• Advances in Traditional Medicine
• /
• 제8권3호
• /
• pp.260-265
• /
• 2008

Annona reticulata belonging to the family Annonaceae is traditionally used as anthelmintic and it is reported to have anticancer activity. Free radicals are known to be involved in various types of diseases like cancer, diabetes, neurological disorders etc. The present study was aimed to evaluate free radical scavenging property of methanol extract of the leaves of Annona reticulata in some in vitro models e.g. DPPH, nitric oxide, superoxide and lipid peroxide radical model. The extract showed good dose-dependent free radical scavenging property in all the models. $IC_{50}$ values were found to be 3.22, 170.01, 25.12 and $140.12\;{\mu}g/ml$ respectively in DPPH, nitric oxide, superoxide and lipid peroxidation inhibition assays. Reductive ability of the extract was also tested based on potassium ferricyanide reduction where dose dependant reducing capability was observed. Measurement of total phenolic compounds by Folin-Ciocalteu's phenol reagent indicated that 1mg of the extract contained $146.20\;{\mu}g$ equivalent of pyrocatechol. The findings ascertain promising free radical scavenging property of the extract and the antioxidant property of the extract may be due to the high content of phenolic compounds.

• Journal of Applied Biological Chemistry
• /
• 제53권1호
• /
• pp.21-24
• /
• 2010

커스타드애플(Annona reticulata L.)씨 추출물 및 그의 분획물을 활용하여 복숭아혹진딧물 및 벼멸구를 대상으로 살충활성을 조사하였으며, 그 결과 아세톤/메탄올 조추출물 $3000\;{\mu}g/mL$에서 복숭아혹진딧물은 100%, 벼멸구에 대해서는 92%의 살충률을 나타내었다. 이들에 대한 $LD_{50}$는 각각 $446.3\;{\mu}g/mL$와 $1423.6\;{\mu}g/mL$로 복숭아혹진딧물이 추출물에 높은 감수성을 나타내었다. 이들의 핵산, 클로로포름, 물 분획물에 대한 분리정제물의 경우 클로로포름 분획물이 조추출물 분획물보다 약 3배 높은 살충능이 관찰되었으며, 이는 극성분배를 통해 살충성이 높은 물질군을 선별적으로 정제할 수 있음을 보여준다. 특히 클로로포름 분획물에 대한 살충능의 경우 $137.2\;{\mu}g/mL$(복숭아혹진딧물), $1145.5\;{\mu}g/mL$(벼멸구)의 농도에서 $LD_{50}$가 관찰되었다.