• Industry Promotion Research
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• v.9 no.4
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• pp.143-151
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• 2024

In this experiment, we evaluated the anti-microbial, anti-oxidant and anti-platelet activities using 70% ethanol extract of natual products, Natural products (Lycium chinense, Aralia cordata, Achyranthes japonica, Mentha canadensis, Eriobotrya japonica, Belamcanda chinensis, Phlomis umbrosa, Lespedeza cuneata, Kalopanax septemlobus, Nelumbo nucifera) were extracted with 70% ethanol, freeze-dried, and used in the experiment. All natural products exhibited antibacterial activities, and Aralia cordata, Achyranthes japonica, Eriobotrya japonica, Belamcanda chinensis, and Nelumbo nucifera showed strong antimicrobial effect. Next, we investigated the antioxidant activity, polyphenol and flavonoid content and antiplatelet activity of the natural products. As a result, Aralia cordata, Achyranthes japonica, Eriobotrya japonica, Belamcanda chinensis, and Nelumbo nucifera showed antiplatelet effect and Ca²⁺ inhibitory activity. Therefore, it was confirmed that the five natural product extracts have a potential to prevent antibacterial, antioxidant, and antiplatelet-related diseases, and it is suggested that they can be developed as materials for functional foods and pharmaceuticals in the future.

• Journal of Applied Biological Chemistry
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• v.54 no.1
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• pp.51-55
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• 2011

To develop a health-aid preparation of Dioscorea batatas (DB), lactic acid fermentation was attempted using a mixed starter comprising of Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium longum. The anaerobic fermentation of a 10% DB flour suspension gave a uniform suspension of pH 3.65, containing $8{\times}10^6$ CFU/mL lactic acid bacteria. During the administration of the lactic acid fermented DB (FDB) and DB to trinitrobenzene sulfonic acid (TNBS)-induced colitis mouse model, histological lesions, morphological damage, and myeloperoxidase acitivity were significantly reduced at a dosage of 200 and 400 mg/kg/day. Dose-response (200 and 400 mg/kg/day) studies revealed that FDB pre-treatment of mice significantly ameliorated the appearance of diarrhoea and the disruption of colonic architecture. In FDB-pretreated mice, there was a significant reduction in the degree of both neutrophil infiltration (measured as decrease in myeloperoxidase activity) and weight loss rates. Theses findings suggest that FDB exerts beneficial effects in experimental colitis and may be useful in the treatment of inflammatory bowel disease.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.127-133
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• 2001

Pseudomonas aeruginosa KLP-2 possessing antimicro- bial activity against Legionella pneumophila was isolated from cooling tower-waters. The culture filtrates of P. aeruginosa KLP-2 showed antimicrobial activity against L. pneumophila Vibro cholerae non-Ol. Bacillus cereus, Bacillus subtilis and staphylococcus aureus. The culture filtrates of P. aeruginosa KLP-2 showed the highest anti-microbial activity against. L pneumophila among micoorganisms tested in this study. The optimal conditions of temperature, pH carbon source and nitogen source to obtain maximal antimicrobial activity from culture filtrates of P. aeruginosa KLP-2 were determined to be 35$^{\circ}C$ pH 7.0 % of glycerol and 0.6% of proteose peptone respec- tively. The antimicrobial activity of culture filtrates from P. seruginosa KLP-2 was highest against L. pneumophila when cultivated with shaking for 24 h and without shaking for 4 day at 35$^{\circ}C$.

• Korean Journal of Food Science and Technology
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• v.37 no.1
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• pp.84-89
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• 2005

Mongolian dandelion (Taraxacum mongolicum H.) extracted with solvents having different polarities were prepared to investigate protection activity of gastric mucosa. Ethanol extracts were successively reextracted with methylene chloride, and n-butanol. Concentrations of total flavonoids and luteolin in n-butanol extracts were 27.75 and 1.14%, respectively, much higher than those of other solvent extracts. Results of anti-microbial acitivity test against Helicobacter pylori and urease inhibition test revealed n-butanol extract exerted higher inhibition (13.16%) than other solvent extracts. Based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, n-butanol extract showed the highest efficiency of free radical-scavenger activity among the extracts ($SC_{50}\;47{\mu}g/mL$). Erythrocytic photohemolysis tests, for the protection of cell membrane showed that half-life of human erythrocytes was increased by the addition of n-butanol extract $({\tau}_{50}=172min;C=5.0{\mu}g/mL)$. These results indicate n-butanol extract of Mongolian dandelion may be useful as a adjuvant for gastric diseases.