• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.13 no.1
• /
• pp.23-29
• /
• 2014

The purpose of this paper is to assess the structural integrity of piping penetrations for nuclear power plants. A piping qualification analysis describes loads due to deadweight, pressure difference acts normal to the plate, thermal transients, and earthquakes, among other events, on piping penetrations that have been modeled as an anchor. Amodel was analyzed using a commercial finite element program. Apiping penetration analysis model was constructed with an assembly of pipe, head fittings and sleeves. Normally, the design load, thus obtained, will consist of three moments and three forces, referred to a Cartesian coordinate system. When comparing the stress analysis results from each required cutting position, the general membrane stress intensities and local membrane plus bending stress intensities during a structural evaluation cannot exceed the allowable amount of stress for the design loads. Therefore, the piping penetration design satisfies the code requirements.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.5
• /
• pp.770-776
• /
• 2020

Genistein is a type of isoflavonoid found predominantly in leguminous plants. Genistein has diverse biological activities, such as anthelmintic and antioxidant effects, as well as inhibitory effects on the growth of several cancers. In addition, genistein is well known as a phytoestrogen. In this study, we attempted to biologically synthesize genistein from either p-coumaric acid or naringenin using Escherichia coli as a biotransformation host. Four genes, Os4CL, PeCHS, RcIFS, and OsCPR, were used for genistein production. To functionally express RcIFS and OsCPR, two members of the cytochrome P450 family, in E. coli, the membrane-binding anchor domain of each gene was removed, and RcIFS and OsCPR were translationally fused to generate an RcIFS-OsCPR hybrid. Os4CL and PeCHS, or the RcIFS-OsCPR hybrid, were then transformed into E. coli BL21(DE3). Using these strains, we optimized our culture system at a laboratory scale in terms of the cell density, concentrations of substrate and isopropyl-β-D-thiogalactoside, temperature, and culture medium. Under the optimized culture conditions, genistein was produced at up to 35 mg/l and 18.6 mg/l using naringenin and p-coumaric acid, respectively.

• Journal of Korean Society of Steel Construction
• /
• v.12 no.4 s.47
• /
• pp.445-455
• /
• 2000

This study proposed to beam-column joint model consisting of different type structural member to develop new structural system in the structural viewpoint as to a method to overcome various problem according to change of construction environment. This study promoted rigidity and capacity to stiffen reinforced concrete for steel structure end to increase rigidity of long spaned steel beam, and welt to steel flange to anchor U-shaped main bar of SRC structure end to easy stress flow between the different type structure. Through the series of experiments, proposed to possibility of this joint model, and investigated joint rigidity and capacity.

• Journal of Industrial Technology
• /
• v.14
• /
• pp.127-140
• /
• 1994

This study is concerned with the accuracy and the patterns when different methods was used in interpolation task. Although 3 methods employed the same modality for input (visual) and for output (manual responding), they differed in central processing, which method 1 is relatively more tendency of verbal processing, method 2 is realtively more tendency of spatial processing and method 3 needed a number of switching code (verbal/spatial) performing task. Split-plot design was adopted, which whole plot consisted of methods (3), orientations (horizon, vertical), base-line sizes (300, 500, 700 pixels) and split plot consisted of target locations (1-99). The results showed the anchor effect and the range effect. Method 2, method 3 and method 1 that order was better accuracy. ANOVA showed that the accuracy was significantly influenced by the method, the location of target, and its interactions ($method{\times}location$, $size{\times}location$). Analysis of error data, response time and frequency of under, just, over estimate indicated that a systematic error pattern was made in task and methods changed not only the performance but also the pattern. The results provided support for the importance of the multiple resources theory in accounting for S-C-R compatibility and task performance. They are discussed in terms of multiple resources theory and guidelines for system design is suggested by the S-C-R compatibility.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2010.09c
• /
• pp.11-17
• /
• 2010

Numerical analysis was carried out in order to simulate the development of the large deformation that took place on the reinforced earth wall, a part of the Tottori expressway planned to pass Hyogo, Japan. Since this reinforced earth wall had experienced unexpected deformation of the wall during construction, the wall was re-constructed twice. However, the wall deformation showed no sign to cease even at the final stage of the construction. Countermeasures to re-stabilize the wall were demanded. In part I of this paper, it was manifested that subsidence of a 3-meter weak soil due to seepage flow was responsible for the large deformation. A part of concrete panel wall was severely damaged due to extremely large pulling force of geotextile induced by the hammock state. As for the countermeasures, "grouting with slag system" was applied to fill voids of the backfill, and also to prevent further development of settlement in the weak soil layer. "Ground anchor" was also considered to achieve the prescribed factor of safety.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.11 no.1
• /
• pp.1-6
• /
• 1999

Nearshore and offshore pipelines are often applied to carry oil, gas, water and combined products. The thermal and pressure gradients of the fluid inside pipeline cause pipeline expansion. This expansion produces stress to connecting structures with pipeline. Should this stress exceeds the yield strength of connecting components or the allowable displacement of the system, a damage can occur. As most pipelines contain hazardous and toxic fluids, the damage usually leads to fatal accidents involving great economic loss as well. Even subsea pipelines can be easily applied to transport liquid type fluid without time and space constraint, they should be designed and maintained carefully to be functional safely during design lifetime. In this paper, various theories estimating pipeline thermal expansion are investigated and the effects of pipe components to expansion are studied.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.2
• /
• pp.234-238
• /
• 2012

Recombinant Escherichia coli displaying organophosphorus hydrolase (OPH) was used to overcome the diffusion barrier limitation of organophosphorus pesticides. A new anchor system derived from the N-terminal domain of ice-nucleation protein from Pseudomonas syringae InaV (InaV-N) was used to display OPH onto the surface. The designed sequence was cloned in the vector pET-28a(+) and then was expressed in E. coli. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by InaV-N on the outer membrane, and the ability of recombinant E. coli to utilize diazinon as the sole source of energy, without growth inhibition, indicated its significant activity. The location of OPH was detected by comparing the activity of the outer membrane fraction with the inner membrane and cytoplasm fractions. Studies revealed that recombinant E. coli can degrade 50% of 2 mM chlorpyrifos in 2 min. It can be concluded that InaV-N can be used efficiently to display foreign functional protein, and these results highlight the high potential of an engineered bacterium to be used in bioremediation of pesticide-contaminated sources in the environment.

• Geomechanics and Engineering
• /
• v.7 no.2
• /
• pp.201-212
• /
• 2014

Due to the sea-crossing bridge span is generally large and main pier pile foundations are located in deep water and carry large vertical load, sea-crossing bridge main pier pile foundations bearing mechanism and load deformation characteristics are still vague. Authors studied the vertical bearing properties of sea-crossing bridge main pier pile foundations through pilot load tests. Large tonnage load test of Qingdao Bay Bridge main pier pile program is designed by using per-stressed technique to optimize the design of anchor pile reaction beam system. Test results show that the design is feasible and effective. This method can directly test bearing capacity of main pier pile foundations, and analysis bearing behaviors from test results of sensors which embedded in the pile. Through test study the vertical bearing properties of main pier pile foundation and compared with the generally short pile, author summarized the main pier pile foundations vertical bearing capacity and the main problem of design and construction which need to pay attention, and provide a reliable basis and experience for sea-crossing bridge main pier pile foundations design and construction.

• Journal of Korea Multimedia Society
• /
• v.12 no.10
• /
• pp.1436-1449
• /
• 2009

The value of sensing information is decided according to positions of sensor nodes, which are very important in sensor networks. In this paper, we propose a method that estimates positions of nodes by using adjacent node information and received signal strength in a sensor network. With the proposed method, we can find positions of nodes easily because we use information that nodes have. Moreover, we can find distribution easily for all the nodes because we can measure a relative position for a node whose position is not known based on anchor nodes whose positions are already known. We utilized Use case diagram, activity diagram and State machine diagram among several diagrams of UML to implement proposed method in sensor networks that is dynamic system. We can understand exact flow for each function of the proposed method in node position estimation system can be implemented easily. And we can be confirmed that the position of estimated nodes has a little error.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.37B no.11
• /
• pp.984-992
• /
• 2012

In this paper, we discuss a new architecture of future Internet for mobile-oriented environments, named Mobile-Oriented Future Internet (MOFI). The MOFI architecture is designed with Host Identifier and Local Locator (HILL) for identifier and locator separation. Based on the HILL separation architecture, we propose a Dynamic and Distributed Mapping System (DDMS) for identifier-locator mapping control. In DDMS, the mapping control function is distributed onto each access router in the domain, which is different from the centralized approach using a central anchor. For validation of the proposed MOFI-DDMS architecture, we implemented the data delivery and mapping control functions using Linux platform. From the testbed experimental results, we see that the DDMS architecture can give better performance than the existing Proxy Mobile IP (PMIP) protocol in terms of data transmission throughput.