• BMB Reports
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• v.29 no.6
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• pp.545-548
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• 1996

CA(1-8)ME(1-12), the CA-ME hybrid peptide of the amino terminal segments of cecropin A (CA) and melittin (ME), has been reported to have a broad spectrum and improved potency without a hemolytic property. In order to obtain new synthetic peptides with powerful antibacterial activity without hemolytic activity, several hybrid peptides were designed from the sequences of CA, ME, magainin 2, bombinin and lactoferricin. All hybrid peptides were constructed to form an amphipathically basic-flexible-hydrophobic structure and synthesized by the solid phase method. Their hemolytic activities against human red blood cells and antibacterial activities against both Gram-positive and Gram-negative bacteria were detennined. CA(1-8)MA(1-12), CA(1-8)BO(1-12), MA(10-17)ME(1-12) and LF(20-29)ME(1-12) showed comparable activities with broad spectra against both Gram-positive and Gram-negative bacteria relative to CA(1-8)ME(1-12) but without hemolytic properties. These hybrid peptides, therefore, could be useful as model peptides to design a novel peptide with improved antibacterial activity and study on structure-activity relationships of antimicrobial peptides.

• Korean Journal of Medicinal Crop Science
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• v.24 no.2
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• pp.129-135
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• 2016

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

• The Korea Journal of Herbology
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• v.20 no.4
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• pp.41-51
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• 2005

Objectives : The purpose of this research was physiological activities, cytotoxicity, and antibacterial activities of the Cheongyeolsodokum and its constituent herbs. Methods : Physiological activities, cytotoxicity, and antibacterial activities were examined through the Cheongyeolsodokum and its constituent herbs. Results : In the physiological activities, the electron donating ability(EDA) of the water and ethanol extracts from the Cheongyeolsodokum were over 60% and 80% at 100 ppm, respectively. The EDA of the water and ethanol extract from the Cheongyeolsodokum ingredients were gradually increased as well. Water and ethanol extracts from the Cheongyeolsodokum and its constituent herbs inhibited xanthine oxidase activity, they showed superoxide dismutase(SOD)-like activity. The Cheongyeolsodokum and its constituent herbs inhibited cancer cell growth in a dose-dependant manner. Also, the clear zones against Staphylococcus aureus and S. epidermidis were clear shown at 2.5 and 5 mg/disc. Its constituent herbs showed the clear zone against various bacteria such as Candida albicans, S. aureus, S. epidermidis, and S. mutans were shown at 0.5 and 1mg/disc. Conclusions : We observed physiological activities, cytotoxicity, and antibacterial activities of the Cheongyeolsodokum and its constituent herbs. The results also indicated that water extract and ethanol extract of Cheongyeolsodokum and its constituent herbs can be used as a natural ingredient in food or cosmetic industry.

• Carbon letters
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• v.4 no.3
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• pp.140-145
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• 2003

Antibacterial behaviors of PAN-based activated carbon fibers (ACFs) containing silver metal were investigated. The effects of surface and pore structures of the ACFs were studied by $N_2$/77 K adsorption and D-R plot as a function of silver loading content. The antibacterial activities were investigated by a dilution test against Staphylococcus aureus (S. aureus; gram positive) and Klebsiella pnemoniae (K. pnumoniae; gram negative). As experimental results, the ACFs showed some decreases in specific surface areas, micropore volumes, and total pore volume with an increase of silver content. However, the antibacterial activities of the ACFs were strongly increased against S. aureus as well as K. pnumoniae, which could be attributed to the presence of antibacterial metal in the ACFs system.

• ALGAE
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• v.36 no.1
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• pp.61-72
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• 2021

Increased proliferation of bacterial resistance to antibiotics is a critical issue that has increased the demand for novel antibacterial compounds. Antibacterial activities have been evaluated in extracts from photosynthetic green microalgae, with varying levels of subsequent potential for development based on the strain of algae, strain of bacterial pathogen, and solvent used to extract the metabolites. Green microalgae from extreme environmental conditions have had to adapt to conditions that exclude many other organisms. The production of antibacterial compounds aids directly or indirectly in the survival of green microalgae in these extreme environments, as well as potentially serve other roles. This review investigates antibacterial activities of green microalgae from both extreme in-situ environmental conditions and induced extreme laboratory conditions and highlights.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.345-352
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• 2023

Antibacterial activity against foodborne bacteria (Bacillus cereus, Staphylococcus aureus, Salmonella Enteritidis) and inhibitory activity against murine norovirus, a human norovirus surrogate, of 28 extracts from plants endemic to Korea were investigated in this study. All plant extracts showed antibacterial activity only against gram-positive bacteria, B. cereus and S. aureus. Extracts from Callistemon speciosus and Nymphaea tetragona showed inhibition zones of 16.54 and 24.35 mm against B. cereus and S. aureus, respectively, presenting the highest antibacterial activities recorded in this study. Among all samples, Ardisia japonica extract at concentrations of 100 and 200 ㎍/mL showed the highest virucidal activities of 96.6 and 100.0%, respectively. Ardisia japonica, Duchesnea indica, Polygonum aviculare, and Geum japonicum extracts showed high antibacterial and virucidal activity simultaneously without Raw 264.7 cell cytotoxicity. These plant extracts may serve as potential antimicrobials to control foodborne infections.

• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.49-55
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• 2006

Antioxidative and antibacterial activities of endemic plants extracts in Korea were investigated. Hydrogen radical scavenging activity was 99.72% in an elm tree, 99.725 in a Job's tears, 99.575 in an eggplant, and 94.025 in a barrenwort. Antibacterial activity of ethanol extract from 23 different species of wild plants were determined. The gull nut was showed the strongest antibacterial activities (16.0-19.0 mm) and also showed high antibacterial activities in a pine neddles, a gulmyungja, a wild ginger plants, a ginko (9.5-11.5 mm). Only extracts of Saurrranceae extract showed the antibacterial activity in Bacillus subtilus, Stapylococcus aureus, Escherichia coli, Samonella entetotidis. An antibacterial activities were showed weakly in an esungcho and an eggplant. A ground ivy showed Antibacterial activity on Stapylococcus aureus. A barren wort showed in antibacterial activity on Stapylococcus aureus, and E. coli. This results were suggested that many edemic plants resources contains antioxidative and antibacterial substances.

• Korean journal of food and cookery science
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• v.26 no.2
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• pp.206-213
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• 2010

In this study, the antibacterial activities of essential oil from Zanthoxylum schinifolium against four Gram-positive bacteria and six Gram-negative bacteria were investigated. The antibacterial activity of the oils was determined using the agar-well diffusion assay, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration). In particular, essential oil from Z. schinifolium showed higher antibacterial activity against Gram-positive bacteria than against Gram-negative bacteria. Essential oil from Z. schinifolium displayed large inhibition zones especially against Bacillus cereus (31 mm). At concentrations between 0 and $20\;{\mu}g/mL$ the oils showed an antibacterial effect against both Gram-negative and Gram-positive bacteria. The minimum inhibitory concentration (MIC) values against nine bacteria ranged from 1.25 to $5\;{\mu}g/mL$. The minimum bactericidal concentration (MBC) values against eight bacterial ranged from 2.5 to $20\;{\mu}g/mL$, except Shigella sonnei. Furthermore, our finding on the antibacterial activities of essential oils from Zanthoxylum schinifolium validated the use of this plant for medical purposes.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.246-253
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• 2016

The antibacterial activities of thirty mushroom species belonging to Basidiomycetes and Ascomycetes, cultivated on two liquid media, were evaluated against gram-positive (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria by the disk diffusion method. All of the mushrooms, except Auriporia aurea, Fomes fomentarius, and Lyophyllum shimeji, showed different antibacterial activity levels—from 9.5 mm in diameter of the inhibition zone to full inhibition of growth of the test bacteria. The antibacterial activities of Crinipellis schevczenkovi, Hohenbuehelia myxotricha, Oxyporus obducens, and Spongipellis litschaueri were observed for the first time. The antibacterial potential of culture liquids of the investigated species was higher than that of their mycelia activity. Dependence of the intensity of antibacterial activity on the culture medium was shown. The antibacterial efficiency of the most active species (Lentinus edodes, Piptoporus betulinus, and Phellinus igniarius) was verified and compared with those of some commercial antibiotics and natural essential oils of Salvia and Eucalyptus. The culture liquid of Piptoporus betulinus, obtained after cultivation on glucose-peptone-yeast culture medium, is a potential substance for further creation of antibacterial products.

• Archives of Pharmacal Research
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• v.14 no.3
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• pp.195-198
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• 1991

As potential antibacterial agents, several antipyrine derivatives were synthesized from 4-formylantipyrine and antibacterial activities of the synthesized compounds were also examined.