavenging activity. CHP showed significant inhibitory effect on reactive oxygen species (ROS), and showed inhibitory effect on nitiric oxide(NO) compared to control group. CHP decreased cytokine IL-6 production significantly, and decreased IL-1β and TNF-α compared to control group. CHP decreased body and organ weitht, intake food, and glucose levels compared to control group. CHP decreased total cholesterol and triglyceride significantly, and decreased LDL-cholesterol levels and increased HDL-cholesterol levels compared to control group. CHP decreased atherogenic index and cardiac risk factor significantly. CHP increased serum insulin and GLP-1 compared to control group. In histologic examination, lipophagy in the liver and aorta decreased in CHP treated mice and the cell was regular and boundary of vessel wall was clear compared to control group. These results suggest that CHP is effective in antioxidation activity and treatment and prevention of hyperlipidemia, atherosclerosis, diabetes, ischemic heart disease, stroke and other cardiocerebrovascular disease.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1156-1166
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• 2020

Objective: The aim of this study was to evaluate the effects of compound organic acid calcium (COAC) on growth performance, hepatic antioxidant status and intestinal barrier of male broilers under high ambient temperature (32.7℃). Methods: Nine hundred healthy one-d-old Cobb-500 male broiler chicks were randomly assigned into three groups with six replicates of 50 birds each. A basal diet supplemented with 0% (control), 0.4% and 0.8% COAC, respectively were fed to birds for 6 weeks. All treatments were under high ambient indoor temperature of 32.7℃, and had a constant calcium and available phosphorus ratio. Results: The results showed that, compared with control, the average daily gain of broilers in 0.4% and 0.8% was significantly increased and the ratio of feed to gain in in 0.4% and 0.8% was significantly decreased at 1 to 21, 22 to 42 and 1 to 42 days of age (p<0.05). Compared with control, 0.8% COAC slightly decreased (p = 0.093) the content of malondialdehyde in liver at 42 days of age while 0.4% COAC significantly decreased (p<0.05) the activity of alkaline phosphatase. Furthermore, 0.4% COAC significantly enhanced the intestinal barrier function via increasing jejunal and ileal ocln transcription, promoting jejunal mucin 2 transcription at 42 days of age (p<0.05), and decreasing jejunal toll-like receptor 2 (TLR-2) and ileal TLR-15, inducible nitric oxide synthase compared with control group (p<0.05). Whereas, no significant differences on the transcription of interleukin-1β in jejunum and ileum were observed among three treatments (p>0.05). Overall, heat stress caused by high natural environment temperature may induce the damage to hepatic antioxidation and intestinal barrier. Conclusion: Dietary inclusion of COAC can improve the tolerance of broilers to thermal environment through the modification of antioxidative parameters in liver and the mRNA expression of genes in intestinal barrier, resulting in an optimal inclusion level of 0.4%.

• 한국축산식품학회지
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• 제37권2호
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• pp.181-190
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• 2017

The aim of this study was to compare the quality and physicochemical characteristics of blue-shelled eggs (BE) and conventional eggs (CE). Proximate composition, quality, pH value, shell color, collagen content, fatty acid composition, total cholesterol, ${\alpha}$-glucosidase inhibition activity, and antioxidation activity were determined. The proximate composition, general qualities, and pH values of CE and BE showed no significant differences, except in moisture composition, weight, and shell thickness. Moisture content and weight of BE were significantly lower than those of CE. However, shell thickness and weight of BE were higher than those of CE (p<0.05). Lightness of BE was significantly higher than that of CE (85.20 vs. 58.80), while redness ($a^*$) and yellowness ($b^*$) of BE were lower than those of CE ($a^*$: -4.75 vs. 14.20; $b*$: 10.45 vs. 30.63). The fatty acid [C18:1n7 (cis-vaccenic acid) and C18:3n6 (gamma-linolenic acid)] contents of BE were significantly higher than those of CE. The total cholesterol contents of BE and CE were similar. DPPH radical scavenging activity of BE was significantly higher than that of CE (40.78 vs. 35.35). Interestingly, ${\alpha}$-glucosidase inhibition activity of whole egg and egg yolk in BE (19.27 and 36.06) was significantly higher than that of whole egg and egg yolk in CE (13.95 and 32.46). This result indicated that BE could potentially be used as a functional food material. Further studies are required to evaluate the specific compounds that affect functional activity.

• 생명과학회지
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• 제14권3호
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• pp.411-416
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• 2004

홍조류의 하나인 서실을 추출, 분획하여 각 용매별 항산화와 항균효과를 관찰하였다. 서실의 항산화 효과를 측정하기 위해 DPPH radical 소거능과, $.$ $O^{2-}$$.$ scavenging activity를 측정함으로서 $.$ $O^{2-}$ 제거능을 실험하였다. 항산화 효과를 실험한 결과는 메탄을 분획층인 CCMM층이 대조군으로 사용한 일반적 항산화제인 BHT와 vitC와 유사한 DPPH radical 소거능을 보였고, $O^{2-}$ 제거능은 CCMM층과 CCMA층에서 활성을 보였다. 본 실험의 결과로 CCMM층에서 항산화 효과가 제일 좋았고 CCMA층에서도 항산화 효과를 가지는 물질이 있을 것으로 사료된다. paper disc method를 이용한 항균활성 효과 실험 결과, 홍조 해조류인 서실의 Bacillus subtilis를 제외하고는 CCMB층에서의 항균력이 가장 높게 나타났으며 실험 군주 4종 모두 CCMH층에서는 미약하나마 효과가 있었다. 본 실험 결과 홍조 해조류인 서실의 항산화 효과 검색에서 Methanol층인 CCMM층이 항산화 효과가 가장 좋았으며 항균효과 검색에서는 CCMB층이 활성을 나타내었다. 이 연구결과를 토대로 서실의 항산화 및 항균활성물질에 대찬 단계적인 분리 동정이 이루어져 CCMM층과 CCMB층의 생리활성물질 개발이 기대되는 바이다.

• 대한화장품학회지
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• 제38권2호
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• pp.155-162
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• 2012

숙성된 인삼은 인간의 건강을 회복시키고 향상시키는 강장제로서 알려져 있으며, 백삼보다 더 많은 약리적인 활성을 가진다고 알려져 왔다. 전이 가능한 종양, 림프구 증식과 쥐간의 지질과산화에 대한 숙성된 인삼의 효과도 연구되었다. 본 연구는 3가지 인삼 추출물의 항산화와 항노화 효과를 평가하기 위해서 실험하게 되었다. 시장에서 이미 만들어진 홍삼엑기스를 사용하였고, 그 외 수삼, 건삼을 구매하여 홍삼제조기로 만들어진 추출물로 실험에 사용하였다. 숙성된 인삼의 3가지 추출물들은 superoxide radical 소거 활성, hydroxyl radical 소거 활성, 리놀산 자동산화 억제, 섬유아세포에서 collagenase 억제와 collagen 합성의 효과를 연구했다. 숙성시켜 만든 수삼은 100 ug/mL의 농도에서 가장 높은 superoxide radical 소거 활성뿐만 아니라 vitamin C보다 더 높은 hydroxyl radical 소거 활성을 나타냈다. 또한 리놀산 자동산화 억제, collagen 합성과 collagenase 억제에서 가장 높은 활성을 보였다.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.214-218
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• 2009

Sweetpotato [Ipomoea batatas (L.) Lam] leaves are excellent source of low molecular weight antioxidants such as polyphenols, anthocyanins and carotenoids compared to other leafy vegetables. Endogenous antioxidants in sweetpotato help our bodies to prevent ageing, heart diseases and cancer. In this study, to develop the proper cultivars for the functional feed materials, we investigated the contents of anthocyanin, $\beta$-carotene, and polyphenols as well as DPPH radical scavenging activity in leaves of 14 different cultivars at the time of the harvest. They showed a diverse antioxidation activity. In DPPH radical scavenging activity, cultivars of Nanjing 9, Yulmi and Shinzami showed higher activity, whereas cv. Huiza 6 showed the lowest. Cultivars of Shinzami and Shinhwangmi had the highest anthocyanin (3.5 mg/g fr wt) and polyphenol (15.8 mg/g fr wt) content, respectively. Interestingly, there was a high correlation between cultivars with colorful pigments in storage roots and antioxidants activity in leaves. These results suggest that sweetpotato leaves with high antioxidant activity at harvest would be suitable for functional feed materials.

• 대한본초학회지
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• 제30권1호
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• pp.11-18
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• 2015

Objectives : The objective of this study is to evaluate antioxidant activity and the main component content change of steaming-drying fermented Gastrodiae rhizoma extract. Methods : The antioxidant activities were determined for total polyphenol, flavonoid contents, SOD-like activity, electron donating activity, nitrite scavenging ability and major functional components(gastrodin and 4-hydroxybenzyl alcohol content) were also measured. Results : The polyphenol content of fermented Gastrodiae rhizoma by S. cerevisiae were higher than those of fermented Gastrodiae rhizoma by A. oryzae, and when the fermentation period is extended. SOD like activity of fermented Gastrodiae rhizoma showed to be increased by fermentation with S. cerevisiae than fermentation by A. oryzae. Electron donating activity of fermented Gastrodiae rhizoma were increased at almost parallel level as vitamin C, by fermentation. Notably, fermentation by A. oryzae was moderately better than fermentation by S. cerevisiae. Flavonoid content of Gastrodiae rhizoma showed to increasing by fermentation, particularly fermentation by S. cerevisiae was proven to be more effective than by A. oryzae. The more steaming-drying or increased period of fermentation would be resulted in more gastrodin contents but under the same conditions, 4-hydroxybenzyl alcohol content of fermentation by A. oryzae in case of steaming and drying 1 time and 3 time was higher than control. Conclusions : These results has strongly hint the possible applicability of fermentation might be effective to improve the diverse biological activities of Gastrodiae rhizoma and may further supports to develop a functional food materials.