• 한국식품과학회지
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• 제29권6호
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• pp.1113-1118
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• 1997

국내에서 재배 수확되는 토당귀와 일당귀를 한방이나 가공시에 기초 자료로 활용될 수 있도록 기본적인 화학 성분을 분석하였다. 일반성분은 건물기준으로 토당귀와 일당귀의 조단백질은 각각 18,1, 13.4%, 조지방은 8.9, 4.3%, 조섬유는 8.6, 9.4%,조회분은 7.4, 8.2%, 당질은 57.0, 64.7%이었다. 두 종류의 당귀 속에 함유된 무기질 함량은 칼륨이 각각 2,740.0, 2,582.4 mg%로 가장 높았으며, 유리당 조성은 sucrose가 각각 0.4, 0.3%, fructose와 glucose는 검출되지 않았다. 토당귀와 일당귀의 지방산 조성은 linoleic acid가 각각 60.8, 59.9%, palmitic acid는 17.4, 15.3%. oleic acid는 8.8, 7.7%이었는데 두 시료간 뚜렷한 차이점은 발견할 수 없었다. 토당귀와 일당귀의 주요 구성아미산으로 아르기닌이 각각 2599.8, 1543.4 mg% 로 가장 많았고, 티로신이 각각 113.7, 84.9 mg%로 가장 적게 함유되어 있었다. 비타민 $B_1$,는 토당귀와 일당귀에 각각 10.5, 12.2 mg%, 비타민 $B_2$,는 각각 0.1, 0.04 mg%, 지표성분인 decursin은 각각 4.3, 0.8%이었다 탄닌은 토당귀, 일당귀에서 각각 988.0, 900.0 mg%로 분석되었다.

• Applied Biological Chemistry
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• 제30권1호
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• pp.9-16
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• 1987

당귀(當歸)의 수량(收量)과 품질(品質)을 증대(增大)시키기 위한 합리적(合理的)인 3요소(要素) 시용량(施用量)을 정립(定立)하기 위하여 3요소(要素) 시용(施用) 및 흡수량(吸收量)이 decursin 및 당함량(糖含量)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같았다. Decursin 함량(含量)은 질소(窒素) 10kg/10a, 인산(燐酸) 20kg/10a, 가리(加里) 15kg/10a 시용량(施用量)까지 증가(增加)하였으며, 10a당(當) 총생산량(總生産量)은 질소(窒素) 10kg/10a, 인산(燐酸) 15kg/10a, 가리(加里) 10kg/10a까지 증가(增加)하였으나 질소(窒素) 20kg/10a 이상 시용량(施用量)에서는 오히려 감소(減少)하였다. 전당(全糖)의 생산량(生産量)은 질소(窒素)와 가리(加里)를 증시(增施)함에 따라 증가(增加)되었지만 각각(各各) 15kg/10a, 10kg/10a이상 시용(施用)할 경우에는 효과(效果)가 인정(認定)되지 않았으며, 인산(燐酸)은 시용량(施用量)에 따른 차이(差異)가 인정(認定)되지 않았다. Decursin 함량(含量)은 당귀근중(當歸根中)의 질소함량(窒素含量)과 부(負)의 상관(相關)을, 인산(燐酸) 및 가리함량(加里含量)과 정(正)의 상관(相關)을 보였으나, 지상부(地上部)의 3요소(要素) 흡수량(吸收量)과는 유의성(有意性)이 없었다. 그러므로 3요소(要素)의 시용량(施用量)을 각각(各各) 10kg/10a, 15kg/10a, 10kg/10a로 하는 것이 적당(適當)할 것으로 판단(判斷)되었다.

• 한국조리학회지
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• 제20권5호
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• pp.130-135
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• 2014

This study determined the effects of addition of medicinal herb extracts (Zanthoxylum piperitum extract(TZ), Acanthopanax sessiliflorus(TA), Panax ginseng(TP), Cinnamomum lureitri Nees(TC), Angelica gigantis radix(TAG) and Crataegii fructus extract (TCF) to teriyaki sauce with regard to taste and health benefit. After adding the medicinal herb extracts to teriyaki sauce, moisture content was found to be inversely proportional to the viscosity. The sauce with Panax ginseng extract(TP) had lowest sweetness, but highest salinity. The results showed that the sauce with Korean herb extracts had lower salinity content. In terms of colour value, the sauce with Crataegii fructus extract(TCF) had much higher lightness, redness, and yellowness than sauce prepared with other herb extracts. It had the strongest acidic taste and lowest pH. Sensory evaluation test revealed that the sauce with Anthoxylum piperitum extract(TZ) was the most preferred sauce among all the sauces tested. The degree of brown color of teriyaki sauces was correlated positively with taste preference(r=.570, p<0.01) and overall preference(r=.505, p<0.01).

• 한방재활의학과학회지
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• 제26권4호
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• pp.9-21
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• 2016

Objectives This study is to review the effect of herbal medicine on treatment of sciatic nerve injury induced animal models reported in domestic & foreign journals. Methods 5 electronic databases (Pubmed, CAJ, RISS, Oasis, Koreantk) were searched with term as sciatic nerve injury, and animal study reports on sciatic nerve impairment with herbal medicine treatment were extracted. Results 31 articles were reviewd. All studies used SD rat. 26 studies used crush injury at the sciatic nerve using haemostatic forcep and 5 studies used sciatic nerve transection. 15 studies used single herb extract and 16 studies used complex herb medicine. Angelica gigantis radix was the most frequently used herb. Each study showed significant changes of improvement indicators from sciatic nerve impairment. Conclusions Various herb medicine are expected to have positive effects on sciatic nerve impairment.

• 대한한의학방제학회지
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• 제21권1호
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• pp.131-141
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• 2013

Objectives : This study was performed to assess the ameliorative effects of Gagam-GongJin-dan (WSY-1075) composited with Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Ginseng Radix, Cervi parvum Cornu and Cinnamomi Cortex in contact dermatitis animal model. Methods : WSY-1075 was orally administrated the various concentrations (50-400 mg/kg, body weight/day) with one time per day for 10 days from 4 days after DNFB sensitization. We investigated ameliorative effects of WST-1075 on the scratching behavior, skin clinical serverity and inflammatory mediators in 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis mice. Results : The orally administration of WSY-1075 (400 mg/kg) inhibited the scratching behavior induced by sensitization and challenge with DNFB. WSY-1075 (200 mg/kg or 400 mg/kg) administration also reduced the skin damage, inflammatory mediators, mast cell infiltration induced by DNFB. Moreover, WSY-1075 (above 200 mg/kg) administration inhibited the serum levels of IgE and IL-4 in DNFB-induced contact dermatitis mice. Conclusions : These results suggest that WSY-1075 administration (200 mg/kg or 400 mg/kg) has the ameliorative effects on the scratching behavior, the clinical signs, mast cell infiltration and inflammatory mediators in DNFB-induced contact dermatitis animal model mice.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.80-103
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• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 대한한의학회지
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• 제28권2호통권70호
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• pp.93-113
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• 2007

Objective : To research Trends of studies about anti-inflammation and pain relief, obesity, and safety of herbal acupuncture therapy by analyzing domestic theses, published since 2001, about herbal acupuncture therapy. Methods : Domestic theses, published since 2001, mentioning anti-inflammation or pain relief, obesity, or safety of herbal acupuncture therapy were reviewed and analyzed. These theses were then classified by university, year, and subject. Results : The following results were obtained in this study. 1. Among published theses related to anti-inflammation effects of herbal-acupuncture, studies about arthritis comprised the most part, 52. In theses relating to causes of arthritis, 16 were about adjuvant, which was the most, followed by Type II collagen, LPS and carrageenan. Blood test, reactions of inflammation and revelation of cytokine and immune cellswere methods for evaluating anti-inflammation effect. The tendency of experimental methods was to focus on molecular biologic method. 2. In theses related to pain relief, many clinical attempts with herbal injection were carried out, and Carthami Flos and Scolopendrawere used most. Observing reduction of pain inducing factor and checking behavioral change were methods for evaluating pain relief. 3. In theses related to obesity, research focused on effects in association with spots on the body suitable for acupuncture. There were also attempts comparing effectiveness between single injections and complex injections. Astraball Radix, Angelica Gigantis Radix, Coicis Semen and Taeumjowetang were used. Evaluation of anti-obesity effects were by weight loss, food efficiency, blood lipid profile and evaluation of liver function. 4. In theses related to safety of herbal-acupuncture, Herba Chelidonii Chaenonelis Fructus, Clematis Florida Thunb, Corydalidis Tuber, Paeoniae Radix, and Carthami Flos which marked 2 theses each were most studied. Methods of evaluating safety were mostly by observing liver and kidney functions based on blood test, and by applying herbal injections to clinical treatment. Conclusion : Herbal acupuncture is being used in various ways associating with its anti-inflammation, pain relief and anti-obesity effect. Studies on efficacy and mechanism of herbal acupuncture are being conducted even at the molecular biology level.

• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.27-36
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• 2017

Angelicae Gigantis Radix (AGR, Angelica gigas) has been used for a long time as a traditional folk medicine in Korea and oriental countries. Decursinol angelate (DCA) is structurally isomeric decursin, one of the major components of AGR. This study was performed to confirm whether DCA augments pentobarbital-induced sleeping behaviors via the activation of $GABA_A$-ergic systems in animals. Oral administration of DCA (10, 25 and 50 mg/kg) markedly suppressed spontaneous locomotor activity. DCA also prolonged sleeping time, and decreased the sleep latency by pentobarbital (42 mg/kg), in a dose-dependent manner, similar to muscimol, both at the hypnotic (42 mg/kg) and sub-hypnotic (28 mg/kg) dosages. Especially, DCA increased the number of sleeping animals in the sub-hypnotic dosage. DCA (50 mg/kg, p.o.) itself modulated sleep architectures; DCA reduced the counts of sleep/wake cycles. At the same time, DCA increased total sleep time, but not non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. In the molecular experiments. DCA (0.001, 0.01 and $0.1{\mu}g/ml$) increased intracellular Cl- influx level in hypothalamic primary cultured neuronal cells of rats. In addition, DCA increased the protein expression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptors subtypes. Taken together, these results suggest that DCA potentiates pentobarbital-induced sleeping behaviors through the activation of $GABA_A$-ergic systems, and can be useful in the treatment of insomnia.

• 대한수의학회지
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• 제55권4호
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• pp.233-240
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• 2015

Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin ${\alpha}_{II}b{\beta}3$ was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases.

• 대한약침학회지
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• 제21권2호
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• pp.104-111
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• 2018

Objective:This study is to evaluate both the single-dose intravenous injection toxicity and the approximate lethal dose of Water-soluble Danggui Pharmacopuncture (WDP) in Sprague-Dawley (SD) rats. Methods: Toxicity experiments were conducted at Good Laboratory Practice (GLP) laboratory in Biotoxtech Co., according to the regulations of GLP. WDP injection of dose 0.125, 0.25, and 0.5 mL/animal were experimental groups and normal saline injection group was control group. WDP and normal saline were injected once to 6- week old 5 male and 5 female SD rats at the tail veins at approximately 2 mL/min. During 14 days after the injection, general symptoms were observed and weight were measured. After the observation period, hematological and blood biochemical examination, macroscopic autopsy, topical resistance test at the injection area were performed. Results: RThe WDP 0.5 mL/animal injection group in 4 cases of male rats and all cases of female rats showed hematuria 30 minutes after the administration. However, after 1 hour, no more abnormal general symptoms were observed. The WDP did not affect weight, hematological and blood biochemical examination, macroscopic autopsy, and topical resistance test at the injection area. Conclusion: WDP single dose intravenous injection results showed that WDP have no toxic effects and a lethal dose of WDP should be over 0.5 mL/animal in male and female rats under the study condition. So WDP may be safe.