• 한국잠사곤충학회지
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• 제45권2호
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• pp.121-125
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• 2003

Dissolution of Antheraea pernyi silk fiber was carried out in a zinc nitrate 6 hydrate (Zn(NO$_3$)$_2$ㆍ6$H_2O$) solution with various dissolving conditions. The solubility was significantly dependent on the concentration of zinc nitrate, dissolving temperature and time. Regenerated A. pernyi silk fibroin powder was obtained through dialysis process to remove chaotropic salt. FTIR and X-ray diffractometer showed that the conformation of regenerated A. pernyi silk powder was sheet structure.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.95-100
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• 2011

The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels, and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.

• 한국잠사곤충학회지
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• 제51권1호
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• pp.15-19
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• 2013

작잠은 가잠과는 종속이 다른 섬유 곤충으로 우수한 섬유용 재료를 만든다. 최근 비섬유용 소재로 작잠 견단백질을 활용하기 위한 기초 연구의 일환으로 작잠 견단백질을 용해 조건에 대하여 연구 결과 작잠견사의 연감율은 16.8%로 나타났으며, 작잠견사는 염화칼슘 에탄올 혼합 용매에는 용해되지 않으나 질산칼슘수화물의 용융액에는 용해되었다. 질산칼슘과 에탄올의 조성비를 조정하여 낮은 농도의 질산칼슘 농도에서도 작잠견사가 용해되었다. 작잠 실크단백질의 주요 아미노산 조성은 알라닌, 글리신, 세리신으로 작잠 정련사와 유사한 조성을 보였다. 따라서, 금후 작잠 견단백질의 적절한 용해시스템 확보 및 소재개발을 위한 기초 자료로 활용될 수 있을 것으로 사료된다.

• 한국잠사곤충학회지
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• 제44권2호
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• pp.87-92
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• 2002

염화아연에 의한 작잠 견사의 용해 조건을 살펴본 후 투석법에 의하여 얻은 작잠 견피브로인 분말의 형태와 적외선 분광 특성 및 X-선 회절 특성을 살펴본 결과 다음과 같은 결론을 얻었다. 1. 작잠 견사는 고농도의 염화아연 수용액에 용해되었으나 용해 조건을 조절하여 완전하게 작잠 견사를 용해할 수 있었다. 2. 재생 작잠 분말의 구조 특성을 살펴보기 위하여 적외선 분광분석과 X-선 회절분석을 행한 결과 작잠분말은 $\beta$-sheet 구조와 $\alpha$-helix 구조로 이루어져 있었다. 3. 염화아연을 사용하여 제조한 작잠 견피브로인 용액으로부터 순수한 작잠 견피브로인을 얻기 위한 새로운 방법이 개발되어야 함을 알 수 있었다.

• Journal of Life Science
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• 제10권1호
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• pp.10-13
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• 2000

To develope the genetic source of oak wild silkworm, Antheraea yamamai, the cDNA library was constructed with poly A+ mRNA isolated from posterial silk gland of fifth instar larvae. Titer of the cDNA library was about 5.1$\times$105 pfu in total. We presumed that the titer covered almost all transcripts existed in Antherea yamamai. From cDNA library of Antheraea yamamai, fibroin heavy chain gene, which is specifically expressed from posterial silk gland of Antheraea yamamai, was screened using oligonuclotide probe specific to alanine rich motif of fibrin heavy chain gene of Antheraea pernyi. As a result, fibroin clones isolated from 5$\times$104 plaques showed the highest homolgy (95%) with that of Antherea pernyi in nucleotide of Anthereaea yamamai and Bombyx mori shows that there is no homologous sequence in the 3+ partial 채야후 region Genomic southern hybridization suggested that one copy is present. Northern hybridization showed that fibroin transcript was approximateely 9 kb in length.

• 한국잠사곤충학회지
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• 제42권2호
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• pp.114-119
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• 2000

Effects of ethanlo treatment on the structural and thermal characteristics of regenerated Antheraea pernyi silk fibroin (RSF) were investigated. Infrared spectroscopy and X-ray diffractometry showed that the conformational transition of RSF might be affected by concentration of ethanol and its treatment time. The structure of RSF was rapidly changed from random coil to $\beta$-sheet conformation when RSF was treated with les than 75% ethanol concentration. However, RSF treated with ethanol(100%) did not show conformational change. Differential scanning calorimetry showed that exotherm at 232$\^{C}$ disappeared and the intensity of endotherm at 228$\^{C}$ decreased with treatment of 75% ethanol. Dynamic thermal analysis showed that loss modulus (E") and tan $\delta$$\_$E/ of RSF treated with aqueous ethanol was broaden and shifted to higher temperature in comparison with those of untreated RSF.

• Fibers and Polymers
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• 제7권4호
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• pp.333-338
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• 2006

Silk fibroin (SF) fiber from the Antheraea pernyi silkworm was treated with a 1.23 N iodine-potassium iodide ($I_2-KI$) aqueous solution, and the structure and physical properties were investigated to clarify the effects of the iodine treatment. The noticeably high weight gain value of SF fiber, about 25 wt% was attributed to the absorption of polyiodide ions in the form of $I_3{^-}\;and\;I_5{^-}$. Fourier transform infrared spectroscopy and X-ray diffraction measurements suggested that polyiodide ions mainly entered the amorphous region. In addition, a new sharp reflection on the meridional direction, corresponding to a period of $7.0{\AA}$, was observed and indicated the possibility of the formation of mesophase structure of ${\beta}$-conformation chains. Dynamic viscoelastic measurements showed that the damping tan ${\delta}$ peak at $270^{\circ}C$ gradually shifted to lower temperature in the iodinated SF fibers, suggesting an enhancement of the molecular motion of the fibroin chains induced by the presence of polyiodide ions. With heating above $254^{\circ}C$, the iodine component introduced intermolecular cross-linking of SF, and the melt flow of the sample was inhibited. The thermal decomposition stability of fibroin molecules was greatly enhanced by iodine treatment.

• 한국잠사곤충학회지
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• 제41권1호
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• pp.54-60
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• 1999

염산 또는 수산화나트륨 수용액의 농도, 온도, 시간 등 처리조건에 따른 작잠견사의 가수분해율을 측정하였으며 산처리 작잠분말과 알칼리처리 작잠분말의 형태 및 구조특성을 주사전자현미경, 적외선 분광분석, X-선 회절분석 등의 방법으로 분석한 결과 다음과 같은 결과를 얻었다. 1. 작잠견사에 대한 염산 및 수산화나트륨의 처리농도와 온도가 높을수록 또한 처리시간이 길어질수록 작잠견사의 가수분해율은 증가하는 경향을 나타내었으나 고온, 고농도의 조건하에서 수산화나트륨을 작잠견사에 처리하는 경우 작잠견사의 가수분해율이 오히려 떨어지는 경향을 보였다. 2. 산 및 알칼리 처리에 의하여 제조된 작잠분말의 형태는 용해도가 30 wt% 부근에서는 섬유상의 형태를 유지하였으며 산처리 분말의 경우 용해도가 증가함에 따라 섬유축 방향으로 조각난 미세한 입자형태를 나타내었으나 알칼리처리 분말의 경우에는 부정형의 입자형태를 나타내었다. 3. 적외선 분광분석 결과 작잠분말은 처리조건과 무관하게 ${\beta}$-sheet 구조의 특성을 반영하는 1635, 1532, 1226, 960, 700 cm$^{-1}$ 흡수대와 ${\alpha}$-helix 구조의 특성을 반영하는 616 cm$^{-1}$ 흡수대가 뚜렷하게 나타나 작잠분말의 주요 conformation은 ${\beta}$-sheet 구조와 ${\alpha}$-helix 구조임을 확인하였다. 4. X-선 회절분석 결과 가수분해율이 낮을 경우 (35 wt%)에는 ${\beta}$-sheet 구조와 ${\alpha}$-helix 구조에 기인하는 회절 피크가 뚜렷하게 나타났으나 가수분해율이 80 wt% 이상으로 증가하면 2${\theta}$=17.1$^{\circ}$ 및 20.0$^{\circ}$의 회절피크만 강하게 나타났다.

• 생명과학회지
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• 제10권2호
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• pp.188-195
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• 2000

In order to understand molecular events during silk synthesis and provide genetic resources for molecular breeding, we had analyzed the cDNA library constructed from the posterior silkgland of Antheraea yamamai and partially sequenced 276 randomly selected genes from the cDNA library. Database comparisons of the expressed sequence tags (ESTs) revealed that 26 non-redundant clones showed a high similarity with previously identified genes. Among them, 17 clones exhibited a homology with previously identified insect genes and 9 clones were identical to genes that were previously identified from other organisms. A functional categorization showed that silk synthesis-defense- or stress-related genes, as well as genes involved in the metabolic pathways and in the transcriptional or translational apparatus are represented. In this report, the clone (AY479) which had high similarity with fibroin from A. pernyi was particularly analyzed in detail. The AY479 clone was carboxyl terminal region of fibroin. The 472 bp cDNA has 123 amino acids that shared 85% homology with the fibroin from A. pernyi and its deduced peptide had unique feature, that is, sites of alanine rich residues.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제36권3호
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• pp.111-115
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• 2014

Purpose: This study evaluated powdered burn wound dressing materials from wild silkworm fibroin in an animal model. Methods: Fifteen rats were used in this experiment. Full-thickness $2{\times}2cm$ burn wounds were created on the back of rats under anesthesia. In the two experimental groups, the wounds were treated with two different dressing materials made from silkworm fibroin. In the Control Group, natural healing without any dressing material was set as control. The wound surface area was measured at five days, seven days and 14 days. Wound healing was evaluated by histologic analysis. Results: By gross observation, there were no infections or severe inflammations through 14 days post-injury. The differences among groups were statistically significant at seven days and 14 days, postoperatively (P<0.037 and 0.001, respectively). By post hoc test, the defect size was significantly smaller in experimental Group 1 compared with the Control Group and experimental Group 2 at seven days postoperatively (P=0.022 and 0.029, respectively). The difference between Group 1 and Group 2 was statistically significant at 14 days postoperatively (P<0.001). Group 1 and control also differed significantly (P=0.002). Group 1 showed a smaller residual scar than the Control Group and Group 2 at 14 days post-injury. Histologic analysis showed more re-epithelization in Groups 1 and 2 than in the Control Groups. Conclusion: Burn wound healing was accelerated with silk fibroin spun by wild silkworm Antheraea pernyi. There was no atypical inflammation with silk dressing materials. In conclusion, silk dressing materials can be used for treatment of burn wound.