• 대한한의학회지
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• 제39권4호
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• pp.86-113
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• 2018

Objectives: We examined the effects of a mixed formula consisting of dried pomegranate concentrate powder (PCP) and the aqueous extracts of Eucommiae cortex (EC) and Achyranthis radix (AR) in rats with surgically induced osteoarthritis (OA). Methods: Two weeks after OA-inducing surgery, a PCP:EC:AR 5:4:1 (g/g) combination or single formula was orally administered. Changes in body weight, knee thickness, maximum knee extension angle, bone mineral density of the knee joints, femoral and tibial articular surfaces, and compressive strength of the femoral and tibial articular cartilage (AC) were assessed, along with the prostaglandin E2 level, 5-lipoxygenase, matrix metalloproteinase (MMP)-2 and MMP-9 activity, and chondrogenic gene mRNA expression in the femoral and tibial AC with the synovial membrane (SM). In addition, the number of cleaved poly(ADP-ribose) polymerase, cyclooxygenase and tumor necrosis factor-${\alpha}$-immunoreactive cells in the femoral and tibial AC with SM were monitored, and the rate of cell proliferation was determined with a 5-bromo-2'-deoxyuridine uptake assay. Results : The signs of surgically induced OA in rats were significantly inhibited by both PCP, EC and AR combined and single formulas. In particular, the combination formula-treated OA model rats showed dose-dependent, significantly increased inhibitory activity against all tested criteria compared with single formula-treated rats. Conclusions: Taken together, our results suggest that the combination formula synergistically increased the anti-OA effects of its components through anti-inflammatory and chondrogenic activity in rats with surgically induced OA. In addition, 200, 100 and 50 mg/kg combination formula treatments showed dose-dependent inhibitory activity against all of the tested criteria.

• 대한한의학회지
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• 제38권4호
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• pp.11-40
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• 2017

Objectives: We investigated whether a mixture of the main component of pomegranate concentrated powder (PCP) with appropriate proportions of Eucommiae Cortex (EC) and Achyranthis Radix (AR) could act synergistically as an effective treatment for osteoarthritis (OA). Methods: In order to evaluate the effects of PCP, EC, and AR against OA, knee thicknesses, maximum extension angle of each knee, anti - inflammation effects, the transcript levels of chondrogenic genes mRNA expressions in femur and tibia articular cartilage (AC) with synovial membrane (SM) were analyzed. In addition, the histopathology and immunohistochemistry of the femur and tibia AC or SM were performed. Results: The surgically-induced OA signs in rats were significantly inhibited by 28 days of continuous treatment of PCP, EC and AR single formulas, and PCP with EC:AR mixed formulas. Especially, PCP with EC:AR 4:1, 2:1 and 1:1 mixed formula treatment constantly showed significantly more favorable inhibitory activities, as compared with those of single formula of PCP, EC and AR treated rats. Conclusion: PCP and EC:AR 4:1 mixed formula showed similar OA refinement effects through potent anti-inflammatory pathways as compared with those of diclofenac treatment, and showed additional chondrocyte proliferating effects on the both femur and tibia AC.

• 동의생리병리학회지
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• 제33권2호
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• pp.131-140
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• 2019

The objective of present study is to evaluate concentration-dependent in vitro anti-osteoarthritic (OA) effects of synergic mixed formula consisted of dried pomegranate juice concentrate powder, Eucommiae Cortex aqueous extract and Achyranthis Radix aqueous extract 5:4:1 (g/g) mixture on the primary cultured rat articular chondrocytes. First, any cytotoxic effect of mixture was observed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide) assay. Next, cyto-protective effect of test substances was evaluated by using the recombinant human interleukin $(rhIL)-1{\alpha}$ induced chondrocytes. In addition, anti-inflammatory effects were also observed on the lipopolysaccaride (LPS) treated chondrocytes through prostaglandin $E_2(PGE_2)$ productions and 5-lipoxygenase (LPO) activities, and inhibitory effects on matrix metalloproteinase (MMP)-2 and MMP-9 activities were observed on $rhIL-1{\alpha}$ treated chondrocytes with their extracellular matrix (ECM) related mRNA expressions. No obvious cytotoxic effects of mixture were demonstrated. Inflammatory damages of chondrocytes and related ECM degradations induced by treatment of LPS or $rhIL-1{\alpha}$ were significantly and concentration-dependently inhibited by pretreatment of mixture from a concentration level of 0.001 mg/ml to 1 mg/ml. In addition, mixture showed $IC_{50}$ for $rhIL-1{\alpha}-induced$ MMP-2 and MMP-9 activities as 44.01 and $162.47{\mu}g/ml$, and also showed $EC_{50}$ for $rhIL-1{\alpha}-induced$ inhibition of collagen type II, SOX9 and aggrecan mRNA expression as 8.61, 10.79 and $4.47{\mu}g/ml$, respectively. It is observed that mixture showed concentration-dependent anti-inflammatory and cytoprotective ECM preserved effects on the primary cultured rat articular chondrocytes without cytotoxicity.

• 통상정보연구
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• 제15권2호
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• pp.233-260
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• 2013

FTA를 중심으로 하는 다수의 RTA는 미래 세대에 대한 배려, 지속성, 공정성, 형평성 등을 핵심적인 가치로 하는 지속가능한 발전 개념을 내포하고 있는 경우가 점차 증가하고 있고, 자유무역주의 기조의 확산 정착과 지속가능한 발전 간의 조화를 위한 중요한 규범적 도구가 될 수 있을 것으로 기대되고 있다. 따라서 국제사회가 모색하고 있는 새로운 세계경제 질서의 재편 및 확립을 위한 세계경제의 통합과 자유화에 있어서는 그 어느 때보다도 지속가능한 발전개념이 핵심적인 규범 목적과 가치로 주목받고 있다. 반덤핑제도의 주요 초점이 과거에는 덤핑을 규제하기 위한 것이 목적이었으나, 최근에는 반덤핑제도의 남용을 억제하는 것으로 변화되고 있다. 반덤핑제도 운영의 투명성이 무엇보다도 중요하며, 객관적이고 투명한 반덤핑제도의 운영은 행정적 효율성을 제고시켜줄 뿐만 아니라 수출국에 대한 보복 조치와 무역 분쟁을 방지할 수 있기 때문이다. 따라서 국내적 대응방안으로 덤핑수출업체로부터의 국내산업 보호를 위한 정책을 수립하여야 하며, 비교열위산업의 경우 해당부문에 대한 구조조정을 촉진하여야 하고, 산업구조 고도화를 위한 정부의 정책조정이 필요할 것이다.

• 대한예방한의학회지
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• 제21권3호
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• pp.87-98
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• 2017

Objectives : The objective of present study is to evaluate anti-arthritic effects of dried pomegranate concentrate powders (PCP), Eucommiae Cortex aqueous (EC) and ethanolic (ECe) extracts, Achyranthis Radix aqueous (AR) and ethanolic (ARe) extracts on the primary cultured rat articular chondrocytes. Methods : MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide) assay was performed cytotoxic effect of test substances. In addition, anti-inflammatory effects were also observed on the lipopolysaccaride (LPS) treated chondrocytes through prostaglandin $E_2\;(PGE_2)$ production and 5-lipoxygenase (LPO) activities, and inhibitory effects on metalloproteinase (MMP)-2 and MMP-9 activities were observed on the recombinant human interleukin $(rhIL)-1{\alpha}$ treated chondrocytes with their extracellular matrix (ECM) related mRNA expressions - collagen type II, SOX9 and aggrecan. Results : As results, ECe and ARe showed obvious cytotoxicity against primary cultured rat articular chondrocytes at a dose level of 10 mg/ml, respectively. However, no obvious cytotoxic effects of PCP, EC and AR were demonstrated at a dose level of 10 mg/ml, on the primary cultured rat articular chondrocytes. In addition, treatment of LPS $50{\mu}g/ml$ induced significant increases of $PGE_2$ contents and 5-LPO activities indicating inflammatory responses of the primary cultured rat articular chondrocytes, and also decreases of cell viabilities, increases of MMP-2 and MMP-9 activities with decreases of extracellular matrix (ECM) related collagen type II, SOX9 and aggrecan mRNA expressions were observed by treatment of $rhIL-1{\alpha}$ 50 ng/ml, suggesting damages on the primary cultured rat articular chondrocytes and related ECM degradations. However, these inflammatory responses and related ECM degradations were inhibited by pretreatment of all test substances, in order of PCP > ECe > ARe > EC > AR, and $rhIL-1{\alpha}$ induced chondrocytes deaths are inhibited by treatment in order of PCP > EC > AR > ECe > ARe. Conclusions : Taken together, it is expected that mixed formulation of PCP as main components with appropriate proportion of EC and AR as additional components will be achieved a potent alternative medicinal food for osteoarthritis.

• Nutrition Research and Practice
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• 제12권6호
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• pp.486-493
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• 2018

BACKGROUND/OBJECTIVE: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and $300{\mu}g/mL$. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with $300{\mu}g/mL$ of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or $300{\mu}g/mL$ of HBC and HBK (P < 0.01). Treatment with $300{\mu}g/mL$ of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with $300{\mu}g/mL$ of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.

• International Journal of Computer Science & Network Security
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• 제22권1호
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• pp.69-76
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• 2022

At the current stage of globalization and European integration of Ukraine, the aspects related to the effective fight against corruption in the system of economic security of our country are receiving more and more attention, as they become a prerequisite for continuing reforms based on international funding. In order to consider this issue and solve this problem, the necessary step is to develop and implement real mechanisms of the system for detecting and preventing corrupt behavior, which are based on international anti-corruption standards. The leading component of this system is the management of corruption risks in the system of economic security in order to identify them and implement measures to reduce them. This study analyzes the corruption perception index in Ukraine in recent years, which showed a positive, albeit somewhat slow dynamics of its growth, indicating a gradual increase in overcoming corruption through the introduction of a number of anti-corruption measures and changes. It is proved that the current stage of socio-economic development of the country contributes to strengthening the processes of combating corruption and preventing corruption risks, creating an effective and efficient anti-corruption system of the state. The concept of "corruption" was studied, it was found that in the field of public administration it is considered from different positions and is closely related to the concept of "corruption risks". The essence and features of corruption risks are studied, the preconditions of their occurrence are formulated, the relationship between the causes of corruption risks and economic security in the field of public authority has been established. The system of corruption risk management is considered and its components are characterized. It is proposed to increase the effectiveness of anticorruption policy through the implementation of measures aimed at investigating the causes of corruption risks, as well as developed effective and effective means of reducing corruption risks within the system of economic security

• 한국약용작물학회지
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• 제18권4호
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• pp.217-223
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• 2010

In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with $RC_{50}$ value of $10.5{\pm}1.4{\mu}g/m{\ell}$, reducing power value 1.04 Abs (concentration of 1 mg/$m{\ell}$), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 mg/$m{\ell}$). M80 extract showed the high content of total phenolic (319.7 mg GAE/$m{\ell}$ extract) and total flavonoid (111.6 mg QE/$m{\ell}$ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging ($R^2$=0.911 and 0.912, each extract) and reducing power ($R^2$=0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity ($R^2$=0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.

• 한국사회복지학
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• 제57권1호
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• pp.271-297
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• 2005

본 연구는 룩셈부르크소득연구의 가구소득자료를 이용하여 1980년대 이래 서구 복지국가에서 빈곤의 실태는 어떻게 변화하고 있는가를 분석하고, 빈곤의 변화추세를 인구집단별 및 소득원별로 분해함으로써 경제사회구조 및 공적소득이전제도의 변화가 빈곤의 추세와 어떠한 관련을 맺고 있는지를 탐색하였다. 연구결과는 먼저 대부분의 서구 복지국가에서 빈곤이 심화되는 추세에 있음을 보여 준다. 이처럼 빈곤이 심화되는 추세에 있는 것은 노령층이나 편부모가구 등 전통적으로 빈곤의 위험이 높은 인구집단이 증가하고 있는 때문만은 아니다. 노령층의 경우 연금제도 등 공적소득이전의 영향으로 인해 생산연령층에 비해 빈곤상태가 낮은 수준을 보이고 있으며 시장소득의 빈곤도 점차적으로 감소하는 추세에 있다. 편부모가구는 전반적으로 생산연령층 일반가구에 비해 빈곤의 정도가 심한 편이나 빈곤의 변화추세는 노동시장정책 및 공적소득이전제도의 차이에 따라 국가별로 크게 다르게 나타난다. 서구 복지국가에서 빈곤이 심화되는 것은 무엇보다 생산연령층 인구의 빈곤, 특히 시장소득 빈곤이 심화되고 있는데서 그 원인을 찾을 수 있다. 경제의 세계화와 노동시장 유연화에 따른 실업 및 비정규직의 증가, 저임금 노동자의 증가 등 최근의 경제환경 변화가 생산연령층 인구의 빈곤을 증가시키는 요인으로 작용할 가능성이 높은데, 이에 대한 구체적이고 깊이 있는 분석과 함께 이 계층에 대한 빈곤정책이 확대될 필요가 있다.

• 동남아시아연구
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• 제27권2호
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• pp.1-36
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• 2017

본 연구는 개혁시기 들어 인도네시아의 오랜 자경주의 경관 속에 처음으로 등장한 이슬람적 치안의제와 그 실천 주체로서의 이슬람 자경단에 관한 연구이다. 이를 위해 본고는 이전 시기까지 인도네시아 사회가 일상공간에 관한 치안의제를 정의해 온 방식들과의 연관성 속에서, 이슬람적 치안 발생을 가능케 한 시대 배경, 이슬람적 치안의제 구성, 그리고 그 구체적 실천 양상 들을 고찰하였다. 그럼으로써 이슬람적 치안에 작용하는 권력관계와 이해관계를 파악하고, 이슬람적 치안에 의해 새롭게 조율되는 개인과 사회 간의 관계 설정 방향을 가늠해 보았다. 이러한 작업들을 통해 본고는 개혁시기 인도네시아 사람들이 경험하고 있는 사회적 스트레스들이, 지구화 시대의 이념들과 이슬람을 매개로 치안화하는 과정을 연구한 것이며 이슬람적 치안을 통해 디자인되고 있는 인도네시아 사회의 미래 전망을 고찰한 것이다.