• Food Science and Biotechnology
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• 제17권3호
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• pp.547-552
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• 2008

The detection of carbamate (carbofuran, carbaryl, benfracarb, thiodicarb, and methomil) and organophosphate (diazinon, cadusafos, ethoprofos, parathion-methyl, and chlorpyrifos) pesticide residues with very low detection limits was carried out using surface plasmon resonance (SPR) based equipment. The capacity to develop a portable SPR biosensor for food safety was also investigated. The applied ligand for the immunoassays was polyclonal goat anti-rabbit immunoglobulin (IgG) peroxidase conjugate. Concentration tests using direct binding assays showed the possibility of quantitative analysis. For ligand fishing to find a proper antibody to respond to each pesticide, acetylcholinesterase (AChE), and glutathione-S-transferase (GST) were tested. The reproducibility and precision of SPR measurements were evaluated. With this approach, the limit of detection for pesticide residues was 1 ng/mL and analysis took less than 11 min. Thus, it was demonstrated that detecting multi-class pesticide residues using SPR and IgG antibodies provides enough sensitivity and speed for use in portable SPR biosensors.

• Applied Science and Convergence Technology
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• 제25권3호
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• pp.61-65
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• 2016

Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.

• Transactions on Electrical and Electronic Materials
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• 제7권4호
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• pp.196-203
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• 2006

An air-gap type FBAR was designed using Leach equivalent model for analyzing a vertical structure of the FBAR. For the top electrode, Pt, and the bottom electrode, Au, of $1.2{\mu}m$ thickness and the piezoelectric of 0.8,urn thickness, the resonance and anti-resonance occurred at 2.401 GHz and 2.460 GHz, respectively. $S_{11}$ was increased and $S_{21}$ was decreased as the resonance area of FBAR was widened. We observed the characteristics of insertion loss, bandwidth and out-of-band rejection of ladder-type FBAR BPF by changing resonance areas of series and shunt resonators and by adding stages. As the resonance area of series resonator was increased, insertion loss was improved but out-of-band rejection was degraded. And as the resonance area of shunt resonator was increased, insertion loss was degraded a little but out-of-band rejection was improved even without adding stages. We, also, changed the shape of the resonance area from square shape to rectangle shape to examine the effects of the resonator shape on the characteristics of the BPF. The best performances were observed when the sizes of series and shunt resonator are $150{\mu}m{\times}l50{\mu}m\;and\;5{\mu}m{\times}50{\mu}m$, respectively. Out-of-band rejection was improved about 10dB and bandwidth was broadened from 30MHz to 100MHz utilizing inductor tuning on $2{\times}2\;and\; 4{\times}2$ ladder-type BPFs.

• 대한의생명과학회지
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• 제25권3호
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• pp.227-236
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• 2019

In this study, the anti-oxidant, anti-inflammatory, and anti-obesity activities of an ethanol extracts of Ulmus divididiana var. japonica (UDE) were investigated in vitro and in vivo. UDE anti-oxidant activity was evaluated with an Electron Spin Resonance (ESR) spectrometer, which measured 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. Cell viabilities were estimated using 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. LPS-stimulated BV-2 microglia were used to study the production of nitric oxide (NO). Cells stimulated with LPS produce more NO than normal control cells. However, cells treated with the UDE decreased this production in a concentration dependent manner (100, 250, 500, $1,000{\mu}g/mL$). Also, we investigated the anti-obese activity of UDE in SD rats. The SD rats were randomly divided into five groups: 10% low fat diet (N), 45% high fat diet (H), 45% high fat diet + garcinia extracts 200 mg/kg/day (HG200), high fat diet + UDE 200 mg/kg/day (HU200), high fat diet + UDE 400 mg/kg/day (HU400). UDE was found to lower whole body and abdominal and epididymal adipose tissue weights and lowered plasma levels of triglyceride (TG), compared to those in H group. Histological analyses of the liver and fat tissues of rat treated with UDE revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the H group. These results suggest that UDE might be used to develop potent anti-oxidant, anti-inflammatory, and anti-obesity agents, and may be useful as ingredients for related new functional raw materials.

• 동아시아식생활학회지
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• 제22권6호
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• pp.772-781
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• 2012

This study was conducted to examine the development of a new anti-inflammatory substance with potent anti-inflammatory activities that was derived from the Platycodi Radix butanol fraction. To accomplish this, the chemical structures and anti-inflammatory activities of the components were elucidated. Upon column chromatography of the tertiary subfraction, fractions 8-4-1 and 8-4-2 were identified as platycodin D and D3, respectively, following recrystallization, based on melting point (MP), infrared (IR), and positive fast atom bombardment (FAB)-mass and nuclear magnetic resonance (NMR) spectral data. Platycodin D and D3 exhibited strong anti-inflammatory activities in rats when administerd at oral doses of 12 mg/kg and 36 mg/kg, p.o., respectively. Platycodin D and D3 induced inhibitory effects on capillary vascular permeability in rats at oral doses of 16 mg/kg and 24 mg/kg, p.o., respectively, and potent inhibition of leukocyte emigration in a carboxymethyl cellulose (CMC)-pouch when administered at doses of 3 mg/rat and 7 mg/rat, s.c., respectively. These results verified the high antiinflammatory potency of the platycodin D and D3 components in Platycodi Radix.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2007년도 학술대회
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• pp.46-46
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• 2007

• Macromolecular Research
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• 제16권2호
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• pp.182-184
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• 2008

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 추계학술대회
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• pp.78-81
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• 1995

In imaging the samples or human internal organs in a tube shape, general RF-probe types (that encircles a sample or places on top of the sample) are usually unsuitable for the internal imaging due to the degradation of signal-to-noise ratios(SNR's). In the present study the inside-out probes for Magnetic Resonance Imaging (MRI) have been constructed in the three different shapes such as an anti-solenoidal, a saddle and a dual surface types which are positioned as close to the area as possible by putting the probe inside the tubelike sample to improve filling factor. RF-field distributions have also been calculated depending upon the geometrical changes of anti-solenoid probes. Moreover, the performance of the inside-out probes has been checked by measuring SNR's of the images acquired. The inside-out probes constructed in this study produced better SNR's and rf-field uniformity in the area close to the probes in comparing with any other commercial probes. There is a high possibility that the constructed probes in the present study are applicable to the diagnosis of human bodies.

• Journal of electromagnetic engineering and science
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• 제18권4호
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• pp.267-269
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• 2018

In this letter, the null steering of a circular array is presented using a modified array factor (AF) for a global positioning system (GPS) anti-jam. The seven radiating elements were designed using a mu-zero resonance (MZR) circularly polarized (CP) antenna arranged toward the center. Since the radiating elements, which are arranged toward the center, have a CP characteristic, the AF of the seven radiating elements has to be modified considering the rotation angle of the nth radiating element. The phases of input ports can be calculated to implement a nulling of radiation patterns where the modified AF is zero. To verify the modified AF for null steering in the desired direction, two cases of power dividers operating in $L_2$ band (1.2276 GHz) were fabricated to achieve pattern nulling at a certain angle. The modified AF can be confirmed by a comparing the simulated and measured radiation patterns.

• 조명전기설비학회논문지
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• 제29권6호
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• pp.96-108
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• 2015

his study deals with a power conversion unit of an anti-start air conditioner compressor system for the commercial vehicle. In case of converter, to reduce the switching losses and current stresses of the device, applies LLC resonant topology. Transformer leakage inductance in the full-bridge converter is used for making the resonance with the capacitor of the voltage-doubler. Through this method we can increase power and decrease volume of system. Both circuit analysis and design guideline are described. So in this paper, power conversion unit is designed. 2.5kW anti-start air conditioner compressor system was implemented, and system operation and stability was verified through experiment.