• Title/Summary/Keyword: Animal and Plant Quarantine Agency

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Establishment of optimal disinfection condition of weak acid hypochlorous solution for prevention of avian influenza and foot-and-mouth disease virus transmission (조류 인플루엔자와 구제역 바이러스 차단방역을 위한 미산성 차아염소산수의 소독 조건)

  • Kim, Jin-Yoon;Yun, Dong-Sik;Lee, Haw-Yong;Jeong, Woo-Seog;Park, Seung-Chun
    • Korean Journal of Veterinary Research
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    • v.59 no.2
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    • pp.101-104
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    • 2019
  • This study examined the disinfection conditions (exposure time, 0-30 min; exposure temperature, $4^{\circ}C-65^{\circ}C$) of hypochlorous acid water (HOCl) in automobile disinfection equipment. The study tested poliovirus type 1 (PV1), low pathogenic avian influenza virus (AIV, H9N2), and foot and mouth disease virus (FMDV, O type). As a result, the PV1 and FMD viruses were inactivated easily (virus titer 4 log value) by HOCl (> 100 ppm) but the AIV required higher exposure temperatures (> $55^{\circ}C$). In conclusion, the exposure temperature and time are important factors in deactivating AIV and FMDV.

Distribution of Gastrointestinal Parasitic Infection in Domestic Pigs in the Republic of Korea: Nationwide Survey from 2020-2021

  • Lee, Subin;Alkathiri, Badriah;Kwak, Dongmi;Lee, Sang-Myeong;Lee, Wan-Kyu;Byun, Jae-Won;Lee, Seung-Hun
    • Parasites, Hosts and Diseases
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    • v.60 no.3
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    • pp.207-211
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    • 2022
  • This study aimed to examine the distribution of gastrointestinal parasitic infections in domestic pigs in the Republic of Korea. From May 2020 to October 2021, 364 pig fecal samples were collected from 75 farms in 7 Provinces and microscopically examined. A total of 170 (46.7%) pigs were infected with at least one of the following parasites: Balantioides coli, strongyles, Ascaris suum, Trichuris suis, and coccidia. By parasite species, B. coli, strongyles, A. suum, T. suis, and coccidia oocysts or eggs were detected in 144 (39.6%), 24 (6.6%), 14 (3.8%), 4 (1.1%), and 1 (0.3%) samples, respectively. One hundred fifty-four, 15, and 1 cases showed single, double, and triple infections, respectively. Of the swine fecal samples from 75 farms, 69 specimens (92.0%) were infected with 1 or more parasites. All surveyed farms across the country exhibited a positive rate of over 30%, among which the highest positive rate was 65.0% in Chungcheongnamdo, and Jeollabuk-do was followed by 61.9%. Winter showed a statistically lower prevalence than other seasons. This study showed that gastrointestinal parasites are prevalent in pigs in Korea, although the diversity of parasites is low.

The European Pepper Moth, Duponchelia fovealis Zeller (Lepidoptera: Crambidae) Discovered in Gyeonggi-do, Korea (한국에서 Duponchelia fovealis Zeller의 발견보고)

  • Lee, Young Su;Park, Young Mi;Lee, Gwan-Seok;Lee, Hee A;Min, Hee OK;Lee, Hyun Ju
    • Korean journal of applied entomology
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    • v.57 no.1
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    • pp.53-54
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    • 2018
  • The European pepper moth, Duponchelia fovealis Zeller (Lepidoptera: Crambidae) was discovered at Gyeonggi-do, Korea in 2015 and 2016. The moth has been known as a very harmful pest of ornamental plants worldwide. Larval damages to the Euphorbia milii stems and to the Schlumbergera truncata leaves were detected. After immediate spraying of insecticides, the moth was not found at the greenhouse any more.

Genetic Characterization of an Ancestral Strain of the Avian-Origin H3N2 Canine Influenza Virus Currently Circulating in East Asia

  • Kim, Jeong-Ki;Nam, Jeong-Hyun;Lyoo, Kwang-Soo;Moon, Hyoungjoon;Na, Woonsung;Song, Eun-Jung;Yeom, Minjoo;Shim, Sang-Mu;Jeong, Dae Gwin;An, Dong-Jun;Kang, Bo-Kyu;Song, Daesub
    • Journal of Microbiology and Biotechnology
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    • v.26 no.6
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    • pp.1109-1114
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    • 2016
  • H3N2 canine influenza virus emerged in South Korea in 2007 and subsequently spread to China and Thailand, causing epidemic or endemic respiratory diseases in dogs. Through intermammalian species transmission, the virus has also infected cats. However, no direct evidence of significant genetic evolution has been reported since its first emergence. Here, we describe in depth the genetic and molecular characteristics of the ancestral strain (i.e., the first virus isolate from South Korea) of the H3N2 canine influenza virus currently circulating in East Asia.

Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea (경북 동부지역 꿀벌에서 주요 병원체의 분자생물학적 검출)

  • Ouh, In-Ohk;Do, Jae-Cheul;Seo, Min-Goo;Jeong, Tae-Nam;Cho, Min-Hee;Kwak, Dong-Mi
    • Korean Journal of Veterinary Service
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    • v.36 no.1
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    • pp.37-44
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    • 2013
  • The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.

Studies on the overinflation of the Cardiac Stomach in Sterlet Sturgeon Acipenser ruthenus, fingerlings (양식 스털렛 철갑상어, Acipenser ruthenus 치어의 위 팽창 증후군에 관한 연구)

  • Park, Sung-Woo;Yu, Jin-Ha;Han, Jung-Jo
    • Journal of fish pathology
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    • v.25 no.1
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    • pp.59-65
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    • 2012
  • Overinflation of cardiac stomach occurred in farmed sterlet sturgeon (Acipenser ruthenus) fingerlings. Affected fish congregated and swam upside-down near the edges of the tanks with abdominal distension. When pressed the dilated abdomen, the fish often exhibited a normal swimming pattern for some time, then resumed their characteristic upside-down swimming patterns. Internally, the fish showed abdominal dropsy and dilated stomach filled with yellowish granular contents. When the contents in the stomach, fish feed, water and excrement in the farm inoculated on Sabouraud destrose agar (SDA) at $25^{\circ}C$ for 48 hr. Candida sp. were isolated except from the feed. Candida sp. in excrement or water in the farm may enter into the stomach with feed and then produce gases in the metabolic process, which in turn may cause the stomach overinflation.

Serotype and antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolates from pigs in Korea (돼지에서 분리한 Actinobacillus pleuropneumoniae의 혈청형 분포 및 항생제 감수성)

  • Jung, Ji-Youl;Jang, Hyun
    • Korean Journal of Veterinary Research
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    • v.52 no.3
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    • pp.177-181
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    • 2012
  • Actinobacillus (A.) pleuropneumoniae is the causative agent of pleuropneumonia which is one of the most important respiratory diseases in pigs worldwide. A total of 32 A. pleuropneumoniae isolates from diseased pigs during 2008 to 2010 were serotyped by polymerase chain reaction method. The susceptibility of the isolates to 13 antimicrobial agents were determined by disk diffusion test. In all the 32 isolates examined in this study, serotype 5 (16 isolates: 50%), 1 (7 isolates: 21.9%), 2 (5 isolates: 15.6%) and 12 (1 isolate: 3.1%) were found. Of all tested antimicrobial agents, resistance to oxytetracycline was found in 96.9% of isolates, followed by resistance to amikacin (81.2%), neomycin (68.7%), kanamycin (53.1%), penicillin (50.0%), gentamicin (43.7%), florfenicol (25.0%), ampicillin (18.7%), colistin (9.4%), trimethoprim/sulfamethoxazole, ceftiofur (8.3%), amoxicillin/clavulanic acid (3.1%) and enrofloxacin (0%). Oxytetracycline or florfenicol-resistant isolates were examined for the presence of resistance gene. Among the 31 oxytetracycline-resistant isolates, tetB, tetH and tetO genes were detected in 22 (71%), 8 (26%) and 1 (3%) isolates, respectively. The floR genes were detected in 8 (100%) of the 8 florfenicol-resistant A. pleuropneumoniae isolates.

Patterns of antibiotic resistance in Escherichia coli isolated from fresh and recycled poultry litter (깔짚 교체 및 재사용 육계농장 분리 대장균의 항생제 내성 양상)

  • Sung, Haan-Woo;Choi, Kang-Seuk;Kwon, Hyuk-Moo;Lee, Young-Ju
    • Korean Journal of Veterinary Research
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    • v.57 no.3
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    • pp.189-195
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    • 2017
  • The isolation rate of Escherichia (E.) coli in poultry litter was investigated at 44 broiler farms, 20 that used fresh litter and 24 that used recycled litter. The patterns of resistance to antibiotics of the E. coli isolates were compared. In litter sampled before the rearing period, the isolation rate of E. coli was higher at farms that used fresh litter; E. coli was present in the litter in 94.5% (35 out of 37 flocks tested) of the farms that used fresh litter vs. 51.2% (21 out of 41 flocks) of the farms that used recycled litter. The susceptibility of the 93 isolates of E. coli to 13 antibiotics was studied. Before the rearing period, E. coli isolates from the farms that recycled litter showed higher resistance rates than isolates from farms that replaced litter with fresh litter. Comparing the antibiotic resistance patterns of isolates from litter sampled before and at the end of the rearing period, the antibiotic resistance rates at the end of the rearing period increased dramatically compared with rates before the rearing period.

Thelohanellus misgurni (Kudo, 1919) infection on the fins of Chinese muddy loach Misgurnus mizolepis (미꾸라지, Misgurnus nazolepis의 지느러미에 기생한 포자충, Thelohanellus misgurni (Kudo, 1919)에 관하여)

  • Kwon, Se-Ryun;Kim, Hyoung-Jun
    • Journal of fish pathology
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    • v.24 no.2
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    • pp.167-171
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    • 2011
  • On September in 2010, myxosporidian parasites were found on the fins of Chinese muddy loach, Misgurnus mizolepis, Several cysts of approximately 2 mm diameter were observed on the fins. Numerous spores were found in the cyst. The total length of the spore was 13.0 to 15.8 ${\mu}m$, and the width of spore was 6.0 to 7.5 ${\mu}m$. As the results, the myxosporidians were finally identified as Thelohanellus misgurni (Kudo, 1919) on the basis of the presence of characteristic cysts and size of spores.

A case of using a vibrating saw to collect brain tissue for BSE (bovine spongiform encephalopathy) test in Holstein (홀스타인에서 BSE (bovine spongiform encephalopathy) 검사용 뇌 조직 채취를 위한 진동톱 사용례)

  • Lee, Jong-Won;Lee, Eun-Mi;Lee, Jung-Woo;Jung, Eun-Hye;Do, Jae-Cheul;Hur, Sung-Eun
    • Korean Journal of Veterinary Service
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    • v.39 no.3
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    • pp.205-210
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    • 2016
  • BSE (bovine spongiform encephalopathy)-like symptoms salivation, gait disorder, and the downer syndrome occurred to 8 heads of cattle at six cattle farms in Gyeonju, east eastern Gyeongsang province in 2013. Eight brain tissue samples using a vibration saw (Multimaster$^{(R)}$ FEIN, Germany) were collected referring to "The brain removal technique of transmisible spongiform encephalopathy sampling DVD" by Animal And Plant Quarantine Agency. Brain removal techinque using a vibration saw on the thick, solid frontal bone of cattle is more convenient for incision and removal than an ax for BSE testing.