• Title/Summary/Keyword: Amino Nitrogen

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Effect of Bacillus Strains on the Chungkookjang Processing -III. Changes of the Free Amino Acid Contents and Nitrogen Compounds during Chungkookjang Koji Preparation- (균주(菌株)를 달리한 청국장 제조(製造)에 관(關)한 연구(硏究) -제3보(第三報) : 청국장의 유리(遊離) 아미노산(酸) 함량(含量)과 질소성분(窒素成分)-)

  • Suh, Jeong-Sook;Ryu, Myung-Ki;Hur, Yun-Hang
    • Korean Journal of Food Science and Technology
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    • v.15 no.4
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    • pp.385-391
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    • 1983
  • The change of free amino acid contents and nitrogen compounds in the course of the Chungkookjang fermentation that occurred by utilizing Bacillus natto and Bacillus subtilis are to the following effects. pH, during the growth period, that is 6.35 in pH at the first stage of fermentation, were turned into 8.2 after 72 hours. Crude protein content increased irregularly from 16.82%-18% and total sugar decreased. Increasing with the progress of fermentation time, protease activity showed the maximum value between 48-60 hours, but Bacillus natto activated a little than Bacillus subtilis. Amino nitrogen and water soluble nitrogen content increased but difference was found that is, Bacillus natto increased more than Bacillus subtilis. Glutamic acid content was the highest among the contents of free amino acid between both Bacillus sp. and the order of the next contents showed as leucine, phenylalanine, histidine alanine. arginine, but difference was found between Bacillus sp., that is, Bacillus natto was higher than Bacillus subtilis. In view of the results as above, Bacillus natto was excellent than Bacillus subtillus as Bacillus strains of Chungkookjang koji production.

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Physicochemical characteristics of radish kimchi supplemented with seafood (gizzard shad) during fermentation (뼈째 포함한 전어 무섞박지 김치의 발효 중 이화학적 품질 특성 연구)

  • Park, Inmyoung;Song, HoSu;Cho, Seong Soon
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.601-607
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    • 2018
  • We examined the physicochemical characteristics of radish kimchi supplemented with either 10% boneless or boned sliced gizzard shad (BLGS and BGS, respectively) and compared them to those of untreated kimchi during an eight-week fermentation period. BLGS- and BGS-containing kimchi showed higher acidity, amino nitrogen, and Lactobacillus levels than control kimchi. Furthermore, BGS-containing kimchi showed higher acidity, amino nitrogen, and Lactobacillus levels, and lower reducing sugar contents than BLGS at the optimum- and over-ripened stage. Higher amino nitrogen serves as a good medium for microorganism growth, which produce organic acids, consequently decreasing the reducing sugar, pH levels and increasing the acidity. The preference test showed that the control kimchi was the most preferred at the unripened stage, while the BLGS- and BGS-supplemented kimchi samples in the ripened and over-ripened stages showed similar preferences. In conclusion, our results indicate that gizzard shad-supplemented kimchi shows positive health characteristics, such as low salinity, high amino nitrogen and Lactobacillus levels.

Effect of Garlic on Quality of Low Salted Anchovy - 2. Changes of Nitrogenous Compounds - (마늘 첨가가 저염 멸치젓의 품질에 미치는 영향 - 제2보 함질소화합물의 변화 -)

  • 진양호;권오천;성낙주;신정혜;강민정
    • Culinary science and hospitality research
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    • v.8 no.3
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    • pp.279-294
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    • 2002
  • The anchovy, Engraulis japonica, were prepared with two different salt concentration of 20%, 10% which was added 2, 5, 5 and 10 % of grind garlic(LSA 1, 2, 3, 4) and garlic juice(LSB 1, 2, 3, 4), respectively. The experimental samples were taken at 30, 60, 90 and 110 days fermentation, which were analyzed VBN(volatile basic nitrogen), amino acids, nucleotides and their related compounds and amino acids. VBN increased rapidly until 110 days fermentation, its contents in samples containing of 10% salt(CB) were increased about 2.4 times as compared with those of 30 days fermentation. And then its contents of 110 days fermented samples increased at average 2.3 times more than 30 days fermented samples containing grind garlic of 2, 5, 8% and garlic juice of 2%. Nucleotide and their related compounds were detected AMP, UMP, IMP, inosine and hypoxanthine. Hypoxanthine was dominants in all samples and increased steadily during fermentation of anchovy. The contents of composition amino acids decreased gradually during it's fermentation, but those content decreased 33% in CO group, 42% in the CB group and 38%(average) in the other garlic added low salt groups.

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Quantitative Comparison of Ginsenosides and Nitrogen Compounds in Korean Ginsengs and Related Origin (고려인삼류와 유사생약중 ginsenoside 및 질소화합물의 함량 비교)

  • Shin, Jae-Young;Park, Hee-Juhn;Lim, Sang-Cheol;Jung, Won-Tae
    • Korean Journal of Pharmacognosy
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    • v.27 no.1
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    • pp.6-14
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    • 1996
  • To differentiate the quality of Korean ginseng from those of other habitats, the quantitative analysis of free amino acids(FAA) and total amino acids(TAA) in addition to ginsenoside Rb1 and Rg1 was carried out using amino acid analyzer and HPLC, respectively. FAA pattern in Korean ginseng was much different from that of Panax notoginseng. The difference in total content of FAA was also found that Korean ginseng contained 26.3-39.8mg/g while Panax notoginseng contained 6.5mg/g. This FAA content had a tendency to increase with the age of radix. The contents of FAA and TAA in Korean ginseng(6 years old) from Kumsan were shown to be the highest than other ginseng origins tested. The content in the 6 years Panax ginseng from China was about same with that of 4 years Korean ginseng of Kumsan. However, regarding to gisenoside Rbl and Rg1, which have been accepted as the characteristic components of Panax ginseng-Panax notoginseng showed considerably higher content than those of any other ginseng origin.

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Physico-chemical Properties of Fermented Soybean Roasted on Different Roasting Conditions (볶음 조건에 따른 발효 콩의 이화학적 특성 연구)

  • You, Min jung;Choi, Nam-Soon
    • Journal of the Korean Society of Food Culture
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    • v.31 no.6
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    • pp.643-651
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    • 2016
  • This study was investigated the difference in the quality characteristics of the fermented soybean roasted with different conditions for making chungyukjang. Roasted condition of soybean was decided by pre-test, which was on $140^{\circ}C$ for 21 min, $180^{\circ}C$ 9.5 min, and $220^{\circ}C$ 6 min. Fermented soybean with different roasted condition and conventionally made cheonggukjang were measured for proximate composition, color, pH, amino nitrogen, ammonia nitrogen, free sugar and amino acids. The lightness and yellowness of soybean fermented by conventional method was higher than those of the fermented soybean with roasted soybean. The contents of free sugar of fermented soybean roasted on $220^{\circ}C$ for 6 min (FS220) was the highest among the group and followed by fermented soybean roasted on $180^{\circ}C$ for 9.5 min (FS180), $140^{\circ}C$ 21 min (FS140) and conventional cheonggukjang (FS0). The contents of total free amino acid was highest on fermented soybean roasted on $140^{\circ}C$ for 21 min (FS140) and followed by fermented soybean roasted on $180^{\circ}C$ for 9.5 min (FS180), $220^{\circ}C$ 6 min (FS220) and conventional cheonggukjang (FS0).

Processing and Quality Characteristics of Rapidly Fermented Anchovy Engraulis japonicus Sauce with Radish Raphanus sativus L. (무(Raphanus sativus L.) 첨가 속성발효 멸치(Engraulis japonicus) 액젓의 제조 및 품질)

  • Oh, Kwang-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.4
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    • pp.417-424
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    • 2022
  • To develop a value-added anchovy Engraulis japonicus sauce, the processing conditions and quality characteristics of a rapidly fermented and high-purity anchovy sauce (RRAS) were examined by adding 5-10% (w/w) radish Raphanus sativus L. RRAS exhibited higher yield, total nitrogen content, and amino nitrogen and calcium contents as well as lower salinity than those of conventional anchovy sauce (control). The salinity, yield, and total amino acid contents of RRAS and control were 17.2-17.7% and 19.6%, 81.2-88.7% and 61.0%, and 13,117.8-14,174.9 mg/100 g and 10,041.1 mg/100 g, respectively. The major amino acids recorded were aspartic acid, glutamic acid, alanine, valine, isoleucine, leucine, phenylalanine, ornithine, lysine, and histidine. The histamine contents of RRAS and control were 8.3-8.6 mg/100 g and 19.2 mg/100 g, respectively, while the protease activity levels were 0.901-0.958 unit/mg and 0.695 unit/mg, respectively. Overall, this study establishes that RRAS can not only significantly shorten the salt fermentation period, but can also serve as an anchovy sauce with superior nutritional quality and higher levels of amino acid and calcium.

The Effect on the Protein Metabolism in Albino Rats by feeding on the Rice Mixed with Wheat or Barley Diet (미맥혼식(米麥混食)이 백서(白鼠)의 단백질(蛋白質) 대사(代謝)에 미치는 영향(影響))

  • Park, Sae-Yul
    • Journal of Nutrition and Health
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    • v.6 no.3
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    • pp.9-15
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    • 1973
  • Some effect on the protein metabolism in growing albino rats by feeding on the rice mixed with wheat or barley have been studied. The species of wheat and barley used in this experiment were either 80% polished or nonpolished wheat, barley and naked barley. The growing rats to be examined were fed on 30% wheat or barley mixed with rice diets for 8 weeks. The total nitrogen, creatinine, amino acid nitrogen and urea-nitrogen contents in the liver and the creatinine and urea-nitrogen contents in the urine have been measured. The results obtained are summarized as follows: 1. The total nitrogen contents in the liver and the serum were no remarkable difference by feeding on each mixed diet, compared with the rice diet group. 2. The creatinine contents in the liver of the unpolished wheat and barley mixed diet groups were the similar to that of the rice diet group, but these were higher by feeding on the polished wheat and barley mixed with rice diets. 3. The amino acid nitrogen contents in the liver of the polished naked barley mixed with rice diet groups were the similar to that of the rice diet group, but these were higher by feeding on the other mixed diets than the rice diet. 4. The urea-nitrogen contents in the serum of the polished wheat and naked barley mixed with rice diet groups were higher than that of the rice diet group, but these were significantly lower by feeding on the polished barley mixed with rice diets than the others. 5. The creatinine and the urea-nitrogen contents in the urine of the original wheat and barley mixed with rice diet groups were higher than that of the polished wheat and barley mixed with rice diet groups. In the view of the above results, it could be seen that the protein metabolism was remarkable change according to polish of the wheat and barley.

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Photoreaction of 2'-Halobenzanilide: Synthesis of 2-Phenylbenzoxazole

  • Ahmed M. Mayouf;Park, Yong-Tae
    • Journal of Photoscience
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    • v.7 no.1
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    • pp.5-8
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    • 2000
  • Irradiation of 2'-bromobenzanilide in acetonitrile containing sodium hydroxide under nitrogen leads to the formation of the intramolecular photosubstituted product, 2-phenylbenzoxazole (45 %) along the minor photoreduced and photo-Fries type product. The photoreaction of 2'chlorobenzanilide under the same condition as above gives the photo-Fries type reaction product, 2-amino-3-chlorobenzophenone (22%) with minor product, 2-phenylbenzoxazole. The photoreaction of 2'-chlorobenzanilide in acetonitrile produces a photocyclized product, phenanthridone (19%) along with minor products, 2-phenylbenzoxazole, benzanilide, 2-amino-3-chlorobenzophenone, and 4-amino-3-chlorobenzophenone, while that of 2'-bromobenzanilide produces photosubstituted product, 2- phenylbenzoxazole.

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Effects of Nitrogen and Sulfur Application on Yield and contents of Amino Acids Containing Sulfur of Rice (질소(窒素) 및 유황시용(硫黃施用)이 수도(水稻)의 수량(收量)과 함유황(含硫黃)아미노산(酸) 조성(組成)에 미치는 영향(影響))

  • Kim, Bok-Jin;Back, Jun-Ho;Choi, Hyuk
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.122-128
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    • 1997
  • A pot experiment was carried out using Seomjin-byeo and Samgang-byeo to investigate the effects of nitrogen and sulfur on the growth, yields, and sulfur containing amino acid content of rice. The yields and number of tillers at 50 days after transplanting were increased with the increase of the amounts of nitrogen and sulfur, but N/S ratio was decreased in both varieties. There were a positive correlations between yield and contents of nitrogen and sulfur in straw and brown rice, but a negative correlation between N/S ratio and yields at harvesting stage. Crude protein contents increased with the increase of amounts of nitrogen and sulfur. contents of amino acid containing sulfur in brown rice were positively correlated with the sulfur and nitrogen contents in rice straw.

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Dietary Lysine Requirement of Juvenile Yellowtail Flounder Pleuronectes ferrugineus

  • Kim, Jeong-Dae;Lall, Santosh P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1777-1781
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    • 2003
  • The lysine requirements of juvenile yellowtail flounder (Pleuronectes ferrugineus) having 19.5 g initial body weight were estimated by feeding six practical-type diets containing graded levels of lysine (1.21 to 2.69% of dry diet). Dietary amino acid profile simulated that of whole body of yellowtail flounder. Most of amino acids in the diets were provided by corn gluten meal, herring meal and gelatin. Protein efficiency ratio (PER) improved significantly until lysine level increased up to 2.1% (4.3% of protein). Same trend was observed in feed:gain ratio (FGR) which maintained constant in fish groups fed diets containing lysine above 2.1%. The highest nitrogen gain (0.34 g/fish) in whole body was found in fish fed 2.1% lysine, though the value was not different from those of fish fed above the level of lysine. Fish fed 2.1% lysine also showed the best nitrogen retention efficiency of 24.6%. The broken-line analysis of protein efficiency ratio and body nitrogen gain against dietary lysine level yielded an estimated lysine requirement of 2.2% (4.5% of protein) and 2.3% (4.7% of protein), respectively.