• 한방재활의학과학회지
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• 제25권1호
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• pp.45-52
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• 2015

Objectives To check marker content for appropriate quality control of Woohwangchungsimwon sterilized to ensure microbiological safety and to observe antioxidant activity for any changes in efficacy. Methods To measure any effects of sterilization on the effective compounds, 8 ingredients of Woohwangchungsimwon were screened for any changes in marker content using HPLC-DAD. Using the colorimetric method on the microplate reader any changes in total phenolic compound and flavonoid levels were observed. Antioxidant activity was measured using the DPPH, ABTS, and FRAP. Results Of the ingredients of Woohwangchungsimwon, 8 were subject to quantitative analysis before and after sterilization. 21.6 mg and 1.93 mg of Glycyrrhizin was found in Glycyrrhiza uralensis Fischer pre and post sterilization, respectively. Decursin found in Angelica gigas Nakai increased from 0.16 mg to 0.29 mg after sterilization. Bilirubin found in Gallstone of Bostaurusvar. domesticus increased from 0.24 mg to 0.33 mg. Cinnamic acid found in Cinnamomum cassia Blume increased from 0.02 mg to 0.05 mg. Ginsenoside Rb1 found in Panax ginseng C. A. Meyer decreased from 0.02 mg to 0.14 mg. Paeoniflorin found in Paeonia lactiflora Pallas increased from 1.05 mg to 1.13 mg. Amygdalin found in Armeniacae Amarum Semen increased from 2.68 mg to 2.83 mg. L-muscone found in Musk increased from 0.63 mg to 0.76 mg. As for total phenolic compound and total flavonoid content, there was a 1.22 and 4.15-fold increase. DPPH and ABTS increased by 20.45% and 20.69%, respectively. FRAP activity was 2.78 times more active post stabilization. Conclusions This study confirmed that high temperature high pressure steam sterilization, a method used to ensure microbiological safety of Woohwangchungsimwon, does not affect marker content; in other words, does not affect quality of the Woohwangchungsimwon. It could also be seen that total phenolic compound and flavonoid content increased after sterilization. An antioxidant activity test showed that there was significantly increased activity of antioxidants.

• 대한화장품학회지
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• 제41권2호
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• pp.159-164
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• 2015

새로운 항염증 및 미백 소재를 찾기 위해서, 본 연구에서는 6가지 한약재 추출물(유자, 상백피, 오미자, 율무, 당귀, 고삼)을 Streptococcus thermophilus에 의해 발효한 발효분말의 미백 및 항염증 효과를 피부섬유아세포에 대한 피부독성, 산화질소 생성, tyrosinase 활성, 멜라닌 형성의 저해 효과를 측정함으로써 평가하였다. 발효물은 $37^{\circ}C$에서 2일 동안 Streptococcus thermophilus에 의해 발효한 후, 동결건조에 의해 발효분말을 제조하였다. 발효분말은 피부섬유아세포에 대해 $500{\mu}g/mL$의 농도에서 cytopathic effect reduction 방법을 사용하면서 측정하였을 때 세포독성을 나타내지 않았다. 또한 발효분말은 Griess reagent system을 사용하면서 산화질소 생성에 대한 저해 효과를 나타내었다. 더욱이 발효분말은 tyrosinase 활성에 대한 저해 효과가 농도 의존적으로 나타났다. 발효분말은 배양액으로부터 멜라닌 생성에 대해 유의적인 저해를 나타내었다(p < 0.05). 그러므로 이러한 자료로부터 발효분말은 항염증 및 미백 효과를 갖는 것으로 나타났으며, 화장품을 위한 효과적인 성분으로써 사용가능할 것으로 사료된다.

• 농약과학회지
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• 제4권3호
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• pp.60-67
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• 2000

건조된 한약재 시료인 백삼, 홍삼, 지황, 당귀 및 시호 중에 잔류되어 있는 tolclofos-methyl 및 quintozene의 추출에 microwave 추출법을 이용하고자 Soxhlet 추출법과 추출효율을 비교하여 최적의 추출용매, 추출시간 및 power supply의 강도 등에 대하여 조사하였다. 다섯 가지 한약재 시료에 잔류되어 있는 tolclofos-methyl 및 quintozene을 microwave로 추출하기 위한 용매는 acetone이 가장 효율적이었다. 추출효율은 Soxhlet 7시간 추출과 microwave로 45 watts에서 1분에서 10분 동안 추출한 결과를 비교하였는데 microwave 추출시간을 $3{\sim}5$분으로 한 결과와 Soxhlet 법으로 7시간 추출한 결과는 거의 같게 나타났다. 시료의 종류에 따른 microwave의 추출특성은 인삼은 5분, 다른 한약재들은 3분의 추출에서 Soxhlet추출과 거의 같은 추출 효율을 나타내었다. 잔류분석시 불순물의 방해를 고려한 최적 추출효율을 나타내는 microwave power는 $45{\sim}90$watts로 나타났다. 따라서 tolclofos-methyl 및 quintozene이 잔류되어 있는 다섯 가지 한약재 시료에서의 microwave를 이용한 추출의 최적 조건은 power $45{\sim}90$ watts, 추출용매 acetone, 추출시간 $3{\sim}5$분인 것으로 나타났다.

• 동의생리병리학회지
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• 제26권1호
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• pp.10-18
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• 2012

In this paper, to find out how different the treatment of special individuals named 'old people' is with that of ordinary cases, Dongeuibogam(東醫寶鑑) was chosen as the study material and some parts in it where the symptoms of old people in the same disease category were treated in different ways were excerpted to analyze the tendency of pathological symptoms and prescriptions in the physiological perspective. As the result of analysis on the parts in Dongeuibogam(東醫寶鑑) where the old people were treated in different ways, it turned out that 65 prescriptions were used in 24 pathological symptoms. The 24 symptoms are included mainly in chapters of 'inner view(內景)' and 'outer form(外形)'. They are including the pathological symptoms which had been presented as general geriatric symptoms and also seems to have added other symptoms which should be clinically reflected in the specialty of treatment for geriatric diseases. The 65 prescriptions are also recorded mainly in chapters of 'inner view(內景)' and 'outer form(外形)'. The herbs used for them were sweet and warm herbs such as Rehmannia glutinosa (Gaertner) Libosch(熟地黃), Angelica gigas Nakai(當歸), Panax ginseng C. A. Mey(人蔘) and Atractylodes macrocephala Koidz(白朮) that can support the essence blood of liver and kidney and the energy of spleen, lung and stomach. Those herbs could be added or subtracted according to the symptom. It seems to have resulted from the treatment method that old people was applied to old people in consideration of their physiological features. When the 24 symptoms and 65 prescriptions for geriatric diseases different from ordinary ones in Dongeuibogam(東醫寶鑑) were considered in the physiological view point, it could be seen that each pathological symptom was manifested as a exhaustion of kidney qi(腎氣枯渴) which was a characteristic physiologic state of old people. Through this, it could be seen that the use of prescriptions was also made to mitigate the body fluid deficiency(津液不足), the kidney yin inner heat(腎陰內熱), the heart spirit void and loss(心神虛損), the kidney yang deficiency(腎陽不足), the spleen failing in transportation(脾失健運), the spleen yang deficiency(脾陽不足), the liver fire inner movement(肝火內動) and the lung energy void and loss(肺氣虛寒).