• Journal of Korean Society of Steel Construction
• /
• v.28 no.3
• /
• pp.129-138
• /
• 2016

Buckling of surface plates is an important limit state in Steel-Plate Concrete (SC) walls under axial compression. The surface plates may be anchored to concrete using connectors of studs or tie-bars. In this paper, the effects of studs and tie-bars on buckling of surface plates were evaluated by conducting tests. Experiments have three types of connectors; all studs, all tie-bars, and the combination of studs and tie-bars. Also, experiments have the various ratios of stud or tie-bar spacing to surface plate thickness. The experimental investigation shows that the buckling shape and strength of the surface plate of SC wall with the combination of studs and tie-bars have good agreements with that of the surface plate of SC walls with all studs or all tie-bars.

• Earthquakes and Structures
• /
• v.23 no.5
• /
• pp.473-491
• /
• 2022

The research investigates experimentally the effect of confinement on structural behavior at the ends of beam-column in reinforced concrete (RC) frames. In the experimental study, five specimens consisting of 1/3-scaled RC frames having single-bay, representing the traditional deficiencies of existing buildings constructed without receiving proper engineering service is investigated. The RC frame specimens were produced to represent most of the existing buildings in Turkey that have damage potential. To decrease the probable damage to the existing buildings exposed to earthquakes, the carbon Textile Reinforced Mortar (TRM) strengthening technique (fully wrapping) was used on the ends of the RC frame elements to increase the energy dissipation and deformation capacity. The specimens were tested under reversed cyclic lateral loading with constant axial loads. They were constructed satisfying the weak column-strong beam condition and consisting of low-strength concrete, such as compressive strength of 15 MPa. The test results were compared and evaluated considering stiffness, strength, energy dissipation capacity, structural damping, ductility, and damage propagation in detail. Comprehensive investigations of these experimental results reveal that the strengthening of a brittle frame with fully-TRM wrapping with non-anchored was effective in increasing the stiffness, ductility, and energy dissipation capacities of RC bare frames. It was also observed that the frame-only-retrofitting with an infill wall is not enough to increase the ductility capacity. In this case, both the frame and infill wall must be retrofitted with TRM composite to increase the stiffness, lateral load carrying, ductility and energy dissipation capacities of RC frames. The presented strengthening method can be an alternative strengthening technique to enhance the seismic performance of existing or moderately damaged RC buildings.

• Journal of the Korean Magnetic Resonance Society
• /
• v.1 no.2
• /
• pp.126-140
• /
• 1997

Mesoporous MCM-41 gallosilicate material was synthesized through shifting through shifting gallosilicate polymer equilibrium towards a MCM-41 phase by addition of acid. The location of Cu(II) exchanged into MCM-41 and its interaction with various adsorbate molecules were investigated by electron spin responance and electron spin echo modulation spectroscopies. It was found that in the fresh hydrated material, Cu(II) is octahedrally coordinated to six water molecules. This species is located in a cylindrical channel and rotates rapidly at room temperature. Evacuation at room temperature removes three of these water molecules, leaving the Cu (II) coordinated to three water molecules and anchored to oxygens in the channel wall. Dehydration at 45$0^{\circ}C$ produces one Cu (II) species located in the inner surface of a channel as evidenced by broadening of its ESR lines by oxygen. Adsorption of polar molecules such as water, methanol and ammonia on dehydrated CuNa-MCM-41 gallosilicate material causes changes in the ESR spectrum of Cu (II), indicating the complex formation with these adsorbates. Cu (II) forms a complex with six molecules of methanol as evidenced by an isotropic room temperature ESR signal and ESEM data like upon water adsorption. Cu(II) also forms a complex containing four molecules of ammonia based on resolved nitrogen superhyperfine interaction.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2005.05a
• /
• pp.143-147
• /
• 2005

The non-pathogenic, non-glycopeptide-producing actinomycete Streptomyces coelicolor carries a cluster of seven genes (vanSRJKHAX) that confers inducible, high-level resistance to vancomycin. The van genes are organised into four transcription units, vanRS, vanJ, vanK and vanHAX, and these transcripts are induced by vancomycin in a vanR-dependent manner. vanHAX are orthologuous to genes found in vancomycin resistant enterococci that encode enzymes predicted to reprogramme peptidoglycan biosynthesis such that cell wall precursors terminate in D-Ala-D-Lac, rather than D-Ala-D-Ala. vanR and vanS encode a two-component signal transduction system that mediates transcriptional induction of the seven van genes. vanJ and vanK are novel genes that have no counterpart in previously characterised vancomycin-resistance clusters from pathogens. VanK is essential for vancomycin resistance in S. coelicolor and it is required for adding Gly branch to stem peptides terminating D-Ala-D-Lac. Because VanK can recognise D-Lac-containing precursors but the constitutively expressed femX enzyme, encoded elsewhere on the chromosome, cannot recognize D-Lac-containing precursors as a substrate, vancomycin-induced expression of VanHAX in a vanK mutant is lethal. Further, femX null mutants are viable in the presence of glycopeptide antibiotics but die in their absence. Bioassay using vanJp-neo fusion reporter system also showed that all identified inducers for van genes expression were glycopeptide antibiotics, but teicoplanin, a membrane-anchored glycopeptide, failed to act as an inducer.

• Journal of the Korean Magnetic Resonance Society
• /
• v.5 no.1
• /
• pp.1-12
• /
• 2001

ZSM-5 gallosilicate molecular sieves was synthesized and cupric ion was ion-exchanged into the gallosilicate. The locations of Cu(ll) species in the framework and their interactions with various adsorbates were characterized by combined electron spin resonance(ESR) and electron spin echo modulation(ESEM) methods. It was found that in a fresh hydrated material, Cu(II) is octahedrally coordinated to six water molecules. This species is located in the channel intersections of two sinusoidal channels and rotates rapidly at room temperature. Evacuation removes some of these water molecules, leaving the Cu(II) coordinated to less water molecules and anchored to of oxygens in the channel wall. Dehydration produces two Cu(II) species, both of which are located in sites inaccessible to oxygen as evidenced by non-broadening of its ESR lines by oxygen. Adsorption of adsorbate molecules such as water, alcohols, ammonia, acetonitrile and ethylene on dehydrated CuNaH-ZSM-5 gallosilicate materials causes changes in the ESR spectrum of Cu(II), indicating the migration of Cu(II) into main channels to form complexes with these adsorbates there. Cu(II) forms a complex with two molecules of methanol, ethanol and propanol, respectively as evidenced by ESR parameters and ESEM data. Cu(II) also forms a square planar complex with four molecules of ammonia, based on the resolved nitrogen superhyperfine interactions and their ESEM parameters. Cu(II) forms a complex with two molecules of acetonitrile based on the ESR parameters and ESEM data. Interestingly, however, only part of Cu(II) interacts indirectly with one molecule of nonpolar ethylene based on ESR and ESEM analyses.

• Journal of the Korean Geotechnical Society
• /
• v.16 no.1
• /
• pp.191-201
• /
• 2000

In this study, the earth retaining structure using a row of piles considering plastic flow of the ground is suggested for shallow excavation works instead of conventional anchored sheet-pile wall method in the marine clays with high groundwater level. The behavior of the earth retaining structure using a row of piles is precisely observed during excavation by inclinometer and piezometer installed in opposite to the excavation side. As a result of field measurement, it was found that the behaviors of the piles and the soil were influenced mainly by slope of excavation face, interval ratio of piles, fixity condition of pile head, and stability number, etc. The earth retaining structure using a row of piles is ascertained for workability, stability, and economical construction on the soft ground having no adjacent structures.

• Journal of Chest Surgery
• /
• v.35 no.11
• /
• pp.822-825
• /
• 2002

The paravalvular leak after redo mitral prosthetic valve replacement is rare but serious complication when it does happen. This condition should be corrected surgically to increase life span and improve symptoms. But simple closure or patch closure of paravalvular leak are not effective in cases of weak annulus or broad defect. We report 3 cases of trido mitral valve replacement using mechanical valve with its sewing ring expanded by a collar of Dacron sheet. The prosthetic sewing ring is anchored on the weak mitral annulus with multiple interrupted sutures, while the Dacron collar is contineuously sutured to the left atrial wall for blood tight sealing. All of the three cases showed event free postoperative course.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.19 no.5
• /
• pp.56-66
• /
• 2015

This study aims at developing a new seismic resistant method by using precast concrete wall panels for existing low-rise, reinforced concrete beam-column buildings such as school buildings. Three quasi-static hysteresis loading tests were performed on one unreinforced beam-column specimen and two reinforced specimens with U-type precast wall panels. Seismic resistant test of anchored and welded steel plate connections manifested an average of 2.8 times increase in the maximum loading (average 591.8 kN) in comparison to unreinforced beam-column specimen. The maximum drift ratios were also shown between 1.4% and 2.7%. An analytical study was performed while assuming the RC column on the right side and the vertical element of the reinforced PC panel to behave in completely composite manner and the RC column on the left side and PC panel to behave in completely non-composite manner when loading was exerted from upper right end of RC frame of specimen to its left side. It was found with the assumptions that the overall flexural behavior in principle agreed with the experimental result.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.4
• /
• pp.515-525
• /
• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• Journal of Ocean Engineering and Technology
• /
• v.17 no.2
• /
• pp.34-39
• /
• 2003

Recent deepwater offshore structures in the Gulf of Mexico utilize butt welded tubular joints. Application of a welded tubular joint includes tendons, production risers, and steel catenary risers. Fatigue life assessment of these joints becomes more critical, as the structures to which they are attached are allowed to undergo cyclic and sometimes large displacements around an anchored position. Estimation of the fatigue behavior of these tubular members in the design stage is generally condrcted by using S-N curves, as specified in the codeds and standards. Applying the stress concentration factor of the welded structure to the S-N approach often results in a very conservative assessment, because the stress field acting on the tubular has a non-uniform distribution through the thickness. Fatigue life analysis using fracture mechanics has been applied in the design of the catenary risers. This technology enables the engineer to establish proper requirements on weld quality and inspection acceptance criteria to assure satisfactory structural integrity during its design life. It also provides guidance on proper design curves and a methodology for accounting for the effects of non-uniform stress distribution through the wall thickness. Still, there is inconsistency when designing tubular joints using a conventional S-N approach and when specifying weld flaw acceptance criteria using fracture mechanics approach. This study developed fatigue curves that are consistent with both the S-N approach and the fracture mechanics approach. Accounting for non-uniform stress distribution and threshold stress intensity factor were key parameters in relating both approaches. A series of S-N curves, generated from the fracture mechanics approach, were compared to the existing S-N curves. For flat plate butt joint, the S-N curve generated from fracture mechanics matches with the IIW class 100 curve when initial crack depth was 0.5 mm (0.02 ). The new curves for tubular joint agree very well with the experimental results. The comparison also indicated the degree of conservatism built into the API X design curve.