• 한국동물학회지
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• 제32권3호
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• pp.281-289
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• 1989

포유동물의 생식주기중 대부분의 여포는 폐쇄되고 극히 일부만 배란된다. 돼지의 폐쇄여포액내에는 다량의 androgen이 존재하며 이들이 폐쇄의 한 요인이라는 결과로 보아 폐쇄여포액내에는 다량의 활성적 testosterone이 존재할 것으로 추론하고 이를 정량하고져 하였다. 사람의 여포액내 progesterone, testosterone, estradiol, androstenedion과 dihydrotestosterone의 농도를 본 연구에서 정립된 섬광면역측정법 및 방사면역측정법으로 측정한 결과는 다음과 같았다. 사람의 폐쇄된 여표액내 T,A.DHT의 농도는 소, 중, 대 여포 공히 정상의 여포액내 이들 스테로이드의 농도보다 현저히 증가되어 있었다. 그러나 소여포의 경우 폐쇄여포액내 A의 농도는 정상에 비하여 현저히 낮은 농도를 나타내었다. 반면 P와 E의 폐쇄여포액내 농도는 정상에 비해 현저히 낮은 농도를 나타내었다. 돼지의 소, 중, 대의 폐쇄여포액내 P의 농도는 정상과 통계적으로 유의한 차이가 없었다. T의 경우 역시 각 각의 크기를 가진 폐쇄여포액에서 정상 여포액 농도보다 높게 나타났으나 통계적인 유의성은 없었다. 그러나 E의 경우는 폐쇄여포액내 농도가 정상에 비해 편저히 낮게 나타났다. 한편 여포액내 활성적 T의 점유율은 혈청에 비해 현저히 높았다. 즉 bioavailable T는 정상과 폐쇄여포액내에서 각기 90% 이상을 나타내었다. 위의 결과를 종합해 볼 때 동물중, 크기에 따라 폐쇄의 요인은 다른 것으로 사료되며 폐쇄여포액내 활성적 androgen의 농도는 정상에 비해 높고 또한 E의 농도는 현저히 낮은 것으로 나타났다. 또한 여포액내 대부분의 스테로이드는 활성형의 상태로 존재하며 활성적 T는 폐쇄여포의 판정에 주요기준이 될 수 있을 것으로 판단된다.

• Archives of Pharmacal Research
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• 제25권3호
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• pp.357-363
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• 2002

The effect of a new rhFSH, PG-0801, on oocyte quality, ovulation and in vitro fertilization (IVF) was examined in androgen-sterilized mice. Experimental sterility was induced by a single subcutaneous injection of testosterone propionate (TP, 1 mg/head) into 5 day old female mice. Ovulation was generated in the 10 to 13-week old TP-injected mice by a subcutaneous rhFSH injection (1, 5 or 10 IU/head) followed 48 hours later by a second rhFSH injection (1, 5 or 10 IU/head). For comparison, a subcutaneous PMSG (5 IU/head) injection was used for folliculogenesis and a hCG (5 IU/head) injection was used for ovulation. These were administered using the same protocol. The eggs were harvested from the oviducts and counted 17 to 20 hours after the second injection. IVF was performed by adding sperms ($2{\times}10^{5}/ml{\;}to{\;}2{\times}10^{6}/ml$) to determine the functional activity of the eggs, and the fertilization rate was measured. In addition, the pregnancy rate and fetal development were examined after 15-17 days of gestation. The number of oocytes recovered from the rhFSH/rhFSH group increased dose-dependently and was slightly higher than that of the PMSG/hCG group. The pregnancy rates of the group receiving 1, 5, and 10 IU of rhFSH/rhFSH were 50%, 66.7%, and 75%, respectively, which were significantly higher than that of the control (untreated) group (0%). The numbers of viable fetuses in the 1, 5, and 10 IU/head of the rhFSH/rhFSH group ($8.0{\pm}1.50$, $8.9{\pm}1.02$, and $8.9{\pm}1.12$ fetuses/dam, respectively) were comparable to that of the 5 IU/head PMSG/hCG group ($9.4{\pm}0.94$). The mice receiving rhFSH/rhFSH and PMSG/hCG showed similar fertilization rates (around 65%) via the IVF procedure. These results demonstrate that a new rhFSH, PG-0801, may be useful for inducing ovulation in functionally infertile patients and for superovulation in ovulatory patients participating in assisted reproductive technology (ART) programs.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3337-3343
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• 2013

This systematic review was conducted to assess the efficacy and safety of bisphosphonates for prevention and treatment of osteopenia or osteoporosis in men with non-metastatic prostate cancer receiving androgendeprivation therapy. We searched for randomised controlled trials (RCTs) of bisphosphonates compared with placebo from Pubmed, Embase, the Cochrane Library, and ISI - Science Citation Index. Meta-analyses of prespecified outcomes (bone mineral density, fractures, and adverse events) were performed using Review Manager. Ten RCTs with a total patient population of 1,017 were identified. There was generally more improvement in bone mineral density of the lumbar spine for patients who received bisphosphonate treatment than placebo or other medical treatment at 12 months (WMD 6.02,95%CI 5.39 to 6.65). Similar effects were also observed for total hip, trochanter or femoral neck bone mineral density. However, there was no significant reduction in fractures. Fever and gastrointestinal symptoms were the most common adverse events (10.4% vs. 1.2%; 0.10% vs. 0.03%). Currently, our meta-analysis suggested that oral and intravenous bisphosphonates caused a rapid increase in spine and hip or femoral BMD in non-metastatic prostate cancer patients receiving androgen-deprivation therapy. Fever and gastrointestinal symptoms were common with the use of bisphosphonates. These short-term trials (maximum of 12 months) did not show fracture reduction. In future, more efficient performance of higher quality, more rigorous, large sample, long-term randomised controlled trials (>12 months) are needed where outcomes are detailed.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10729-10733
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• 2015

Background: Adjuvant androgen deprivation therapy (ADT) is a treatment option for prostate cancer (PC) patients after radical prostatectomy (RP). Although it can achieve a good progression-free survival rate, some patients still develop clinical metastasis. We here investigated risk factors of clinical metastasis in post-prostatectomy patients who received immediate adjuvant ADT. Materials and Methods: We identified 197 patients with non-metastatic PC who underwent RP at our institution between 2000 and 2012, followed by adjuvant ADT. The associations of various clinicopathologic factors with clinical metastasis (primary endpoint) and cancer-specific survival (secondary endpoint) were assessed. Multivariate analysis was conducted using a Cox proportional hazards model. Median follow-up was 87 months after RP. Results: Nine (4.6%) patients developed clinical metastasis and six (3.0%) died from PC. Eight of nine metastatic patients had a pathologic Gleason score (GS) 9 and developed bone metastasis, while the remaining one had pathologic GS 7 and developed metastasis only to para-aortic lymph nodes. On multivariate analyses, pathologic GS ${\geq}9$ and regional lymph node metastasis (pN1) were independent predictors of clinical metastasis and pathologic GS ${\geq}9$ was an independent predictor of cancer-specific death. Conclusions: Pathologic GS ${\geq}9$ and pN1 were independent predictors of clinical metastasis in post-prostatectomy patients who received immediate adjuvant ADT. Furthermore, pathologic GS ${\geq}9$ was an indispensable condition for bone metastasis, which may imply that patients with GS ${\leq}8$ on adjuvant ADT are unlikely to develop bone metastasis.

• Annals of Pediatric Endocrinology and Metabolism
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• 제23권4호
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• pp.210-214
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• 2018

Purpose: Premature adrenarche (PA) often leads to polycystic ovary syndrome (PCOS). Higher anti-mullerian hormone (AMH) levels are reported in PCOS. We studied the androgen profile and AMH profiles in Hispanic girls with PA (aged 5-8 years) and age and body mass index (BMI) matched controls. Methods: Retrospective review of electronic medical records of girls who met the inclusion criteria for premature adrenarche were done. Results: PA girls (n=76) were matched to control girls (n=12) for age (mean${\pm}$standard deviation) ($6.7{\pm}1years$ vs. $6.2{\pm}1.3years$) and BMI ($20{\pm}10kg/m^2$ vs. $17.8{\pm}2.7kg/m^2$). Dehydroepiandrostenedione sulfate ($63.3{\pm}51.3{\mu}g/dL$ vs. $29.8{\pm}17.3{\mu}g/dL$, P<0.001) and testosterone levels ($11.4{\pm}4.8ng/dL$ vs. $8.2{\pm}2.9ng/dL$, P=0.001) were significantly higher in the PA group than controls. AMH values (<14 years: reference range, 0.49-3.15 ng/mL) were $3.2{\pm}2.2ng/mL$ vs. $4.6{\pm}3.2ng/mL$ respectively in the PA and control groups and were not different (P=0.4). AMH did not show a correlation with bone age (P=0.1), and testosterone (P=0.9) in the PA group. 17-hydroxyprogesterone levels (17-OHP ng/dL) were $39.5{\pm}30.5ng/dL$ vs. $36.8{\pm}19.8ng/dL$ in PA versus control girls. The concentration of 17-OHP was not statistically different between the control and PA groups. Conclusion: Higher AMH was not observed in PA girls and no correlation with BA and androgen levels was observed.

• 한국식품위생안전성학회지
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• 제38권5호
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• pp.305-310
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• 2023

본 연구는 OECD TG No. 458, 22Rv1/MMTV_GR-KO 전사 활성화 분석법을 포함한 세포 기반 분석법을 사용하여 식품 및 생활용품에 포함된 파라벤과 트리클로산의 인간 안드로겐 수용체를 매개하는 내분비계 교란 가능성을 확인하는 것을 목표로 한다. 4가지 파라벤(메틸-, 에틸-, 프로필-, 부틸-)은 OECD TG No.458에서 AR 길항제로 확인된 반면, 파라벤의 AR 길항 효과는 S9 간 분획물이 있는 경우 나타나지 않았다. 트리클로산 역시 AR 길항제로 분류되었으며, 트리클로산에 의해 유도된 AR 길항 효과는 S9 간 분획물이 존재할 때 제 1상+2상 대사에서 유의하게 감소되었다. 파라벤과 트리클로산에 의해 유도되는 AR 길항 기전은 세포질 내 AR 이량화를 차단하여, 리간드 결합 AR이 핵으로의 전위를 억제함으로써 AR 매개 내분비 교란 효과를 나타냈다. 이러한 결과는 4가지 파라벤과 트리클로산이 AR 이량화 저해를 통한 AR 길항 효과를 나타내는 AR 매개 내분비 교란 가능성을 가지고 있으나, 간 대사 효소가 존재할 경우 내분비 교란 효과는 감소됨을 시사한다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.467-468
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• 2008

• 한국양식학회:학술대회논문집
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• 한국양식학회 2005년도 춘계학술발표대회 논문요약집
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• pp.126-127
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• 2005

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2003년도 춘계 총회 및 학술대회
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• pp.32-32
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• 2003

• 한국독성학회:학술대회논문집
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• 한국독성학회 2005년도 춘계 국제심포지엄 및 학술대회
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• pp.195-195
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• 2005