• Title/Summary/Keyword: Ammonium sulfate

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Properties of Crude Amylase Isolated from Pine Nut (잣에 존재하는 아밀라제의 특성)

  • Kim, Jong-Sang;Seog, Ho-Moon
    • Korean Journal of Food Science and Technology
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    • v.26 no.4
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    • pp.398-402
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    • 1994
  • The participation of thermostable amylase in the decrease of viscosity of pine nut's porridge was investigated using the crude enzyme obtained from ammonium sulfate fractionation of pine nut extracts. The fraction precipitated at $35{\sim}55%$ saturation of ammonium sulfate had the highest specific activity of the enzyme. ${\alpha}-amylase$ activity was maximal at $75^{\circ}C$, pH 5.4. Amylograph data showed that addition of the enzyme to rice flour resulted in the significant decrease of its viscosity, suggesting the existence of thermostable ${\alpha}-amylase$ in pine nut.

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Effect of Sterilizing Method on the Quality Change of Iron Fortified Market Milk during Storage (살균방법이 철분강화 우유의 저장중 품질변화에 미치는 영향)

  • 김윤지;김기성
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.4
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    • pp.755-759
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    • 1999
  • To evaluate the effect of sterilizing method on the quality of iron fortified market milk, HTST(high temperature, short time) or LTLT(low temperture, long time) method was adopted after addition of 100ppm ferrous sulfate, ferric citrate, ferric ammonium citrate, or ferrous lactate in market milk. Sterilized iron fortified market milk was stored at 4oC and then pH, lipid oxidation, color change, and sensory quality were observed. The range of pH change in iron fortified market milk sterilized by HTST or LTLT was 6.51~6.74. The order of pH was control>ferric ammonium citrate>ferrous lactate>ferrous sulfate>ferric citrate. Oxygen consumption of ferric ammonium citrate and ferric citrate was lower than ferrous lactate and ferrous sulfate. This trend was same in HTST and LTLT method, but generally oxygen consumption was lower in iron fortified market milk sterilized by LTLT method than by HTST. In total color change, ferrous lactate treatment was closer to control than other treatments. Also sensory characteristics of ferrous lactate treatment was showed better quality than other treatment. From these results, LTLT method was more suitable than HTST method for iron fortified market milk and ferrous lactate was comparably suitable among iron salts used in this study.

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Size Distribution of Ambient Aerosol Measured at a Coastal Site in Jeju Island (제주도 해안가에서 측정된 에어로졸의 성분별 입경분포 특성)

  • 이기호;양희준;허철구
    • Journal of Environmental Science International
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    • v.12 no.10
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    • pp.1043-1054
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    • 2003
  • During the period from April to September 2002, the size distributions of ambient aerosol were measured at the coastal site at Hamduk in Jeju Island. Na$\^$+/, K$\^$+/, Mg$\^$2+/, Ca$\^$2+/and Cl$\^$-/ exhibited mostly a bimodal coarse mode size distribution, while ammonium and sulfate were mainly in the fine size range, with maximum at around 0.54$\mu\textrm{m}$. The average molar concentration ratio of ammonium to sulfate for fine particles was equal to 2.0${\pm}$0.9. Nitrate was evenly found in both the coarse and fine modes. Elements like Al, Fe, Cu, Mg, Na, Ti, Sr and Mn were dominant in coarse particles, with the maximum at around 5.25$\mu\textrm{m}$. S and Pb were mainly in the submicrometer size range. Other elements with a fine and coarse modes were V, Ni, Cu, Ba and Mo. The patterns of the size distributions of trace elements measured at the downtown in Jeju City were very similar to those at the coastal site in Hamduk. However, the amplitude of size fractional concentrations at Jeju City was narrower than that at Hamduk. While the mass median diameters for the chemical species originated from the natural origin such as marine and crust were relatively large, those for ammonium, sulfate, S and Pb were very small.

Electrodeposition of Manganese from Ferromanganese Slag with Ammonium Sulfate (황산암모늄에 의한 훼로망간 슬랙으로부터 망간의 전착)

  • Duk Mook Kim
    • Journal of the Korean Chemical Society
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    • v.29 no.4
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    • pp.390-396
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    • 1985
  • The manganese was extracted from ferromanganese slag with 6N ammonium sulfate and purified with ammonium sulfide. The current efficiencies were substantially increased when small amounts of selenious acid is used as an additive. Stainless steel was used as cathodic electrode and lead (+1% Ag) as anodic electrode. The effects of several variables were investigated, and the optimum conditions were found to be; 40g/l Mn in electrolyte at pH 7.0 with 1$20g/l (NH_4)_2SO_4$, Cathode current density 60mA/cm$^2$, Current efficiency 90% and up at the temperature about $25^{\circ}C.$ The metal produced has been consistantly of high quality.

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Ergothioneine Contents of Shiitake (Lentinula edodes) Fruiting Bodies on Sawdust Media with Different Nitrogen Sources

  • Jang, Yeongseon;Park, Jiheon;Ryoo, Rhim;Park, Youngae;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.44 no.2
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    • pp.100-102
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    • 2016
  • Ergothioneine is a natural compound with strong antioxidant properties. In this study, the effects of different nitrogen sources including ammonium nitrate, ammonium sulfate, sodium nitrate, and histidine in sawdust media were investigated to enhance ergothioneine contents in Shiitake (Lentinula edodes) fruit bodies. The addition of 0.2% ammonium sulfate in the growth media showed the highest enhancement of ergothioneine content in shiitake fruit bodies which was 1.7-fold higher than the control. On the other hand, histidine, a building block of ergothioneine decreased the concentration of ergothioneine significantly. Our results demonstrate that the cultivation of shiitake in sawdust media with suitable nutrients was effective to enhance its ergothioneine contents.

A Study on the Characteristics of Ammonium Thiocyanate in Fricke Dosimeter Solution (Fricke선량계용액중(線量計溶液中) Ammonium Thiocyanate의 특성(特性)에 대(對)한 연구(硏究))

  • Kim, Chang-Kyun;Huh, Joon
    • Journal of radiological science and technology
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    • v.2 no.1
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    • pp.89-93
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    • 1979
  • Fricke dosimeter solution in chemical dosimeters consists of Ferrous sulfate, Sodium chloride and Sulfuric acid. Authors made a new experiment on the characteristics of dosimeter solution with Ammonium Thiocyanate and the results obtained were as follows; 1. The chemical oxidation response after irradiation was very rapid(within fifteen minutes). 2. A preventive measure of the spontaneous oxidation as time goes on was possible to use 0.1 mM instead of 1mM Ferrous sulfate solution. 3. 477nm in wavelength of spectrophotometer was most ideal to measure optical density in this solution

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Improved Procedure for Purification of Clostridium botulinum type B Toxin (Clostridium botulinum Type B 독소의 정제방법에 관한 연구)

  • 박문국;양규환
    • Korean Journal of Microbiology
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    • v.20 no.4
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    • pp.183-188
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    • 1982
  • The neurotoxin of Clostridium botulinum type B was purified from a liquid culture. The purification steps consist of ammonium sulfate precipitation of whole culture, treatment of Polymin P(0.15%, v/v), gel filtration on Sephadex G-100 at pH5.6 and DEAE-Sephadex charomatography at pH8.0. The procedure recovered 17% of the toxin assayed in the starting culture. The toxin was homogeneous by sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis and had a molecular weight of 163, 000. Subunits of 106, 000 and 56, 000 molecular weight were found when purified toxin was treated with a disulfide-reducing agent and electro phoresed on SDS-polyacrylamide gels.

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Preparation of $Mn_{0.5}Zn_{0.5}$ Ferrite Powder by Coprecipition Process (공침법에 의한 $Mn_{0.5}Zn_{0.5}$ Ferrite 분말제조연구)

  • 엄태형;서동수
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1991.10a
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    • pp.33-36
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    • 1991
  • For its outstanding magnetic property, preparations of MnZn ferrite were studied with various method. In this study, MnZn ferrite powders were prepared from ammonium oxalate and mixed metal sulfate by the controlled coprecipitation process. Considering to low dissolved each metal ion, high production yield and particle size, the established optimum reaction conditon by the statistical analysis of each results are that reaction temperature is $25^{\circ}C$, concentration of metal sulfate is 0.3M, molar ratio of ammonium oxalate/metal sulfate is 1.1:1. The effective experimental factor and characterization of the precipitated powder at optimum condition were studied.

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

  • Jeon, Jong-Min;Thangamani, Rajesh;Song, Eunjung;Lee, Hyuk-Won;Lee, Hong-Weon;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.211-217
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    • 2013
  • Corynebacterium glutamicum is one of the well-studied industrial strain that is used for the production of nucleotides and amino acids. Recently, it has also been studied as a possible producer of organic acids such as succinic acid, based on its ability to produce organic acids under an oxygen deprivation condition. In this study, we conducted the optimization of medium components for improved succinate production from C. glutamicum under an oxygen deprivation condition by Plackett-Burman design and applied a response surface methodology. A Plackett-Burman design for ten factors such as glucose, ammonium sulfate, magnesium sulfate, potassium phosphate ($K_2HPO_4$ and $KH_2PO_4$), iron sulfate, manganese sulfate, biotin, thiamine, and sodium bicarbonate was applied to evaluate the effects on succinate production. Glucose, ammonium sulfate, magnesium sulfate, and dipotassium phosphate were found to have significant influence on succinate production, and the optimal concentrations of these four factors were sequentially investigated by the response surface methodology using a Box-Behnken design. The optimal medium components obtained for achieving maximum concentration of succinic acid were as follows: glucose 10 g/l, magnesium sulfate 0.5 g/l, dipotassium phosphate ($K_2HPO_4$) 0.75 g/l, potassium dihydrogen phosphate ($KH_2PO_4$) 0.5 g/l, iron sulfate 6 mg/l, manganese sulfate 4.2 mg/l, biotin 0.2 mg/l, thiamine 0.2 mg/l, and sodium bicarbonate 100 mM. The parameters that differed from a normal BT medium were glucose changed from 40 g/l to 10 g/l, dipotassium phosphate ($K_2HPO_4$) 0.5 g/l changed to 0.75 g/l, and ammonium sulfate ($(NH_4)_2SO_4$) 7 g/l changed to 0 g/l. Under these conditions, the final succinic acid concentration was 16.3 mM, which is about 1.46 fold higher than the original medium (11.1 mM) at 24 h. This work showed the improvement of succinate production by a simple change of media components deduced from sequential optimization.

Comparison of Yield and Content of Salidroside with Application Rates of Nitrogenic Fertilizer under Forcing Culture of Rhodiola rosea L. (바위돌꽃(Rhodiola rosea L.)의 촉성재배시 질소 시비에 따른 수량과 Salidroside 함량)

  • Lee, Kang-Soo;Choi, Sun-Yeong;Li, Long-Gen;Hwang, Seon-Ah
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.124-130
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    • 2008
  • This experiment was conducted to investigate the optimum nitrogen fertilization conditions for the production of high-quality Rhodiola rosea L. in forcing culture. Up until 48 kg N/10 a of both urea and ammonium sulfate, dry matter yield of root of Rhodiola rosea L. tended to increase with increase in application rates, however, it decreased thereafter in higher application rates. In the case of urea, the content of salidroside in the root of the Rhodiola rosea L. appeared to decrease rapidly from the application rates of 64 kg N/10 a and over. Meanwhile, the content of salidroside in the root tended to decrease gradually with the application rates exceeding 64 kg N/10 a of ammonium sulfate. The optimum fertilization rates of urea and ammonium sulfate was 45-8-20-10-10 (N-P-K-Ca-Mg) kg/10 a according to the curvilinear regression equation. However, considering the nitrogen accumulation in soil, nitrogen translocation into the plant, and dry matter yield and content of salidroside in the root of Rhodiola rosea L., the optimum fertilization rates of urea and ammonium sulfate would be 40-8-20-10-10 kg/10 a and 35-8-20-10-10 kg/10 a, respectively.