• 대한한의학회지
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• 제24권2호
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• pp.66-80
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• 2003

Objectives : Recently, herbal acupuncture has been developed in the Korea since the earlier 1960' applied to various diseases including the cerebrovascular accident. The main characteristics of herbal acupuncture are a combination the merit of acupuncture and herbal medicine. It was not well known the therapeutic effect and the mechanism of herbal acupuncture on transient forebrain ischemic injury, although it has been used frequently in clinics. Ramulus Uncariae Cum Uncis (釣鉤藤) has been claimed to possess sedative, anti-spasmodic actions, and treat childhood epilepsy in oriental medical preparation. Also, it has been stated that Ramulus Uncariae Cum Uncis was antioxidatvie effect and neuroprotection against glutamateinduced neuronal death. Methods : In this study, effects of Ramulus Uncariae Cum Uncis' herbal acupuncture on the $GV_{20}{\;}or{\;}LR_3$, named Baek-hue or Tae-chung, on neuroprotection after the transient forebrain ischemia were investigated in Sprague-Dawely rats. Expressions of cFos, FosB and BDNF protein in the hippocampus and cortex were observed at 2 hrs and 48 hrs after transient forebrain ischemia by immunohistochemistry and ELISA technique. Results : Expression of cFos protein was increased slightly in the hippocampus and cortex at 2 hrs after transient forebrain ischemia, but FosB protein was increased highly comparing to cFos protein. However, pretreatment with Ramulus Uncariae Cum Uncis' herbal acupuncture on $GV_{20}{\;}or{\;}LR_3$ significantly increased expression of cFos protein and significantly decreased expression of FosB protein compared to control group, respectively. These features were observed in the motor cortex and retrosplenial granular cortex as well as the hippocampus. Especially, cFos expression was more increased at the herbal acupuncture on $GV_{20}{\;}than{\;}LR_3$, but FosB expression was more decreased in $LR_3$ group than $GV_{20}$ group. Also, pretreatment with Ramulus Uncariae Cum Uncis' herbal acupuncture on $GV_{20}{\;}or{\;}LR_3$ significantly increased the expression of BDNF protein in the hippocampus ($254.88{\pm}12{\;}pg/ml{\;}in{\;}GV_{20}$ group, $245.93{\pm}44.4{\;}pg/ml{\;}in{\;}LR_3$ group) and the cortex ($85.81{\pm}3.45{\;}pg/ml{\;}in{\;}GV_{20}$ group, $111.51{\pm}15.79{\;}pg/ml{\;}in{\;}LR_3$, group) compared to the hippocampus ($134.07{\pm}2.96{\;}pg/ml$) and the cortex ($61.16{\pm}4.11{\;}pg/ml$) in control group at 48 hrs after transient forebrain ischemia. Conclusion : These results suggest that pretreatment with Ramulus Uncariae Cum Uncis' herbal acupuncture on $GV_{20}{\;}or{\;}LR_3$ has neuroprotective effect on transient forebrain ischemia and the herbal acupunture on $GV_{20}{\;}or{\;}LR_3$ may be related to antioxidative effect and calcium channel block of Ramulus Uncariae Cum Uncis. Also, it could be mentioned there is specificity of acupoints treating ischemic injury through the difference between the herbal acupuncture of $GV_{20}{\;}and{\;}LR_3$.

• 대한불안의학회지
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• 제7권1호
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• pp.48-53
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• 2011

본 연구는 주요우울증 환자군과 정상 대조군을 대상으로, 한국어판 Beck 우울설문지 2판(Beck Depression Inventory-II)에 대한 신뢰도와 타당도를 검증하여 실제적이고 표준화된 규준을 제시하고, 분할점(cut-off score)을 설정하여 우울증의 유무를 나타내는 기준을 마련하고자 하였다. 주요한 연구결과는 다음과 같다. 첫째, 한국판 BDI-II의 문항-총점 상관의 범위는 .51에서 .74이고, 전체 문항들이 .60 이상의 문항-총점 상관을 보여 한국어판 Beck 우울설문지 2판의 신뢰도가 '보통' 이상이라는 것이 검증되었다. 둘째, 집단별 BDI-II의 내적 일치도를 알아보기 위한 Cronbach' alpha 값은 .94 이상인 것으로 나타났다. 셋째, 주성분 분석 방식으로 요인을 추출하여 합치성을 검사한 결과 3개의 하위 요인이 추출되었고, 설명되는 변량은 총 47.3%였다. 넷째, 한국어판 BDI-II의 분할점수 산출을 위하여 ROC(Receiver operator characteristic) 분석을 실시한 결과 18점일 때 가장 높은 민감도와 특이도를 보인 것으로 나타났다. 따라서 본 연구결과를 바탕으로, 한국어판 BDI-II의 분할점은 18점으로 정하는 것이 타당할 것으로 여겨진다. 그러나 본 연구에서는 연구에 사용된 환자군이 우울증상을 가진 모든 정신질환자를 대상으로 한 것이 아니라 주요우울증 환자들만을 대상으로 하여 결과를 일반화 하는데 일부 제약이 따를 수 있을 것으로 판단되며, 증상의 중증도(severity)에 따라 군을 나눠 분할점을 산출하지는 못하여 추후에는 이를 고려한 추가적인 연구가 필요할 것이다.