• 한국동물위생학회지
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• 제31권3호
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• pp.315-329
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• 2008

A total of 710 bovine serum samples which are composed 532 bovine serum samples showed negative reaction and 178 bovine serum samples showed positive reaction with tube agglutination test (TAT) from North area of Gyeong-nam, Korea were tested using all the 3 assays which are Rose-Bengal test(RBT), tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA, two types) and analyzed for evaluation of specificity, sensitivity, reproducibility and predictive value. In the comparison of serum antibody titer agglutination test, RBT showed almost agreement with TAT. In the comparison of TAT and two types of ELISA method, they showed difference in specificity and sensitivity about 5%. But there is no significant difference in detecting sensitivity between two types of ELISA method and TAT. In serologic tests for bovine brucellosis, the new assay ELISA would be a good candidate for serologic survey for bovine brucellosis in Korea because it is efficient in detecting many test samples quickly. But the serum agglutination tests (RBT, TAT) are more economical and easy assay for detection. In the test of comparison of antibody titer between first day of finding and 10 days after finding by TAT, there was no change in 55% (76/139) of positive cattle.

• 대한수의학회지
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• 제40권1호
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• pp.130-137
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• 2000

In order to diagnose canine heartworm infection by antigen capture ELISA, the crude somatic(S), partial somatic(below 45kDa) and excretory/secretory(E/S) antigen of adult heartworm were identified and the antigenicity was examined by silver stain, immunoblot and ELISA. Then, production of monoclonal antibody to specific antigen carried out in this experiment. The bands to S antigen and E/S antigen were recognized between 10 and 200kDa and common bands were recognized strongly 14, 18, 28, 43kDa by silver stain. By western blot analysis, fractions to S antigen were recognized 14, 16, 18, 20, 24, 28, 32, 43, 50, 55kDa, etc. and only a 14kDa to E/S antigen in positive sera which were positive in modified Knott's test and necropsy. In ELISA, the positive sera reacted to antigens(SA, $SA_{45}$, E/S) were significantly different from negative sera by Student's t-test(p<0.05). Four hybridoma cell lines(14, 16, 17, 32kDa) than produce specific monoclonal antibodies for these antigens were obtained by immunizing BALB/c mice with a partially purified somatic antigen (below 45kDa) preparation, by fusing spleen cells with SP2/O cell myeloma cells, and by screening cell culture supernatants for antibody. In these results, it was confirmed that partial somatic antigen(below 45kDa) or E/S antigen can be used for serologic diagnosis of heartworm infection and monoclonal antibody reacting with specific antigen(14kDa) can be used for antigen capture ELISA in prepatent period of canine heartworm infection.

• 대한수의학회지
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• 제43권3호
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• pp.463-469
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• 2003

Eight nursery to grower pigs exhibiting weight loss and sudden death were diagnosed as postweaning multisystemic wasting syndrome (PMWS) based on the results of gross findings, histopathology, immunohistochemistry, fluorescent antibody test, virus isolation, PCR, serology, and electron microscopy. Groosly, the pigs had a rough hair coats and were severely emaciated. And moot lymph nodes were pale and enlarged. Lungs were not fully collapsed and exhibited 10 to 40% pale red cranioventral consolidation. Histopathologically, typical lymphohistiocytic interstitial to bronchointerstitial pneumonia, chronic lymphadenitis, severe lymphoid depletion, and basophilic intracytoplasmic inclusions were noted in the most lymphoid tissues. Porcine circovirus panicles were observed in the inguinal lymph node of the pigs by electron microscopy. Porcine circovirus type 2 (PCV2) antigens or viral DNAs were detected in the lesions of all pigs using immunohistochemistry or PCR. Two PCV2 were isolated from a homogenate of pooled lung and lymph node in 2 of the 5 pigs. Additionally, antigens of porcine reproductive and respiratory syndrome virus (PRRSV) and Hemophilus (H.) parasuis were also detected by immunofluorescent antibody test. Serologically, 55% of randomly selected sows and fattening pigs was serum antibody positive to PCV2 by an indirect fluorescent antibody (IFA) test and approximately 18 % of animals in the herd were serologically pooitive by the ELISA kit for PRRSV. To our knowledge, this is the first report of PMWS co-infected with PCV-2, PRRS, and H. parasuis in Korea.

• 대한수의학회지
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• 제34권3호
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• pp.529-536
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• 1994

To establish more specific and simple diagnostic methods for detection of the antibodies and antigens of Aujeszky's disease virus(ADV), we designed indirect dot-immunoassay(IDI) and double sandwich dotimmunoassay(DSDI) using the solid phases of nitrocellolose paper and polystyrene plate. The diagnostic efficacy of these methods was investigated. As the sensitivity of IDI was tested by various virus concentration, the specimens with the virus titer above $10^{4.0}TCID_{50}/0.2ml$ showed positive reaction, but that below $10^{1.0}TCID_{50}/ml$ revealed negative. Tonsil emulsion at the virus titer of $10^{4.5}TCID_{50}/0.2ml$ showed the highest sensitivity as diluted by 1/100. In detection of ADV antigens from the various tissues of the rats and pigs infected with ADV, IDI using monoclonal antibody showed the higher specificity as compared with IDI using polyclonal antibody and virus isolation method. The efficacy of the DSDI for detection of ADV antibody was compared with other tests. The sensitivity of DSDI was higher than virus neutralization(VN) and agar gel immunodiffusion test(AGID). Meanwhile, specificity of DSDI was lower than AGID, but similar to IDEA. In comparison with VN test, DSDI showed 96.9% agreement to VN test that is the highest of three tests. In general, application of polyclonal antibody in both tests caused the higher sensitivity but the lower specificty.

• 한국잠사곤충학회지
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• 제15권2호
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• pp.1-7
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• 1973

잠란 난령별 추출액을 항원으로 토끼의 항혈청을 만들고 이들 항원과 항체와의 사이에 생기는 반응에 의하여 항원성을 비교하였다. 그 결과는 아래와 같다. 1) 잠란내 난령별 조성물질은 토끼의 이정맥에 주입하였을 경우에 대부분의 잠란항원 A.B.D.G.H.J는 항체를 형성하였으나 그 일부의 항원 C.E.F.I에서는 항체 형성이 식별되지 않았다. 2) 잠란 잠령별 잠란내 조성물질은 생종인 대조 A구, B구와 대조 B구와 AKT 흑종 48시간구 D, 흑확잠 103과 잠 104에 있어서 G.H와 J는 서로 공통항원을 가지고 있었으나 그 일부 즉 AKT의 E.F 잠 104의 20시간구 I에서는 특이적인 항원항체반응을 인정할 수 없었으며 타 항체, D.E.F와의 사이에서는 비특이적인 항원항체반응을 인정할 수 있었다. 3) 각 난령별 잠란내에는 공통항원성 물질이 존재하는 것으로 사려되며 각 난령에 따른 특수항원성을 보기 위해서 흡습법에 의한 시험을 한 결과 B.D.G.J의 4개의 특이적인 항원을 인정할 수 있었다.

• Clinical and Experimental Reproductive Medicine
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• 제12권1호
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• pp.41-46
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• 1985

Various immunoserologic and cellular immunity techniques have been used to explore the presence of antisperm antibodies in the serum and seminal plasma of male patients and in the blood and genital fluid of infertile women. Several recent comparative investigations using various assays to detect and quantitate levels of antibody to human spermatozoa have produced widely varying results. So the first WHO workshop on iso- and autonatibodies to human spermatozoa in 1974 tried to establish some unification in the techniques used. The purpose of this study is to compare the results of two methods-the Kibrick macro-agglutination test and the Isojima micro-immobilization test-using the same test materials based on recommandation from WHO workshop. The results are as follows: 1. Twenty normal controls showed negative reactions in all the 2 tests. Out of 25 patients, the positive sera were noted in 15 (60%) on the Kibrick test and 13 (51%) on the Isojima test. 2. Twelve (48%) out of 25 patients showed positive reactions in the two tests, and 16 (64%) out of 25 patients showed positive reaction in one or more tests. 3. The titers of the antisperm antibodies on the Kibrick test was higher than that on the Isojima test. Therefore, it seems to be possible to increase the chances of detection of the antisperm antibodies, if two tests are imployed.

• 대한수의학회지
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• 제12권1호
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• pp.91-95
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• 1972

Investigation of leptospiral antibody in Korean cattle and pigs was carried out from February to October, 1971. Ten different living antigens, namely L. icterohaemorrhagiae, L. canicola, L. antumnalis, L. hebdomadis, L. australis A, L. pomona, L. pyrogenes, L. grippotyphosa, L. bataviae and L. javanica, were used. A total of 590 Korean cattle and 460 pig blood samples collected from Seoul Majang-dong slaughterhouse were tested by the rapid microscopic agglutination test. Throughout the studies the following results were obtained and summarized. 1. Of 590 serum samples of Korean cattle 51 were positive(8.64%). 2. Of 460 serum samples of pigs 27 were positive (5.87%). 3. Of 51 positive cattle samples, 29(4.92%) showed antibody to a serotype of L. icterohaemorrhagiae and 18(3.0%) to L. canicola, and 4 (0.68%) to L. pomona. Eight of L. icterohaemorrhagiae positive samples showed a cross reaction to L. canicola. 4. Of 27 positive pig samples, 14(3.04%) showed antibody to L. ictereohaemorrhagiae and 7(1.52%) to L. grippotyphosa. 4(0.87%) to L. canicola, 2(0.43%) to L. pomona. Two of L. canicola positive samples showed a cross reaction to L. grippotyphosa. 5. Serum samples of seven pigs, showing antibody positive to L. grippotyphosa were first observed in Korea. 6. Infection rate of bovine and porcine leptospirosis, in Korea, appeared to be lower than that of Japan, Taiwan, Thailand and the Philippines.

• 대한수의학회지
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• 제42권4호
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• pp.469-473
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• 2002

This study was carried out to investigate the blood groups(Aa, Ca, Dc, Qa, Ua) and antierythrocyte antibody associated with neonatal isoerythrolysis(NI) in Thoroughbred horses. The blood groups from 1,351(35 stallions, 1,316 mares) Thoroughbred horses tested by serological procedures, and antierythrocyte antibody from 52 mares by indirect antiglobulin test. The blood groups(factor) of Aa, Ca, Dc, Qa and Ua positive were 97.1%, 100%, 91.4%, 82.9%, and 17.1% in stallions, respectively, and were negative 3.5%, 6.2%, 25.1%, 18.3%, and 77.1% in mares, respectively. These mares are considered to be at risk for production of an NI foals. The antierythrocyte antibody was not detected by this technique in all mares. These results suggest that the all breeding mares without blood groups Aa(3.5%) and Qa(18.3%) should be selected a appropriate stallion to prevent of neonatal isretythrolysis during the breeding season in Thoroughbred horses.

• 대한미생물학회지
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• 제19권1호
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• pp.79-83
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• 1984

To determine the existence of anti-HLA antibodies finally in 220 human placental extracts to be proved negative antiserum by previous anti-HLA A,B,C antibody screening procedure, the present study was performed by fractionation of immunoglobulins using saturated ammonium sulfate and by simple batch method on DEAE cellulose. Thereafter using known 150 T-lymphocyte panels, complement-dependent microlymphocytotoxicity test was performed to observe the existence of anti-HLA antibodies and the degree of the antibody response of the concentrates. The following results were obtained: 1. Of total 141 placental sera concentrated 45 cases(31.9%) were showed significant anti-HLA A,B,C antibody response after concentration(Excellent, 19(13.5%), Good, 3(2.1%), Weak, 23(16.3%)). 2. Anti-HLA specificities of placental sera obtained after concentration were A2, A24, B13, B27, B44, B51, CN1, C7. 3. A new type C new-1 anti-HLA antibody that is only expressed in Korean people, was obtained. 4. 79 placental sera purrified by simple batch method using DEAE cellulose were showed negative anti-HLA antibody responses.

• 미생물학회지
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• 제32권4호
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• pp.280-284
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• 1994

공생 아메바에서 리소솜과 공생낭 간에 융합이 저해되는 이유로서는 먼저 이들 공생낭의 막에 어떤 특별한 인자가 존재하여 융합을 저해하거나 또는 융합 과정에 필수적인 어떤 요소가 이들 공생막에는 부족하여 융합이 일어나지 않는다고 유추해 볼 수 있다. 단일 클론 항체를 추적물질로 사용하여 이들 인자나 구성요소를 알아내는 과정에서, lipopolysaccharides가 공생 박테리아에 의하여 생산되어 공생낭의 막에 삽입된다는 것을 확인하였으며 이들이 공생막상에서도 세포질 방향으로 노출되어 있다는 것을 알아내었다. 따라서 이들 lipopolysaccharides가 리소솜과 공생낭간의 융합 저해에 간여하는 가를 알아보기 위하여 이들에 대한 단일 크론 항체를 공생 아메자의 세포질에 미세주사하여 보았다. 주사된 아메바에서는 공생낭과 리소솜간의 융합이 일어나는 것으로 미루어 보아, 아마도 lipopolysaccharides는 융합저해 요소 중의 하나로 사료되어 진다.