• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.2
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• pp.129-134
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• 2001

Since NNK is one of the most abundant tobacco-specific alkaloids and a strong carcinogenic nitrosamine, it has been used for evaluating a potential of carcinogenicity in the animal models. The present study has attempted to examine the potential of carcinogenicity of NNK in human epithelial cells, from which the cell type the most of cancers including oral cancer and nasal cavity cancer are originated. The cellular model used for the study is a human keratinocyte cell system immortalized by Ad12-SV40 hybrid virus. The cellular system has successfully been used for the carcinogenicity studies because of its limitless life span, epithelial morphology and nontumorigenicity. When cells were treated with a variety of NNK concentrations, levels of saturation density and soft agar colony formation were increased in a dose-dependent fashion. Colonies of large cell aggregates were above 5 at the higher doses. The results indicate that exposure of human cells with NNK induced loss of contact inhibition and increases of anchorage independence and cellular adhesion, which are typical characteristics of the neoplatically transformed cells. When cells were exposed with 100uM NNK for 2hr, mRNA levels of IL-1 and PAI-2 were increased in a dose-dependent manner, but expression of TGF- 1 was not affected. While expression of growth regulatory factors were altered with a short-term exposure, there was no alteration of these factors in the NNK-transformed cells. However, mRNA levels of fibronectin were increased both in the short-term treatment and in the transformation. The results suggest that altered expression of extracellular matrix such as fibronectin following short-term exposure might be fixed in the genome and these altered properties be continuously transfered throughout the cell division. Western blot analysis showed a translocation of PKC- from cytosolic fraction to the particulate fraction, indicating a possible role of NNK in the signal transduction pathway. The present study provided an evidence that NNK in the smoking may be associated with epithelial origin cancer such as oral and nasal cavity cancers. In addition, this study suggested that altered expression of extracellular matrix and PKC may play an important role in the carcinogenic mechanism of NNK.

• Korean Journal of Remote Sensing
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• v.29 no.5
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• pp.545-554
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• 2013

Augustine is an active stratovolcano located in southwest of Cook Inlet, about 290 kilometers southwest of Anchorage, Alaska. Between January 11 and 28, 2006, the volcano erupted explosively 14 times. We collected twelve permanent GPS stations operating by Plate Boundary Observatory (PBO) from 2005 to 2011. All data processing was carried out using Bernese GPS Software V5.0 with IGS precise orbit. Static baseline processing by fixing AC59 station was applied for the volcano activity monitoring. AC59 is the nearest (about 24.5 km) station to Augustine volcano, and located on North America Plate including Augustine Island. The test results show inflation (9.7 cm/yr) and deflation (-9.2 cm/yr) of volcano before and after eruption around crater clearly. After volcano activity has reached a plateau, some of the GPS stations installed north of the volcano show ground subsidence phenomenon caused by compaction of pyroclastic flows. These results indicate the possibility of using surface deformation observed by GPS for monitoring and prediction of volcano activity.

• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1308-1321
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• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.

• The korean journal of orthodontics
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• v.39 no.2
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• pp.83-94
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• 2009

Objective: The aim of this study was to investigate the 3-dimensional position of the center of resistance of the 4 maxillary anterior teeth, 6 maxillary anterior teeth, and the full maxillary dentition using 3-dimensional finite element analysis. Methods: Finite element models included the whole upper dentition, periodontal ligament, and alveolar bone. The crowns of the teeth in each group were fixed with buccal and lingual arch wires and lingual splint wires to minimize individual tooth movement and to evenly disperse the forces to the teeth. A force of 100 g or 200 g was applied to the wire beam extended from the incisal edge of the upper central incisor, and displacement of teeth was evaluated. The center of resistance was defined as the point where the applied force induced parallel movement. Results: The results of study showed that the center of resistance of the 4 maxillary anterior teeth group, the 6 maxillary anterior teeth group, and the full maxillary dentition group were at 13.5 mm apical and 12.0 mm posterior, 13.5 mm apical and 14.0 mm posterior, and 11.0 mm apical and 26.5 mm posterior to the incisal edge of the upper central incisor, respectively. Conclusions: It is thought that the results from this finite element models will improve the efficiency of orthodontic treatment.

• The korean journal of orthodontics
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• v.41 no.4
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• pp.297-306
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• 2011

Nonextraction camouflage treatment in mild Class III malocclusion is achieved by backward movement of the lower dentition and forward movement of the upper dentition. Many camouflage treatment modalities have been used for distal tipping and distal movement of mandibular posterior teeth. The amount of distal movement of mandibular dentition can be improved in cases of severe crowding, even without the patient's cooperation, by using miniscrews for anchorage. However, miniscrew insertion may be unsuccessful, and it may contact the adjacent root because of the distal movement of dentition. Distal tipping of mandibular dentition can be achieved using multiloop edgewise archwires and intermaxillary elastics. However, the complexity of this wire design causes discomfort to patients. Recently, a new treatment using improved superelastic NiTi wires (ISWs) and intermaxillary elastics has been introduced. ISWs can deliver orthodontic force more effectively, and their use with molar tip-back treatment has several advantages-this approach is effective, simple, and easy to use and reduces patient discomfort. The aim of this study was to report a case of camouflage treatment using ISW with tip-back and intermaxillary elastics for distal tipping of mandibular posterior dentition and to evaluate the effectiveness of this treatment in a clinical setting.

• Journal of the Korea Concrete Institute
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• v.15 no.6
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• pp.769-777
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• 2003

The strengthening of concrete structures in situ with externally bonded carbon fiber is increasingly being used for repair and rehabilitation of existing structures. Because carbon fiber is attractive for this application due to its good tensile strength, resistances to corrosion, and low weight. Generally bond strength and behavior between concrete and carbon fiber mesh(CFM) is very important, because of enhancing bond of CFM. Therefore if bond strength is sufficient, it will be expect to enhance reinforcement effect. Unless sufficient, expect not to enhance reinforcement effect, because of occuring bond failure between concrete and CFM. In this study, the bond strength and load-displacement response of CFM to the concrete by the direct pull-out test(the tensile-shear test method) were investigated using the experiment and the finite element method analysis with ABAQUS. The key variables of the experiment are the location of clip, number of clips and thickness of cover mortar. The general results indicate that the clip anchorage technique for increasing bond strength with CFM appear to be effective to maintain the good post-failure behavior.

• Journal of Dental Rehabilitation and Applied Science
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• v.27 no.3
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• pp.293-304
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• 2011

The purpose of this study was to investigate the displacement and pattern of stress distribution on periodontal ligaments of maxillary first and second molar, and on orthodontic mini-implant (OMI) surface, according to three different insertion angles to the bone surface of OMI using Dragon helix appliance, which is a newly introduced scissors-bite correcting appliance. OMI were placed between second premolar and first molar with three different insertion angles (45, 60, 90 degrees). Displacement and maximum stress distribution area (MSDA) were analyzed by finite element analysis. When the insertion angle to the alveolar bone surface was 90 degrees, maxillary first and second molar both exhibited MSDA at the palatal root apex. Maxillary first molar did not show any significant displacement, while the second molar exhibited intrusive and palatal displacement. On the OMI, as the insertion angle decreased, the MSDA shifted towards the tip, and the amount of displacement had increased. When the OMI was inserted at a 90 degree angle, anchor loss was minimized and scissors-bite correcting effect was maximized.

• Journal of Dental Rehabilitation and Applied Science
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• v.24 no.2
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• pp.203-211
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• 2008

Minimizing damage to anatomical structure is a prerequisite for skeletal anchorage system to install a miniscrew. This research has focused on evaluating the stability and safety of installation in the maxillary molar buccal area, in which most miniscrews are installed clinically and initial fixation is weak. CT (computerized tomography)images were taken for surveying the possibility of damaging to adjucent teeth in accordance with installation angle. If we install a mini-screw($1.2{\times}6.0mm$) in the maxillary molar buccal area, it would be located generally in the 5~8mm upper of CEJ and 3~5mm inner of the cortical bone surface. We has measured the space between roots And comparison has been made for gender and the space between roots in accordance with the 3 different angles of installation(30 degree, 40 degree, 60 degree) in 3 categories. Category 1 : between 1st molar and 2nd molar Category 2 : between 1st molar and 2nd premolar Category 3 : between 1st premolar and 2nd premolar The result are as follow; 1. The space for category 1 was significantly small. 2. For the installation angle, it was safer to install with steeper angle in category 1 and category 2, but not in category 3. According to these results, the installation a miniscrew in category 2, 3 is safer than in category 1. And it is safer to install with steeper angle in category 1 and category 2.

• Journal of Dental Rehabilitation and Applied Science
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• v.26 no.1
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• pp.47-57
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• 2010

An undisturbed healing process without micromotion at the implant-bone interface is essential for achievement of osseointegration of dental implant. Therefore, initial stability was advocated as prerequisite for successful clinical outcome. Adequate bone quality and quantity were important to achieve initial stability and to prevent early failures. However there were few published data available regarding the effect of design change in implant geometry on initial stability of the implants. The purpose of the current study was to assess the initial stability of various designs of implants when placed into artificial bone materials of varying qualities and shapes of insertion holes. Within the scope of this study, the following results were drawn. Bone quality was major importance to achieve initial stability. Initial stability was higher on GS II which had additional design feature of double thread. With a tapered design of implant such as GS III showed a higher initial stability than straight one. An insertion hole with the similar shape of implant would lead to reduce a compression force on cortical bone and enhance a bone anchorage on cancellous bone.

• Journal of the korean academy of Pediatric Dentistry
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• v.39 no.4
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• pp.404-411
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• 2012

Tooth impaction is defined as a failure of tooth eruption resulting from the physical obstacles in the eruption path or the abnormal position of the tooth germ. Impaction of mandibular second molar is relatively rare, and it may incite pathologic conditions such as dental caries, periodontitis, or root resorption of adjacent first molar. Thus, early diagnosis and treatment is recommended. In the first case, a 10-year-old male patient, was treated by brass wire to separate the bilaterally impacted mandibular second molars. In the second case, a 12-year-old female patient, was treated with Humphrey appliance for impacted mandibular left second molar, which was detected during a periodical dental examination. In the third case, a 17-year-old female patient with impacted mandibular right second molar, was treated by uprighting spring with mini-implant anchorage around premolar area. In the last case, an 18-year-old male patient, was treated with mini-implant placed on the ramus of mandible connected to the orthodontic buttons bonded on impacted mandibular left second molar by elastic thread.