• 한국의류산업학회지
• /
• 제15권4호
• /
• pp.630-634
• /
• 2013

Cow leather coated with polyurethane film that contains various organic photosensitizers was investigated to demonstrate the antimicrobial properties in the application of the material to protective clothing and home appliances. To prepare the antimicrobial coating on leather surfaces with high potency against microbes, photoactive agents, such as benzophenone (BP), 4,4'-bis(dimethylamino) benzophenone (MK), 4,4'-dihydroxybenzophenone (DHBP) and methylene blue (MB), were incorporated into polyurethane-based coating solutions. The photoactive antimicrobial agent treated leather samples were characterized by SEM, color appearance, color fastness against abrasion, and antimicrobial tests. The optical properties of organic photosensitizers indicated that active UV absorbance ranges were different: BP (around 250 nm), MK (around 360 nm), DHBP (around 305 nm) and MB (around 295 nm &570 nm-685 nm). The intensity of the UV absorbance curve at the UVA light wavelength for the antimicrobial test showed the highest value with MK; subsequently, this was followed by MB, DHBP and BP in decreasing order. The treated-leather samples demonstrated excellent antibacterial activity under UVA light. The antimicrobial effects for the Staphylococcus aureus were superior to Escherichia coli. Moreover, the polyurethane finishing showed an effective durability to abrasion. The overall results indicated that DHBP is the most suitable PU coating additive to provide antimicrobial properties to leather as well as color and surface appearance than MK, MB, and BP.

• 약학회지
• /
• 제38권6호
• /
• pp.742-748
• /
• 1994

As part of our search for less toxic antimicrobial agents from natural resources, the carpophores of Elfvingia applanata$(P_{ers}.)K_{ARST}.$ was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration(MIC). Among fourteen species of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.250 mg/ml. To investigate the effect of antimicrobial combinations of EA with four kinds of antibiotics(ampicillin, cefazolin, oxytetracycline and chloramphenicol), the fractional inhibitory concentration index(FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with four kinds of antibiotics resulted in synergism in four instances, but no antagonism was observed. Four instances of synergism were observed when EA was combined with ampicillin against Micrococcus luteus, with cefazolin against Bacillus subtilis, with cefazolin against Micrococcus luteus and with oxytetracycline against Staphylococcus aureus.

• KSBB Journal
• /
• 제17권4호
• /
• pp.345-349
• /
• 2002

항균성 물질이 함유된 투명한 신기능 포장필름의 제조를 위하여 흡착제로서 수용성 silica를 선택하여 항균성 물질의 흡착 및 내열성을 조사하였다. Methanol 자화 방선균 (MO-16, MO-17)이 생산하는 천연 항균성 물질을 ethylacetate로 추출, 농축하여 silica에 흡착시킨 후 열처리한 결과 15$0^{\circ}C$에서 5분까지 항균효과가 유지되었다. 따라서 투명필름의 제조시에 흡착제로서 silica의 첨가가 효과적이라고 판단되었다. 항균제인 benzoic acid(BA) 는 세균 및 곰팡이에 강한 항균효과를 나타내었으며, 특히 곰팡이에 대한 항균력이 우수하였고, 10$0^{\circ}C$, 5분간 열처리시에도 항균력을 유지하였다. 또한 항균성 물질과 공동 첨가시 약간의 상승효과를 나타내어 필름 제조시 공동 첨가하므로서 첨가량의 감소에 따른 비용의 절감 및 항진균 효과가 보완된 필름제조가 가능하다고 사료되었다.

• 한국식품영양과학회지
• /
• 제28권1호
• /
• pp.100-106
• /
• 1999

Use of chemical preservative for controlling harmful microorganisms in food products has been debated due to public concerns about food quality because of perceived toxic and carcinogenic potential. Thus, use of non toxic natural antimicrobial agents has become essential. This study was investigated to determine the antimicrobial activity of water or ethanol extract of commercially available tea, and of solvent fractionated ethanol extracts obtained from steamed green tea. Both of water and ethanol extracts of green tea(steamed or roasted), oolong tea and black tea exhibited strong antimicrobial activity against gram positive and negative bacteria, but not effective against yeast and mold. Also, antimicrobial activity of ethanol extract of 4 different kinds of tea was stronger than that of water extract. Among 4 different tea, ethanol extract of steamed green tea was further fractionated. One thousand g/disk buthanol extract had the strongest antimicrobial activity against bacteria and mold. The concentration of the antimicrobial activity of buthanol extract in tested microorganisms ranged from 125~1000 g/disk except for Rhizopus javanicus. Antimicrobial activity of buthanol extract of steamed green tea was not destroyed by heating at 100oC for 60 min and at 121oC for 15 min, which is very stable over heat treatment. The inhibitory effect of the buthanol extract on the growth of Listeria monocytogenes and Staphylococcus aureus was investigated. Growth of both strains was started in the presence of 250 and 500 g/ml after 12 and 24 hour respectively, whereas complete inactivation of both strains was occurred in the presence of 1000 g/ml.

• 한국식품저장유통학회지
• /
• 제1권1호
• /
• pp.1-7
• /
• 1994

To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.

• 한국식품영양과학회지
• /
• 제32권5호
• /
• pp.745-751
• /
• 2003

밀 단백질에 효소가수 분해할 때 생산되는 peptide의 항균활성과 천연항균제로서의 이용가능성을 검토하기 위하여 실험을 행하였다. 밀 단백질에 7종의 단백질가수분해효소를 작용시켜 생성된 가수분해물의 항균활성을 측정하고 한외여과, membrane filtration, HPLC를 이용하여 항균성 peptide를 분리 정제한 후 분자량과 아미노산 결합순서를 측정한 결과는 다음과 같다. 밀 단백질에 7종의 단백질 분해효소를 적용시켜 제조한 가수분해물중 Asp. saito protease를 적용시켜 얻어진 peptide만이 항균활성을 나타내었다. Asp. saito protease는 37$^{\circ}C$, pH 6.0에서 작용시킨 경우에 항균활성이 가장 높았으며, 5$0^{\circ}C$ 이상에서는 활성을 나타내지 않았다. 밀단백 효소가수분해물은 membrane filtration에 의하여 분자량 1,000~3,000에서 항균활성이 나타났다. Membrane filtration으로 얻어진 항균활성분획을 HPLC로 분리한 결과 retention time 31.1~31.8 min에서 항균활성을 나타내었다. 밀단백 효소가수분해물은 121$^{\circ}C$에서 15분간 가열하여도 효소활성이 유지되는 매우 안정한 화합물이었다. 항균활성분획을 MALDI-mass로 질량을 분석한 결과 1,633이었다. 항균성 peptide의 아미노산 결합순서는 cysteine, glycine, prolin, valine, valine, alanine, alanine, arginine의 순서였다.

• 대한한의학회지
• /
• 제41권2호
• /
• pp.1-8
• /
• 2020

Objectives: To establish the expiration date of herbal decoction pouch, the change in antimicrobial activities was measured according to storage temperature and period. Methods: Five herbal formulas, Gyeji-tang, Jakyakgamcho-tang, Saengmaek-san, Paljung-san, and Dangguisu-san, with antimicrobial activity were selected from 51 herbal formulas commonly used in traditional Korean medical institutions. Five herbal formula decoction pouches were prepared and stored at room temperature (RT) or 4℃ from 0 to 12 weeks to evaluate the antimicrobial activity against Bacillus cereus, Listeria monocytogenes, or Vibrio parahaemolyticus. The antimicrobial activities were evaluated by the disk diffusion method (disk size: 10 mm). Results: The decoction pouches of Gyeji-tang and Jakyakgamcho-tang maintained antimicrobial activity against V. parahaemolyticus from week 0 (14 mm) to week 12 (14 mm) at both temperatures. Saengmaek-san pouch had antimicrobial activities against V. parahaemolyticus and B. cereus from week 0 (19 mm and 20 mm) to week 12 (both 18 mm), respectively. At RT and 4℃, the antimicrobial activity of Paljung-san pouch was maintained week 0 (17 mm) to week 12 (both 13 mm) against B. cereus. Against L. monocytogenes, the antimicrobial activity of Dangguisu-san pouch showed 27 mm at week 0 and 23 mm (RT) and 24 mm (4℃) at week 12, respectively. Conclusion: According to increase of the storage duration, the antimicrobial activities of 5 herbal formula decoction pouches decreased slightly but remained up to 12 weeks. These results can be used as important data to establish the expiration date of herbal formula decoction pouches.

• 한국염색가공학회지
• /
• 제23권4호
• /
• pp.257-262
• /
• 2011

The dish cloth requires antimicrobial activity for hygienic purpose. Until now, organic antimicrobial agents of low cost have been frequently used for the anitimicrobial finishing. However, a cleaner hving anitimicroial activity should be more durable considering consumers' ever-increasing expectation. In this study, the cleaner containing the bamboo fiber were finished with a formulation of a new zirconium-containing inorganic agent of zirconium phosphate and polyurethane binders. The optimum treatment condition was investigated. And its durability against repeated laundering cycles was assessed. The existence of Zr after the repeated laundering was verified and the antimicrobial effect did not decrease under 30 washing cycles.

• 한국미생물·생명공학회지
• /
• 제27권5호
• /
• pp.391-398
• /
• 1999

A methylotrophic actinomycetes strain MO-16, which produce the antimicrobial compound, was isolated from soil and supposed as Amycolatopsis sp. based on taxonomic studies. The cell-free extract of methanol-grown strain MO-16 showed dehydrogenase activity for methanol and formaldehyde when various electron acceptors were added for oxidation. On the other hand, methanol did not affect the production of antimicrobial compounds, and organic nitrogen sources such as corn steep liquor and peptone were better than inorganic nitrogen sources. These compounds showed broad antimicrobial spectrum to the tested strains such as bacteria and yeast. The antimicrobial comounds were very stable under heat(121$^{\circ}C$), acid(pH2.0), alkali(pH11.0) treatments. These compounds were isolated by ethylacetate extract, silica gel column chromatography and reverse phase HPLC. Two compounds(peak 1 and 2) were detected as antimicrobial compounds through the HPLC analysis. The peak 2 was purified as a single compound and revealed a 98% purity.

• 한국임상수의학회지
• /
• 제34권6호
• /
• pp.425-428
• /
• 2017

Silver nanoparticles have marked antimicrobial effects on several pathogens and have been used to control bacterial growth in humans. In the present study, we evaluated the antimicrobial efficacy of silver nanoparticles against the common causative pathogens of canine otitis external through counting of colony forming units. Silver nanoparticles showed significant dose-dependent antimicrobial effects on pathogens. In addition, we conducted antimicrobial susceptibility tests and compared the antimicrobial efficacy of silver nanoparticles. Microorganisms with a high resistance to antibiotics were also resistant silver nanoparticle with low concentration ($5{\mu}g/mL$). However, in high concentration ($15{\mu}g/mL$), almost 100% reduction in the number of CFUs of these pathogens was observed.