• Title/Summary/Keyword: Antibody titers

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Relationship of Antibodies in Egg Yolk and Serum against Major Antigens of Bacterial Agents in Porcine Atrophic Rhinitis, Pneumonic Pasteurellosis and Pleuropneumonia (돼지 위축성 비염, 파스튜렐라성 폐렴 및 흉막폐렴 원인균의 주요 항원에 대한 IgG 와 IgY 의 상관 관계 분석)

  • Shin, Na-Ri;Kim, Jong-Man;Yoo, Han-Sang
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.371-376
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    • 2002
  • Swine respiratory diseases have induced severe economic lasses in swine industry worldwide. Therefore, several methods have been made and applied to prevent and control the diseases. However, these methods still have a problem and also induce side effects. Recently, the use of egg yolk antibody was introduced to control and prevent the diseases as one of new trials. As a study of using egg yolk antibody, antibody titers against several different antigens of major pathogens in swine respiratory diseases were compared in egg yolk and serum of hens immunized with those antigens. The titers were measured by ELISA using the antigens as coating antigens. The relationship in antibody titers between egg yolk and serum were identified by analysis of variance for linear regression. Almost of antigens used in this study showed the high relationship in antibody titers between egg yolk and serum (r = 0.87 ~ 0.93) even though the relationship in antibody titers against P. multocida A:3 IROMP was slightly low (r = 0.74)(P<0.01). These results indicated that antibody titer in egg yolk could be useful to predict the titer in serum of chicken.

Diagnostic Significance of Cold Agglutinin and Antimycoplasma Antibody for Mycoplasma pneumoniae Infection (항Mycoplasma 항체와 한냉응집소에 의한 Mycoplasma pneumoniae의 진단학적 의의)

  • Kim, Chung-Sook;Lee, Chae-Hoon;Jeon, Chang-Ho;Bae, Eun-Kyung;Hong, Seak-Il
    • Journal of Yeungnam Medical Science
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    • v.4 no.1
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    • pp.97-103
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    • 1987
  • A Study to evaluate the diagnostic significance of M. pneumoniae infection by measurements of cold agglutinin and antimycoplasma antibody titers is performed with 191 pediatric patients who have visited Yeungnam University Hospital during the period through January to July, 1987. Forty eight of 191 cases made follow up tests feasible. The results obtained are as follows : 1. It is necessary to perform routine combined measurements of cold agglutinin and antimycoplasma antibody titers for the all pediatric pneumoniae caser since a large proportion of pneumonia in children is caused by M. pneumoniae. 2. For the diagnosis of M. pneumoniae infection, measurements of cold agglutinin titers alone seems to be les significant than to check both cold agglutinin and antimycoplasma antibody titers. 3. The measurement of antimycoplasma antibody titer appeared to be more specific than cold agglutinin test in the diagnosis of M. pneumoniae infection. 4. The present study urges the necessity of follow up study of cold agglutinin and antimycoplasma antibody titers for those who initially presented with normal titers in both tests, but are clinically suspected for M. pneumoniae infection.

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Distribution of the serum Ig G titers to whole cell and leukotoxin of Mannheimia haemolytica Al in Holstein and Korean indigenous cattle slaughtered in abattoir

  • Byun, Jae-Won;Kim, Kyung-Ho;Lee, Sung-Mo;Lee, Jung-In;Hwang, Hyun-Soon;Kim, Yong-Hee
    • Korean Journal of Veterinary Service
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    • v.26 no.4
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    • pp.323-328
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    • 2003
  • A total of 419 slaughtered cattle were used to investigate the serum Ig G titers to the Mannheimia haemolytica Al whole cell and leukotoxin recognized with important virulence factor in bacterial pathogenesis. Data obtained in this study were represented with average absorbance${\pm}$standard deviation. Serum Ig G titers were detected with the ranges from 0.1 to 0.5 at 490nm. Whole cell titers were higher than leukotoxin antibody on the whole. Antibody titers of slaughtered cattle between races, ages have no significant difference but gradual decrease under aging in dairy cow for whole cell (decline mean titer from 0.29 to 0.27 according to age) was undertaken. Holstein bulls shipped from Seoul province had a significantly lower Ig G titers than those from another ones (p<0.05).

Investigation of Antibody Titers after Inoculation with Commercial Equine Influenza Vaccines in Thoroughbred Yearlings (Thoroughbred 1세말에서 상업용 말 인플루엔자 백신접종 후 항체역가 추적)

  • Yang, J.H.;Park, Y.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.1
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    • pp.89-96
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    • 2018
  • The object of this study was to evaluate the change of antibody titers on virus strains after inoculation with commercial killed equine influenza (EI) vaccines in horses. Serum antibodies of 20 Thoroughbred yearlings were detected using hemagglutination inhibition test for 41 weeks. Second vaccination is inoculated 4 weeks after the initial vaccination. Most of antibody titers were not increased until 4 weeks after first vaccination. The highest titers were detected 6-10 weeks after vaccination. The titers were decreased slowly and maintained for 16 weeks after inoculation. We could barely detect the antibody 41 weeks after vaccination in most cases. Vaccine anergia were appeared in 3 horses (15%) but it depended on virus strains. A/Equine/La Plata/93(H3N8) strain that induce high and durable antibody responses was the most effective among three strains. This study presents the first comprehensive data on the endurance of antibody titers against EI. Our data also suggests that yearlings should be inoculated three times in order to maintaining optimal antibody titers against EI. We speculate the causes of anergia were vaccine break down or individual specificity. Further research is needed to investigate immunological unresponsiveness. This was the first study on strain of equine vaccine in Korea.

The variation of serological titers on the chickens infected pullorum disease from Kyongbuk provinces (경북지방유래 추백리 양성계에서의 균분리 및 혈청역가 추이)

  • 김영환;김경희;우용구;장영술;조민희;김수웅
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.19-26
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    • 1997
  • The present study was conducted to investigate the general epidemiological situations with 18-pullorum infected chickens from Kyongbuk provinces during the period from June 1995 to January 1996. On the Salmonella pullorum isolation tests by rectal swab culture method from infected chickens (386-samples), any Salmonella spp was not isolated from infected live-birds. But 2-S pullorum were isolated of 2-dead chickens(33.3% ) from 6-dead chickens which were positively reacted by serological tests. On the other hand, we could not isolated any Salmonella spp. in any parts of egg-contents ; egg-shell, egg-white and egg-yolks with 25-infected bird eggs. On the tests of antibiogram, 2-S pullorum strains were highly sensitive to GM, AM, SXT, CZ, K, FIM, ENR, C, AN, N, NN, LIN+SP, CF, TE and PB, respectively and intermediate sensitive to the CB, CFP, CL, S, P and XNL. But 2-strains were resistant to CC, DP, E, L, OX, TLA and TyLO. In the serological tests, pullorum antibody titers of 18-infected birds was from 2.76 to 9.18 with average by the microplate test. During the 6-months, pullorum antibody average titers were not changed generally. To validate the effects of the antimicrobial agent treatments to the serological antibody titers, infected 6-chickens was medicated with 0.5%-futazolidone. The titer of premeditated birds was average 4.26 but after medication with furazolidone, the titers of treated 6-birds was average 4.08.

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Antigenemia and Specific IgM and IgG Antibody Responses in Rabbits Infected with Toxoplasma gondii

  • Quan, Juan Hua;Hassan, Hassan Ahmed;Cha, Guang-Ho;Shin, Dae-Whan;Lee, Young-Ha
    • Parasites, Hosts and Diseases
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    • v.47 no.4
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    • pp.409-412
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    • 2009
  • In this experiment, the correlation between antigenemia and specific antibody responses in Toxoplasma gondii-infected rabbits was assessed. We injected 1,000 T. gondii tachyzoites (RH) subcutaneously into 5 rabbits. Parasitemia, circulating antigens, and IgM and IgG antibody titers in blood were tested by ELISA and immunoblot. For detection of parasitemia, mice were injected with blood from rabbits infected with T. gondii and mice died between days 2 and 10 post-infection (PI). Circulating antigens were detected early on day 2 PI, and the titers increased from day 4 PI and peaked on day 12 PI. Anti-Toxoplasma IgM antibody titers increased on day 6 PI and peaked on days 14-16 PI. IgG was detected from day 10 PI, and the titers increased continuously during the experiment. The antigenic protein patterns differed during the infection period, and the number of bands increased with ongoing infection by the immunoblot analysis. These result indicated that Toxoplasma circulating antigens during acute toxoplasmosis are closely related to the presence of parasites in blood. Also, the circulating antigen levels were closely correlated with IgM titers, but not with IgG titers. Therefore, co-detection of circulating antigens with IgM antibodies may improve the reliability of the diagnosis of acute toxoplasmosis.

Shigella Antibody Titers in Korean with or Without Diarrhea (한국인(韓國人)의 건강인(健康人) 및 설사환자(泄瀉患者)의 Shigella 항체가(抗體價))

  • Ha, Tai-You;Chung, Sun-Sik
    • The Journal of the Korean Society for Microbiology
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    • v.3 no.1
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    • pp.25-30
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    • 1968
  • Shigella antibodies in 50 sera from healthy persons and 110 sera from patients with diarrhea were tested using microdetermination of the indirect bacterial hemagglutination with the polyvalent antigen, and the following results were obtained. A survey of sera collected from healthy persons revealed that 4% had positive titers, 1:64 or above, to Shig. flexneri, Shig. dysenteriae, and Shig. boydii, respectively, whereas all subjects were negative for Shig. sonnei, less than 1:64. Namely, 6 cases among the 50 subjects were positive. Among the patients with diarrhea, positive antibody titers were demonstrated in 29.9% against Shig. flexneri, 11.9% against Shig. boydii, 7.2% against Shig. dysenteriae, and 6.4% aginst Shig. sonnei, respectively. Therfoe, the total positive cases were 55.4% among 110 subjects. No correlation between Shigella and Salmonella antibody titers among patients with dirrhea was found.

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Protective Immune Reponses Induced by Non-infectious L-particles of Equine Herpesvirus Type-1: Implication of Cellular Immunity

  • Mohd Lila Mohd Azmi;Field, Hugh-John;Frazer Rixon;Lauchlan, John-Mc
    • Journal of Microbiology
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    • v.40 no.1
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    • pp.11-19
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    • 2002
  • Mice immunized with equine herpesvirus type-1(EHV-1) L-particles skewed a significant increase (p<7.75) in serum antibody titers. Upon a booster dose four weeks lateral antibody titers increased significantly. Interestingly, immunization via intravenous or intramuscular route induced significantly higher (p<0.75) antibody titers. However, mice iummunized with UV-treated L-particles, visions or immunization via intranasal route induced lower antibody titers. Upon challenge inoculation with wildtype EHV-1, our data showed there was a poor correlation between antibody titers and protection against virus replication. Therefore, the role of cell-mediated immunity Inwards protection was investigated. As predicted, the strongest cell-mediated immunity, as measured by delayed-hypersensitivity test, was detected in mice immunized with live virus particles. The magnitude of cell-mediated immune response correlated with the efficacy of L-particles as immunizing agent. The highest efficacy, as indicated in mice immunized via intranasal routed was highly correlated with cell-mediated immunity. A similar phenomenon was also demonstrated in mice immunized intranasally with UV-treated L-particles. However, the degree of protection was reduced when mice immunized intravenously or intramuscularly with UV-treated L-particles. In conclusion, protection conferred in these animals was highly implicated by immune cells and the least by antibodies. The route of immunization and the nature of the antigen also contributed to the efficacy of L-particles as immunizing agent. In contrast to that of herpes simplex virus type 1, our data showed EHV-1 non-infectious L-particles are highly suitable for immunization of the host against EHV-1 disease.

Immune Status of Breeding Hens Against Newcastle Disease (종계군(種鷄群)의 Newcastle Disease에 대(對)한 면역상태(免疫狀態)에 관(關)한 연구(硏究))

  • Yeo, Sang Geon;Choi, Won Pil
    • Korean Journal of Veterinary Research
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    • v.19 no.1
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    • pp.45-51
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    • 1979
  • The present study was undertaken to observe the immune status of breeding hens and laying hens against Newcastle disease (ND). The methods of extraction of hemagglutination inhibition (HI) antibody from egg yolk, the detection of HI antibody in egg albumen and the correlation between HI antibody titers in maternal sera and egg yolks were discussed. For the purposes of these experiments, 9 flocks of breeding hens and 16 flocks of laying hens immunized against Newcastle disease virus were investigated. The vaccination program of tested flocks was 3-3-3 or 4-4-4 in general. The results obtained are summerized as follows: Freezing-thawing was the best method far antibody extraction from egg yolk for HI test. The HI antibody against NDV was found in egg albumen (geometric mean, 4.5), but lower than that found in egg yolk (32.1). The geometric mean of HI antibody titers of egg yolks (84.1) was higher than that of maternal sera (68.4) and day-old chicken sera (25.3). There was correlation between HI antibody titers of maternal sera(Y) and those of egg yolks(X). The coefficient correlation was r=0.63, and the line of regression of Y on X was $\hat{Y}$=35.91+0.35X.

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Research of Strangles Antibody Titer of Horses in Jeju (제주에서 사육되는 말의 선역항체가 조사)

  • Lee, Young-woo;Jeong, Hyeon-woo;Lee, Kyoung-kap
    • Journal of Veterinary Clinics
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    • v.34 no.2
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    • pp.132-134
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    • 2017
  • Streptococcus equi subsp. equi which is a Gram-positive bacteria causes strangles a highly contagious disease that affects the respiratory system of the horse. The disease is characterized by fever, nasal discharges and enlargement of the lymph nodes in the head and upper neck region. This study was performed to monitor the level of serum strangles antibody titers of horses in Jeju. A total of clinically healthy 122 horses (41 horses less than 5 months, 81 horses more than 1 years old) were included in this study. Serum antibody titers against strangles were measured by means of Passive haemagglutination (PHA) titers. As the results, the level of foals was definitely low. The results of this study have shown that there is a need to consider the vaccination against strangles infection at foals.