• 한국식품영양과학회지
• /
• 제28권3호
• /
• pp.526-533
• /
• 1999

Two species of antimicrobial agent producing bacteria and one sensitive strain were isolated from soil. Those were identified as B. subtilis, B. licheniformis, and Curtobacterium sp. by morphological, biochemical, physiological and chemotaxonomic characteristics. These were designated as B. subtilis cx1, B. licheniformis cy2 and Curtobacterium sp. cf3, respectively. Antimicrobial agent produced by B. subtilis cx1 showed high antibacterial activity against gram positive bacteria including of B. subtilis, Curtobacterium sp., L. mesenteroids, Staphy. aureus, S. faecalis and even gram negative bacteria, P. aeruginosa. Antimicrobial agent from B. licheniformis cy2 showed slightly lower antimi crobial activity than that from B. subtilis cx1. These two strains showed maximum production of antimicrobial agents at 30oC for 9~21hr cultivation. Curtobacterium sp. cf3 showed more sensitive activity than a sensitive strain of B. subtilis ATCC 6633 which was same genus or species with the B. subtilis cx1 and B. subtilis cy2, when the antimicrobial agent producing strains, B. subtilis cx1 and B. subtilis cy2, were directly applied onto these sensitive lawns.

• 대한수의학회지
• /
• 제24권2호
• /
• pp.149-162
• /
• 1984

The research was conducted(1) to confirm the agent(s) responsible for the antimicrobial activity contained in the fermented tomato juice with L. acidophilus(2) to extract and purify the antimicrobial agent(s)(3) to find the biological, physical and chemical properties of the agent(s). The following results were obtained and summarized as followings; 1. The agent responsible for the inhibitory activity was confirmed by both well assay method using fermented tomato juice with L. acidophilus and turbidimetric technique using the cell-free filtrate or neutralized filtrate of tomato acidohilus culture and found exerted antimicrobial agent other than lactic acid. 2. The procedures of purification : The isolation and purification of antimicrobial agent from the lyophilized acidophilus tomato culture were carried out by (1) methanol extraction (2) acetone extraction, (3) Sephadex G-50 gel filtration (4) paper chromatography and (5) thin layer chromatography. 3. The biological, physical and chemical properties of antimicrobial agent: The biological, physical, chemical properties of the purified antimicrobial agent were: (1) The antimicrobial activity was strong against test organisms; Bacillus subtilis(ATCC 6633), Escheichia coli(ATCC 25922), Staphylococcus aureus(ATCC 167), Pseudomonas fluorescens(KFCC 32394), Proteus vulgaris and Shigella dysenteriae. (2) The pH value of the agent was 2.0 and the inhibitory activity was lost when it was neutralized at 7.0 of pH and the agent was heat stable at $121^{\circ}C$ for 60 minutes. (3) The ultraviolet light absorption spectra of methanol-acetone extract and TLC fraction exhibited a maximum absorption at 260nm and 224nm respectively. (4) The most purified agent from TLC plate increased about 130-fold in activity. (5) The agent isolated from TLC plate was free from $H_2O_2$ or lactic acid. 4. Bioautographic assy: By means of bioautography of the agent on silica gel of TLC plate a strong inhibitory activity against B. subtilis was demonstrated. 5. Mass spectrometry: The agent obtained from TLC plate was analyzed by mass spectrometry which show the parent peak at m/e 264 suggesting the molecular weight of the compound and molecular group such as [$C_2H{^+}_4$], [CO], [CH=NH], [$C_3{H^}4_7$], [$\begin{array}{rcl}O\\{\parallel}\\CH_3-C\\\end{array}$], [$C_6-H{^+}_{11}$], [$C_5H{^+}_{11}$], [$\begin{array}{rcl}O\\{\parallel}\\C_5H_7-C^+\\\end{array}$] were suggested.

• 한국염색가공학회지
• /
• 제23권4호
• /
• pp.257-262
• /
• 2011

The dish cloth requires antimicrobial activity for hygienic purpose. Until now, organic antimicrobial agents of low cost have been frequently used for the anitimicrobial finishing. However, a cleaner hving anitimicroial activity should be more durable considering consumers' ever-increasing expectation. In this study, the cleaner containing the bamboo fiber were finished with a formulation of a new zirconium-containing inorganic agent of zirconium phosphate and polyurethane binders. The optimum treatment condition was investigated. And its durability against repeated laundering cycles was assessed. The existence of Zr after the repeated laundering was verified and the antimicrobial effect did not decrease under 30 washing cycles.

• KSBB Journal
• /
• 제16권2호
• /
• pp.123-127
• /
• 2001

항균 기능성 폴라스틱애 대한 향균력 시험방법 기준을 설 정하가 워하여 E. coli 및 S. aureus를 공시균주로 하여 균주 별 항균제 Ag-hydroxyl aphatite의 첨가량에 따른 플라스탁회 항균력을 비교실험하였다. 첫번째 평카방법 기준올 설정하기 위해 콩시균외 최적 활성농도 범위를 설정하였고, 두 번째 이를 기준으로 한 시힘용 플라스틱의 지준 시편을 제작하여 항균성능 비교를 통한 시험방볍을 검토하였다. 실험결과 균 캡종용액으로 사용한 균 용액의 농도범위는 Trγpticase Soy Broth흘 0.1 M 멸균 인산완충용액으로 500배 희석한 첨가배지가 적합하였고, 처리조건으로 $37\pm1^{circ}C$, 120 rpm, 24시간동안 항온 진탕 시험하여 균 용액의 사용범워를 $4~6\times10^5$CFU/mL이었을 때가 항균 활성효과 시험에 가장 적합하였다. 그리고 항균제의 첨가군에 따른 플라스틱 종류별 항균성능은 동일한 첨가함량의 경우 비슷한 항균효과를 보였으며 기준시료의 항균제 첨가효과 적용범위는 0.5 % (w/w) 이상의 시험편을 작용하는 것이 항균력 평가 결과 균 감소량에 재현성 있는 적용범위 선정이 가능함을 알 수 있었다.

• 한국염색가공학회지
• /
• 제15권6호
• /
• pp.47-54
• /
• 2003

To be dyed with cationic Berberine, the cotton fiber was modified with reactive anionic agent. The Berberine, a natural cationic colorant of pure yellow, is the major component of Amur Cork tree extract and also can be employed as a natural antimicrobial agent due to its characteristic of cationic quaternary ammonium salt. By LC/MS analysis, it became obvious that the Berberine was contained in Amur Cork tree extract as a major color component. The adsorption of the Berberine on the cotton fabrics pretreated with the anionic agent was greatly increased comparing to that of untreated fabric. Because the anionic agent was colorless, it did not cause unintended color change of the dyeings. The dyed fabric with the Berberine has strong antimicrobial activity showing 99.5% of reduction of bacteria against Staphylococcu aureus.

• 한국염색가공학회지
• /
• 제15권6호
• /
• pp.27-32
• /
• 2003

As a natural antimicrobial agent, gallnut extract was examined. The extract was separated and analyzed by LC/MS to assign the components, gallic acid, digallic acid, trigallic acid, mono-galloyl glucose, penta-galloyl glucose, etc. Minimum inhibitory concentration of gallnut extract was about 10ppm. Cotton fabrics treated with 10% owf of gallnut extracts had antimicrobial activity showing the reduction of bacteria 99.9% for both Staphylococcus aureus and Klebsiella pneumoniae.

• 자연과학논문집
• /
• 제11권1호
• /
• pp.159-164
• /
• 1999

유기실리콘 제4급 암모늄염을 이용하여 타올에 향균제를 처리하였을 때의 향균성에 가장 많은 영향을 미치는 처리농도, 시간, 온도에 대한 향균성 변화를 비교 조사하였고, 향균처리 후 물성변화, 내세탁성을 측정하였다. 타올의 향균성에 가장 우수한 향균성을 나타내고 많은 영향을 미치는 처리농도는 3.5%이며, 처리온도 $30^{\circ}C$에서, 처리시간 10분간 향균가공 처리하여도 균감소율이 95% 이상 향균성능을 유지함을 알 수 있었다. 또한 세탁 횟수에 따른 균 감소율은 20회까지 세탁하여도 80%이상 높은 향균성을 나타내었고, 향균제 가공처리 후 측정한 타올의 인장강도 및 신도는 큰 변화가 없었다.

• 한국식품저장유통학회지
• /
• 제10권1호
• /
• pp.41-46
• /
• 2003

본 실험에서는 가공 두부를 천연항균소재인 자몽종자추출 물의 유도체에 침지 처리한 후 꺼내어 상온에서 저장하면서 가공 두부의 품질(총균수, 수분 함량, 조지방의 함량, 조단백의 함량, 표면색도)변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 천연항균소재의 희석액에 침지처리한 가공두부는 대조구에 비하여 수분함량, 조단백 및 조지방 함량이 높게 나타났으며, 총균수는 천연항균소재 처리구의 증가폭이 무처리 대조구에 비해 감소하는 경향으로 나타났으며 대장균수도 압도적으로 낮은 간을 보여 주었다. 아울러, 처리구의 표면색도는 대조구에 비하여 백색도를 두드러지게 나타내었다. 이와 같은 결과로 볼 때, 천연항균소재의 침지처리는 가공두부의 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다.

• 한국응용과학기술학회지
• /
• 제30권1호
• /
• pp.96-115
• /
• 2013

본 연구는 항균성 코팅제의 개념과 동향파악으로 항균성 코팅제의 연구개발의 방향을 설정하는데 도움을 주기 위한 것이다. 항균제는 미생물을 제거하거나 성장을 저지하는데 사용되는 화합물이며 항균 코팅제에 함유되는 항균제용 재료는 무기물, 금속, 저분자 유기물, 천연유기물, 고분자가 있다. 항균코팅제는 생활용품, 병원용품, 산업용품, 전자제품, 의류, 건축 내장재 등의 표면의 항균성 부여에 쓰인다. 기존 항생제는 세균의 세포벽을 손상하지 않고 미생물을 침투하나 항균성 고분자는 세포막을 파괴하므로 항생제의 내성을 방지할 수 있다. 대부분의 고분자 항균제는 양이온 고분자에 초점을 맞추고 있다. 항균제의 분자설계와 코팅제 배합의 합리화로 항균제의 선택성, 내구성, 독성 문제가 개선될 것이다.

• Archives of Pharmacal Research
• /
• 제12권2호
• /
• pp.119-124
• /
• 1989

Cotton xanthate, which was obtained by treating cotton with carbon disulfide in alkaline solution, was treated with the solution of polyvalent metal ions to produce cotton xanthate-metal chelates. This chelation reaction was readily and simply achieved, and antimicrobial agents with suitable structures could subsequently be coupled to the chelate with ease at moderate pH values and in aqueous solution. Metal ions used in present work include Cu(II), Zn(II) and Fe(III). Tetracycline, streptomycin, neomycin and pyrithion were used as antimicrobial/antifungal agents. Antibacterial activities were measured employing ditch plate method against G(+) Staphylococcus aureus, Streptococus faecalis, and G(-) Escherichia coli, Enterobacter aerogenes, and the fungus, Aspergillus niger. All the cotton xanthate-metal-antimicrobial agent chelates exhibited activities whereas the cotton xanthate-metal chelates themselves were inactive. Considering the extensive washing procedures and results from control experiments, possibility of the involvement of physical adsorption for the binding of drugs could be excluded.