• BMB Reports
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• v.45 no.10
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• pp.589-594
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• 2012

Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated $9.1{\mu}g$ taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and $15.9{\mu}g$ per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.338-340
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• 2021

Morus alba, known as White Mulberry, is one of the most common species of mulberry found in South Korea, along with M. australis, known as Korean Mulberry. During a routine survey to investigate fungal diseases on deciduous broad-leaved trees in 2020, leaf spots were consistently observed on the White Mulberry in Sejong-si (36°30'12.8"N 127°17'34.5"E) and Wonju-si (37°15'29.6"N 128°11'37.9"E), South Korea, with a disease incidence of approximately 70 to 80%. Symptoms included circular, tan or necrotic lesions surrounded by a dark margin on leaves, which, in some cases, the lesions coalesced to form relatively large necrotic regions. The pathogen was successfully isolated from M. alba, and was identified as Cladosporium pseudocladosporioides based on the phylogenetic analysis and morphology. To the best of our knowledge, this is the first report of leaf spot disease on M. alba caused by C. pseudocladosporioides in South Korea.

• Conservation Science in Museum
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• v.10
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• pp.43-61
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• 2009

The Buyeo National Museum was requested conservation treatment for wooden objects excavated from three Baekje archeological sites: Neungsan-ri, Ssangbuk-ri, and Gungnamji Pond. Prior to conservation treatment, analysis was conducted to identify the species used. The results of the analysis revealed wood from diverse species of trees including Hard pine, Cryptomeria japonica D. Don, Zelkova serrata Makino, Quercus spp., Platycarya strobilaceae S. et Z., Castanea spp., Torreya nucifera S. et Z., Taxus cuspidata S. et Z., and Salix spp. A high percentage of the objects were made of Cryptomeria japonica D. Don., a species native to Japan, which indicates that exchange with Japan was active at that time. Among the wooden objects, we analyzed lacquer fragments from six pieces of lacquerware, and the characteristics of the lacquer fragments were peculiar to specific artifacts. Most of the fragments were thicker than 100 ㎛. Pure lacquer and mixed black pigment were used. Infrared spectroscopy of the lacquered wooden fragments revealed that they had a very similar absorption band as refined lacquer, confirming that they were painted with lacquer. For their conservation, we immersed the objects in a high molecular weight aqueous solution of PEG#3,350 (10% → 50%) to strengthen them before vacuum freeze-drying.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.223-230
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• 2007

Vitellaria paradoxa commonly called shea is an important agro forestry and fruit-bearing species present in four agro-ecological zones of Cameroon. The goal of this work was the morphological characterization of certain populations of V. paradoxa which will serve as a necessary step for a subsequent genetic study of the species. Morphological observations related to 20 agronomic traits, studied on 8-13 trees selected from each of the eight shea populations across four different agro-ecological zones located in four provinces of Cameroon were studied. The study showed that there was a variation between the populations, related to the traits measured on the trunk, fruit, nut, and leaf. Three shapes of the tree(ball, broom, and trained), five shapes of the fruit(round, oblong, reversed pear, ovoid, and oblong), three colors of the nut(clear brown, dark brown, and blackish brown) were identified. The principal component analysis(PCA) carried out on the quantitative characters revealed 72% of the total variance expressed on the first and second main axis. This variation was essentially explained by the traits measured on the fruits and on the nuts. The analyses showed that only the traits of the fruits and the nuts were discriminative. The shea populations studied were structured into two distinct groups using these discriminative traits.

• Journal of Korean Society of Forest Science
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• v.82 no.1
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• pp.17-25
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• 1993

Tree-ring data of subalpine conifers growing in Korea were analyzed to evaluate the possibility of enhanced tree growths due to increased atmospheric carbon dioxide. A total of 64 trees of three species(Taxus cuspidata, Pinus koraiensis, Abies koreana) were sampled from three mountain ranges (Seolak, Sobaek, Chiri) at 1200~1750m(above sea level) and four chronologies were developed in spans of 209~412 years. No apparent growth increases were found in the graphs of chronologies. As comparing the growths of 1900-1949 and 1950-1989 period, only one chronology possessed higher mean growth in the post-1950 period. Growth increase found in the Pinus koraiensis chronology from Mt. Seolak was 8% but it could not be solely attributed to increased carbon dioxide concentration.

• Parasites, Hosts and Diseases
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• v.59 no.1
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• pp.89-95
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• 2021

Microcotyle sebastis is a gill monogenean ectoparasite that causes serious problems in the mariculture of the Korean rockfish, Sebastes schlegelii. In this study, we isolated the parasite from fish farms along the coasts of Tongyeong, South Korea in 2016, and characterized its infection, morphology and molecular phylogeny. The prevalence of M. sebastis infection during the study period ranged from 46.7% to 96.7%, and the mean intensity was 2.3 to 31.4 ind./fish, indicating that the fish was constantly exposed to parasitic infections throughout the year. Morphological observations under light and scanning electron microscopes of the M. sebastis isolates in this study showed the typical characteristics of the anterior prohaptor and posterior opisthaptor of monogenean parasites. In phylogenetic trees reconstructed using the nuclear 28S ribosomal RNA gene and the mitochondrial cytochrome c oxidase I gene (cox1), they consistently clustered together with their congeneric species, and showed the closest phylogenetic relationships to M. caudata and M. kasago in the cox1 tree.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.317-327
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• 1993

Stands profiles, yearly changes in growth of annual rings, age and diameter structure, and spatial distribution pattern of individuals in the Pinus densiflora stands around the Yeocheon industrial complex were investigated. Growth of annual ring in Pinus densiflora, which survived when vegetation of this area was damaged by air pollutants, was suppressed for about 10 years since 1974 when factories in this area began to operate, but since then such suppressed growth tended to be recovered. It was supposed that the suppresed growth was originated from air pollution and that improvement of growth since the suppressed period was due to the release from competition with them by death of neighbouring trees and the resuction of the amount of air pollutants. Physiognomy of Pinus densiflora stands showed mosaic pattern composed of different patches. Spatial distribution pattern of individuals an stand profiles were similar to those of Pinus densiflora stands regenerated after natural and artificial disturbances. In an age distribution diagram, age of Pinus densiflora population ranged from 1 to 33 years, Among these individuals were recrited corresponded to the suppresed period of growth of annual ring in Pinus densiflora survived when the vegetation was damaged by air pollution. On the other hand, from the result of analysis of frequency distribution diagram of diameter, it was postulated that even if whis Pinus densiflora community can be maintained as it is for the time being, it might be changed to Quercus community with the lapse of time. Regeneration; Pinus densiflora; Air pollution; Annual ring; Age structure; Diameter structure; Quercus spp. In these analyses, factors for individual recognition and species recognition were suggested.

• Journal of Plant Biology
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• v.25 no.1
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• pp.37-53
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• 1982

Pollen analysis from lake districts, Youngnangho, Wolhamji and Bangeojin, revealed vegetational patterns in Korea. The pollen stratigraphy was divided into five zones, zone L, I, II, IIIa and IIIb for the past 15,000 years. During zone L (earlier than 10,500 yr BP), late-glacial period, Youngnangho was vegetated with a coniferous forest dominated by spruce, larch, haploxylon pine and fir with considerable amount of herbs. Zone I(10,500~7500 yr BP) was predominantly herbaceous vegetationj with significant amount of oak and diploxylon pine. It suggests that the overall environment became milder and drier than late-glacial period. Zone II(7,500~4,000 yr BP), hypsithermal period, showed significant warming condition, indicated by high pollen concentrations of oak, diploxylon pine and hornbeam, and by more diverse flora of deciduous broad-leaved trees than before. Herbs were not an important part of vegetation. Zone III$^a$(4,000~1,500 yr BP) had pine and oak as main elements. Birch increased slightly while hornbeam decreased in this time. It indicates cooling condition. Zone III$^b$(1,5000-present) which can be called pine period showed obvious human interference. Following forest clearance, agriculture was intensified. The beginning of logging and agriculture was discernible by a sudden decline of arboreal species and by considerable amount of rice, buckwheat, sorrel and plantain. Numerous charcoal fragments were observed in zone I and zone III$^b$.

• Mycobiology
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• v.48 no.3
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• pp.184-194
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• 2020

Macrofungi play important roles in forest ecology as wood decayers, symbionts, and pathogens of living trees. For the effective forest management, it is imperative to have a comprehensive overview of macrofungi diversity in specific areas. As a part of the National Institute of Biological Resources projects for discovering indigenous fungi in Korea, we collected macrofungi in Gayasan National Park from 2017 to 2018. These specimens were identified based on morphological characteristics and sequence analysis of internal transcribed spacer (ITS) or the nuclear large subunit rRNA (LSU) region. We discovered 17 macrofungi new to Korea: Butyrea japonica, Ceriporia nanlingensis, Coltricia weii, Coltriciella subglobosa, Crepidotus crocophyllus, Cylindrobasidium laeve, Fulvoderma scaurum, Laetiporus cremeiporus, Lentinellus castoreus, Leucogyrophana mollusca, Marasmius insolitus, Nidularia deformis, Phaeophlebiopsis peniophoroides, Phanerochaete angustocystidiata, Phlebiopsis pilatii, Postia coeruleivirens, and Tengioboletus fujianensis. We described their detailed morphological characteristics.

• Korean Journal of Environment and Ecology
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• v.8 no.2
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• pp.154-159
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• 1995

The Chuwang valley-Kumunkwangi valley forest in Chuwangsan area was studied to investigate forest structure in relation to altitude and part of slope. Forty eight quadrats were set up in the valley forest along altitude of 470m to 780m and part of the slope. Density and basal area of trees in tree strata decreased as increasing elevation. With increasing elevation the importance values of Quercus mongolica, Fraxinus rhynchophylla increased, while those of Pinus densiflora, Lindera obtussiloba decreased. As going from lower part to upper part of the slope, the importance values of Quercus valiabilis and Lindera obtussiloba increased while those of Fraxius rhynchophylla, Acer mono decreased. Species diversity tended to decreased as going to upper parts of the slope. The range of similarity indices between elevation belts, and parts of the slope were 74.4~84.2% and 68.0~96.3%, respectively. According to importance value and cluster analysis, the studied valley forest was classified into three forest communities of Pinus densiflora-deciduous tree species community of lower part of slope, Pinus densiflora-Quercus variabilis community of middle and upper part of slope, Pinus densiflora community of the top area.