• The Korean Journal of Zoology
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• v.33 no.1
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• pp.28-34
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• 1990

The ipsilateral dorsal lobe of the brain one or two days after cutting a left antenna in Pieris rapae has been examined with electron microscope to investigate the connection of the receptor cells between antenna and dorsal lobe. The proximal removal of the left antenna leads to the weakly-dark, semidark or dark degeneration of antennal receptor tenninals in ipsilateral dorsal lobe. Therefore, it is concluded that some of antennal receptor cells which project into the brain terminate in ipsilateral dorsal lobe located immediately behind the antennal lobe.

• The Korean Journal of Zoology
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• v.31 no.4
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• pp.334-343
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• 1988

Five types of synaPses were differentiated in the antennal lobe or Pieris rapoe. aev are in general axo-dendritic synapses and have symmetrical contacts. The type I synapses contain the uniformly round vesicles of medium size, and consist of the 11% of all synapses in the antennal lobe. The type synapses are filled with the small flat and densely arranged (19%). The type III synapses possess medium-sized round vesicles and dense core ones(24%). The type IV synapses are characterized by such three kinds of vesicles as small round, small flat and dense core vesicles(33%). The type V synapses exhibit the presence of medium-sized round, large round and dense core vesicles(13%). The removal of the left antenna on its proximal portion caused the type IV boutons of the above ave types in ipsilateral antennal lobe to turn into dark or semidark degenerative changes. Therefore, it was concluded that many of the antennal receptor cells projected into the brain terminate in the antennal lobe to form type IV synapses together with the dendrites. 배추회나비 뇌의 촉각엽에 있는 신경세포들은 5가지의 신경연접을 형성하는 것으로 관찰되었다. 이 신경연접들은 모두 축색과 수상돌기간의 연접으로 대칭연접이었다. 제1형 신경연접은 축색종말내에 중등도크기의 단형 연접소포를 가지고 있었고, 촉각엽에 있는 5가지의 신경연접중 11%의 출현빈도수로 관찰되었다. 제2형 신경연접의 축색종말에는 장형과 도형의 2가지 연접소포가 포함되어 있었는데, 모두 소형이었으며 이같은 연접소포들이 축색 종말내에서 조밀하게 관찰되었다. 제2형 신경연접의 출현 빈도수는 19%이었다. 제3형 신경연접은 축색 종말내에 중등도크기의 도형 연접소포와 DENSE CORE VESICLE을 포함하고 있었고 그 출현빈도수는 24%이었다. 제4형 신경연접은 축색총말내에 소형의 도형, 소형의 장형 연접소포 및 DENSE CORE VESICLE의 3가지 연접소포를 가지고 있었고 출현빈도수는 촉각엽에서 가장 큰 33%이었다. 제5형 신경연접은 축색종말내에 중등도크기의 원형, 대형의 원형연접소포 및 DENSE CORE VESICLE을 포함하였고 13%의 출현빈도수로 관찰되었다. 배추횐나비의 촉각에 있는 지각신경세포가 뇌의 촉각엽으로 뻗어 들어가 위의 5가지 신경연접중 어느 형을 형성하는지를 관찰하기 위하여 좌측 촉각의 기부를 제거하여 지각신경세포를 절단하였는데 그 결과, 좌측 촉각엽에서 제4형의 신경연접이 퇴행성 변화를 나타내었다. 그러므로 촉각의 지각신경세포는 뇌의 같은 족 촉각엽에 뻗어와 제4형 신경연접을 형성한다고 결론되었다.

• The Korean Journal of Zoology
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• v.32 no.1
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• pp.1-8
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• 1989

Non-diapausing larvae and diapausing pupae of Pieris rapae L. were treated with 20-HE and JH, and the concentration of 20-HE and JH in body were also measured to determine mechanisms of diapause induction and termination. When early last instar larvae of non-diapausing stage were injected with 20-HE or JH-I, diapause was not induced in both cases. 20-HE concentration of pre-diapausing last instar larvae was very low, and 20-HE peak of non-diapausing two-day-old pupae was not present in diapausing pupae. There was no difference in concentration of JH-I between diapausing and non-diapausing stages. When early and late diapausing pupae were injected with 20-HE, JH-I, or JH-III. diapause was terminated by 0.1-0.5 $\mu$g of 20-HE, but not by JH-I, or JH-III. The above results indicate that diapause of Pieris rapae was induced by decrease of 20-HE concentration and also terminated by gradual accumulation of 20-HE during diapause process.