• Korean Journal of Agricultural Science
• /
• v.43 no.1
• /
• pp.1-13
• /
• 2016

Climate changes are shifting the perception of C4 photosynthetic crops due to their superior adaptability to harsh conditions. The tribe Andropogoneae includes some economically important grasses, such as Zea mays, Sorghum bicolor, Miscanthus spp., and Saccharum spp., representing C4 photosynthetic grasses. Although the Andropogoneae grasses diverged fairly recently, their genomic structures are remarkably different from each other. As previously reported, the family Poaceae shares the pan-cereal duplication event occurring ca. 65 MYA. Since this event, Sorghum bicolor has never experienced any additional duplication event. However, some lineage-specific duplication events were reported in Z. mays and Saccharum spp., and, more recently, it was revealed that a shared allotetraploidization event occurred before the divergence between Miscanthus and Saccharum (but after the divergence from S. bicolor), which provided important clues to those two species having large genome sizes with complicated ploidy numbers. The complex genomic structures of sugarcane and Miscanthus (defined as the Saccharum complex along with some other taxa) have had a limiting effect on the use of their molecular information in breeding programs. For the last decade, genomics-associated technologies have become an important tool for molecular crop breeding (genomics-assisted breeding, GAB), but it has not been directly applied to sugarcane and Miscanthus due to their complicated genome structures. As genomics research advances, molecular breeding of those crops can take advantage of technical improvements at a reasonable cost through comparative genomic approaches. Active genomic research of non-model species using closely related model species will facilitate the improvement of those crops in the future.

• Journal of Plant Biology
• /
• v.38 no.1
• /
• pp.107-113
• /
• 1995

pRLYS1 containing intact rbcL gene of maize (Zea mays L. cv Golden X Bantam T-51; Zm-A) was digested with several restriction enzymes to construct subcones carrying promoter region of rbcL. The DNA fragments of 0.20, 0.19, 0.92 and 1.55 kb among the EcoRI digests, the EcoRI-DdeI digests, the AvaI digests and the EcoRI-BamHI digests of pRLYS1 were subcloned into pBluscriptSK＋and named pRLPS2, pRLPS3, pRLPS14 and pRLPS35, respectively. Four subclones contain the 1.92 kb portion from 136 nucleotide downstream to 1780 nucleotide upstream from the ATG initiation codon of rbcL gene. pRLPS2 (-29 to -229) and pRLPS3 (-239 to -420 from the ATG) were sequenced. When nucleotide sequence of Zm-A was compared with sequence of rbcL promoter region of a different cultivar of maize (Zea mays L. cv WFG TMS X BS7; Zm-B), the difference rate between two cultivars was 4.3%. The mean of sequence divergence between Zm-A and three grass species in the same tribe, Andropogoneae, in the upstream region from 29 to 420 of ATG was 1.8%, whereas between Zm-B and above-mentioned three species was 5.4%. Therefore, Zm-A seems to evolutionarily closer to three other species in Andropogoneae tribe than Zm-B is.

• Korean Journal of Plant Taxonomy
• /
• v.52 no.3
• /
• pp.127-143
• /
• 2022

This study was conducted to clarify the phylogenetic position and relationships of Korean Poaceae taxa. A total of 438 taxa including 155 accessions of Korean Poaceae (representing 92% and 72% of Korean Poaceous genera and species, respectively) were employed for phylogeny reconstruction. Sequence data of eight chloroplast DNA markers were used for molecular phylogenetic analyses. The resulted phylogeny was mostly concordant with previous phylogenetic hypotheses, especially in terms of subfamilial and tribal relationships. Several taxa-specific indels were detected in the molecular phylogeny, including a 45 bp deletion in rps3 (PACMAD [Panicoideae, Arundinoideae, Chloridoideae, Micrairoideae, Aristidoideae, Danthonioideae] clade), a 15 bp deletion in ndhF (Oryzeae + Phyllorachideae), a 6 bp deletion in trnLF (Poeae s.l.), and two (17 bp and 378 bp) deletions in atpF-H (Pooideae). The Korean Poaceae members were classified into 23 tribes, representing eight subfamilies. The subfamilial and tribal classifications of the Korean taxa were generally congruent with a recently published system, whereas some subtribes and genera were found to be non-monophyletic. The taxa included in the PACMAD clade (especially Andropogoneae) showed very weak and uncertain phylogenetic relationships, presumably to be due to evolutionary radiation and polyploidization. The reconstructed phylogeny can be utilized to update the taxonomic positions of the newly examined grass accessions.

• Korean Journal of Heritage: History & Science
• /
• v.42 no.3
• /
• pp.48-65
• /
• 2009

In order to understand the local vegetation history and the origin of rice cultivation, a phytoliths analysis was performed with sediments collected from archaeological site at Ga-dong in Gijang, Busan. From the analysis with the sediments of three sampling sites, fan-shape phytoliths of Oryza sativa were detected: sediment 8 ~ sediment 29 of sampling site 1, sediment 6 ~ sediment 20 of sampling site 2 and sediment 7 ~ sediment 33 of sampling site 3. On the bottom layers or near the bottom layers from the three sampling sites, high densities of Arundinoideae and Andropogoneae were detected. It indicates that these layers were wet soil environment when they were sedimented. From near bottoms up to surfaces, the densities of Arundinoideae and Andropogoneae were abruptly decreased, while the density of Oryza sativa was sharply increased. With these results, it should be considered that the rice cultivation was started from the Bronze Age on this region.

• The Korean Journal of Quaternary Research
• /
• v.25 no.2
• /
• pp.39-48
• /
• 2011

Phytolith analysis of sediments at the river mouth of valley bottom plains in Myosa-ri, Haman is performed. By the phytolith results, Phragmites and Oryza sativa are dominated in the lower horizon. An abundance of Paniceae and Andropogoneae indicates the active agricultural activities under wet conditions. Moreover, the results of archeological studies suggest the paddy fields in the Three States Age. The phytolith assemblages are dominated in the middle horizon and they gradually increase in the upper horizon. The rice cultivation layer is not proportional to total phytolith yield due to the dominance of Oryza sativa in the lower horizon. However, because the Oryza sativa are continuously observed over the entire sediments, it is suggested that there had been the agricultural activities for the entire periods.

• The Korean Journal of Quaternary Research
• /
• v.25 no.1
• /
• pp.31-39
• /
• 2011

Phytolith of soil in earthenware buried in the wooden chamber tombs at the archaeological site in Hwangseong-dong, Gyeongju along Hyeongsan River are analyzed. The study area has adjoined the seat of government during approximately thousand years in Shilla Dynasty and can be regarded as being closely related to the agricultural activities using the floodplains of Hyeongsan River. By the analytical results, Phragmites, Paniceae and Andropogoneae as well as Oryza sativa are found in the lower horizons, indicating the agricultural activities in the nearby areas. It is suggested for understanding the use of earthenware that the comparisons of buried soils in the site and soils in the earthenware and analysis on the macro plant fossils of soil in the earthenware are needed.