• 제목/요약/키워드: Amyda sinensis

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자라 위장관 내분비세포에 관한 면역조직화학적 연구 (An immunohistochemical study on the gastro-entero-endocrine cells of the pond tortoise (Amyda sinensis))

  • 김종범;이재현;이형식;이남수
    • 대한수의학회지
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    • 제30권4호
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    • pp.383-394
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    • 1990

  • The gastrointestinal endocrine cells of the Pond tortoise, Amyda sinensis were studied immunohistochemically, and somatostatin-, gastrin/cholecystokinin(GAS/CCK)-, glucagon-, 5-hydroxytryptamine(5-HT)-, insulin- and chromogranin-immunoreactive cells were revealed. The characteristic findings of the regional distribution and relative frequency of these immunoreative cells in the gastrointestinal tract of the Pond tortoise were as follows; A few somatostatin-immunoreactive cells were distributed from the segment I to the segment V. GAS/CCK-immunoreactive cells were found from the segment III to the segment VII. These cells were numerous in the segment III and a few in the other segments. A few glucagon-immunoreactive cells were found in the segment I and rare in the segment II. 5-HT-immunoreactive cells were found throughout the gastrointestinal tract. Numerous numbers of them were found in the segment I, while moderate or a few in the other segments. Insulin-immunoreactive cells were distributed from the segment II to the segment IX. Moderate numbers of them were found in the segment VIII and IX, while a few in the other segments. Chromogranin-immunoreactive cells were found from the segment III to the segment VI. Moderate numbers of these cells were found in the segment IV and V, while a few in the other segments. BPP-immunoreactive cells were not observed throughout the gastrointestinal tract of the Pond tortoise, Amyda sinensis.

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韓國産 자라(Amyda sinensis)의 脾臟組織에 關하여 (Histological Studies on the Spleen of Mud Turtle (Amyda sinensis))

  • 정호삼;이경로
    • 한국동물학회지
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    • 제18권3호
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    • pp.127-130
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    • 1975

  • 1) 자라의 비장을 싸고있는 皮膜은 筋內細胞가 없으며 哺乳類의 그것과 마찬가지로 망상섬유와 탄력섬유 및 교원섬유를 함유하고 있다. 2) 자라의 비장의 脾株는 개구리 비장보다 발달되고 짧은 脾株가 잘 발달되어 있다. 3) 자라 비장의 血管系는 哺乳類와 거의 비슷하나 특히 中心動脈이 잘 발달되고 脾洞의 발달은 포유류의 脾洞構造나 數量面에서 下等性을 보인다. 4) 자라 비장의 造血細胞의 출현빈도가 포유류와 양서류등 다른 脊椎動物보다 훨씬 많이 出現된다.

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별갑이 아토피 피부염에서의 알러지성 염증 반응에 미치는 영향 (Effects of Amyda sinensis on Allergic Inflammation Mechanism related Atopy Dermatitis)

  • 심태경;고대경;김현창;백연종;이재석;유화승
    • 혜화의학회지
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    • 제20권1호
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    • pp.69-83
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    • 2011

  • Objectives: This study aimed to investigate the effects of Amyda sinensis (AS) on allergic inflammation mechanism related atopy dermatitis. Methods: To investigate the effects of AS, We study inhibitory effect of AS on the levels of pro-inflammatory cytokines released from Raw264.7 cell stimulated with LPS (lipopolysaccaride), and EoL-1, THP-1, Jutkat cell stimulated with DP (Dermatophagoides pteronyssinus), and LPS indused acute inflammatory BALB/c mouse model. Result: AS reduced the levels of IL-$1{\beta}$ released from Raw264.7 cell stimulated with LPS at 20 ug/ml, 5 ug/ml concentration, and reduced the levels of IL-6 in a dose-dependent. AS significantly reduced the levels of MCP-1 released from EoL-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at all the concentration, and significantly reduced the level of IL-8 at 0.1 ug/ml concentration. AS significantly reduced the levels of MCP-1 released from THP-1 cell stimulated with DP (Dermatophagoides pteronyssinus) at 1 ug/ml concentration, and reduced the level of IL-6 in a dose-dependent. AS significantly reduced the levels of IL-4 released from Jutkat cell stimulated with DP at all the concentration, and significantly reduced the level of IL-5 at 0.1 ug/ml. 1 ug/ml concentration,. and reduced the level of TNF-${\alpha}$ in a dose-dependent. AS significantly reduced the levels of TNF-${\alpha}$, IL-6, IL-$1{\beta}$, in LPS indused acute inflammatory BALB/c mouse model, in a dose-dependent. Conclusion: These result suggested that AS has suppressive effect on pro-inflammatory cytokines in various cell lines through the regulation of immune system. AS could be applied on the medicinal sources for treatment of immune abnormal diseases such as atopy dermatitis afterward.

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자라 위 및 십이지장 내분비세포에 관한 전자현미경적 연구 (Electron Microscopic Study on the Endocrine Cells in the Stomach and Duodenum of the Pond Tortoise(Amyda sinensis))

  • 전창진;이재현;이창헌
    • Applied Microscopy
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    • 제16권2호
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    • pp.25-34
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    • 1986

  • 자라의 위 및 십이지장에 출현하는 내분비세포의 형태를 알아보기 위하여 전자현미경으로 관찰을 행하였던 바, 과립의 크기, 형태, 전자밀도 및 세포의 형태 등에 의해 다음과 같이 6종의 세포형으로 구분할 수 있었다. I형 : 직경 $100{\sim}430nm$(평균 290nm)로서 전자밀도가 낮고 많은 수의 다양한 형태의 과립을 가진 것. II형 : 직경 $190{\sim}430nm$(평균 280nm)로서 높은 전자밀도를 나타내며 다양한 형태의 과립을 가진 것, III형 : 직경 $170{\sim}650nm$(평균 430nm)로서 전자밀도가 높고 과립막과 내용물 사이에 넓은 halo를 형성하는 과립을 가진 것. IV형 : 직경 $140{\sim}370nm$(평균 240nm)로서 전자밀도가 대체로 낮고 과립막과 내용물이 밀착된 과립을 가진 깃. V형 : 직경 $300{\sim}600nm$(평균 410nm)로서 전자밀도는 낮거나 중등도이며, 과립은 곽립막과 내용물 사이에 명확한 halo를 형성하고, 세포질내 미세섬유를 가지는 것. VI형 : 직경 $100{\sim}220nm$(평균 160nm)로서 대체로 소형의 과립을 가지며, 전자밀도는 다양하고 과립은 과립막과 내용물 사이에 halo를 형성하는 것, 밀착된 것 또는 내용물이 결정구조를 나타내는 것 등으로 다양하며 세포질내 풍부한 미세섬유를 가지는 것.

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