• Title/Summary/Keyword: Antibacterial test

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A study on the antibacterial activity and deodorization of fabrics dyed with pine needles extract (솔잎 추출물을 이용한 염색포의 항균성 및 소취)

  • Park, Young-Hee
    • Journal of Fashion Business
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    • v.14 no.1
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    • pp.176-183
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    • 2010
  • The purpose of this study is to examine the antibacterial activity and deodorization of fabrics dyed with pine needles extract. The microbes used for a test were two kinds of bacteria and two kinds of fungi. The test results showed as follows. In the test results of antimicrobial activity, both cotton and silk fabrics showed the antimicrobial rate of over 90% after washing three times. In the test result of antifungal activity, all fabrics didn't show any effective antifungal activity. In the test result of deodorization, the dyed cotton fabric showed the higher rate of deodorization than the non-dyed fabric, and the fabric showed the highest rate of deodorization was the test fabric mordanted by Fe and the rate of deodorization showed 92%. In case of silk fabric, the fabric showed the highest rate of deodorization was the test fabric mordanted by Al, and the rate of deodorization showed 92%.

Comparison of Antibacterial Ability of Air Filter Media Treated with a Natural Antibacterial Agent by Three Treatment Methods (항균성 천연물질의 도포방법에 따른 에어필터 여재의 항균 특성 비교)

  • Park, Sun Young;Jung, Jae Hee;Hwang, Gi Byung;Bae, Gwi-Nam;Kim, Yong Pyo;Nho, Chu Won
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.2
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    • pp.125-134
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    • 2013
  • Various antimicrobial technologies have been developed to inactivate bioaerosols in indoor environments. In this study, air filter media were treated with a natural antibacterial agent of Sophora flavescens in order to inactivate the bacteria collected on the air filter. Three treatment methods were applied to manufacture the test antibacterial air filter media: electrospray, nebulization and dip-coating methods. In case of electrospray and nebulization processes, the size distribution of aerosolized natural antibacterial agent was measured using a scanning mobility particle sizer. Staphylococcus epidermidis bacteria were aerosolized to test inactivation of the filter media treated with a natural antibacterial agent. The pressure drop and the antibacterial efficiency of the filter media increased with increasing the mass loading of natural antibacterial agent in each treatment method. The antibacterial efficiency per loaded antibacterial agent mass by the electrospray method was the highest and the filter treated by the dip-coating method was the lowest among three treatment methods.

A Study on the Antibacterial Activity of Chitosan

  • Chli, In-Ryu;Park, Jeong-Im
    • Proceedings of the Costume Culture Conference
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    • 2003.02a
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    • pp.24-25
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    • 2003
  • Water-soluble chitosan and water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% degree of deacetylation were controlled to evaluate the antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. The Shake Flask Method and Modified Shake Flask Method were used to find out the antibacterial activities of 5types of chitosan/acetic acid solution, and the other antibacterial activities test with the cotton filter treated with chitosan /acetic acid solution. Those test methods showed the great differences ,but the results of the antibacterial activites showed the same difference.

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Fabrication of Antibacterial Biodegradable films Using a Radiation-induced Reduction Method

  • Jung, Chan-Hee;Cho, Yong-Jun;Jung, Jin-Mook;Hwang, In-Tae
    • Journal of Radiation Industry
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    • v.7 no.2_3
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    • pp.141-147
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    • 2013
  • The simple and facile radiation technique of the preparation of antibacterial biodegradable polymer films containing silver nanoparticles (Ag NPs) was described. The biodegradable poly(butylene adipate-co-terephthalate) (PBAT) films containing silver trifluoroacetate (Ag TFA) were prepared by a solvent casting method, and then the films were irradiated by electron beams at the various doses ranging from 20 to 200 kGy to form Ag NPs in the biodegradable polymers. The results of UV-vis and FE-SEM/EDX analyses revealed that the Ag NPs were successfully formed in the PBAT matrix during the electron beam irradiation, and their amounts were dependant on the absorbed dose and Ag TFA concentrations. Furthermore, on the basis of the results of the antibacterial test through disk diffusion and colony counting test, the irradiated PBAT/Ag TFA films exhibited the antibacterial property due to the formation of Ag NPs.

Antibacterial Activity and Synergism of Hydnocarpi Semen Extracts with Ampicillin or Oxacillin against Methicillin-resistant Staphylococcus aureus

  • Joung, Dae Ki;Shin, Dong Young;Kwon, Dong Yeul;Shin, Dong Won
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.699-703
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    • 2016
  • Methicillin-resistant Staphylococcus aureus (MRSA) is a serious clinical and an urgent problem worldwide. Few new drugs are available against MRSA, because MRSA has the ability to acquire resistance to most antibiotics, which consequently increases the cost of medication. In the present study, the antibacterial activity of Hydnocarpi Semen was investigated. The most effective method is to develop antibiotics from the natural products without having any toxic or side effects. Therefore, there is a need to develop alternative antibacterial drugs for the treatment of infectious diseases. Five Clinical isolates (MRSA) were obtained from five different patients at Wonkwang University Hospital (Iksan, South Korea). The Other 2 strains were ATCC 33591 (Methicillin-resistant strain) and ATCC 25923 (Methicillin-susceptible strain). Antibacterial activity (Minimal Inhibitory Concentrations, MICs) was determined by broth dilution method, disk diffusion method, MTT test, and checkerboard dilution test. Antibacterial activity of n-hexane fraction was remarkable, and had a MICs ranging from $31.25-125{\mu}g/m{\ell}$. FICI values for HFH+AM and HFH+OX were 0.13-0.19 and 0.04-0.29, showing the increase of synergistic effect. When combined together, these antibacterial effects were dramatically increased.

A Study on Antibacterial Activity of Natural Material Treated Cotton Fabric (천연물 가공 면포의 항균성 연구)

  • 최인려
    • The Research Journal of the Costume Culture
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    • v.11 no.3
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    • pp.441-446
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    • 2003
  • Water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were used to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. As experimental method, Agar plate Smear Method and Agar plate Contact Method were used. The moleculur weight of chitosan didn't exert significant influences on its antibacterial activity against MRSA but chitosan having molecular weight 40,000, 80,000 and 150,000 showed the excellent antibacterial activity. The antibacterial efHciency was excellent in applying it after chitosan was dissolved in acetic acid solution, while the antibacterial efficiency was not expressed nearly in case of applying after chitosan was dissolved in neutral water. Therefore, it is considered that chitosan can show the antibacterial efficiency only if a positive ion status of -NH₃/sup +/ is maintained. MIC of chitosan/acetic acid solution and cotton fabrics finished with chitosan/acetic acid solution showed in concentration of 0.05%.

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Study on Antibacterial and Anti-Fogging using mugwort extract (쑥 추출물을 이용한 향균 및 김서림 방지에 관한 연구)

  • Bae, Sang-Dae
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.4
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    • pp.599-604
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    • 2020
  • Since Corona 19, awareness about antibacterial bacteria has changed. Hand washing after going out became a necessity. Also, fogging occurs a lot on the glasses depending on the wearing of the mask. This problem has caused a lot of discomfort in our daily life and changed our perception of antibacterial and anti-fogging. Therefore, in this study, we studied whether mugwort extract is effective in preventing antibacterial and fogging. To obtain the mugwort extract, mugwort was dried, alcohol extracted, and freeze-dried. An antibacterial test was performed using a mixture of mugwort extract, zinc oxide and natural zeolite mixed in 3:7, and a fogging test was performed using mugwort extract and other mixtures. As a result, in the case of E. coli and K. pneumoniae, which were used as test strains in the antibacterial test, the bacteria were not cultured from the medium added with 1% mugwort extract, and the antibacterial activity against representative Gram-negative bacteria was confirmed. In the anti-fog test, a 10 wt% mixture of mugwort extract showed very good results in both the hot and cold parts. We expect the mugwort extract to be effective in antibacterial and anti-fogging, thus helping to overcome Corona 19.

Antibacterial Activity of JiYu-san Against Methicillin-resistant Staphylococcus aureus (Methicillin resistant staphylococcus aureus에 대한 지유산의 항균활성)

  • Hwang, Hae;Kang, Ok-Hwa;Kwon, Dong-Yeul
    • Korean Journal of Pharmacognosy
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    • v.53 no.2
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    • pp.87-95
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    • 2022
  • Methicillin resistance Staphylococcus aureus (MRSA) is a gram-positive bacterium, the most commonly isolated bacterial human pathogen. JiYu-san is one of the natural products used to treat diseases in the folk recipe. In this study, we investigated the antimicrobial activity of EtOH 70% extracts of JiYu-san (JYS) against MRSA. The antibacterial activity of JYS against MRSA strain was evaluated using minimum inhibitory concentration (MIC), checkerboard dilution test, and time-kill assay. The effect of JYS on the immune mechanism of MRSA was confirmed through cell membrane permeability tests and energy metabolism tests, and the antibacterial activity mechanism was performed using qRT-PCR and western blot. As a result, in the antibacterial test of JYS, the MIC was measured to be 1.9~1000 ㎍/mL, and synergistic or showed a partial synergistic effect. In addition, JYS showed antibacterial activity in a combination test with DCCD or TX-100. In a study on the mechanism of action of antibacterial activity, it was found that JYS suppressed MRSA resistance genes and proteins. These results suggest that JYS has antibacterial activity and provides great potential as a natural antibiotic by modulating the immune mechanism against MRSA.

Antibacterial effect of tea tree on Streptococcus mutans (Streptococcus mutans에 대한 티트리의 항균 효과)

  • Choi, Yu-Ri;Kang, Min-Kyung
    • Journal of Korean society of Dental Hygiene
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    • v.17 no.4
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    • pp.613-620
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    • 2017
  • Objectives: The purpose of this study is to investigate the antibacterial effect of Streptococcus mutans of tea tree ingredient. Methods: The experimental groups were each given with different concentrations (30 or 50 vol%) of tea tree prepared in saline solution. The control group applied only saline solution. The tea tree coating of the specimen were examined under a scanning electron microscope. For the antibacterial activity test of the tea tree, the contact angle of the tea tree- coated specimen's surface was analyzed. The antibacterial effect against Streptococcus mutans was determined by counting the colony forming units (CFU). The statical statics were evaluated by using one-way ANOVA and paired t-test. Results: The tea tree treated group of hydrophilic more than non treated group. Antibacterial experiments demonstrated that tee tree solution was effective against Streptococcus mutans. However there was no significant difference in depending solution concentration groups. Conclusions: The antimicrobial activity of the tea tree containing solution showed its potential for use as coating for denture and medical materials.

Antibacterial Characteristics of PVA/PAA Hydrogel Film using Cefotaxime (Cefotaxime을 이용한 PVA/PAA 하이드로 겔 필름의 항균 특성)

  • Yeom, SeokJae;Jung, Sundo;Oh, Eunha
    • Journal of Environmental Health Sciences
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    • v.47 no.1
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    • pp.55-63
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    • 2021
  • Objectives: Cefotaxime is an antibiotic used to treat several bacterial infections. Specifically, it is used to treat pelvic inflammatory disease, meningitis, pneumonia, urinary tract infections, and sepsis. It is given by injection into either a vein or muscle. Antibacterial polymers prepared by chemical bonding and simple blending of antibacterials into polymers has attracted much interest because of their long-lasting antibacterial activity. This study attempted to review the possibility of hydrogel films as functional antibacterial materials by antimicrobial activity. Methods: In this study, CTX-PAA was synthesized by the chemical reaction of polyacrylic acid with cefotaxime by N,N'-Dicyclohexylcarbodiimide (DCC) method. Synthetic antibacterial hydrogel films were then prepared with PVA and CTX-PAA for functional application. Results: The increase in the cefotaxime content of the hydrogel films showed a similar decrease in tensile strength and elongation. The values of films impregnated with chemically bonded cefotaxime showed no significant difference. Antibacterial susceptibility was determined against Streptococcus pneumoniae and Escherichia coli using a standardized disc test. Conclusion: The synthetic antibacterial hydrogel films exhibited broad susceptibility against Streptococcus pneumoniae and Escherichia coli. Notably, the antibacterial effect of antibacterial hydrogel films against Grampositive (Streptococcus pneumoniae) was superior to that against Gram-negative (Escherichia coli).